Experiment: KB with Nalidixic 12.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3323 and Psyr_3324 are separated by 195 nucleotides Psyr_3324 and Psyr_3325 are separated by 11 nucleotides Psyr_3325 and Psyr_3326 overlap by 1 nucleotides
Psyr_3323: Psyr_3323 - conserved hypothetical protein, at 3,974,587 to 3,975,678
_3323
Psyr_3324: Psyr_3324 - transcriptional regulator, TetR family, at 3,975,874 to 3,976,488
_3324
Psyr_3325: Psyr_3325 - fumarylacetoacetate hydrolase, at 3,976,500 to 3,977,795
_3325
Psyr_3326: Psyr_3326 - homogentisate 1,2-dioxygenase, at 3,977,795 to 3,979,099
_3326
Position (kb)
3976
3977
3978 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6
7
8 at 3975.653 kb on + strand at 3975.729 kb on + strand at 3975.729 kb on + strand at 3975.729 kb on + strand at 3975.730 kb on - strand at 3975.730 kb on - strand at 3975.763 kb on + strand at 3975.763 kb on + strand at 3975.763 kb on + strand at 3975.763 kb on + strand at 3975.763 kb on + strand at 3975.763 kb on + strand at 3975.763 kb on + strand at 3975.764 kb on - strand at 3975.767 kb on + strand at 3975.767 kb on + strand at 3975.767 kb on + strand at 3975.767 kb on + strand at 3975.767 kb on + strand at 3975.767 kb on + strand at 3975.768 kb on - strand at 3975.768 kb on - strand at 3975.768 kb on - strand at 3975.825 kb on + strand at 3975.872 kb on + strand at 3975.873 kb on - strand at 3975.873 kb on - strand at 3975.873 kb on - strand at 3976.009 kb on - strand, within Psyr_3324 at 3976.015 kb on + strand, within Psyr_3324 at 3976.015 kb on + strand, within Psyr_3324 at 3976.015 kb on + strand, within Psyr_3324 at 3976.015 kb on + strand, within Psyr_3324 at 3976.015 kb on + strand, within Psyr_3324 at 3976.016 kb on - strand, within Psyr_3324 at 3976.016 kb on - strand, within Psyr_3324 at 3976.016 kb on - strand, within Psyr_3324 at 3976.016 kb on - strand, within Psyr_3324 at 3976.016 kb on - strand, within Psyr_3324 at 3976.016 kb on - strand, within Psyr_3324 at 3976.479 kb on - strand at 3976.700 kb on + strand, within Psyr_3325 at 3976.780 kb on + strand, within Psyr_3325 at 3976.780 kb on + strand, within Psyr_3325 at 3976.780 kb on + strand, within Psyr_3325 at 3976.780 kb on + strand, within Psyr_3325 at 3976.781 kb on - strand, within Psyr_3325 at 3976.781 kb on - strand, within Psyr_3325 at 3976.781 kb on - strand, within Psyr_3325 at 3976.781 kb on - strand, within Psyr_3325 at 3976.855 kb on - strand, within Psyr_3325 at 3976.855 kb on - strand, within Psyr_3325 at 3976.912 kb on + strand, within Psyr_3325 at 3976.912 kb on + strand, within Psyr_3325 at 3976.913 kb on - strand, within Psyr_3325 at 3977.047 kb on + strand, within Psyr_3325 at 3977.061 kb on + strand, within Psyr_3325 at 3977.061 kb on + strand, within Psyr_3325 at 3977.061 kb on + strand, within Psyr_3325 at 3977.062 kb on - strand, within Psyr_3325 at 3977.062 kb on - strand, within Psyr_3325 at 3977.062 kb on - strand, within Psyr_3325 at 3977.062 kb on - strand, within Psyr_3325 at 3977.062 kb on - strand, within Psyr_3325 at 3977.105 kb on + strand, within Psyr_3325 at 3977.105 kb on + strand, within Psyr_3325 at 3977.185 kb on + strand, within Psyr_3325 at 3977.317 kb on + strand, within Psyr_3325 at 3977.317 kb on + strand, within Psyr_3325 at 3977.317 kb on + strand, within Psyr_3325 at 3977.318 kb on - strand, within Psyr_3325 at 3977.318 kb on - strand, within Psyr_3325 at 3977.318 kb on - strand, within Psyr_3325 at 3977.318 kb on - strand, within Psyr_3325 at 3977.318 kb on - strand, within Psyr_3325 at 3977.318 kb on - strand, within Psyr_3325 at 3977.318 kb on - strand, within Psyr_3325 at 3977.318 kb on - strand, within Psyr_3325 at 3977.323 kb on + strand, within Psyr_3325 at 3977.323 kb on + strand, within Psyr_3325 at 3977.323 kb on + strand, within Psyr_3325 at 3977.323 kb on + strand, within Psyr_3325 at 3977.324 kb on - strand, within Psyr_3325 at 3977.324 kb on - strand, within Psyr_3325 at 3977.324 kb on - strand, within Psyr_3325 at 3977.392 kb on + strand, within Psyr_3325 at 3977.392 kb on + strand, within Psyr_3325 at 3977.392 kb on + strand, within Psyr_3325 at 3977.392 kb on + strand, within Psyr_3325 at 3977.393 kb on - strand, within Psyr_3325 at 3977.393 kb on - strand, within Psyr_3325 at 3977.393 kb on - strand, within Psyr_3325 at 3977.393 kb on - strand, within Psyr_3325 at 3977.393 kb on - strand, within Psyr_3325 at 3977.393 kb on - strand, within Psyr_3325 at 3977.847 kb on + strand at 3977.897 kb on + strand at 3977.897 kb on + strand at 3977.897 kb on + strand at 3977.897 kb on + strand at 3977.897 kb on + strand at 3977.898 kb on - strand at 3977.898 kb on - strand at 3977.981 kb on - strand, within Psyr_3326 at 3978.172 kb on + strand, within Psyr_3326 at 3978.191 kb on - strand, within Psyr_3326 at 3978.210 kb on - strand, within Psyr_3326 at 3978.210 kb on - strand, within Psyr_3326 at 3978.210 kb on - strand, within Psyr_3326 at 3978.240 kb on + strand, within Psyr_3326 at 3978.240 kb on + strand, within Psyr_3326 at 3978.240 kb on + strand, within Psyr_3326 at 3978.240 kb on + strand, within Psyr_3326 at 3978.241 kb on - strand, within Psyr_3326 at 3978.241 kb on - strand, within Psyr_3326 at 3978.250 kb on + strand, within Psyr_3326 at 3978.279 kb on + strand, within Psyr_3326 at 3978.279 kb on + strand, within Psyr_3326 at 3978.280 kb on - strand, within Psyr_3326 at 3978.280 kb on - strand, within Psyr_3326 at 3978.280 kb on - strand, within Psyr_3326 at 3978.280 kb on - strand, within Psyr_3326 at 3978.285 kb on + strand, within Psyr_3326 at 3978.285 kb on + strand, within Psyr_3326 at 3978.508 kb on - strand, within Psyr_3326 at 3978.696 kb on + strand, within Psyr_3326 at 3978.696 kb on + strand, within Psyr_3326 at 3978.697 kb on - strand, within Psyr_3326
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Nalidixic 12.5 ug/ml remove 3,975,653 + +0.6 3,975,729 + -2.5 3,975,729 + -0.2 3,975,729 + -0.5 3,975,730 - +2.3 3,975,730 - +1.0 3,975,763 + +0.4 3,975,763 + -0.3 3,975,763 + +0.7 3,975,763 + -0.4 3,975,763 + -1.7 3,975,763 + -1.1 3,975,763 + -0.1 3,975,764 - +1.2 3,975,767 + -0.2 3,975,767 + -1.8 3,975,767 + -2.5 3,975,767 + -1.2 3,975,767 + +0.2 3,975,767 + -2.2 3,975,768 - +0.5 3,975,768 - +0.4 3,975,768 - +1.2 3,975,825 + +1.0 3,975,872 + -0.8 3,975,873 - +7.1 3,975,873 - +7.2 3,975,873 - +6.8 3,976,009 - Psyr_3324 0.22 +2.5 3,976,015 + Psyr_3324 0.23 +6.6 3,976,015 + Psyr_3324 0.23 +8.5 3,976,015 + Psyr_3324 0.23 +6.1 3,976,015 + Psyr_3324 0.23 +6.5 3,976,015 + Psyr_3324 0.23 +7.3 3,976,016 - Psyr_3324 0.23 +7.1 3,976,016 - Psyr_3324 0.23 +6.2 3,976,016 - Psyr_3324 0.23 +6.5 3,976,016 - Psyr_3324 0.23 +6.6 3,976,016 - Psyr_3324 0.23 +6.2 3,976,016 - Psyr_3324 0.23 +7.6 3,976,479 - +5.3 3,976,700 + Psyr_3325 0.15 +0.4 3,976,780 + Psyr_3325 0.22 -0.6 3,976,780 + Psyr_3325 0.22 +0.4 3,976,780 + Psyr_3325 0.22 +1.2 3,976,780 + Psyr_3325 0.22 +0.4 3,976,781 - Psyr_3325 0.22 +1.1 3,976,781 - Psyr_3325 0.22 +0.9 3,976,781 - Psyr_3325 0.22 -0.2 3,976,781 - Psyr_3325 0.22 -1.6 3,976,855 - Psyr_3325 0.27 -0.1 3,976,855 - Psyr_3325 0.27 +1.7 3,976,912 + Psyr_3325 0.32 -0.2 3,976,912 + Psyr_3325 0.32 -0.4 3,976,913 - Psyr_3325 0.32 +0.3 3,977,047 + Psyr_3325 0.42 -1.6 3,977,061 + Psyr_3325 0.43 -1.5 3,977,061 + Psyr_3325 0.43 +0.9 3,977,061 + Psyr_3325 0.43 -2.2 3,977,062 - Psyr_3325 0.43 +1.4 3,977,062 - Psyr_3325 0.43 +0.5 3,977,062 - Psyr_3325 0.43 +0.7 3,977,062 - Psyr_3325 0.43 +1.0 3,977,062 - Psyr_3325 0.43 +2.2 3,977,105 + Psyr_3325 0.47 +0.2 3,977,105 + Psyr_3325 0.47 +0.6 3,977,185 + Psyr_3325 0.53 +0.1 3,977,317 + Psyr_3325 0.63 -0.8 3,977,317 + Psyr_3325 0.63 +1.2 3,977,317 + Psyr_3325 0.63 -1.9 3,977,318 - Psyr_3325 0.63 +0.6 3,977,318 - Psyr_3325 0.63 +1.2 3,977,318 - Psyr_3325 0.63 -0.4 3,977,318 - Psyr_3325 0.63 +0.2 3,977,318 - Psyr_3325 0.63 -1.2 3,977,318 - Psyr_3325 0.63 +0.2 3,977,318 - Psyr_3325 0.63 +0.2 3,977,318 - Psyr_3325 0.63 +0.8 3,977,323 + Psyr_3325 0.64 +0.4 3,977,323 + Psyr_3325 0.64 -0.5 3,977,323 + Psyr_3325 0.64 +0.1 3,977,323 + Psyr_3325 0.64 +0.8 3,977,324 - Psyr_3325 0.64 +1.8 3,977,324 - Psyr_3325 0.64 -0.7 3,977,324 - Psyr_3325 0.64 +1.2 3,977,392 + Psyr_3325 0.69 +0.1 3,977,392 + Psyr_3325 0.69 -1.1 3,977,392 + Psyr_3325 0.69 -0.2 3,977,392 + Psyr_3325 0.69 +0.8 3,977,393 - Psyr_3325 0.69 +0.2 3,977,393 - Psyr_3325 0.69 -2.2 3,977,393 - Psyr_3325 0.69 -0.1 3,977,393 - Psyr_3325 0.69 -0.4 3,977,393 - Psyr_3325 0.69 -1.2 3,977,393 - Psyr_3325 0.69 +0.2 3,977,847 + +0.5 3,977,897 + -0.4 3,977,897 + -0.7 3,977,897 + -2.3 3,977,897 + -0.2 3,977,897 + +0.0 3,977,898 - +0.2 3,977,898 - +0.1 3,977,981 - Psyr_3326 0.14 -0.8 3,978,172 + Psyr_3326 0.29 +0.4 3,978,191 - Psyr_3326 0.30 -0.4 3,978,210 - Psyr_3326 0.32 -0.8 3,978,210 - Psyr_3326 0.32 -0.5 3,978,210 - Psyr_3326 0.32 +2.2 3,978,240 + Psyr_3326 0.34 +0.6 3,978,240 + Psyr_3326 0.34 +1.8 3,978,240 + Psyr_3326 0.34 +1.2 3,978,240 + Psyr_3326 0.34 -1.1 3,978,241 - Psyr_3326 0.34 -0.6 3,978,241 - Psyr_3326 0.34 -0.8 3,978,250 + Psyr_3326 0.35 -0.6 3,978,279 + Psyr_3326 0.37 -1.4 3,978,279 + Psyr_3326 0.37 +0.1 3,978,280 - Psyr_3326 0.37 +1.0 3,978,280 - Psyr_3326 0.37 +0.5 3,978,280 - Psyr_3326 0.37 +1.2 3,978,280 - Psyr_3326 0.37 -2.1 3,978,285 + Psyr_3326 0.38 +0.1 3,978,285 + Psyr_3326 0.38 -0.4 3,978,508 - Psyr_3326 0.55 -1.7 3,978,696 + Psyr_3326 0.69 +0.2 3,978,696 + Psyr_3326 0.69 -0.8 3,978,697 - Psyr_3326 0.69 +0.9
Or see this region's nucleotide sequence