Experiment: KB with Nalidixic 12.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2224 and Psyr_2225 overlap by 19 nucleotides Psyr_2225 and Psyr_2226 overlap by 4 nucleotides Psyr_2226 and Psyr_2227 are separated by 297 nucleotides
Psyr_2224: Psyr_2224 - FAD dependent oxidoreductase, at 2,585,630 to 2,586,871
_2224
Psyr_2225: Psyr_2225 - methenyltetrahydrofolate cyclohydrolase / 5,10-methylenetetrahydrofolate dehydrogenase (NADP+), at 2,586,853 to 2,587,755
_2225
Psyr_2226: Psyr_2226 - formyltetrahydrofolate deformylase, at 2,587,752 to 2,588,618
_2226
Psyr_2227: Psyr_2227 - conserved hypothetical protein, at 2,588,916 to 2,589,371
_2227
Position (kb)
2587
2588
2589 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2586.857 kb on + strand at 2586.948 kb on + strand, within Psyr_2225 at 2587.349 kb on - strand, within Psyr_2225 at 2587.607 kb on - strand, within Psyr_2225 at 2587.607 kb on - strand, within Psyr_2225 at 2587.610 kb on + strand, within Psyr_2225 at 2587.610 kb on + strand, within Psyr_2225 at 2587.610 kb on + strand, within Psyr_2225 at 2587.807 kb on + strand at 2587.807 kb on + strand at 2587.807 kb on + strand at 2587.808 kb on - strand at 2587.808 kb on - strand at 2587.830 kb on + strand at 2587.942 kb on + strand at 2587.943 kb on - strand, within Psyr_2226 at 2588.188 kb on + strand, within Psyr_2226 at 2588.188 kb on + strand, within Psyr_2226 at 2588.189 kb on - strand, within Psyr_2226 at 2588.485 kb on + strand, within Psyr_2226 at 2588.486 kb on - strand at 2588.486 kb on - strand, within Psyr_2226 at 2588.518 kb on + strand, within Psyr_2226 at 2588.519 kb on - strand, within Psyr_2226 at 2588.519 kb on - strand, within Psyr_2226 at 2588.736 kb on + strand at 2588.736 kb on + strand at 2588.736 kb on + strand at 2588.736 kb on + strand at 2588.737 kb on - strand at 2588.737 kb on - strand at 2588.737 kb on - strand at 2588.889 kb on - strand at 2588.889 kb on - strand at 2588.918 kb on - strand at 2588.918 kb on - strand at 2588.918 kb on - strand at 2588.967 kb on - strand, within Psyr_2227 at 2588.987 kb on + strand, within Psyr_2227 at 2589.282 kb on + strand, within Psyr_2227 at 2589.332 kb on + strand at 2589.332 kb on + strand at 2589.409 kb on + strand at 2589.410 kb on - strand at 2589.418 kb on + strand at 2589.443 kb on + strand at 2589.453 kb on + strand at 2589.453 kb on + strand at 2589.453 kb on + strand at 2589.453 kb on + strand at 2589.454 kb on - strand at 2589.454 kb on - strand at 2589.457 kb on + strand at 2589.457 kb on + strand at 2589.474 kb on + strand at 2589.477 kb on - strand at 2589.521 kb on + strand at 2589.521 kb on + strand at 2589.522 kb on - strand at 2589.522 kb on - strand at 2589.522 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Nalidixic 12.5 ug/ml remove 2,586,857 + -2.2 2,586,948 + Psyr_2225 0.11 +0.2 2,587,349 - Psyr_2225 0.55 -0.5 2,587,607 - Psyr_2225 0.83 -2.2 2,587,607 - Psyr_2225 0.83 -0.0 2,587,610 + Psyr_2225 0.84 -2.2 2,587,610 + Psyr_2225 0.84 -1.3 2,587,610 + Psyr_2225 0.84 +0.8 2,587,807 + -0.5 2,587,807 + +1.1 2,587,807 + -1.5 2,587,808 - +0.7 2,587,808 - +0.2 2,587,830 + -0.3 2,587,942 + +0.5 2,587,943 - Psyr_2226 0.22 +0.4 2,588,188 + Psyr_2226 0.50 -0.6 2,588,188 + Psyr_2226 0.50 -0.7 2,588,189 - Psyr_2226 0.50 -0.6 2,588,485 + Psyr_2226 0.85 -0.4 2,588,486 - +2.5 2,588,486 - Psyr_2226 0.85 -1.7 2,588,518 + Psyr_2226 0.88 -0.6 2,588,519 - Psyr_2226 0.88 -1.4 2,588,519 - Psyr_2226 0.88 -0.0 2,588,736 + -0.6 2,588,736 + +1.2 2,588,736 + +0.1 2,588,736 + -1.3 2,588,737 - -0.6 2,588,737 - +0.1 2,588,737 - +1.1 2,588,889 - +0.3 2,588,889 - +0.5 2,588,918 - -0.0 2,588,918 - -0.0 2,588,918 - -0.5 2,588,967 - Psyr_2227 0.11 -2.4 2,588,987 + Psyr_2227 0.16 -0.0 2,589,282 + Psyr_2227 0.80 -1.0 2,589,332 + -0.4 2,589,332 + +0.0 2,589,409 + +0.1 2,589,410 - +1.0 2,589,418 + -1.1 2,589,443 + -0.5 2,589,453 + -0.6 2,589,453 + +0.7 2,589,453 + -0.0 2,589,453 + -0.9 2,589,454 - -2.2 2,589,454 - -1.6 2,589,457 + +1.5 2,589,457 + +0.3 2,589,474 + +1.3 2,589,477 - -1.9 2,589,521 + -0.5 2,589,521 + -1.8 2,589,522 - -1.3 2,589,522 - -0.2 2,589,522 - -0.0
Or see this region's nucleotide sequence