Experiment: R2A with perchlorate 12.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS22975 and CA265_RS22980 are separated by 65 nucleotides CA265_RS22980 and CA265_RS22985 are separated by 188 nucleotides CA265_RS22985 and CA265_RS22990 are separated by 280 nucleotides
CA265_RS22975: CA265_RS22975 - 3-keto-alpha-glucoside 1,2-lyase (from data) , at 5,622,528 to 5,623,310
_RS22975
CA265_RS22980: CA265_RS22980 - hypothetical protein, at 5,623,376 to 5,623,693
_RS22980
CA265_RS22985: CA265_RS22985 - helicase SNF2, at 5,623,882 to 5,626,773
_RS22985
CA265_RS22990: CA265_RS22990 - hypothetical protein, at 5,627,054 to 5,628,064
_RS22990
Position (kb)
5623
5624
5625
5626
5627 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 5622.924 kb on + strand, within CA265_RS22975 at 5622.987 kb on - strand, within CA265_RS22975 at 5623.086 kb on - strand, within CA265_RS22975 at 5623.086 kb on - strand, within CA265_RS22975 at 5623.097 kb on + strand, within CA265_RS22975 at 5623.098 kb on - strand, within CA265_RS22975 at 5623.248 kb on - strand at 5623.291 kb on - strand at 5623.293 kb on + strand at 5623.293 kb on + strand at 5623.299 kb on - strand at 5623.300 kb on + strand at 5623.301 kb on - strand at 5623.344 kb on + strand at 5623.415 kb on + strand, within CA265_RS22980 at 5623.466 kb on - strand, within CA265_RS22980 at 5623.496 kb on + strand, within CA265_RS22980 at 5623.576 kb on - strand, within CA265_RS22980 at 5623.576 kb on - strand, within CA265_RS22980 at 5623.576 kb on - strand, within CA265_RS22980 at 5623.664 kb on + strand at 5623.665 kb on - strand at 5623.713 kb on + strand at 5623.717 kb on + strand at 5623.719 kb on - strand at 5623.719 kb on - strand at 5623.719 kb on - strand at 5623.721 kb on - strand at 5623.807 kb on - strand at 5623.827 kb on + strand at 5623.872 kb on + strand at 5623.894 kb on - strand at 5623.902 kb on + strand at 5623.903 kb on - strand at 5623.949 kb on - strand at 5623.990 kb on + strand at 5623.991 kb on - strand at 5624.067 kb on + strand at 5624.250 kb on - strand, within CA265_RS22985 at 5624.331 kb on + strand, within CA265_RS22985 at 5624.332 kb on - strand, within CA265_RS22985 at 5624.416 kb on + strand, within CA265_RS22985 at 5624.543 kb on + strand, within CA265_RS22985 at 5624.546 kb on + strand, within CA265_RS22985 at 5624.602 kb on + strand, within CA265_RS22985 at 5624.662 kb on + strand, within CA265_RS22985 at 5624.662 kb on + strand, within CA265_RS22985 at 5624.712 kb on - strand, within CA265_RS22985 at 5624.712 kb on - strand, within CA265_RS22985 at 5624.736 kb on + strand, within CA265_RS22985 at 5624.756 kb on - strand, within CA265_RS22985 at 5624.824 kb on - strand, within CA265_RS22985 at 5624.824 kb on - strand, within CA265_RS22985 at 5624.878 kb on - strand, within CA265_RS22985 at 5624.901 kb on + strand, within CA265_RS22985 at 5624.901 kb on + strand, within CA265_RS22985 at 5624.936 kb on - strand, within CA265_RS22985 at 5624.957 kb on + strand, within CA265_RS22985 at 5624.958 kb on - strand, within CA265_RS22985 at 5624.965 kb on - strand, within CA265_RS22985 at 5624.971 kb on - strand, within CA265_RS22985 at 5624.971 kb on - strand, within CA265_RS22985 at 5624.979 kb on - strand, within CA265_RS22985 at 5625.004 kb on - strand, within CA265_RS22985 at 5625.013 kb on - strand, within CA265_RS22985 at 5625.022 kb on - strand, within CA265_RS22985 at 5625.031 kb on + strand, within CA265_RS22985 at 5625.057 kb on + strand, within CA265_RS22985 at 5625.078 kb on - strand, within CA265_RS22985 at 5625.078 kb on - strand, within CA265_RS22985 at 5625.103 kb on - strand, within CA265_RS22985 at 5625.164 kb on - strand, within CA265_RS22985 at 5625.221 kb on + strand, within CA265_RS22985 at 5625.246 kb on + strand, within CA265_RS22985 at 5625.247 kb on - strand, within CA265_RS22985 at 5625.249 kb on - strand, within CA265_RS22985 at 5625.272 kb on - strand, within CA265_RS22985 at 5625.360 kb on + strand, within CA265_RS22985 at 5625.391 kb on + strand, within CA265_RS22985 at 5625.458 kb on + strand, within CA265_RS22985 at 5625.465 kb on + strand, within CA265_RS22985 at 5625.483 kb on + strand, within CA265_RS22985 at 5625.534 kb on + strand, within CA265_RS22985 at 5625.535 kb on - strand, within CA265_RS22985 at 5625.711 kb on + strand, within CA265_RS22985 at 5625.730 kb on - strand, within CA265_RS22985 at 5625.741 kb on - strand, within CA265_RS22985 at 5625.901 kb on + strand, within CA265_RS22985 at 5625.902 kb on - strand, within CA265_RS22985 at 5625.902 kb on - strand, within CA265_RS22985 at 5625.902 kb on - strand, within CA265_RS22985 at 5625.937 kb on - strand, within CA265_RS22985 at 5625.952 kb on - strand, within CA265_RS22985 at 5626.052 kb on + strand, within CA265_RS22985 at 5626.053 kb on - strand, within CA265_RS22985 at 5626.053 kb on - strand, within CA265_RS22985 at 5626.098 kb on - strand, within CA265_RS22985 at 5626.101 kb on + strand, within CA265_RS22985 at 5626.129 kb on - strand, within CA265_RS22985 at 5626.137 kb on + strand, within CA265_RS22985 at 5626.170 kb on + strand, within CA265_RS22985 at 5626.192 kb on + strand, within CA265_RS22985 at 5626.192 kb on + strand, within CA265_RS22985 at 5626.193 kb on - strand, within CA265_RS22985 at 5626.264 kb on + strand, within CA265_RS22985 at 5626.394 kb on - strand, within CA265_RS22985 at 5626.397 kb on + strand, within CA265_RS22985 at 5626.397 kb on + strand, within CA265_RS22985 at 5626.532 kb on + strand at 5626.587 kb on - strand at 5626.594 kb on - strand at 5626.600 kb on + strand at 5626.712 kb on + strand at 5626.734 kb on - strand at 5626.744 kb on - strand at 5626.753 kb on - strand at 5626.758 kb on + strand at 5626.769 kb on - strand at 5626.800 kb on + strand at 5626.801 kb on - strand at 5626.824 kb on + strand at 5626.829 kb on + strand at 5626.840 kb on + strand at 5626.840 kb on + strand at 5626.909 kb on + strand at 5626.972 kb on - strand at 5626.973 kb on + strand at 5626.988 kb on + strand at 5627.005 kb on + strand at 5627.012 kb on - strand at 5627.052 kb on - strand at 5627.135 kb on + strand at 5627.138 kb on - strand at 5627.209 kb on - strand, within CA265_RS22990 at 5627.253 kb on - strand, within CA265_RS22990 at 5627.263 kb on - strand, within CA265_RS22990 at 5627.350 kb on - strand, within CA265_RS22990 at 5627.357 kb on + strand, within CA265_RS22990 at 5627.357 kb on + strand, within CA265_RS22990 at 5627.359 kb on + strand, within CA265_RS22990 at 5627.437 kb on - strand, within CA265_RS22990 at 5627.447 kb on + strand, within CA265_RS22990 at 5627.447 kb on + strand, within CA265_RS22990 at 5627.447 kb on + strand, within CA265_RS22990 at 5627.448 kb on - strand, within CA265_RS22990 at 5627.448 kb on - strand, within CA265_RS22990 at 5627.518 kb on + strand, within CA265_RS22990 at 5627.542 kb on - strand, within CA265_RS22990 at 5627.615 kb on - strand, within CA265_RS22990 at 5627.615 kb on - strand, within CA265_RS22990 at 5627.623 kb on - strand, within CA265_RS22990 at 5627.624 kb on + strand, within CA265_RS22990 at 5627.625 kb on - strand, within CA265_RS22990 at 5627.625 kb on - strand, within CA265_RS22990 at 5627.626 kb on + strand, within CA265_RS22990 at 5627.626 kb on + strand, within CA265_RS22990 at 5627.750 kb on - strand, within CA265_RS22990 at 5627.750 kb on - strand, within CA265_RS22990
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with perchlorate 12.5 mM remove 5,622,924 + CA265_RS22975 0.51 +0.3 5,622,987 - CA265_RS22975 0.59 -0.5 5,623,086 - CA265_RS22975 0.71 +2.1 5,623,086 - CA265_RS22975 0.71 +0.1 5,623,097 + CA265_RS22975 0.73 +0.3 5,623,098 - CA265_RS22975 0.73 +0.2 5,623,248 - -1.3 5,623,291 - -0.1 5,623,293 + +0.4 5,623,293 + -1.2 5,623,299 - -0.1 5,623,300 + +0.1 5,623,301 - +0.7 5,623,344 + -0.6 5,623,415 + CA265_RS22980 0.12 +0.2 5,623,466 - CA265_RS22980 0.28 +1.7 5,623,496 + CA265_RS22980 0.38 -0.8 5,623,576 - CA265_RS22980 0.63 +0.3 5,623,576 - CA265_RS22980 0.63 -1.6 5,623,576 - CA265_RS22980 0.63 +0.1 5,623,664 + -0.5 5,623,665 - -2.0 5,623,713 + +0.1 5,623,717 + +0.3 5,623,719 - -3.0 5,623,719 - -0.1 5,623,719 - -1.1 5,623,721 - +2.4 5,623,807 - +0.1 5,623,827 + +0.1 5,623,872 + +0.0 5,623,894 - +0.3 5,623,902 + +0.3 5,623,903 - -0.3 5,623,949 - +1.6 5,623,990 + -0.7 5,623,991 - +1.1 5,624,067 + -0.6 5,624,250 - CA265_RS22985 0.13 +1.4 5,624,331 + CA265_RS22985 0.16 +0.5 5,624,332 - CA265_RS22985 0.16 +0.7 5,624,416 + CA265_RS22985 0.18 +3.7 5,624,543 + CA265_RS22985 0.23 +0.4 5,624,546 + CA265_RS22985 0.23 -0.1 5,624,602 + CA265_RS22985 0.25 +0.2 5,624,662 + CA265_RS22985 0.27 +1.8 5,624,662 + CA265_RS22985 0.27 +0.2 5,624,712 - CA265_RS22985 0.29 +0.5 5,624,712 - CA265_RS22985 0.29 -0.2 5,624,736 + CA265_RS22985 0.30 +0.6 5,624,756 - CA265_RS22985 0.30 +0.3 5,624,824 - CA265_RS22985 0.33 +0.0 5,624,824 - CA265_RS22985 0.33 +0.3 5,624,878 - CA265_RS22985 0.34 +2.9 5,624,901 + CA265_RS22985 0.35 +0.6 5,624,901 + CA265_RS22985 0.35 +0.5 5,624,936 - CA265_RS22985 0.36 +0.3 5,624,957 + CA265_RS22985 0.37 -0.5 5,624,958 - CA265_RS22985 0.37 -1.0 5,624,965 - CA265_RS22985 0.37 +0.4 5,624,971 - CA265_RS22985 0.38 +0.2 5,624,971 - CA265_RS22985 0.38 +0.7 5,624,979 - CA265_RS22985 0.38 -0.2 5,625,004 - CA265_RS22985 0.39 +0.8 5,625,013 - CA265_RS22985 0.39 +1.3 5,625,022 - CA265_RS22985 0.39 -1.3 5,625,031 + CA265_RS22985 0.40 +0.9 5,625,057 + CA265_RS22985 0.41 +1.2 5,625,078 - CA265_RS22985 0.41 +0.8 5,625,078 - CA265_RS22985 0.41 +0.9 5,625,103 - CA265_RS22985 0.42 +0.7 5,625,164 - CA265_RS22985 0.44 +0.9 5,625,221 + CA265_RS22985 0.46 +0.8 5,625,246 + CA265_RS22985 0.47 +1.4 5,625,247 - CA265_RS22985 0.47 -0.1 5,625,249 - CA265_RS22985 0.47 +0.1 5,625,272 - CA265_RS22985 0.48 +0.4 5,625,360 + CA265_RS22985 0.51 +1.7 5,625,391 + CA265_RS22985 0.52 +0.3 5,625,458 + CA265_RS22985 0.54 +0.4 5,625,465 + CA265_RS22985 0.55 -0.0 5,625,483 + CA265_RS22985 0.55 -0.3 5,625,534 + CA265_RS22985 0.57 +0.9 5,625,535 - CA265_RS22985 0.57 +0.5 5,625,711 + CA265_RS22985 0.63 -1.2 5,625,730 - CA265_RS22985 0.64 +0.1 5,625,741 - CA265_RS22985 0.64 +0.5 5,625,901 + CA265_RS22985 0.70 +0.2 5,625,902 - CA265_RS22985 0.70 -0.3 5,625,902 - CA265_RS22985 0.70 +0.5 5,625,902 - CA265_RS22985 0.70 -0.3 5,625,937 - CA265_RS22985 0.71 -0.3 5,625,952 - CA265_RS22985 0.72 +0.3 5,626,052 + CA265_RS22985 0.75 +0.1 5,626,053 - CA265_RS22985 0.75 -0.3 5,626,053 - CA265_RS22985 0.75 -1.6 5,626,098 - CA265_RS22985 0.77 +0.4 5,626,101 + CA265_RS22985 0.77 -0.6 5,626,129 - CA265_RS22985 0.78 -0.4 5,626,137 + CA265_RS22985 0.78 -0.4 5,626,170 + CA265_RS22985 0.79 +0.3 5,626,192 + CA265_RS22985 0.80 +0.7 5,626,192 + CA265_RS22985 0.80 +0.8 5,626,193 - CA265_RS22985 0.80 +0.3 5,626,264 + CA265_RS22985 0.82 -0.7 5,626,394 - CA265_RS22985 0.87 +0.6 5,626,397 + CA265_RS22985 0.87 -0.2 5,626,397 + CA265_RS22985 0.87 +1.7 5,626,532 + +0.0 5,626,587 - +0.2 5,626,594 - +1.2 5,626,600 + -0.1 5,626,712 + -0.1 5,626,734 - -0.0 5,626,744 - +0.7 5,626,753 - +0.3 5,626,758 + +0.3 5,626,769 - +1.6 5,626,800 + -0.5 5,626,801 - -0.9 5,626,824 + +0.3 5,626,829 + +1.0 5,626,840 + +1.5 5,626,840 + -0.1 5,626,909 + -0.3 5,626,972 - +1.1 5,626,973 + -1.1 5,626,988 + -0.7 5,627,005 + +0.3 5,627,012 - +0.6 5,627,052 - +0.5 5,627,135 + +0.1 5,627,138 - -0.5 5,627,209 - CA265_RS22990 0.15 +0.5 5,627,253 - CA265_RS22990 0.20 -0.5 5,627,263 - CA265_RS22990 0.21 -0.6 5,627,350 - CA265_RS22990 0.29 +0.2 5,627,357 + CA265_RS22990 0.30 +0.1 5,627,357 + CA265_RS22990 0.30 -0.3 5,627,359 + CA265_RS22990 0.30 -0.6 5,627,437 - CA265_RS22990 0.38 -0.7 5,627,447 + CA265_RS22990 0.39 -1.7 5,627,447 + CA265_RS22990 0.39 +0.2 5,627,447 + CA265_RS22990 0.39 -1.6 5,627,448 - CA265_RS22990 0.39 +0.3 5,627,448 - CA265_RS22990 0.39 +0.1 5,627,518 + CA265_RS22990 0.46 +0.1 5,627,542 - CA265_RS22990 0.48 -0.2 5,627,615 - CA265_RS22990 0.55 -0.6 5,627,615 - CA265_RS22990 0.55 -2.0 5,627,623 - CA265_RS22990 0.56 -0.5 5,627,624 + CA265_RS22990 0.56 +1.2 5,627,625 - CA265_RS22990 0.56 +0.1 5,627,625 - CA265_RS22990 0.56 +0.4 5,627,626 + CA265_RS22990 0.57 -0.6 5,627,626 + CA265_RS22990 0.57 -0.1 5,627,750 - CA265_RS22990 0.69 +1.1 5,627,750 - CA265_RS22990 0.69 -1.1
Or see this region's nucleotide sequence