Experiment: R2A with perchlorate 25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS20165 and CA265_RS20170 are separated by 5 nucleotides CA265_RS20170 and CA265_RS20175 overlap by 4 nucleotides CA265_RS20175 and CA265_RS20180 are separated by 10 nucleotides
CA265_RS20165: CA265_RS20165 - phosphomethylpyrimidine synthase ThiC, at 4,985,037 to 4,986,920
_RS20165
CA265_RS20170: CA265_RS20170 - hypothetical protein, at 4,986,926 to 4,987,540
_RS20170
CA265_RS20175: CA265_RS20175 - thiamine-phosphate diphosphorylase, at 4,987,537 to 4,988,175
_RS20175
CA265_RS20180: CA265_RS20180 - thiazole synthase, at 4,988,186 to 4,988,956
_RS20180
Position (kb)
4986
4987
4988 Strain fitness (log2 ratio)
-2
-1
0
1 at 4985.977 kb on + strand, within CA265_RS20165 at 4986.002 kb on - strand, within CA265_RS20165 at 4986.028 kb on + strand, within CA265_RS20165 at 4986.029 kb on - strand, within CA265_RS20165 at 4986.039 kb on + strand, within CA265_RS20165 at 4986.039 kb on + strand, within CA265_RS20165 at 4986.039 kb on + strand, within CA265_RS20165 at 4986.071 kb on + strand, within CA265_RS20165 at 4986.072 kb on - strand, within CA265_RS20165 at 4986.139 kb on - strand, within CA265_RS20165 at 4986.221 kb on + strand, within CA265_RS20165 at 4986.334 kb on + strand, within CA265_RS20165 at 4986.335 kb on - strand, within CA265_RS20165 at 4986.527 kb on - strand, within CA265_RS20165 at 4986.551 kb on + strand, within CA265_RS20165 at 4986.641 kb on - strand, within CA265_RS20165 at 4986.662 kb on + strand, within CA265_RS20165 at 4986.961 kb on + strand at 4987.060 kb on + strand, within CA265_RS20170 at 4987.114 kb on + strand, within CA265_RS20170 at 4987.115 kb on - strand, within CA265_RS20170 at 4987.115 kb on - strand, within CA265_RS20170 at 4987.133 kb on - strand, within CA265_RS20170 at 4987.207 kb on - strand, within CA265_RS20170 at 4987.233 kb on + strand, within CA265_RS20170 at 4987.234 kb on - strand, within CA265_RS20170 at 4987.263 kb on - strand, within CA265_RS20170 at 4987.263 kb on - strand, within CA265_RS20170 at 4987.307 kb on + strand, within CA265_RS20170 at 4987.474 kb on - strand, within CA265_RS20170 at 4987.560 kb on + strand at 4987.561 kb on - strand at 4987.593 kb on - strand at 4987.611 kb on - strand, within CA265_RS20175 at 4987.670 kb on + strand, within CA265_RS20175 at 4987.728 kb on + strand, within CA265_RS20175 at 4987.768 kb on + strand, within CA265_RS20175 at 4987.787 kb on + strand, within CA265_RS20175 at 4987.848 kb on + strand, within CA265_RS20175 at 4987.900 kb on + strand, within CA265_RS20175 at 4987.901 kb on - strand, within CA265_RS20175 at 4987.958 kb on + strand, within CA265_RS20175 at 4987.995 kb on - strand, within CA265_RS20175 at 4988.076 kb on + strand, within CA265_RS20175 at 4988.080 kb on + strand, within CA265_RS20175 at 4988.081 kb on - strand, within CA265_RS20175 at 4988.133 kb on - strand at 4988.185 kb on + strand at 4988.186 kb on - strand at 4988.186 kb on - strand at 4988.344 kb on + strand, within CA265_RS20180 at 4988.345 kb on - strand, within CA265_RS20180 at 4988.380 kb on - strand, within CA265_RS20180
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with perchlorate 25 mM remove 4,985,977 + CA265_RS20165 0.50 -0.5 4,986,002 - CA265_RS20165 0.51 -0.5 4,986,028 + CA265_RS20165 0.53 -0.7 4,986,029 - CA265_RS20165 0.53 +0.4 4,986,039 + CA265_RS20165 0.53 -0.4 4,986,039 + CA265_RS20165 0.53 -1.1 4,986,039 + CA265_RS20165 0.53 -1.3 4,986,071 + CA265_RS20165 0.55 -1.3 4,986,072 - CA265_RS20165 0.55 -0.0 4,986,139 - CA265_RS20165 0.58 -0.1 4,986,221 + CA265_RS20165 0.63 +0.6 4,986,334 + CA265_RS20165 0.69 -1.3 4,986,335 - CA265_RS20165 0.69 -0.2 4,986,527 - CA265_RS20165 0.79 -0.3 4,986,551 + CA265_RS20165 0.80 +0.4 4,986,641 - CA265_RS20165 0.85 -0.2 4,986,662 + CA265_RS20165 0.86 -2.0 4,986,961 + -1.2 4,987,060 + CA265_RS20170 0.22 -0.7 4,987,114 + CA265_RS20170 0.31 -0.6 4,987,115 - CA265_RS20170 0.31 -2.3 4,987,115 - CA265_RS20170 0.31 -2.3 4,987,133 - CA265_RS20170 0.34 -0.0 4,987,207 - CA265_RS20170 0.46 -1.1 4,987,233 + CA265_RS20170 0.50 -1.0 4,987,234 - CA265_RS20170 0.50 -0.4 4,987,263 - CA265_RS20170 0.55 -1.1 4,987,263 - CA265_RS20170 0.55 -0.9 4,987,307 + CA265_RS20170 0.62 -1.2 4,987,474 - CA265_RS20170 0.89 -1.1 4,987,560 + -0.2 4,987,561 - +0.3 4,987,593 - -0.2 4,987,611 - CA265_RS20175 0.12 -2.1 4,987,670 + CA265_RS20175 0.21 -0.1 4,987,728 + CA265_RS20175 0.30 +1.0 4,987,768 + CA265_RS20175 0.36 -0.2 4,987,787 + CA265_RS20175 0.39 -1.1 4,987,848 + CA265_RS20175 0.49 -1.0 4,987,900 + CA265_RS20175 0.57 -1.6 4,987,901 - CA265_RS20175 0.57 -0.7 4,987,958 + CA265_RS20175 0.66 -0.0 4,987,995 - CA265_RS20175 0.72 -0.8 4,988,076 + CA265_RS20175 0.84 -0.4 4,988,080 + CA265_RS20175 0.85 +1.1 4,988,081 - CA265_RS20175 0.85 -1.9 4,988,133 - -1.0 4,988,185 + +0.1 4,988,186 - -2.1 4,988,186 - +0.8 4,988,344 + CA265_RS20180 0.20 -0.0 4,988,345 - CA265_RS20180 0.21 +0.2 4,988,380 - CA265_RS20180 0.25 -0.9
Or see this region's nucleotide sequence