Experiment: R2A with perchlorate 25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS10470 and CA265_RS10475 are separated by 124 nucleotides CA265_RS10475 and CA265_RS10480 are separated by 48 nucleotides CA265_RS10480 and CA265_RS10485 overlap by 14 nucleotides CA265_RS10485 and CA265_RS10490 are separated by 127 nucleotides
CA265_RS10470: CA265_RS10470 - amidinotransferase, at 2,594,861 to 2,596,306
_RS10470
CA265_RS10475: CA265_RS10475 - amidinotransferase, at 2,596,431 to 2,597,345
_RS10475
CA265_RS10480: CA265_RS10480 - hypothetical protein, at 2,597,394 to 2,597,708
_RS10480
CA265_RS10485: CA265_RS10485 - hypothetical protein, at 2,597,695 to 2,597,958
_RS10485
CA265_RS10490: CA265_RS10490 - PhnA protein, at 2,598,086 to 2,598,664
_RS10490
Position (kb)
2596
2597
2598 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2595.474 kb on - strand, within CA265_RS10470 at 2595.476 kb on - strand, within CA265_RS10470 at 2595.477 kb on + strand, within CA265_RS10470 at 2595.537 kb on + strand, within CA265_RS10470 at 2595.537 kb on + strand, within CA265_RS10470 at 2595.620 kb on - strand, within CA265_RS10470 at 2595.628 kb on - strand, within CA265_RS10470 at 2595.685 kb on - strand, within CA265_RS10470 at 2595.746 kb on + strand at 2595.746 kb on + strand, within CA265_RS10470 at 2595.747 kb on - strand, within CA265_RS10470 at 2595.747 kb on - strand, within CA265_RS10470 at 2595.761 kb on - strand, within CA265_RS10470 at 2595.827 kb on + strand, within CA265_RS10470 at 2595.829 kb on + strand, within CA265_RS10470 at 2595.830 kb on - strand, within CA265_RS10470 at 2595.902 kb on - strand, within CA265_RS10470 at 2595.902 kb on - strand, within CA265_RS10470 at 2595.902 kb on - strand, within CA265_RS10470 at 2595.992 kb on - strand, within CA265_RS10470 at 2595.992 kb on - strand, within CA265_RS10470 at 2596.150 kb on - strand, within CA265_RS10470 at 2596.159 kb on - strand, within CA265_RS10470 at 2596.194 kb on + strand at 2596.246 kb on - strand at 2596.290 kb on + strand at 2596.290 kb on + strand at 2596.291 kb on - strand at 2596.301 kb on - strand at 2596.323 kb on + strand at 2596.323 kb on + strand at 2596.324 kb on - strand at 2596.325 kb on + strand at 2596.355 kb on + strand at 2596.378 kb on - strand at 2596.392 kb on - strand at 2596.401 kb on + strand at 2596.402 kb on - strand at 2596.439 kb on + strand at 2596.440 kb on - strand at 2596.448 kb on + strand at 2596.624 kb on + strand, within CA265_RS10475 at 2596.625 kb on - strand, within CA265_RS10475 at 2596.647 kb on - strand, within CA265_RS10475 at 2596.792 kb on + strand, within CA265_RS10475 at 2596.843 kb on + strand, within CA265_RS10475 at 2596.847 kb on - strand, within CA265_RS10475 at 2596.869 kb on + strand, within CA265_RS10475 at 2596.879 kb on + strand, within CA265_RS10475 at 2596.980 kb on + strand, within CA265_RS10475 at 2597.070 kb on - strand, within CA265_RS10475 at 2597.070 kb on - strand, within CA265_RS10475 at 2597.081 kb on - strand, within CA265_RS10475 at 2597.081 kb on - strand, within CA265_RS10475 at 2597.081 kb on - strand, within CA265_RS10475 at 2597.150 kb on - strand, within CA265_RS10475 at 2597.158 kb on - strand, within CA265_RS10475 at 2597.179 kb on + strand, within CA265_RS10475 at 2597.260 kb on - strand at 2597.349 kb on + strand at 2597.365 kb on - strand at 2597.544 kb on + strand, within CA265_RS10480 at 2597.544 kb on + strand, within CA265_RS10480 at 2597.577 kb on - strand, within CA265_RS10480 at 2597.577 kb on - strand, within CA265_RS10480 at 2597.586 kb on - strand, within CA265_RS10480 at 2597.608 kb on + strand, within CA265_RS10480 at 2597.609 kb on - strand, within CA265_RS10480 at 2597.696 kb on + strand at 2597.696 kb on + strand at 2597.710 kb on - strand at 2597.715 kb on + strand at 2597.718 kb on + strand at 2597.801 kb on - strand, within CA265_RS10485 at 2597.812 kb on - strand, within CA265_RS10485 at 2597.832 kb on + strand, within CA265_RS10485 at 2597.833 kb on - strand, within CA265_RS10485 at 2597.892 kb on + strand, within CA265_RS10485 at 2597.893 kb on - strand, within CA265_RS10485 at 2597.973 kb on - strand at 2598.085 kb on + strand at 2598.085 kb on + strand at 2598.086 kb on - strand at 2598.135 kb on + strand at 2598.135 kb on + strand at 2598.136 kb on - strand at 2598.156 kb on + strand, within CA265_RS10490 at 2598.165 kb on - strand, within CA265_RS10490 at 2598.277 kb on - strand, within CA265_RS10490
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with perchlorate 25 mM remove 2,595,474 - CA265_RS10470 0.42 -2.0 2,595,476 - CA265_RS10470 0.43 -2.1 2,595,477 + CA265_RS10470 0.43 -1.2 2,595,537 + CA265_RS10470 0.47 -1.2 2,595,537 + CA265_RS10470 0.47 -0.8 2,595,620 - CA265_RS10470 0.52 -1.8 2,595,628 - CA265_RS10470 0.53 -2.4 2,595,685 - CA265_RS10470 0.57 -0.6 2,595,746 + +0.0 2,595,746 + CA265_RS10470 0.61 -1.4 2,595,747 - CA265_RS10470 0.61 -2.4 2,595,747 - CA265_RS10470 0.61 -2.4 2,595,761 - CA265_RS10470 0.62 -1.8 2,595,827 + CA265_RS10470 0.67 -2.3 2,595,829 + CA265_RS10470 0.67 +0.1 2,595,830 - CA265_RS10470 0.67 -1.2 2,595,902 - CA265_RS10470 0.72 -1.5 2,595,902 - CA265_RS10470 0.72 -0.8 2,595,902 - CA265_RS10470 0.72 +0.4 2,595,992 - CA265_RS10470 0.78 -1.6 2,595,992 - CA265_RS10470 0.78 -1.4 2,596,150 - CA265_RS10470 0.89 +0.5 2,596,159 - CA265_RS10470 0.90 -1.7 2,596,194 + +0.6 2,596,246 - -1.5 2,596,290 + -1.0 2,596,290 + +1.4 2,596,291 - -0.9 2,596,301 - -1.0 2,596,323 + -0.6 2,596,323 + +0.3 2,596,324 - -0.6 2,596,325 + +0.6 2,596,355 + +1.5 2,596,378 - -2.0 2,596,392 - -1.9 2,596,401 + -0.3 2,596,402 - -0.5 2,596,439 + -1.7 2,596,440 - -1.8 2,596,448 + -1.0 2,596,624 + CA265_RS10475 0.21 -1.6 2,596,625 - CA265_RS10475 0.21 -1.3 2,596,647 - CA265_RS10475 0.24 -3.1 2,596,792 + CA265_RS10475 0.39 -1.5 2,596,843 + CA265_RS10475 0.45 -1.1 2,596,847 - CA265_RS10475 0.45 -2.0 2,596,869 + CA265_RS10475 0.48 -2.2 2,596,879 + CA265_RS10475 0.49 -1.0 2,596,980 + CA265_RS10475 0.60 +1.4 2,597,070 - CA265_RS10475 0.70 -0.8 2,597,070 - CA265_RS10475 0.70 +0.6 2,597,081 - CA265_RS10475 0.71 -1.3 2,597,081 - CA265_RS10475 0.71 -1.3 2,597,081 - CA265_RS10475 0.71 -2.0 2,597,150 - CA265_RS10475 0.79 -1.3 2,597,158 - CA265_RS10475 0.79 -1.8 2,597,179 + CA265_RS10475 0.82 -0.8 2,597,260 - -1.0 2,597,349 + -0.6 2,597,365 - +0.1 2,597,544 + CA265_RS10480 0.48 +1.1 2,597,544 + CA265_RS10480 0.48 +1.0 2,597,577 - CA265_RS10480 0.58 -0.5 2,597,577 - CA265_RS10480 0.58 +0.4 2,597,586 - CA265_RS10480 0.61 +2.4 2,597,608 + CA265_RS10480 0.68 -0.4 2,597,609 - CA265_RS10480 0.68 -0.4 2,597,696 + -0.4 2,597,696 + +0.2 2,597,710 - -0.1 2,597,715 + -0.8 2,597,718 + +0.4 2,597,801 - CA265_RS10485 0.40 +0.0 2,597,812 - CA265_RS10485 0.44 +0.8 2,597,832 + CA265_RS10485 0.52 +0.3 2,597,833 - CA265_RS10485 0.52 +0.0 2,597,892 + CA265_RS10485 0.75 +0.2 2,597,893 - CA265_RS10485 0.75 -0.2 2,597,973 - +0.1 2,598,085 + -0.5 2,598,085 + +0.2 2,598,086 - -0.4 2,598,135 + -0.6 2,598,135 + -0.6 2,598,136 - +0.5 2,598,156 + CA265_RS10490 0.12 -0.3 2,598,165 - CA265_RS10490 0.14 +0.7 2,598,277 - CA265_RS10490 0.33 +0.3
Or see this region's nucleotide sequence