Experiment: KB with Nalidixic 12.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2432 and Psyr_2433 are separated by 168 nucleotides Psyr_2433 and Psyr_2434 are separated by 84 nucleotides Psyr_2434 and Psyr_2435 are separated by 22 nucleotides
Psyr_2432: Psyr_2432 - diguanylate cyclase/phosphodiesterase, at 2,814,290 to 2,817,199
_2432
Psyr_2433: Psyr_2433 - conserved hypothetical protein, at 2,817,368 to 2,817,922
_2433
Psyr_2434: Psyr_2434 - Carbohydrate kinase, PfkB, at 2,818,007 to 2,818,945
_2434
Psyr_2435: Psyr_2435 - Xylulokinase, at 2,818,968 to 2,820,449
_2435
Position (kb)
2818
2819 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2817.137 kb on + strand at 2817.137 kb on + strand at 2817.137 kb on + strand at 2817.137 kb on + strand at 2817.138 kb on - strand at 2817.138 kb on - strand at 2817.373 kb on + strand at 2817.373 kb on + strand at 2817.374 kb on - strand at 2817.557 kb on + strand, within Psyr_2433 at 2817.557 kb on + strand, within Psyr_2433 at 2817.558 kb on - strand, within Psyr_2433 at 2817.558 kb on - strand, within Psyr_2433 at 2817.607 kb on + strand, within Psyr_2433 at 2817.608 kb on - strand, within Psyr_2433 at 2817.705 kb on + strand, within Psyr_2433 at 2817.788 kb on + strand, within Psyr_2433 at 2817.789 kb on - strand, within Psyr_2433 at 2817.789 kb on - strand, within Psyr_2433 at 2817.789 kb on - strand, within Psyr_2433 at 2817.789 kb on - strand, within Psyr_2433 at 2817.985 kb on + strand at 2818.184 kb on - strand at 2818.303 kb on + strand, within Psyr_2434 at 2818.304 kb on - strand, within Psyr_2434 at 2818.304 kb on - strand, within Psyr_2434 at 2818.647 kb on + strand, within Psyr_2434 at 2818.647 kb on + strand, within Psyr_2434 at 2818.647 kb on + strand, within Psyr_2434 at 2818.648 kb on - strand, within Psyr_2434 at 2818.648 kb on - strand, within Psyr_2434 at 2818.648 kb on - strand, within Psyr_2434 at 2818.771 kb on - strand, within Psyr_2434 at 2818.945 kb on + strand at 2818.945 kb on + strand at 2818.945 kb on + strand at 2818.945 kb on + strand at 2818.945 kb on + strand at 2818.945 kb on + strand at 2818.946 kb on - strand at 2818.946 kb on - strand at 2818.994 kb on - strand at 2819.002 kb on + strand at 2819.318 kb on + strand, within Psyr_2435 at 2819.641 kb on + strand, within Psyr_2435 at 2819.641 kb on + strand, within Psyr_2435 at 2819.641 kb on + strand, within Psyr_2435 at 2819.642 kb on - strand, within Psyr_2435 at 2819.691 kb on - strand, within Psyr_2435
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Nalidixic 12.5 ug/ml remove 2,817,137 + -0.7 2,817,137 + +0.6 2,817,137 + +2.0 2,817,137 + +0.4 2,817,138 - +0.4 2,817,138 - +0.2 2,817,373 + +0.5 2,817,373 + +0.0 2,817,374 - -0.5 2,817,557 + Psyr_2433 0.34 -2.0 2,817,557 + Psyr_2433 0.34 +1.5 2,817,558 - Psyr_2433 0.34 +0.3 2,817,558 - Psyr_2433 0.34 -1.1 2,817,607 + Psyr_2433 0.43 +0.6 2,817,608 - Psyr_2433 0.43 -0.4 2,817,705 + Psyr_2433 0.61 -1.5 2,817,788 + Psyr_2433 0.76 +1.6 2,817,789 - Psyr_2433 0.76 -0.4 2,817,789 - Psyr_2433 0.76 -2.5 2,817,789 - Psyr_2433 0.76 -2.0 2,817,789 - Psyr_2433 0.76 -1.4 2,817,985 + +2.6 2,818,184 - +1.6 2,818,303 + Psyr_2434 0.32 +1.6 2,818,304 - Psyr_2434 0.32 -0.4 2,818,304 - Psyr_2434 0.32 +3.2 2,818,647 + Psyr_2434 0.68 -1.0 2,818,647 + Psyr_2434 0.68 +1.6 2,818,647 + Psyr_2434 0.68 +0.6 2,818,648 - Psyr_2434 0.68 +0.6 2,818,648 - Psyr_2434 0.68 +1.6 2,818,648 - Psyr_2434 0.68 +1.9 2,818,771 - Psyr_2434 0.81 -1.8 2,818,945 + -0.1 2,818,945 + -0.4 2,818,945 + -1.6 2,818,945 + +0.9 2,818,945 + +0.5 2,818,945 + -2.2 2,818,946 - -1.2 2,818,946 - -0.5 2,818,994 - +0.4 2,819,002 + +0.7 2,819,318 + Psyr_2435 0.24 +1.6 2,819,641 + Psyr_2435 0.45 +1.0 2,819,641 + Psyr_2435 0.45 +0.0 2,819,641 + Psyr_2435 0.45 -1.8 2,819,642 - Psyr_2435 0.45 -0.3 2,819,691 - Psyr_2435 0.49 +0.0
Or see this region's nucleotide sequence