Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS09735 and RR42_RS09740 are separated by 4 nucleotides RR42_RS09740 and RR42_RS09745 are separated by 269 nucleotides
RR42_RS09735: RR42_RS09735 - multidrug ABC transporter ATP-binding protein, at 2,071,568 to 2,074,351
_RS09735
RR42_RS09740: RR42_RS09740 - hemolysin secretion protein D, at 2,074,356 to 2,075,342
_RS09740
RR42_RS09745: RR42_RS09745 - alcohol dehydrogenase, at 2,075,612 to 2,076,619
_RS09745
Position (kb)
2074
2075
2076 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2073.487 kb on + strand, within RR42_RS09735 at 2073.610 kb on + strand, within RR42_RS09735 at 2073.611 kb on - strand, within RR42_RS09735 at 2073.611 kb on - strand, within RR42_RS09735 at 2073.612 kb on + strand, within RR42_RS09735 at 2073.613 kb on - strand, within RR42_RS09735 at 2073.613 kb on - strand, within RR42_RS09735 at 2073.613 kb on - strand, within RR42_RS09735 at 2073.731 kb on - strand, within RR42_RS09735 at 2073.732 kb on + strand, within RR42_RS09735 at 2073.732 kb on + strand, within RR42_RS09735 at 2073.733 kb on - strand, within RR42_RS09735 at 2073.733 kb on - strand, within RR42_RS09735 at 2073.757 kb on + strand, within RR42_RS09735 at 2073.757 kb on + strand, within RR42_RS09735 at 2073.757 kb on + strand, within RR42_RS09735 at 2074.204 kb on + strand at 2074.204 kb on + strand at 2074.204 kb on + strand at 2074.205 kb on - strand at 2074.290 kb on + strand at 2074.460 kb on - strand, within RR42_RS09740 at 2074.501 kb on + strand, within RR42_RS09740 at 2074.501 kb on + strand, within RR42_RS09740 at 2074.502 kb on - strand, within RR42_RS09740 at 2074.502 kb on - strand, within RR42_RS09740 at 2074.537 kb on + strand, within RR42_RS09740 at 2074.538 kb on - strand, within RR42_RS09740 at 2074.681 kb on + strand, within RR42_RS09740 at 2074.681 kb on + strand, within RR42_RS09740 at 2074.681 kb on + strand, within RR42_RS09740 at 2074.681 kb on + strand, within RR42_RS09740 at 2074.681 kb on + strand, within RR42_RS09740 at 2074.681 kb on + strand, within RR42_RS09740 at 2074.681 kb on + strand, within RR42_RS09740 at 2074.682 kb on - strand, within RR42_RS09740 at 2074.682 kb on - strand, within RR42_RS09740 at 2074.682 kb on - strand, within RR42_RS09740 at 2074.682 kb on - strand, within RR42_RS09740 at 2074.682 kb on - strand, within RR42_RS09740 at 2074.686 kb on - strand, within RR42_RS09740 at 2074.686 kb on - strand, within RR42_RS09740 at 2074.781 kb on + strand, within RR42_RS09740 at 2074.957 kb on + strand, within RR42_RS09740 at 2075.095 kb on - strand, within RR42_RS09740 at 2075.095 kb on - strand, within RR42_RS09740 at 2075.183 kb on - strand, within RR42_RS09740 at 2075.240 kb on - strand, within RR42_RS09740 at 2075.240 kb on - strand, within RR42_RS09740 at 2075.240 kb on - strand, within RR42_RS09740 at 2075.240 kb on - strand, within RR42_RS09740 at 2075.310 kb on + strand at 2075.310 kb on + strand at 2075.311 kb on - strand at 2075.319 kb on - strand at 2075.479 kb on - strand at 2075.479 kb on - strand at 2075.554 kb on + strand at 2075.555 kb on - strand at 2075.555 kb on - strand at 2075.555 kb on - strand at 2075.555 kb on - strand at 2075.557 kb on + strand at 2075.558 kb on - strand at 2075.558 kb on - strand at 2075.558 kb on - strand at 2075.558 kb on - strand at 2075.558 kb on - strand at 2075.587 kb on + strand at 2075.587 kb on + strand at 2075.588 kb on - strand at 2075.827 kb on + strand, within RR42_RS09745 at 2076.097 kb on - strand, within RR42_RS09745 at 2076.097 kb on - strand, within RR42_RS09745 at 2076.198 kb on + strand, within RR42_RS09745 at 2076.198 kb on + strand, within RR42_RS09745 at 2076.212 kb on - strand, within RR42_RS09745 at 2076.222 kb on + strand, within RR42_RS09745 at 2076.222 kb on + strand, within RR42_RS09745 at 2076.222 kb on + strand, within RR42_RS09745 at 2076.243 kb on + strand, within RR42_RS09745 at 2076.252 kb on + strand, within RR42_RS09745 at 2076.268 kb on + strand, within RR42_RS09745 at 2076.269 kb on - strand, within RR42_RS09745 at 2076.322 kb on - strand, within RR42_RS09745 at 2076.322 kb on - strand, within RR42_RS09745
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 2,073,487 + RR42_RS09735 0.69 +1.5 2,073,610 + RR42_RS09735 0.73 +0.1 2,073,611 - RR42_RS09735 0.73 -0.5 2,073,611 - RR42_RS09735 0.73 +0.2 2,073,612 + RR42_RS09735 0.73 -0.4 2,073,613 - RR42_RS09735 0.73 +1.2 2,073,613 - RR42_RS09735 0.73 +1.1 2,073,613 - RR42_RS09735 0.73 -1.1 2,073,731 - RR42_RS09735 0.78 -0.6 2,073,732 + RR42_RS09735 0.78 -0.4 2,073,732 + RR42_RS09735 0.78 +0.2 2,073,733 - RR42_RS09735 0.78 +0.9 2,073,733 - RR42_RS09735 0.78 +0.6 2,073,757 + RR42_RS09735 0.79 -0.1 2,073,757 + RR42_RS09735 0.79 -0.1 2,073,757 + RR42_RS09735 0.79 +0.8 2,074,204 + -0.9 2,074,204 + -0.4 2,074,204 + -0.1 2,074,205 - -0.4 2,074,290 + -2.4 2,074,460 - RR42_RS09740 0.11 -0.2 2,074,501 + RR42_RS09740 0.15 +1.2 2,074,501 + RR42_RS09740 0.15 -0.7 2,074,502 - RR42_RS09740 0.15 -0.4 2,074,502 - RR42_RS09740 0.15 +0.1 2,074,537 + RR42_RS09740 0.18 -0.6 2,074,538 - RR42_RS09740 0.18 +0.8 2,074,681 + RR42_RS09740 0.33 -0.7 2,074,681 + RR42_RS09740 0.33 -0.1 2,074,681 + RR42_RS09740 0.33 +0.6 2,074,681 + RR42_RS09740 0.33 -0.1 2,074,681 + RR42_RS09740 0.33 -0.7 2,074,681 + RR42_RS09740 0.33 +0.9 2,074,681 + RR42_RS09740 0.33 +1.0 2,074,682 - RR42_RS09740 0.33 -0.2 2,074,682 - RR42_RS09740 0.33 -1.8 2,074,682 - RR42_RS09740 0.33 +0.7 2,074,682 - RR42_RS09740 0.33 +1.2 2,074,682 - RR42_RS09740 0.33 -1.5 2,074,686 - RR42_RS09740 0.33 -1.2 2,074,686 - RR42_RS09740 0.33 +1.1 2,074,781 + RR42_RS09740 0.43 -0.1 2,074,957 + RR42_RS09740 0.61 +1.0 2,075,095 - RR42_RS09740 0.75 -0.2 2,075,095 - RR42_RS09740 0.75 +0.3 2,075,183 - RR42_RS09740 0.84 +0.2 2,075,240 - RR42_RS09740 0.90 +0.1 2,075,240 - RR42_RS09740 0.90 +1.4 2,075,240 - RR42_RS09740 0.90 +0.1 2,075,240 - RR42_RS09740 0.90 +1.0 2,075,310 + -0.2 2,075,310 + -0.1 2,075,311 - -2.3 2,075,319 - -0.1 2,075,479 - -0.6 2,075,479 - -0.3 2,075,554 + -0.3 2,075,555 - -1.8 2,075,555 - -0.3 2,075,555 - +0.2 2,075,555 - -0.3 2,075,557 + -2.8 2,075,558 - +0.2 2,075,558 - -0.5 2,075,558 - +0.2 2,075,558 - -0.5 2,075,558 - -1.4 2,075,587 + -0.1 2,075,587 + -0.2 2,075,588 - -0.6 2,075,827 + RR42_RS09745 0.21 +0.4 2,076,097 - RR42_RS09745 0.48 -0.7 2,076,097 - RR42_RS09745 0.48 -0.1 2,076,198 + RR42_RS09745 0.58 -1.0 2,076,198 + RR42_RS09745 0.58 -1.0 2,076,212 - RR42_RS09745 0.60 -0.4 2,076,222 + RR42_RS09745 0.61 -1.4 2,076,222 + RR42_RS09745 0.61 -1.0 2,076,222 + RR42_RS09745 0.61 -1.7 2,076,243 + RR42_RS09745 0.63 +0.1 2,076,252 + RR42_RS09745 0.63 -0.3 2,076,268 + RR42_RS09745 0.65 -1.0 2,076,269 - RR42_RS09745 0.65 +0.1 2,076,322 - RR42_RS09745 0.70 -0.9 2,076,322 - RR42_RS09745 0.70 +1.5
Or see this region's nucleotide sequence