Strain Fitness in Methanococcus maripaludis JJ around MMJJ_RS01155

Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 Vitamin & Casamino Acid FREE

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMJJ_RS01140 and MMJJ_RS01150 are separated by 331 nucleotidesMMJJ_RS01150 and MMJJ_RS01155 are separated by 35 nucleotidesMMJJ_RS01155 and MMJJ_RS01160 are separated by 271 nucleotides MMJJ_RS01140: MMJJ_RS01140 - helix-turn-helix domain-containing protein, at 222,633 to 222,836 _RS01140 MMJJ_RS01150: MMJJ_RS01150 - hypothetical protein, at 223,168 to 223,587 _RS01150 MMJJ_RS01155: MMJJ_RS01155 - hypothetical protein, at 223,623 to 224,075 _RS01155 MMJJ_RS01160: MMJJ_RS01160 - AAA family ATPase, at 224,347 to 225,390 _RS01160 Position (kb) 223 224 225Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 222.628 kb on - strandat 222.629 kb on + strandat 222.661 kb on - strand, within MMJJ_RS01140at 222.662 kb on + strand, within MMJJ_RS01140at 222.675 kb on - strand, within MMJJ_RS01140at 222.675 kb on - strand, within MMJJ_RS01140at 222.705 kb on - strand, within MMJJ_RS01140at 222.708 kb on + strand, within MMJJ_RS01140at 222.725 kb on - strand, within MMJJ_RS01140at 222.737 kb on + strand, within MMJJ_RS01140at 222.815 kb on + strand, within MMJJ_RS01140at 222.815 kb on + strand, within MMJJ_RS01140at 222.894 kb on - strandat 222.910 kb on - strandat 222.910 kb on - strandat 223.030 kb on - strandat 223.031 kb on + strandat 223.098 kb on + strandat 223.137 kb on - strandat 223.144 kb on - strandat 223.145 kb on + strandat 223.179 kb on - strandat 223.179 kb on - strandat 223.184 kb on + strandat 223.184 kb on + strandat 223.258 kb on - strand, within MMJJ_RS01150at 223.259 kb on + strand, within MMJJ_RS01150at 223.259 kb on + strand, within MMJJ_RS01150at 223.270 kb on - strand, within MMJJ_RS01150at 223.346 kb on - strand, within MMJJ_RS01150at 223.492 kb on + strand, within MMJJ_RS01150at 223.620 kb on - strandat 223.620 kb on - strandat 223.647 kb on + strandat 223.649 kb on - strandat 223.649 kb on - strandat 223.649 kb on - strandat 223.650 kb on + strandat 223.651 kb on - strandat 223.651 kb on - strandat 223.652 kb on + strandat 223.652 kb on + strandat 223.652 kb on + strandat 223.652 kb on + strandat 223.652 kb on + strandat 223.652 kb on + strandat 223.652 kb on + strandat 223.652 kb on + strandat 223.652 kb on + strandat 223.652 kb on + strandat 223.652 kb on + strandat 223.656 kb on + strandat 223.656 kb on + strandat 223.750 kb on - strand, within MMJJ_RS01155at 223.782 kb on - strand, within MMJJ_RS01155at 223.929 kb on + strand, within MMJJ_RS01155at 223.944 kb on + strand, within MMJJ_RS01155at 223.950 kb on - strand, within MMJJ_RS01155at 223.952 kb on - strand, within MMJJ_RS01155at 224.035 kb on - strandat 224.060 kb on + strandat 224.081 kb on - strandat 224.222 kb on - strandat 224.223 kb on + strandat 224.265 kb on + strandat 224.267 kb on - strandat 224.268 kb on + strandat 224.269 kb on - strandat 224.271 kb on - strandat 224.272 kb on + strandat 224.272 kb on + strandat 224.272 kb on + strandat 224.278 kb on - strandat 224.279 kb on + strandat 224.279 kb on + strandat 224.306 kb on + strandat 224.355 kb on + strandat 224.361 kb on + strandat 224.380 kb on - strandat 224.591 kb on - strand, within MMJJ_RS01160at 224.688 kb on - strand, within MMJJ_RS01160at 224.689 kb on + strand, within MMJJ_RS01160at 224.717 kb on - strand, within MMJJ_RS01160at 224.753 kb on - strand, within MMJJ_RS01160at 224.753 kb on - strand, within MMJJ_RS01160at 224.753 kb on - strand, within MMJJ_RS01160at 224.753 kb on - strand, within MMJJ_RS01160at 224.753 kb on - strand, within MMJJ_RS01160at 224.753 kb on - strand, within MMJJ_RS01160at 224.754 kb on + strand, within MMJJ_RS01160at 224.754 kb on + strand, within MMJJ_RS01160at 224.754 kb on + strand, within MMJJ_RS01160at 224.754 kb on + strand, within MMJJ_RS01160at 224.754 kb on + strand, within MMJJ_RS01160at 224.755 kb on - strand, within MMJJ_RS01160at 224.755 kb on - strand, within MMJJ_RS01160at 224.755 kb on - strand, within MMJJ_RS01160at 224.756 kb on + strand, within MMJJ_RS01160at 224.756 kb on + strand, within MMJJ_RS01160at 224.756 kb on + strand, within MMJJ_RS01160at 224.756 kb on + strand, within MMJJ_RS01160at 224.756 kb on + strand, within MMJJ_RS01160at 224.761 kb on + strand, within MMJJ_RS01160at 224.761 kb on + strand, within MMJJ_RS01160at 224.800 kb on + strand, within MMJJ_RS01160at 224.845 kb on - strand, within MMJJ_RS01160at 224.859 kb on - strand, within MMJJ_RS01160at 224.859 kb on - strand, within MMJJ_RS01160at 224.869 kb on - strand, within MMJJ_RS01160at 224.869 kb on - strand, within MMJJ_RS01160at 224.870 kb on + strand, within MMJJ_RS01160at 224.870 kb on + strand, within MMJJ_RS01160at 224.942 kb on - strand, within MMJJ_RS01160at 224.942 kb on - strand, within MMJJ_RS01160at 224.976 kb on - strand, within MMJJ_RS01160at 224.976 kb on - strand, within MMJJ_RS01160at 224.977 kb on + strand, within MMJJ_RS01160at 225.020 kb on + strand, within MMJJ_RS01160at 225.028 kb on - strand, within MMJJ_RS01160at 225.030 kb on - strand, within MMJJ_RS01160at 225.030 kb on - strand, within MMJJ_RS01160at 225.030 kb on - strand, within MMJJ_RS01160at 225.030 kb on - strand, within MMJJ_RS01160at 225.031 kb on + strand, within MMJJ_RS01160at 225.031 kb on + strand, within MMJJ_RS01160at 225.031 kb on + strand, within MMJJ_RS01160at 225.031 kb on + strand, within MMJJ_RS01160at 225.031 kb on + strand, within MMJJ_RS01160at 225.031 kb on + strand, within MMJJ_RS01160at 225.032 kb on - strand, within MMJJ_RS01160at 225.032 kb on - strand, within MMJJ_RS01160at 225.032 kb on - strand, within MMJJ_RS01160at 225.032 kb on - strand, within MMJJ_RS01160at 225.032 kb on - strand, within MMJJ_RS01160at 225.032 kb on - strand, within MMJJ_RS01160at 225.032 kb on - strand, within MMJJ_RS01160at 225.033 kb on + strand, within MMJJ_RS01160at 225.033 kb on + strand, within MMJJ_RS01160at 225.033 kb on + strand, within MMJJ_RS01160at 225.033 kb on + strand, within MMJJ_RS01160at 225.033 kb on + strand, within MMJJ_RS01160at 225.033 kb on + strand, within MMJJ_RS01160at 225.033 kb on + strand, within MMJJ_RS01160at 225.034 kb on - strand, within MMJJ_RS01160at 225.034 kb on - strand, within MMJJ_RS01160at 225.034 kb on - strand, within MMJJ_RS01160at 225.034 kb on - strand, within MMJJ_RS01160at 225.034 kb on - strand, within MMJJ_RS01160at 225.035 kb on + strand, within MMJJ_RS01160at 225.035 kb on + strand, within MMJJ_RS01160at 225.039 kb on - strand, within MMJJ_RS01160at 225.039 kb on - strand, within MMJJ_RS01160at 225.051 kb on - strand, within MMJJ_RS01160at 225.055 kb on + strand, within MMJJ_RS01160

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Per-strain Table

Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 Vitamin & Casamino Acid FREE
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222,628 - -2.0
222,629 + +0.4
222,661 - MMJJ_RS01140 0.14 -3.2
222,662 + MMJJ_RS01140 0.14 +1.7
222,675 - MMJJ_RS01140 0.21 +0.9
222,675 - MMJJ_RS01140 0.21 -2.8
222,705 - MMJJ_RS01140 0.35 +1.2
222,708 + MMJJ_RS01140 0.37 -1.7
222,725 - MMJJ_RS01140 0.45 -2.3
222,737 + MMJJ_RS01140 0.51 -1.1
222,815 + MMJJ_RS01140 0.89 -2.4
222,815 + MMJJ_RS01140 0.89 +1.6
222,894 - -0.7
222,910 - -1.4
222,910 - -2.8
223,030 - +1.0
223,031 + -3.1
223,098 + -0.9
223,137 - +0.2
223,144 - -1.2
223,145 + -0.9
223,179 - +0.0
223,179 - +0.2
223,184 + +1.0
223,184 + -1.2
223,258 - MMJJ_RS01150 0.21 -0.9
223,259 + MMJJ_RS01150 0.22 -3.6
223,259 + MMJJ_RS01150 0.22 +0.7
223,270 - MMJJ_RS01150 0.24 -0.6
223,346 - MMJJ_RS01150 0.42 +0.7
223,492 + MMJJ_RS01150 0.77 +0.2
223,620 - +0.2
223,620 - -0.2
223,647 + -1.4
223,649 - +0.6
223,649 - -0.6
223,649 - +0.8
223,650 + +2.3
223,651 - -1.6
223,651 - +2.6
223,652 + -1.3
223,652 + -0.9
223,652 + +0.6
223,652 + -1.5
223,652 + +1.5
223,652 + +0.9
223,652 + -0.3
223,652 + +2.9
223,652 + -2.3
223,652 + +1.4
223,652 + +2.5
223,656 + +2.0
223,656 + -0.1
223,750 - MMJJ_RS01155 0.28 +0.5
223,782 - MMJJ_RS01155 0.35 -1.3
223,929 + MMJJ_RS01155 0.68 +0.7
223,944 + MMJJ_RS01155 0.71 +1.0
223,950 - MMJJ_RS01155 0.72 +2.4
223,952 - MMJJ_RS01155 0.73 +1.2
224,035 - +0.8
224,060 + -2.0
224,081 - -1.6
224,222 - +0.7
224,223 + -0.9
224,265 + -0.5
224,267 - -1.4
224,268 + +2.8
224,269 - -0.9
224,271 - -1.1
224,272 + +1.9
224,272 + -3.5
224,272 + -3.9
224,278 - +1.1
224,279 + -0.0
224,279 + -2.6
224,306 + -2.6
224,355 + -1.8
224,361 + -1.0
224,380 - +2.2
224,591 - MMJJ_RS01160 0.23 +2.8
224,688 - MMJJ_RS01160 0.33 +1.7
224,689 + MMJJ_RS01160 0.33 +1.3
224,717 - MMJJ_RS01160 0.35 -0.0
224,753 - MMJJ_RS01160 0.39 +3.0
224,753 - MMJJ_RS01160 0.39 +0.2
224,753 - MMJJ_RS01160 0.39 -0.9
224,753 - MMJJ_RS01160 0.39 -1.4
224,753 - MMJJ_RS01160 0.39 +1.4
224,753 - MMJJ_RS01160 0.39 -3.0
224,754 + MMJJ_RS01160 0.39 +1.7
224,754 + MMJJ_RS01160 0.39 +1.8
224,754 + MMJJ_RS01160 0.39 +1.6
224,754 + MMJJ_RS01160 0.39 +2.0
224,754 + MMJJ_RS01160 0.39 +0.2
224,755 - MMJJ_RS01160 0.39 -1.8
224,755 - MMJJ_RS01160 0.39 +3.1
224,755 - MMJJ_RS01160 0.39 +0.7
224,756 + MMJJ_RS01160 0.39 -0.7
224,756 + MMJJ_RS01160 0.39 -3.5
224,756 + MMJJ_RS01160 0.39 +1.3
224,756 + MMJJ_RS01160 0.39 +2.2
224,756 + MMJJ_RS01160 0.39 +0.4
224,761 + MMJJ_RS01160 0.40 -0.8
224,761 + MMJJ_RS01160 0.40 -1.0
224,800 + MMJJ_RS01160 0.43 -0.7
224,845 - MMJJ_RS01160 0.48 -0.2
224,859 - MMJJ_RS01160 0.49 +0.3
224,859 - MMJJ_RS01160 0.49 -1.4
224,869 - MMJJ_RS01160 0.50 +0.8
224,869 - MMJJ_RS01160 0.50 -0.8
224,870 + MMJJ_RS01160 0.50 -0.8
224,870 + MMJJ_RS01160 0.50 +1.7
224,942 - MMJJ_RS01160 0.57 +0.4
224,942 - MMJJ_RS01160 0.57 -2.4
224,976 - MMJJ_RS01160 0.60 +0.3
224,976 - MMJJ_RS01160 0.60 +1.8
224,977 + MMJJ_RS01160 0.60 +2.2
225,020 + MMJJ_RS01160 0.64 +1.9
225,028 - MMJJ_RS01160 0.65 -1.1
225,030 - MMJJ_RS01160 0.65 -0.7
225,030 - MMJJ_RS01160 0.65 -0.1
225,030 - MMJJ_RS01160 0.65 -1.9
225,030 - MMJJ_RS01160 0.65 -0.8
225,031 + MMJJ_RS01160 0.66 +1.1
225,031 + MMJJ_RS01160 0.66 -0.8
225,031 + MMJJ_RS01160 0.66 +3.0
225,031 + MMJJ_RS01160 0.66 -1.5
225,031 + MMJJ_RS01160 0.66 -1.8
225,031 + MMJJ_RS01160 0.66 -1.2
225,032 - MMJJ_RS01160 0.66 +1.5
225,032 - MMJJ_RS01160 0.66 -1.5
225,032 - MMJJ_RS01160 0.66 -0.8
225,032 - MMJJ_RS01160 0.66 +0.8
225,032 - MMJJ_RS01160 0.66 +1.0
225,032 - MMJJ_RS01160 0.66 -0.9
225,032 - MMJJ_RS01160 0.66 +1.3
225,033 + MMJJ_RS01160 0.66 -2.1
225,033 + MMJJ_RS01160 0.66 +1.1
225,033 + MMJJ_RS01160 0.66 -0.2
225,033 + MMJJ_RS01160 0.66 -2.1
225,033 + MMJJ_RS01160 0.66 -2.8
225,033 + MMJJ_RS01160 0.66 +1.7
225,033 + MMJJ_RS01160 0.66 -2.5
225,034 - MMJJ_RS01160 0.66 +0.4
225,034 - MMJJ_RS01160 0.66 +1.5
225,034 - MMJJ_RS01160 0.66 -2.0
225,034 - MMJJ_RS01160 0.66 +1.0
225,034 - MMJJ_RS01160 0.66 +0.8
225,035 + MMJJ_RS01160 0.66 -1.9
225,035 + MMJJ_RS01160 0.66 -1.3
225,039 - MMJJ_RS01160 0.66 +1.2
225,039 - MMJJ_RS01160 0.66 -1.6
225,051 - MMJJ_RS01160 0.67 -2.1
225,055 + MMJJ_RS01160 0.68 +0.2

Or see this region's nucleotide sequence