Experiment: KB with Nalidixic 12.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2492 and Psyr_2493 are separated by 261 nucleotides Psyr_2493 and Psyr_2494 are separated by 125 nucleotides
Psyr_2492: Psyr_2492 - Outer membrane autotransporter barrel, at 2,899,921 to 2,902,665
_2492
Psyr_2493: Psyr_2493 - Major facilitator superfamily, at 2,902,927 to 2,904,150
_2493
Psyr_2494: Psyr_2494 - conserved hypothetical protein, at 2,904,276 to 2,905,409
_2494
Position (kb)
2902
2903
2904
2905 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2902.110 kb on + strand, within Psyr_2492 at 2902.132 kb on + strand, within Psyr_2492 at 2902.132 kb on + strand, within Psyr_2492 at 2902.133 kb on - strand, within Psyr_2492 at 2902.133 kb on - strand, within Psyr_2492 at 2902.243 kb on - strand, within Psyr_2492 at 2902.444 kb on - strand at 2902.480 kb on + strand at 2902.480 kb on + strand at 2902.480 kb on + strand at 2902.481 kb on - strand at 2902.486 kb on + strand at 2902.486 kb on + strand at 2902.486 kb on + strand at 2902.486 kb on + strand at 2902.486 kb on + strand at 2902.487 kb on - strand at 2902.487 kb on - strand at 2902.487 kb on - strand at 2902.487 kb on - strand at 2902.487 kb on - strand at 2902.609 kb on + strand at 2902.613 kb on - strand at 2902.731 kb on - strand at 2902.864 kb on - strand at 2902.874 kb on + strand at 2902.874 kb on + strand at 2902.883 kb on + strand at 2902.884 kb on - strand at 2902.899 kb on - strand at 2903.000 kb on + strand at 2903.090 kb on + strand, within Psyr_2493 at 2903.090 kb on + strand, within Psyr_2493 at 2903.137 kb on - strand, within Psyr_2493 at 2903.143 kb on + strand, within Psyr_2493 at 2903.390 kb on + strand, within Psyr_2493 at 2903.390 kb on + strand, within Psyr_2493 at 2903.645 kb on + strand, within Psyr_2493 at 2903.645 kb on + strand, within Psyr_2493 at 2903.890 kb on - strand, within Psyr_2493 at 2903.890 kb on - strand, within Psyr_2493 at 2903.937 kb on - strand, within Psyr_2493 at 2903.937 kb on - strand, within Psyr_2493 at 2904.027 kb on + strand, within Psyr_2493 at 2904.050 kb on + strand at 2904.167 kb on - strand at 2904.188 kb on - strand at 2904.228 kb on + strand at 2904.259 kb on + strand at 2904.263 kb on - strand at 2904.351 kb on + strand at 2904.351 kb on + strand at 2904.351 kb on + strand at 2904.352 kb on - strand at 2904.352 kb on - strand at 2904.352 kb on - strand at 2904.352 kb on - strand at 2904.352 kb on - strand at 2904.352 kb on - strand at 2904.352 kb on - strand at 2904.353 kb on + strand at 2904.354 kb on - strand at 2904.598 kb on - strand, within Psyr_2494 at 2904.643 kb on - strand, within Psyr_2494 at 2904.643 kb on - strand, within Psyr_2494 at 2904.711 kb on + strand, within Psyr_2494 at 2904.711 kb on + strand, within Psyr_2494 at 2904.711 kb on + strand, within Psyr_2494 at 2904.711 kb on + strand, within Psyr_2494 at 2904.712 kb on - strand, within Psyr_2494 at 2904.712 kb on - strand, within Psyr_2494 at 2904.852 kb on + strand, within Psyr_2494 at 2904.852 kb on + strand, within Psyr_2494 at 2904.852 kb on + strand, within Psyr_2494 at 2904.853 kb on - strand, within Psyr_2494 at 2904.853 kb on - strand, within Psyr_2494 at 2904.853 kb on - strand, within Psyr_2494 at 2904.918 kb on + strand, within Psyr_2494 at 2904.918 kb on + strand, within Psyr_2494 at 2904.918 kb on + strand, within Psyr_2494 at 2904.918 kb on + strand, within Psyr_2494 at 2904.919 kb on - strand, within Psyr_2494
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Nalidixic 12.5 ug/ml remove 2,902,110 + Psyr_2492 0.80 +0.5 2,902,132 + Psyr_2492 0.81 -1.2 2,902,132 + Psyr_2492 0.81 -2.3 2,902,133 - Psyr_2492 0.81 -3.1 2,902,133 - Psyr_2492 0.81 +0.1 2,902,243 - Psyr_2492 0.85 -0.0 2,902,444 - -0.2 2,902,480 + +0.7 2,902,480 + +1.6 2,902,480 + +1.2 2,902,481 - +0.4 2,902,486 + -0.5 2,902,486 + -0.7 2,902,486 + +2.4 2,902,486 + +0.6 2,902,486 + -0.7 2,902,487 - -0.1 2,902,487 - +0.6 2,902,487 - -0.2 2,902,487 - +1.6 2,902,487 - +0.4 2,902,609 + +0.4 2,902,613 - +0.2 2,902,731 - -1.3 2,902,864 - -1.3 2,902,874 + -1.4 2,902,874 + -1.7 2,902,883 + -0.4 2,902,884 - -1.1 2,902,899 - +2.3 2,903,000 + +0.9 2,903,090 + Psyr_2493 0.13 -0.3 2,903,090 + Psyr_2493 0.13 +0.1 2,903,137 - Psyr_2493 0.17 -0.2 2,903,143 + Psyr_2493 0.18 -0.9 2,903,390 + Psyr_2493 0.38 -0.0 2,903,390 + Psyr_2493 0.38 -0.8 2,903,645 + Psyr_2493 0.59 -1.4 2,903,645 + Psyr_2493 0.59 +0.2 2,903,890 - Psyr_2493 0.79 +1.1 2,903,890 - Psyr_2493 0.79 -0.1 2,903,937 - Psyr_2493 0.83 -0.6 2,903,937 - Psyr_2493 0.83 +0.2 2,904,027 + Psyr_2493 0.90 -1.3 2,904,050 + -1.7 2,904,167 - -0.7 2,904,188 - -0.7 2,904,228 + -0.5 2,904,259 + +1.2 2,904,263 - -1.8 2,904,351 + -1.2 2,904,351 + +1.3 2,904,351 + +0.4 2,904,352 - -2.0 2,904,352 - -1.2 2,904,352 - -0.9 2,904,352 - +0.7 2,904,352 - -1.9 2,904,352 - +0.2 2,904,352 - -2.0 2,904,353 + -1.2 2,904,354 - -0.3 2,904,598 - Psyr_2494 0.28 -1.7 2,904,643 - Psyr_2494 0.32 -0.7 2,904,643 - Psyr_2494 0.32 +1.4 2,904,711 + Psyr_2494 0.38 +0.2 2,904,711 + Psyr_2494 0.38 -0.3 2,904,711 + Psyr_2494 0.38 +1.1 2,904,711 + Psyr_2494 0.38 +1.0 2,904,712 - Psyr_2494 0.38 -0.3 2,904,712 - Psyr_2494 0.38 -0.3 2,904,852 + Psyr_2494 0.51 -1.6 2,904,852 + Psyr_2494 0.51 +0.7 2,904,852 + Psyr_2494 0.51 +0.3 2,904,853 - Psyr_2494 0.51 -0.2 2,904,853 - Psyr_2494 0.51 -0.9 2,904,853 - Psyr_2494 0.51 +0.4 2,904,918 + Psyr_2494 0.57 +2.1 2,904,918 + Psyr_2494 0.57 +0.3 2,904,918 + Psyr_2494 0.57 +1.3 2,904,918 + Psyr_2494 0.57 -0.3 2,904,919 - Psyr_2494 0.57 +0.5
Or see this region's nucleotide sequence