Experiment: R2A with Polymyxin B sulfate 0.016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS01180 and CA265_RS01185 are separated by 135 nucleotides CA265_RS01185 and CA265_RS01190 are separated by 24 nucleotides CA265_RS01190 and CA265_RS01195 are separated by 18 nucleotides CA265_RS01195 and CA265_RS01200 are separated by 34 nucleotides
CA265_RS01180: CA265_RS01180 - riboflavin biosynthesis protein, at 297,334 to 297,930
_RS01180
CA265_RS01185: CA265_RS01185 - hypothetical protein, at 298,066 to 298,494
_RS01185
CA265_RS01190: CA265_RS01190 - hypothetical protein, at 298,519 to 298,962
_RS01190
CA265_RS01195: CA265_RS01195 - hypothetical protein, at 298,981 to 299,394
_RS01195
CA265_RS01200: CA265_RS01200 - hypothetical protein, at 299,429 to 299,950
_RS01200
Position (kb)
298
299 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 297.561 kb on + strand, within CA265_RS01180 at 297.582 kb on + strand, within CA265_RS01180 at 297.619 kb on + strand, within CA265_RS01180 at 297.619 kb on + strand, within CA265_RS01180 at 297.624 kb on - strand, within CA265_RS01180 at 297.650 kb on - strand, within CA265_RS01180 at 297.702 kb on + strand, within CA265_RS01180 at 297.741 kb on + strand, within CA265_RS01180 at 297.742 kb on - strand, within CA265_RS01180 at 297.751 kb on - strand, within CA265_RS01180 at 297.752 kb on + strand, within CA265_RS01180 at 297.752 kb on + strand, within CA265_RS01180 at 297.753 kb on - strand, within CA265_RS01180 at 297.755 kb on + strand, within CA265_RS01180 at 297.756 kb on - strand, within CA265_RS01180 at 297.815 kb on + strand, within CA265_RS01180 at 297.815 kb on + strand, within CA265_RS01180 at 297.820 kb on - strand, within CA265_RS01180 at 297.895 kb on + strand at 297.906 kb on - strand at 297.906 kb on - strand at 297.907 kb on + strand at 297.907 kb on + strand at 297.908 kb on - strand at 297.949 kb on - strand at 298.057 kb on - strand at 298.081 kb on + strand at 298.081 kb on + strand at 298.085 kb on + strand at 298.085 kb on + strand at 298.086 kb on - strand at 298.230 kb on + strand, within CA265_RS01185 at 298.231 kb on - strand, within CA265_RS01185 at 298.271 kb on + strand, within CA265_RS01185 at 298.272 kb on - strand, within CA265_RS01185 at 298.272 kb on - strand, within CA265_RS01185 at 298.272 kb on - strand, within CA265_RS01185 at 298.407 kb on + strand, within CA265_RS01185 at 298.482 kb on + strand at 298.484 kb on + strand at 298.531 kb on - strand at 298.532 kb on + strand at 298.563 kb on + strand at 298.575 kb on + strand, within CA265_RS01190 at 298.583 kb on + strand, within CA265_RS01190 at 298.584 kb on - strand, within CA265_RS01190 at 298.632 kb on - strand, within CA265_RS01190 at 298.634 kb on + strand, within CA265_RS01190 at 298.664 kb on - strand, within CA265_RS01190 at 298.678 kb on - strand, within CA265_RS01190 at 298.691 kb on + strand, within CA265_RS01190 at 298.692 kb on - strand, within CA265_RS01190 at 298.721 kb on + strand, within CA265_RS01190 at 298.743 kb on - strand, within CA265_RS01190 at 298.767 kb on + strand, within CA265_RS01190 at 298.768 kb on - strand, within CA265_RS01190 at 298.768 kb on - strand, within CA265_RS01190 at 298.769 kb on + strand, within CA265_RS01190 at 298.769 kb on + strand, within CA265_RS01190 at 298.837 kb on - strand, within CA265_RS01190 at 298.867 kb on - strand, within CA265_RS01190 at 298.949 kb on - strand at 298.982 kb on + strand at 299.033 kb on - strand, within CA265_RS01195 at 299.045 kb on - strand, within CA265_RS01195 at 299.078 kb on + strand, within CA265_RS01195 at 299.078 kb on + strand, within CA265_RS01195 at 299.082 kb on + strand, within CA265_RS01195 at 299.107 kb on - strand, within CA265_RS01195 at 299.151 kb on + strand, within CA265_RS01195 at 299.152 kb on - strand, within CA265_RS01195 at 299.186 kb on + strand, within CA265_RS01195 at 299.186 kb on + strand, within CA265_RS01195 at 299.250 kb on + strand, within CA265_RS01195 at 299.281 kb on + strand, within CA265_RS01195 at 299.282 kb on - strand, within CA265_RS01195 at 299.282 kb on - strand, within CA265_RS01195 at 299.292 kb on + strand, within CA265_RS01195 at 299.368 kb on - strand at 299.378 kb on - strand at 299.406 kb on + strand at 299.407 kb on - strand at 299.427 kb on - strand at 299.431 kb on - strand at 299.441 kb on - strand at 299.499 kb on + strand, within CA265_RS01200 at 299.612 kb on + strand, within CA265_RS01200 at 299.619 kb on - strand, within CA265_RS01200 at 299.619 kb on - strand, within CA265_RS01200 at 299.698 kb on + strand, within CA265_RS01200 at 299.730 kb on - strand, within CA265_RS01200 at 299.754 kb on + strand at 299.755 kb on - strand, within CA265_RS01200 at 299.755 kb on - strand, within CA265_RS01200 at 299.757 kb on + strand, within CA265_RS01200 at 299.757 kb on + strand, within CA265_RS01200 at 299.758 kb on - strand, within CA265_RS01200 at 299.764 kb on + strand, within CA265_RS01200 at 299.795 kb on - strand, within CA265_RS01200 at 299.810 kb on - strand, within CA265_RS01200 at 299.876 kb on + strand, within CA265_RS01200 at 299.877 kb on - strand, within CA265_RS01200
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.016 mg/ml remove 297,561 + CA265_RS01180 0.38 +0.5 297,582 + CA265_RS01180 0.42 +0.0 297,619 + CA265_RS01180 0.48 +0.4 297,619 + CA265_RS01180 0.48 +0.5 297,624 - CA265_RS01180 0.49 +0.4 297,650 - CA265_RS01180 0.53 -0.5 297,702 + CA265_RS01180 0.62 +0.4 297,741 + CA265_RS01180 0.68 +0.2 297,742 - CA265_RS01180 0.68 +1.0 297,751 - CA265_RS01180 0.70 +0.6 297,752 + CA265_RS01180 0.70 -0.6 297,752 + CA265_RS01180 0.70 +0.7 297,753 - CA265_RS01180 0.70 +0.0 297,755 + CA265_RS01180 0.71 +0.6 297,756 - CA265_RS01180 0.71 +0.2 297,815 + CA265_RS01180 0.81 -0.5 297,815 + CA265_RS01180 0.81 +0.2 297,820 - CA265_RS01180 0.81 -0.1 297,895 + -0.8 297,906 - -0.4 297,906 - +0.2 297,907 + +0.7 297,907 + +0.9 297,908 - +0.2 297,949 - +0.4 298,057 - +0.3 298,081 + +0.6 298,081 + +0.4 298,085 + +0.2 298,085 + +0.9 298,086 - -1.3 298,230 + CA265_RS01185 0.38 +0.1 298,231 - CA265_RS01185 0.38 +0.8 298,271 + CA265_RS01185 0.48 +0.7 298,272 - CA265_RS01185 0.48 -1.4 298,272 - CA265_RS01185 0.48 +0.6 298,272 - CA265_RS01185 0.48 -1.1 298,407 + CA265_RS01185 0.79 +0.2 298,482 + +0.4 298,484 + +0.3 298,531 - +1.2 298,532 + +0.6 298,563 + +0.2 298,575 + CA265_RS01190 0.13 +0.3 298,583 + CA265_RS01190 0.14 +0.3 298,584 - CA265_RS01190 0.15 +0.5 298,632 - CA265_RS01190 0.25 +0.5 298,634 + CA265_RS01190 0.26 +0.4 298,664 - CA265_RS01190 0.33 +0.8 298,678 - CA265_RS01190 0.36 +1.3 298,691 + CA265_RS01190 0.39 -1.2 298,692 - CA265_RS01190 0.39 +0.5 298,721 + CA265_RS01190 0.45 +1.2 298,743 - CA265_RS01190 0.50 +0.1 298,767 + CA265_RS01190 0.56 -1.3 298,768 - CA265_RS01190 0.56 +0.8 298,768 - CA265_RS01190 0.56 +0.3 298,769 + CA265_RS01190 0.56 -0.4 298,769 + CA265_RS01190 0.56 -0.5 298,837 - CA265_RS01190 0.72 +0.7 298,867 - CA265_RS01190 0.78 +0.5 298,949 - +2.3 298,982 + +0.0 299,033 - CA265_RS01195 0.13 +2.9 299,045 - CA265_RS01195 0.15 +1.9 299,078 + CA265_RS01195 0.23 -0.8 299,078 + CA265_RS01195 0.23 +0.6 299,082 + CA265_RS01195 0.24 +0.1 299,107 - CA265_RS01195 0.30 +1.9 299,151 + CA265_RS01195 0.41 -0.1 299,152 - CA265_RS01195 0.41 +2.0 299,186 + CA265_RS01195 0.50 -0.5 299,186 + CA265_RS01195 0.50 +1.7 299,250 + CA265_RS01195 0.65 +0.4 299,281 + CA265_RS01195 0.72 +0.5 299,282 - CA265_RS01195 0.73 +2.5 299,282 - CA265_RS01195 0.73 +2.6 299,292 + CA265_RS01195 0.75 -2.3 299,368 - +2.2 299,378 - +2.0 299,406 + +1.0 299,407 - +2.8 299,427 - +2.1 299,431 - +1.6 299,441 - +2.0 299,499 + CA265_RS01200 0.13 -0.6 299,612 + CA265_RS01200 0.35 +0.3 299,619 - CA265_RS01200 0.36 +3.0 299,619 - CA265_RS01200 0.36 +2.5 299,698 + CA265_RS01200 0.52 +1.0 299,730 - CA265_RS01200 0.58 +2.6 299,754 + +1.7 299,755 - CA265_RS01200 0.62 +1.4 299,755 - CA265_RS01200 0.62 +2.3 299,757 + CA265_RS01200 0.63 +0.7 299,757 + CA265_RS01200 0.63 +0.3 299,758 - CA265_RS01200 0.63 +2.9 299,764 + CA265_RS01200 0.64 +0.7 299,795 - CA265_RS01200 0.70 +2.4 299,810 - CA265_RS01200 0.73 +1.2 299,876 + CA265_RS01200 0.86 +1.5 299,877 - CA265_RS01200 0.86 +2.8
Or see this region's nucleotide sequence