Experiment: KB with Nalidixic 12.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2298 and Psyr_2299 are separated by 56 nucleotides Psyr_2299 and Psyr_2300 are separated by 53 nucleotides
Psyr_2298: Psyr_2298 - Glycoside hydrolase, family 15, at 2,670,080 to 2,671,906
_2298
Psyr_2299: Psyr_2299 - hypothetical protein, at 2,671,963 to 2,673,390
_2299
Psyr_2300: Psyr_2300 - Conserved hypothetical protein 374, at 2,673,444 to 2,674,412
_2300
Position (kb)
2671
2672
2673
2674 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2671.101 kb on + strand, within Psyr_2298 at 2671.102 kb on - strand, within Psyr_2298 at 2671.106 kb on - strand, within Psyr_2298 at 2671.122 kb on - strand, within Psyr_2298 at 2671.122 kb on - strand, within Psyr_2298 at 2671.122 kb on - strand, within Psyr_2298 at 2671.145 kb on + strand, within Psyr_2298 at 2671.170 kb on - strand, within Psyr_2298 at 2671.170 kb on - strand, within Psyr_2298 at 2671.185 kb on - strand, within Psyr_2298 at 2671.238 kb on + strand, within Psyr_2298 at 2671.238 kb on + strand, within Psyr_2298 at 2671.239 kb on - strand, within Psyr_2298 at 2671.522 kb on - strand, within Psyr_2298 at 2671.522 kb on - strand, within Psyr_2298 at 2671.772 kb on + strand at 2671.772 kb on + strand at 2671.773 kb on - strand at 2671.813 kb on + strand at 2672.033 kb on + strand at 2672.051 kb on + strand at 2672.210 kb on + strand, within Psyr_2299 at 2672.249 kb on + strand, within Psyr_2299 at 2672.265 kb on - strand, within Psyr_2299 at 2672.339 kb on + strand, within Psyr_2299 at 2672.340 kb on - strand, within Psyr_2299 at 2672.340 kb on - strand, within Psyr_2299 at 2672.419 kb on - strand, within Psyr_2299 at 2672.540 kb on - strand, within Psyr_2299 at 2672.711 kb on + strand, within Psyr_2299 at 2672.712 kb on - strand, within Psyr_2299 at 2672.712 kb on - strand, within Psyr_2299 at 2672.816 kb on - strand, within Psyr_2299 at 2672.816 kb on - strand, within Psyr_2299 at 2672.849 kb on + strand, within Psyr_2299 at 2672.849 kb on + strand, within Psyr_2299 at 2672.909 kb on - strand, within Psyr_2299 at 2672.963 kb on + strand, within Psyr_2299 at 2672.972 kb on + strand, within Psyr_2299 at 2673.005 kb on + strand, within Psyr_2299 at 2673.005 kb on + strand, within Psyr_2299 at 2673.005 kb on + strand, within Psyr_2299 at 2673.196 kb on - strand, within Psyr_2299 at 2673.361 kb on - strand at 2673.479 kb on + strand at 2673.537 kb on + strand at 2673.670 kb on + strand, within Psyr_2300 at 2673.674 kb on + strand, within Psyr_2300 at 2673.674 kb on + strand, within Psyr_2300 at 2673.678 kb on + strand, within Psyr_2300 at 2673.678 kb on + strand, within Psyr_2300 at 2673.849 kb on - strand, within Psyr_2300 at 2674.150 kb on + strand, within Psyr_2300 at 2674.150 kb on + strand, within Psyr_2300 at 2674.201 kb on + strand, within Psyr_2300 at 2674.201 kb on + strand, within Psyr_2300 at 2674.337 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Nalidixic 12.5 ug/ml remove 2,671,101 + Psyr_2298 0.56 -0.4 2,671,102 - Psyr_2298 0.56 +1.1 2,671,106 - Psyr_2298 0.56 +0.7 2,671,122 - Psyr_2298 0.57 +2.9 2,671,122 - Psyr_2298 0.57 -0.5 2,671,122 - Psyr_2298 0.57 +0.2 2,671,145 + Psyr_2298 0.58 +0.4 2,671,170 - Psyr_2298 0.60 -0.8 2,671,170 - Psyr_2298 0.60 -0.2 2,671,185 - Psyr_2298 0.60 +0.6 2,671,238 + Psyr_2298 0.63 -0.3 2,671,238 + Psyr_2298 0.63 -2.0 2,671,239 - Psyr_2298 0.63 +0.8 2,671,522 - Psyr_2298 0.79 +0.2 2,671,522 - Psyr_2298 0.79 -2.0 2,671,772 + -0.2 2,671,772 + +0.8 2,671,773 - +1.6 2,671,813 + +2.3 2,672,033 + +1.1 2,672,051 + -0.8 2,672,210 + Psyr_2299 0.17 -1.0 2,672,249 + Psyr_2299 0.20 -1.6 2,672,265 - Psyr_2299 0.21 -0.8 2,672,339 + Psyr_2299 0.26 +0.6 2,672,340 - Psyr_2299 0.26 -2.7 2,672,340 - Psyr_2299 0.26 +0.6 2,672,419 - Psyr_2299 0.32 -1.1 2,672,540 - Psyr_2299 0.40 -0.9 2,672,711 + Psyr_2299 0.52 -0.0 2,672,712 - Psyr_2299 0.52 +1.4 2,672,712 - Psyr_2299 0.52 +0.2 2,672,816 - Psyr_2299 0.60 +0.8 2,672,816 - Psyr_2299 0.60 -0.4 2,672,849 + Psyr_2299 0.62 -0.4 2,672,849 + Psyr_2299 0.62 -0.0 2,672,909 - Psyr_2299 0.66 +2.6 2,672,963 + Psyr_2299 0.70 -1.8 2,672,972 + Psyr_2299 0.71 +0.6 2,673,005 + Psyr_2299 0.73 -0.8 2,673,005 + Psyr_2299 0.73 -0.1 2,673,005 + Psyr_2299 0.73 -1.2 2,673,196 - Psyr_2299 0.86 -1.1 2,673,361 - +0.6 2,673,479 + -0.4 2,673,537 + +1.2 2,673,670 + Psyr_2300 0.23 -0.8 2,673,674 + Psyr_2300 0.24 -1.0 2,673,674 + Psyr_2300 0.24 +0.1 2,673,678 + Psyr_2300 0.24 +1.6 2,673,678 + Psyr_2300 0.24 +1.2 2,673,849 - Psyr_2300 0.42 +1.3 2,674,150 + Psyr_2300 0.73 -0.8 2,674,150 + Psyr_2300 0.73 -2.2 2,674,201 + Psyr_2300 0.78 +0.1 2,674,201 + Psyr_2300 0.78 -0.8 2,674,337 - +1.0
Or see this region's nucleotide sequence