Experiment: KB with Nalidixic 12.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2554 and Psyr_2555 are separated by 4 nucleotides Psyr_2555 and Psyr_2556 are separated by 2 nucleotides Psyr_2556 and Psyr_2557 overlap by 11 nucleotides
Psyr_2554: Psyr_2554 - Fatty acid desaturase, at 2,967,617 to 2,968,633
_2554
Psyr_2555: Psyr_2555 - (dCTP deaminase), deoxycytidine triphosphate deaminase, at 2,968,638 to 2,969,150
_2555
Psyr_2556: Psyr_2556 - dCTP deaminase, at 2,969,153 to 2,969,827
_2556
Psyr_2557: Psyr_2557 - Phosphoenolpyruvate synthase/pyruvate phosphate dikinase-like protein, at 2,969,817 to 2,971,712
_2557
Position (kb)
2969
2970 Strain fitness (log2 ratio)
-1
0
1
2 at 2968.214 kb on + strand, within Psyr_2554 at 2968.214 kb on + strand, within Psyr_2554 at 2968.215 kb on - strand, within Psyr_2554 at 2968.215 kb on - strand, within Psyr_2554 at 2968.215 kb on - strand, within Psyr_2554 at 2968.215 kb on - strand, within Psyr_2554 at 2968.272 kb on - strand, within Psyr_2554 at 2968.470 kb on - strand, within Psyr_2554 at 2968.545 kb on - strand at 2968.580 kb on + strand at 2968.580 kb on + strand at 2968.580 kb on + strand at 2968.581 kb on - strand at 2968.660 kb on + strand at 2968.817 kb on + strand, within Psyr_2555 at 2968.818 kb on - strand, within Psyr_2555 at 2968.858 kb on - strand, within Psyr_2555 at 2969.104 kb on - strand at 2969.157 kb on + strand at 2969.247 kb on - strand, within Psyr_2556 at 2969.247 kb on - strand, within Psyr_2556 at 2969.372 kb on + strand, within Psyr_2556 at 2969.373 kb on - strand, within Psyr_2556 at 2969.388 kb on + strand, within Psyr_2556 at 2969.389 kb on - strand, within Psyr_2556 at 2969.419 kb on + strand, within Psyr_2556 at 2969.419 kb on + strand, within Psyr_2556 at 2969.420 kb on - strand, within Psyr_2556 at 2969.420 kb on - strand, within Psyr_2556 at 2969.423 kb on + strand, within Psyr_2556 at 2969.606 kb on + strand, within Psyr_2556 at 2969.721 kb on + strand, within Psyr_2556 at 2969.829 kb on + strand at 2969.829 kb on + strand at 2969.829 kb on + strand at 2969.830 kb on - strand at 2970.672 kb on + strand, within Psyr_2557 at 2970.729 kb on + strand, within Psyr_2557
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Nalidixic 12.5 ug/ml remove 2,968,214 + Psyr_2554 0.59 +1.4 2,968,214 + Psyr_2554 0.59 +0.2 2,968,215 - Psyr_2554 0.59 -0.6 2,968,215 - Psyr_2554 0.59 +0.6 2,968,215 - Psyr_2554 0.59 +2.1 2,968,215 - Psyr_2554 0.59 +1.2 2,968,272 - Psyr_2554 0.64 -0.2 2,968,470 - Psyr_2554 0.84 +1.3 2,968,545 - +0.2 2,968,580 + +0.4 2,968,580 + +0.3 2,968,580 + +1.8 2,968,581 - -0.4 2,968,660 + +1.2 2,968,817 + Psyr_2555 0.35 -0.8 2,968,818 - Psyr_2555 0.35 +1.2 2,968,858 - Psyr_2555 0.43 -1.4 2,969,104 - -0.7 2,969,157 + -0.2 2,969,247 - Psyr_2556 0.14 +2.0 2,969,247 - Psyr_2556 0.14 +1.8 2,969,372 + Psyr_2556 0.32 +0.9 2,969,373 - Psyr_2556 0.33 +0.8 2,969,388 + Psyr_2556 0.35 +1.2 2,969,389 - Psyr_2556 0.35 +0.2 2,969,419 + Psyr_2556 0.39 +0.2 2,969,419 + Psyr_2556 0.39 +0.3 2,969,420 - Psyr_2556 0.40 -0.2 2,969,420 - Psyr_2556 0.40 -0.6 2,969,423 + Psyr_2556 0.40 -0.4 2,969,606 + Psyr_2556 0.67 -1.4 2,969,721 + Psyr_2556 0.84 -0.2 2,969,829 + -1.4 2,969,829 + +0.1 2,969,829 + +0.2 2,969,830 - -0.6 2,970,672 + Psyr_2557 0.45 -1.4 2,970,729 + Psyr_2557 0.48 +0.2
Or see this region's nucleotide sequence