Experiment: KB with Nalidixic 12.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3405 and Psyr_3406 are separated by 91 nucleotides Psyr_3406 and Psyr_3407 are separated by 728 nucleotides
Psyr_3405: Psyr_3405 - Abortive infection protein, at 4,065,180 to 4,065,977
_3405
Psyr_3406: Psyr_3406 - PAS, at 4,066,069 to 4,067,634
_3406
Psyr_3407: Psyr_3407 - conserved hypothetical protein, at 4,068,363 to 4,068,908
_3407
Position (kb)
4066
4067
4068 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4065.114 kb on + strand at 4065.115 kb on - strand at 4065.116 kb on + strand at 4065.116 kb on + strand at 4065.117 kb on - strand at 4065.175 kb on + strand at 4065.175 kb on + strand at 4065.175 kb on + strand at 4065.175 kb on + strand at 4065.175 kb on + strand at 4065.176 kb on - strand at 4065.176 kb on - strand at 4065.176 kb on - strand at 4065.176 kb on - strand at 4065.176 kb on - strand at 4065.176 kb on - strand at 4065.176 kb on - strand at 4065.176 kb on - strand at 4065.176 kb on - strand at 4065.176 kb on - strand at 4065.321 kb on + strand, within Psyr_3405 at 4065.321 kb on + strand, within Psyr_3405 at 4065.321 kb on + strand, within Psyr_3405 at 4065.322 kb on - strand, within Psyr_3405 at 4065.613 kb on + strand, within Psyr_3405 at 4065.613 kb on + strand, within Psyr_3405 at 4065.614 kb on - strand, within Psyr_3405 at 4065.614 kb on - strand, within Psyr_3405 at 4065.658 kb on + strand, within Psyr_3405 at 4065.718 kb on - strand, within Psyr_3405 at 4065.759 kb on + strand, within Psyr_3405 at 4065.759 kb on + strand, within Psyr_3405 at 4065.759 kb on + strand, within Psyr_3405 at 4065.759 kb on + strand, within Psyr_3405 at 4066.039 kb on + strand at 4066.039 kb on + strand at 4066.162 kb on + strand at 4066.162 kb on + strand at 4066.162 kb on + strand at 4066.162 kb on + strand at 4066.163 kb on - strand at 4066.163 kb on - strand at 4066.163 kb on - strand at 4066.163 kb on - strand at 4066.163 kb on - strand at 4066.163 kb on - strand at 4066.250 kb on + strand, within Psyr_3406 at 4066.250 kb on + strand, within Psyr_3406 at 4066.251 kb on - strand, within Psyr_3406 at 4066.345 kb on + strand, within Psyr_3406 at 4066.346 kb on - strand, within Psyr_3406 at 4066.360 kb on + strand, within Psyr_3406 at 4066.361 kb on - strand, within Psyr_3406 at 4066.456 kb on + strand, within Psyr_3406 at 4066.720 kb on + strand, within Psyr_3406 at 4066.720 kb on + strand, within Psyr_3406 at 4066.720 kb on + strand, within Psyr_3406 at 4066.720 kb on + strand, within Psyr_3406 at 4066.721 kb on - strand, within Psyr_3406 at 4066.721 kb on - strand, within Psyr_3406 at 4066.721 kb on - strand, within Psyr_3406 at 4066.721 kb on - strand, within Psyr_3406 at 4066.721 kb on - strand, within Psyr_3406 at 4066.721 kb on - strand, within Psyr_3406 at 4066.721 kb on - strand, within Psyr_3406 at 4067.246 kb on + strand, within Psyr_3406 at 4067.246 kb on + strand, within Psyr_3406 at 4067.247 kb on - strand, within Psyr_3406 at 4067.247 kb on - strand, within Psyr_3406 at 4067.247 kb on - strand, within Psyr_3406 at 4067.247 kb on - strand, within Psyr_3406 at 4067.606 kb on - strand at 4067.626 kb on - strand at 4067.741 kb on + strand at 4067.770 kb on - strand at 4067.803 kb on + strand at 4067.911 kb on + strand at 4068.023 kb on - strand at 4068.028 kb on + strand at 4068.042 kb on + strand at 4068.108 kb on - strand at 4068.237 kb on - strand at 4068.301 kb on + strand at 4068.301 kb on + strand at 4068.301 kb on + strand at 4068.301 kb on + strand at 4068.302 kb on - strand at 4068.302 kb on - strand at 4068.302 kb on - strand at 4068.302 kb on - strand at 4068.312 kb on + strand at 4068.361 kb on + strand at 4068.370 kb on + strand at 4068.410 kb on - strand at 4068.524 kb on + strand, within Psyr_3407 at 4068.605 kb on + strand, within Psyr_3407 at 4068.606 kb on - strand, within Psyr_3407 at 4068.614 kb on + strand, within Psyr_3407 at 4068.615 kb on - strand, within Psyr_3407
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Nalidixic 12.5 ug/ml remove 4,065,114 + -0.4 4,065,115 - -0.1 4,065,116 + -1.8 4,065,116 + +0.9 4,065,117 - +0.6 4,065,175 + +0.8 4,065,175 + -1.9 4,065,175 + +1.9 4,065,175 + -0.1 4,065,175 + +0.5 4,065,176 - +2.5 4,065,176 - -0.5 4,065,176 - +1.2 4,065,176 - -0.2 4,065,176 - -0.4 4,065,176 - -0.8 4,065,176 - -0.8 4,065,176 - -0.8 4,065,176 - +0.2 4,065,176 - -0.8 4,065,321 + Psyr_3405 0.18 +2.0 4,065,321 + Psyr_3405 0.18 +0.0 4,065,321 + Psyr_3405 0.18 +0.9 4,065,322 - Psyr_3405 0.18 -0.8 4,065,613 + Psyr_3405 0.54 -0.2 4,065,613 + Psyr_3405 0.54 +2.0 4,065,614 - Psyr_3405 0.54 -0.8 4,065,614 - Psyr_3405 0.54 -0.5 4,065,658 + Psyr_3405 0.60 -1.4 4,065,718 - Psyr_3405 0.67 +0.9 4,065,759 + Psyr_3405 0.73 -0.6 4,065,759 + Psyr_3405 0.73 -0.6 4,065,759 + Psyr_3405 0.73 -0.1 4,065,759 + Psyr_3405 0.73 +1.8 4,066,039 + +0.2 4,066,039 + +0.6 4,066,162 + +1.1 4,066,162 + -0.0 4,066,162 + -0.6 4,066,162 + +2.2 4,066,163 - -0.4 4,066,163 - +1.2 4,066,163 - +0.2 4,066,163 - +2.2 4,066,163 - -0.4 4,066,163 - +1.2 4,066,250 + Psyr_3406 0.12 +0.4 4,066,250 + Psyr_3406 0.12 +0.4 4,066,251 - Psyr_3406 0.12 -0.0 4,066,345 + Psyr_3406 0.18 -0.4 4,066,346 - Psyr_3406 0.18 +0.3 4,066,360 + Psyr_3406 0.19 -3.0 4,066,361 - Psyr_3406 0.19 -0.8 4,066,456 + Psyr_3406 0.25 +0.6 4,066,720 + Psyr_3406 0.42 -0.5 4,066,720 + Psyr_3406 0.42 +0.2 4,066,720 + Psyr_3406 0.42 -0.4 4,066,720 + Psyr_3406 0.42 -0.6 4,066,721 - Psyr_3406 0.42 -2.4 4,066,721 - Psyr_3406 0.42 +1.2 4,066,721 - Psyr_3406 0.42 +0.2 4,066,721 - Psyr_3406 0.42 +0.2 4,066,721 - Psyr_3406 0.42 +0.2 4,066,721 - Psyr_3406 0.42 +0.1 4,066,721 - Psyr_3406 0.42 +1.0 4,067,246 + Psyr_3406 0.75 +0.9 4,067,246 + Psyr_3406 0.75 -0.1 4,067,247 - Psyr_3406 0.75 -2.7 4,067,247 - Psyr_3406 0.75 -1.1 4,067,247 - Psyr_3406 0.75 -1.1 4,067,247 - Psyr_3406 0.75 -0.7 4,067,606 - +2.0 4,067,626 - -0.9 4,067,741 + -1.9 4,067,770 - -1.0 4,067,803 + -0.8 4,067,911 + +0.6 4,068,023 - +1.8 4,068,028 + -0.8 4,068,042 + -1.7 4,068,108 - -2.1 4,068,237 - +1.2 4,068,301 + +2.0 4,068,301 + +0.5 4,068,301 + +0.5 4,068,301 + -1.7 4,068,302 - -0.4 4,068,302 - -1.1 4,068,302 - +1.2 4,068,302 - -2.0 4,068,312 + +1.2 4,068,361 + -0.4 4,068,370 + -1.6 4,068,410 - +0.2 4,068,524 + Psyr_3407 0.29 +1.2 4,068,605 + Psyr_3407 0.44 +0.6 4,068,606 - Psyr_3407 0.45 -0.1 4,068,614 + Psyr_3407 0.46 +2.2 4,068,615 - Psyr_3407 0.46 +1.4
Or see this region's nucleotide sequence