Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 Vitamin & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01060 and MMJJ_RS01065 are separated by 154 nucleotides MMJJ_RS01065 and MMJJ_RS01070 overlap by 8 nucleotides MMJJ_RS01070 and MMJJ_RS01075 are separated by 405 nucleotides MMJJ_RS01075 and MMJJ_RS01080 are separated by 9 nucleotides
MMJJ_RS01060: MMJJ_RS01060 - ABC transporter ATP-binding protein, at 206,788 to 207,492
_RS01060
MMJJ_RS01065: MMJJ_RS01065 - DUF1700 domain-containing protein, at 207,647 to 208,231
_RS01065
MMJJ_RS01070: MMJJ_RS01070 - PadR family transcriptional regulator, at 208,224 to 208,547
_RS01070
MMJJ_RS01075: MMJJ_RS01075 - hypothetical protein, at 208,953 to 209,234
_RS01075
MMJJ_RS01080: MMJJ_RS01080 - hypothetical protein, at 209,244 to 209,834
_RS01080
Position (kb) 208
209 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 207.258 kb on - strand, within MMJJ_RS01060 at 207.280 kb on + strand, within MMJJ_RS01060 at 207.295 kb on - strand, within MMJJ_RS01060 at 207.403 kb on + strand, within MMJJ_RS01060 at 207.406 kb on - strand, within MMJJ_RS01060 at 207.472 kb on + strand at 207.478 kb on - strand at 207.481 kb on - strand at 207.481 kb on - strand at 207.481 kb on - strand at 207.481 kb on - strand at 207.515 kb on - strand at 207.519 kb on + strand at 207.546 kb on + strand at 207.634 kb on - strand at 207.733 kb on - strand, within MMJJ_RS01065 at 207.733 kb on - strand, within MMJJ_RS01065 at 207.734 kb on + strand, within MMJJ_RS01065 at 207.801 kb on + strand, within MMJJ_RS01065 at 207.819 kb on + strand, within MMJJ_RS01065 at 207.850 kb on + strand, within MMJJ_RS01065 at 207.939 kb on + strand, within MMJJ_RS01065 at 207.973 kb on - strand, within MMJJ_RS01065 at 207.978 kb on + strand, within MMJJ_RS01065 at 207.980 kb on - strand, within MMJJ_RS01065 at 207.980 kb on - strand, within MMJJ_RS01065 at 207.981 kb on + strand, within MMJJ_RS01065 at 207.981 kb on + strand, within MMJJ_RS01065 at 207.981 kb on + strand, within MMJJ_RS01065 at 207.983 kb on + strand, within MMJJ_RS01065 at 207.992 kb on - strand, within MMJJ_RS01065 at 207.993 kb on + strand, within MMJJ_RS01065 at 207.993 kb on + strand, within MMJJ_RS01065 at 207.993 kb on + strand, within MMJJ_RS01065 at 208.062 kb on + strand, within MMJJ_RS01065 at 208.091 kb on + strand, within MMJJ_RS01065 at 208.154 kb on - strand, within MMJJ_RS01065 at 208.154 kb on - strand, within MMJJ_RS01065 at 208.211 kb on - strand at 208.331 kb on - strand, within MMJJ_RS01070 at 208.373 kb on + strand, within MMJJ_RS01070 at 208.420 kb on + strand, within MMJJ_RS01070 at 208.491 kb on + strand, within MMJJ_RS01070 at 208.568 kb on + strand at 208.568 kb on + strand at 208.572 kb on + strand at 208.576 kb on - strand at 208.576 kb on - strand at 208.577 kb on + strand at 208.577 kb on + strand at 208.578 kb on - strand at 208.588 kb on - strand at 208.592 kb on + strand at 208.592 kb on + strand at 208.593 kb on - strand at 208.593 kb on - strand at 208.593 kb on - strand at 208.594 kb on + strand at 208.595 kb on - strand at 208.595 kb on - strand at 208.595 kb on - strand at 208.596 kb on + strand at 208.596 kb on + strand at 208.596 kb on + strand at 208.596 kb on + strand at 208.599 kb on + strand at 208.599 kb on + strand at 208.684 kb on + strand at 208.684 kb on + strand at 208.716 kb on - strand at 208.722 kb on + strand at 208.738 kb on - strand at 208.738 kb on - strand at 208.740 kb on - strand at 208.740 kb on - strand at 208.741 kb on + strand at 208.761 kb on + strand at 208.767 kb on - strand at 208.768 kb on + strand at 208.771 kb on - strand at 208.772 kb on + strand at 208.778 kb on + strand at 208.778 kb on + strand at 208.801 kb on + strand at 208.801 kb on + strand at 208.804 kb on - strand at 208.804 kb on - strand at 208.804 kb on - strand at 208.804 kb on - strand at 208.804 kb on - strand at 208.805 kb on + strand at 208.805 kb on + strand at 208.805 kb on + strand at 208.806 kb on - strand at 208.806 kb on - strand at 208.807 kb on + strand at 208.808 kb on - strand at 208.811 kb on - strand at 208.811 kb on - strand at 208.816 kb on - strand at 208.817 kb on + strand at 208.849 kb on - strand at 208.862 kb on + strand at 208.878 kb on + strand at 208.882 kb on + strand at 208.911 kb on - strand at 208.930 kb on - strand at 208.930 kb on - strand at 208.940 kb on - strand at 208.971 kb on + strand at 208.974 kb on - strand at 208.975 kb on + strand at 208.992 kb on + strand, within MMJJ_RS01075 at 208.994 kb on - strand, within MMJJ_RS01075 at 208.997 kb on - strand, within MMJJ_RS01075 at 208.998 kb on + strand, within MMJJ_RS01075 at 209.002 kb on - strand, within MMJJ_RS01075 at 209.002 kb on - strand, within MMJJ_RS01075 at 209.003 kb on + strand, within MMJJ_RS01075 at 209.022 kb on + strand, within MMJJ_RS01075 at 209.034 kb on - strand, within MMJJ_RS01075 at 209.078 kb on - strand, within MMJJ_RS01075 at 209.078 kb on - strand, within MMJJ_RS01075 at 209.079 kb on + strand, within MMJJ_RS01075 at 209.096 kb on + strand, within MMJJ_RS01075 at 209.124 kb on + strand, within MMJJ_RS01075 at 209.124 kb on + strand, within MMJJ_RS01075 at 209.155 kb on - strand, within MMJJ_RS01075 at 209.156 kb on + strand, within MMJJ_RS01075 at 209.156 kb on + strand, within MMJJ_RS01075 at 209.156 kb on + strand, within MMJJ_RS01075 at 209.156 kb on + strand, within MMJJ_RS01075 at 209.159 kb on - strand, within MMJJ_RS01075 at 209.159 kb on - strand, within MMJJ_RS01075 at 209.159 kb on - strand, within MMJJ_RS01075 at 209.159 kb on - strand, within MMJJ_RS01075 at 209.160 kb on + strand, within MMJJ_RS01075 at 209.169 kb on - strand, within MMJJ_RS01075 at 209.170 kb on + strand, within MMJJ_RS01075 at 209.170 kb on + strand, within MMJJ_RS01075 at 209.170 kb on + strand, within MMJJ_RS01075 at 209.174 kb on - strand, within MMJJ_RS01075 at 209.175 kb on + strand, within MMJJ_RS01075 at 209.180 kb on + strand, within MMJJ_RS01075 at 209.208 kb on - strand at 209.208 kb on - strand at 209.208 kb on - strand at 209.208 kb on - strand at 209.208 kb on - strand at 209.208 kb on - strand at 209.208 kb on - strand at 209.208 kb on - strand at 209.208 kb on - strand at 209.208 kb on - strand at 209.209 kb on + strand at 209.209 kb on + strand at 209.209 kb on + strand at 209.209 kb on + strand at 209.209 kb on + strand at 209.209 kb on + strand at 209.209 kb on + strand at 209.209 kb on + strand at 209.209 kb on + strand at 209.209 kb on + strand at 209.209 kb on + strand at 209.209 kb on + strand at 209.209 kb on + strand at 209.212 kb on - strand at 209.212 kb on - strand at 209.213 kb on + strand at 209.213 kb on + strand at 209.216 kb on + strand at 209.225 kb on - strand at 209.233 kb on + strand at 209.237 kb on + strand at 209.237 kb on + strand at 209.240 kb on - strand at 209.245 kb on - strand at 209.245 kb on - strand at 209.249 kb on - strand at 209.249 kb on - strand at 209.250 kb on + strand at 209.269 kb on - strand at 209.269 kb on - strand at 209.269 kb on - strand at 209.269 kb on - strand at 209.269 kb on - strand at 209.269 kb on - strand at 209.269 kb on - strand at 209.269 kb on - strand at 209.269 kb on - strand at 209.269 kb on - strand at 209.270 kb on + strand at 209.270 kb on + strand at 209.270 kb on + strand at 209.270 kb on + strand at 209.270 kb on + strand at 209.270 kb on + strand at 209.270 kb on + strand at 209.270 kb on + strand at 209.273 kb on - strand at 209.286 kb on - strand at 209.289 kb on - strand at 209.289 kb on - strand at 209.290 kb on + strand at 209.295 kb on - strand at 209.297 kb on - strand at 209.316 kb on + strand, within MMJJ_RS01080 at 209.368 kb on + strand, within MMJJ_RS01080 at 209.372 kb on - strand, within MMJJ_RS01080 at 209.396 kb on + strand, within MMJJ_RS01080 at 209.414 kb on - strand, within MMJJ_RS01080 at 209.415 kb on + strand, within MMJJ_RS01080 at 209.415 kb on + strand, within MMJJ_RS01080 at 209.415 kb on + strand, within MMJJ_RS01080 at 209.415 kb on + strand, within MMJJ_RS01080 at 209.442 kb on - strand, within MMJJ_RS01080 at 209.442 kb on - strand, within MMJJ_RS01080 at 209.442 kb on - strand, within MMJJ_RS01080 at 209.477 kb on - strand, within MMJJ_RS01080 at 209.478 kb on + strand, within MMJJ_RS01080 at 209.480 kb on + strand, within MMJJ_RS01080 at 209.480 kb on + strand, within MMJJ_RS01080 at 209.482 kb on - strand, within MMJJ_RS01080 at 209.483 kb on + strand, within MMJJ_RS01080 at 209.502 kb on + strand, within MMJJ_RS01080
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 Vitamin & Casamino Acid FREE remove 207,258 - MMJJ_RS01060 0.67 +1.5 207,280 + MMJJ_RS01060 0.70 +2.0 207,295 - MMJJ_RS01060 0.72 +1.4 207,403 + MMJJ_RS01060 0.87 +3.0 207,406 - MMJJ_RS01060 0.88 +2.1 207,472 + -3.9 207,478 - +2.5 207,481 - +3.1 207,481 - +1.3 207,481 - -1.7 207,481 - +0.4 207,515 - +0.5 207,519 + +2.2 207,546 + -1.7 207,634 - -1.4 207,733 - MMJJ_RS01065 0.15 -2.6 207,733 - MMJJ_RS01065 0.15 -2.0 207,734 + MMJJ_RS01065 0.15 -0.7 207,801 + MMJJ_RS01065 0.26 -2.0 207,819 + MMJJ_RS01065 0.29 -2.2 207,850 + MMJJ_RS01065 0.35 +2.5 207,939 + MMJJ_RS01065 0.50 +0.2 207,973 - MMJJ_RS01065 0.56 +0.8 207,978 + MMJJ_RS01065 0.57 +0.5 207,980 - MMJJ_RS01065 0.57 +0.8 207,980 - MMJJ_RS01065 0.57 +0.6 207,981 + MMJJ_RS01065 0.57 -1.2 207,981 + MMJJ_RS01065 0.57 +3.0 207,981 + MMJJ_RS01065 0.57 +3.0 207,983 + MMJJ_RS01065 0.57 +0.9 207,992 - MMJJ_RS01065 0.59 +0.8 207,993 + MMJJ_RS01065 0.59 -0.9 207,993 + MMJJ_RS01065 0.59 -2.4 207,993 + MMJJ_RS01065 0.59 +1.4 208,062 + MMJJ_RS01065 0.71 +2.1 208,091 + MMJJ_RS01065 0.76 -1.1 208,154 - MMJJ_RS01065 0.87 -0.7 208,154 - MMJJ_RS01065 0.87 +1.0 208,211 - -0.6 208,331 - MMJJ_RS01070 0.33 -2.7 208,373 + MMJJ_RS01070 0.46 -2.1 208,420 + MMJJ_RS01070 0.60 -1.4 208,491 + MMJJ_RS01070 0.82 -3.3 208,568 + -3.4 208,568 + +0.2 208,572 + +1.2 208,576 - -0.3 208,576 - +1.5 208,577 + +1.1 208,577 + -1.0 208,578 - +1.2 208,588 - -0.7 208,592 + +1.3 208,592 + -5.5 208,593 - -0.6 208,593 - -2.9 208,593 - -0.5 208,594 + +2.2 208,595 - -2.6 208,595 - -3.2 208,595 - +0.2 208,596 + +1.8 208,596 + +3.2 208,596 + -2.5 208,596 + -1.3 208,599 + -2.6 208,599 + -0.8 208,684 + +0.4 208,684 + -1.0 208,716 - -2.0 208,722 + -1.8 208,738 - -2.5 208,738 - +1.5 208,740 - +1.0 208,740 - +1.3 208,741 + -0.8 208,761 + +0.0 208,767 - +0.2 208,768 + -0.8 208,771 - -0.7 208,772 + -2.0 208,778 + -1.5 208,778 + -2.5 208,801 + -1.5 208,801 + -0.5 208,804 - +2.0 208,804 - -0.9 208,804 - -1.5 208,804 - +1.2 208,804 - +0.6 208,805 + -1.5 208,805 + +1.3 208,805 + -2.1 208,806 - +1.6 208,806 - +1.8 208,807 + -0.1 208,808 - +2.7 208,811 - -3.0 208,811 - +3.0 208,816 - -0.6 208,817 + -1.5 208,849 - +1.7 208,862 + -2.9 208,878 + -1.4 208,882 + -2.5 208,911 - +2.9 208,930 - -0.1 208,930 - +1.8 208,940 - +2.3 208,971 + -2.1 208,974 - -1.1 208,975 + -0.9 208,992 + MMJJ_RS01075 0.14 +0.9 208,994 - MMJJ_RS01075 0.15 -1.2 208,997 - MMJJ_RS01075 0.16 +2.8 208,998 + MMJJ_RS01075 0.16 +2.2 209,002 - MMJJ_RS01075 0.17 -0.3 209,002 - MMJJ_RS01075 0.17 +0.6 209,003 + MMJJ_RS01075 0.18 -2.0 209,022 + MMJJ_RS01075 0.24 +0.6 209,034 - MMJJ_RS01075 0.29 +2.2 209,078 - MMJJ_RS01075 0.44 -1.5 209,078 - MMJJ_RS01075 0.44 -1.4 209,079 + MMJJ_RS01075 0.45 +1.1 209,096 + MMJJ_RS01075 0.51 -1.4 209,124 + MMJJ_RS01075 0.61 -0.8 209,124 + MMJJ_RS01075 0.61 -0.0 209,155 - MMJJ_RS01075 0.72 -3.1 209,156 + MMJJ_RS01075 0.72 +3.0 209,156 + MMJJ_RS01075 0.72 -1.0 209,156 + MMJJ_RS01075 0.72 -1.1 209,156 + MMJJ_RS01075 0.72 -1.1 209,159 - MMJJ_RS01075 0.73 +0.6 209,159 - MMJJ_RS01075 0.73 -0.5 209,159 - MMJJ_RS01075 0.73 -0.9 209,159 - MMJJ_RS01075 0.73 -1.0 209,160 + MMJJ_RS01075 0.73 +0.1 209,169 - MMJJ_RS01075 0.77 -1.1 209,170 + MMJJ_RS01075 0.77 -0.5 209,170 + MMJJ_RS01075 0.77 -0.1 209,170 + MMJJ_RS01075 0.77 -2.9 209,174 - MMJJ_RS01075 0.78 +0.9 209,175 + MMJJ_RS01075 0.79 +2.8 209,180 + MMJJ_RS01075 0.80 +2.5 209,208 - +2.4 209,208 - +1.4 209,208 - +1.4 209,208 - +1.6 209,208 - -1.2 209,208 - +1.8 209,208 - +1.6 209,208 - -1.6 209,208 - -0.4 209,208 - +0.6 209,209 + -0.5 209,209 + -0.8 209,209 + +1.0 209,209 + -1.6 209,209 + +1.9 209,209 + +2.6 209,209 + +1.5 209,209 + -2.0 209,209 + +2.4 209,209 + -0.5 209,209 + -0.1 209,209 + -2.8 209,209 + +1.3 209,212 - -1.7 209,212 - +2.3 209,213 + +0.8 209,213 + +0.9 209,216 + -2.9 209,225 - -1.7 209,233 + -1.9 209,237 + -2.8 209,237 + +0.9 209,240 - +2.5 209,245 - -1.9 209,245 - +1.8 209,249 - -1.2 209,249 - -2.0 209,250 + -3.4 209,269 - -1.4 209,269 - +2.8 209,269 - -1.5 209,269 - +0.6 209,269 - +2.1 209,269 - +1.8 209,269 - -1.2 209,269 - +1.0 209,269 - -1.7 209,269 - +1.0 209,270 + +1.0 209,270 + +2.0 209,270 + -1.6 209,270 + +1.6 209,270 + +0.8 209,270 + -1.1 209,270 + +1.8 209,270 + -0.8 209,273 - -2.4 209,286 - +1.3 209,289 - +0.3 209,289 - -1.8 209,290 + +1.9 209,295 - +2.2 209,297 - +0.7 209,316 + MMJJ_RS01080 0.12 +1.1 209,368 + MMJJ_RS01080 0.21 +1.0 209,372 - MMJJ_RS01080 0.22 +0.2 209,396 + MMJJ_RS01080 0.26 -0.8 209,414 - MMJJ_RS01080 0.29 -0.8 209,415 + MMJJ_RS01080 0.29 +3.5 209,415 + MMJJ_RS01080 0.29 -3.5 209,415 + MMJJ_RS01080 0.29 -1.1 209,415 + MMJJ_RS01080 0.29 -1.8 209,442 - MMJJ_RS01080 0.34 +1.1 209,442 - MMJJ_RS01080 0.34 -0.7 209,442 - MMJJ_RS01080 0.34 +0.2 209,477 - MMJJ_RS01080 0.39 +1.9 209,478 + MMJJ_RS01080 0.40 +2.8 209,480 + MMJJ_RS01080 0.40 +2.2 209,480 + MMJJ_RS01080 0.40 -1.8 209,482 - MMJJ_RS01080 0.40 -1.4 209,483 + MMJJ_RS01080 0.40 +1.8 209,502 + MMJJ_RS01080 0.44 -0.5
Or see this region's nucleotide sequence