Experiment: KB with Nalidixic 12.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2668 and Psyr_2669 are separated by 65 nucleotides Psyr_2669 and Psyr_2670 are separated by 5 nucleotides Psyr_2670 and Psyr_2671 are separated by 41 nucleotides
Psyr_2668: Psyr_2668 - Helix-turn-helix, Fis-type, at 3,239,928 to 3,240,542
_2668
Psyr_2669: Psyr_2669 - Aminoglycoside phosphotransferase, at 3,240,608 to 3,241,411
_2669
Psyr_2670: Psyr_2670 - Aminoglycoside/hydroxyurea antibiotic resistance kinase, at 3,241,417 to 3,242,247
_2670
Psyr_2671: Psyr_2671 - transcriptional regulator, LysR family, at 3,242,289 to 3,243,230
_2671
Position (kb)
3241
3242
3243 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3240.536 kb on + strand at 3240.536 kb on + strand at 3240.536 kb on + strand at 3240.536 kb on + strand at 3240.537 kb on - strand at 3240.537 kb on - strand at 3240.537 kb on - strand at 3240.540 kb on + strand at 3240.540 kb on + strand at 3240.540 kb on + strand at 3240.540 kb on + strand at 3240.540 kb on + strand at 3240.620 kb on - strand at 3241.067 kb on - strand, within Psyr_2669 at 3241.139 kb on - strand, within Psyr_2669 at 3241.189 kb on + strand, within Psyr_2669 at 3241.247 kb on + strand at 3241.248 kb on - strand, within Psyr_2669 at 3241.329 kb on + strand, within Psyr_2669 at 3241.329 kb on + strand, within Psyr_2669 at 3241.330 kb on - strand, within Psyr_2669 at 3241.330 kb on - strand, within Psyr_2669 at 3241.338 kb on + strand at 3241.338 kb on + strand at 3241.338 kb on + strand at 3241.339 kb on - strand at 3241.339 kb on - strand at 3241.339 kb on - strand at 3241.339 kb on - strand at 3241.339 kb on - strand at 3241.379 kb on + strand at 3241.379 kb on + strand at 3241.379 kb on + strand at 3241.545 kb on + strand, within Psyr_2670 at 3241.545 kb on + strand, within Psyr_2670 at 3241.545 kb on + strand, within Psyr_2670 at 3241.546 kb on - strand, within Psyr_2670 at 3241.546 kb on - strand, within Psyr_2670 at 3241.546 kb on - strand at 3241.546 kb on - strand, within Psyr_2670 at 3241.546 kb on - strand, within Psyr_2670 at 3241.547 kb on + strand, within Psyr_2670 at 3241.547 kb on + strand, within Psyr_2670 at 3241.548 kb on - strand, within Psyr_2670 at 3241.548 kb on - strand, within Psyr_2670 at 3241.548 kb on - strand, within Psyr_2670 at 3241.548 kb on - strand, within Psyr_2670 at 3241.583 kb on - strand, within Psyr_2670 at 3241.589 kb on + strand, within Psyr_2670 at 3241.590 kb on - strand, within Psyr_2670 at 3241.654 kb on + strand, within Psyr_2670 at 3241.744 kb on + strand, within Psyr_2670 at 3241.744 kb on + strand, within Psyr_2670 at 3241.856 kb on - strand, within Psyr_2670 at 3241.856 kb on - strand, within Psyr_2670 at 3241.856 kb on - strand, within Psyr_2670 at 3241.872 kb on + strand, within Psyr_2670 at 3241.875 kb on - strand, within Psyr_2670 at 3241.875 kb on - strand, within Psyr_2670 at 3241.916 kb on + strand, within Psyr_2670 at 3241.973 kb on + strand, within Psyr_2670 at 3241.973 kb on + strand, within Psyr_2670 at 3241.973 kb on + strand, within Psyr_2670 at 3241.974 kb on - strand, within Psyr_2670 at 3242.019 kb on + strand, within Psyr_2670 at 3242.019 kb on + strand, within Psyr_2670 at 3242.019 kb on + strand, within Psyr_2670 at 3242.027 kb on - strand, within Psyr_2670 at 3242.201 kb on + strand at 3242.201 kb on + strand at 3242.201 kb on + strand at 3242.201 kb on + strand at 3242.201 kb on + strand at 3242.201 kb on + strand at 3242.201 kb on + strand at 3242.201 kb on + strand at 3242.201 kb on + strand at 3242.202 kb on - strand at 3242.202 kb on - strand at 3242.202 kb on - strand at 3242.202 kb on - strand at 3242.202 kb on - strand at 3242.202 kb on - strand at 3242.202 kb on - strand at 3242.243 kb on - strand at 3242.243 kb on - strand at 3242.245 kb on + strand at 3242.245 kb on + strand at 3242.245 kb on + strand at 3242.245 kb on + strand at 3242.245 kb on + strand at 3242.245 kb on + strand at 3242.245 kb on + strand at 3242.245 kb on + strand at 3242.246 kb on - strand at 3242.246 kb on - strand at 3242.246 kb on - strand at 3242.246 kb on - strand at 3242.246 kb on - strand at 3242.246 kb on - strand at 3242.246 kb on - strand at 3242.246 kb on - strand at 3242.246 kb on - strand at 3242.246 kb on - strand at 3242.246 kb on - strand at 3242.246 kb on - strand at 3242.250 kb on - strand at 3242.267 kb on - strand at 3242.373 kb on - strand at 3242.375 kb on + strand at 3242.376 kb on - strand at 3242.465 kb on + strand, within Psyr_2671 at 3242.509 kb on + strand, within Psyr_2671 at 3242.509 kb on + strand, within Psyr_2671 at 3242.509 kb on + strand, within Psyr_2671 at 3242.509 kb on + strand, within Psyr_2671 at 3242.509 kb on + strand, within Psyr_2671 at 3242.509 kb on + strand, within Psyr_2671 at 3242.509 kb on + strand, within Psyr_2671 at 3242.510 kb on - strand, within Psyr_2671 at 3242.510 kb on - strand, within Psyr_2671 at 3242.510 kb on - strand, within Psyr_2671 at 3242.510 kb on - strand, within Psyr_2671 at 3242.510 kb on - strand, within Psyr_2671 at 3242.510 kb on - strand, within Psyr_2671 at 3242.510 kb on - strand, within Psyr_2671 at 3242.510 kb on - strand, within Psyr_2671 at 3242.510 kb on - strand, within Psyr_2671 at 3242.532 kb on + strand, within Psyr_2671 at 3242.533 kb on - strand, within Psyr_2671 at 3242.597 kb on + strand, within Psyr_2671 at 3242.619 kb on + strand, within Psyr_2671 at 3242.620 kb on - strand, within Psyr_2671 at 3242.620 kb on - strand, within Psyr_2671 at 3242.620 kb on - strand, within Psyr_2671 at 3242.620 kb on - strand, within Psyr_2671 at 3242.620 kb on - strand, within Psyr_2671 at 3242.620 kb on - strand, within Psyr_2671 at 3242.737 kb on - strand, within Psyr_2671 at 3242.737 kb on - strand, within Psyr_2671 at 3242.855 kb on + strand, within Psyr_2671 at 3242.855 kb on + strand, within Psyr_2671 at 3242.862 kb on + strand, within Psyr_2671 at 3243.102 kb on + strand, within Psyr_2671 at 3243.102 kb on + strand, within Psyr_2671 at 3243.102 kb on + strand, within Psyr_2671 at 3243.103 kb on - strand, within Psyr_2671 at 3243.211 kb on + strand at 3243.212 kb on - strand at 3243.243 kb on - strand at 3243.243 kb on - strand at 3243.243 kb on - strand at 3243.245 kb on + strand at 3243.245 kb on + strand at 3243.245 kb on + strand at 3243.246 kb on - strand at 3243.246 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Nalidixic 12.5 ug/ml remove 3,240,536 + -0.8 3,240,536 + +0.4 3,240,536 + -0.2 3,240,536 + +0.3 3,240,537 - +0.8 3,240,537 - -0.1 3,240,537 - -0.9 3,240,540 + +0.4 3,240,540 + -0.4 3,240,540 + -0.6 3,240,540 + +0.2 3,240,540 + +1.0 3,240,620 - +1.2 3,241,067 - Psyr_2669 0.57 -0.6 3,241,139 - Psyr_2669 0.66 +0.3 3,241,189 + Psyr_2669 0.72 -1.4 3,241,247 + -0.6 3,241,248 - Psyr_2669 0.80 -0.0 3,241,329 + Psyr_2669 0.90 -0.8 3,241,329 + Psyr_2669 0.90 +0.2 3,241,330 - Psyr_2669 0.90 +0.8 3,241,330 - Psyr_2669 0.90 -1.6 3,241,338 + -0.4 3,241,338 + -0.2 3,241,338 + +0.8 3,241,339 - +1.2 3,241,339 - -1.4 3,241,339 - +0.8 3,241,339 - -1.4 3,241,339 - +0.3 3,241,379 + +0.3 3,241,379 + -0.9 3,241,379 + -0.6 3,241,545 + Psyr_2670 0.15 -1.2 3,241,545 + Psyr_2670 0.15 -0.7 3,241,545 + Psyr_2670 0.15 +1.3 3,241,546 - Psyr_2670 0.16 -2.2 3,241,546 - Psyr_2670 0.16 +0.3 3,241,546 - -0.2 3,241,546 - Psyr_2670 0.16 -1.4 3,241,546 - Psyr_2670 0.16 +1.8 3,241,547 + Psyr_2670 0.16 -0.6 3,241,547 + Psyr_2670 0.16 -1.0 3,241,548 - Psyr_2670 0.16 -0.2 3,241,548 - Psyr_2670 0.16 -1.9 3,241,548 - Psyr_2670 0.16 -0.4 3,241,548 - Psyr_2670 0.16 +0.5 3,241,583 - Psyr_2670 0.20 +0.2 3,241,589 + Psyr_2670 0.21 +1.0 3,241,590 - Psyr_2670 0.21 -1.0 3,241,654 + Psyr_2670 0.29 -1.4 3,241,744 + Psyr_2670 0.39 -0.8 3,241,744 + Psyr_2670 0.39 -1.6 3,241,856 - Psyr_2670 0.53 -2.3 3,241,856 - Psyr_2670 0.53 -1.0 3,241,856 - Psyr_2670 0.53 +1.0 3,241,872 + Psyr_2670 0.55 +0.8 3,241,875 - Psyr_2670 0.55 -0.9 3,241,875 - Psyr_2670 0.55 -0.4 3,241,916 + Psyr_2670 0.60 +1.3 3,241,973 + Psyr_2670 0.67 -0.4 3,241,973 + Psyr_2670 0.67 -1.6 3,241,973 + Psyr_2670 0.67 -1.5 3,241,974 - Psyr_2670 0.67 -2.1 3,242,019 + Psyr_2670 0.72 +0.3 3,242,019 + Psyr_2670 0.72 +0.1 3,242,019 + Psyr_2670 0.72 -1.8 3,242,027 - Psyr_2670 0.73 -1.2 3,242,201 + -1.8 3,242,201 + +0.2 3,242,201 + +0.8 3,242,201 + -1.6 3,242,201 + -1.6 3,242,201 + -2.4 3,242,201 + -1.2 3,242,201 + -0.9 3,242,201 + -0.6 3,242,202 - -1.4 3,242,202 - -1.2 3,242,202 - -0.8 3,242,202 - -1.7 3,242,202 - +0.2 3,242,202 - -0.1 3,242,202 - -1.7 3,242,243 - +0.2 3,242,243 - -1.2 3,242,245 + -0.8 3,242,245 + -1.0 3,242,245 + -0.2 3,242,245 + -1.6 3,242,245 + +1.8 3,242,245 + +0.2 3,242,245 + -2.2 3,242,245 + -0.8 3,242,246 - +0.2 3,242,246 - -0.8 3,242,246 - -0.2 3,242,246 - +0.2 3,242,246 - -2.2 3,242,246 - -1.1 3,242,246 - +0.4 3,242,246 - +0.2 3,242,246 - -1.0 3,242,246 - -0.7 3,242,246 - +1.2 3,242,246 - -0.0 3,242,250 - -0.7 3,242,267 - +1.8 3,242,373 - +1.0 3,242,375 + -2.0 3,242,376 - +0.4 3,242,465 + Psyr_2671 0.19 -0.6 3,242,509 + Psyr_2671 0.23 +0.5 3,242,509 + Psyr_2671 0.23 +0.7 3,242,509 + Psyr_2671 0.23 -0.2 3,242,509 + Psyr_2671 0.23 +0.3 3,242,509 + Psyr_2671 0.23 +0.8 3,242,509 + Psyr_2671 0.23 +0.3 3,242,509 + Psyr_2671 0.23 -0.2 3,242,510 - Psyr_2671 0.23 +0.2 3,242,510 - Psyr_2671 0.23 +0.8 3,242,510 - Psyr_2671 0.23 +1.0 3,242,510 - Psyr_2671 0.23 -0.1 3,242,510 - Psyr_2671 0.23 -0.4 3,242,510 - Psyr_2671 0.23 +0.8 3,242,510 - Psyr_2671 0.23 +0.1 3,242,510 - Psyr_2671 0.23 -1.9 3,242,510 - Psyr_2671 0.23 -0.4 3,242,532 + Psyr_2671 0.26 -0.8 3,242,533 - Psyr_2671 0.26 -0.2 3,242,597 + Psyr_2671 0.33 -0.5 3,242,619 + Psyr_2671 0.35 -0.7 3,242,620 - Psyr_2671 0.35 +1.8 3,242,620 - Psyr_2671 0.35 -1.0 3,242,620 - Psyr_2671 0.35 +0.5 3,242,620 - Psyr_2671 0.35 +1.8 3,242,620 - Psyr_2671 0.35 +0.2 3,242,620 - Psyr_2671 0.35 +0.3 3,242,737 - Psyr_2671 0.48 -0.7 3,242,737 - Psyr_2671 0.48 -0.0 3,242,855 + Psyr_2671 0.60 -2.2 3,242,855 + Psyr_2671 0.60 -1.0 3,242,862 + Psyr_2671 0.61 -0.2 3,243,102 + Psyr_2671 0.86 -0.9 3,243,102 + Psyr_2671 0.86 -1.6 3,243,102 + Psyr_2671 0.86 -2.5 3,243,103 - Psyr_2671 0.86 +0.8 3,243,211 + -1.4 3,243,212 - -0.2 3,243,243 - -0.7 3,243,243 - -1.7 3,243,243 - +0.2 3,243,245 + -0.6 3,243,245 + -0.7 3,243,245 + -1.0 3,243,246 - +0.8 3,243,246 - +0.4
Or see this region's nucleotide sequence