Experiment: KB with Nalidixic 12.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1958 and Psyr_1959 are separated by 14 nucleotides Psyr_1959 and Psyr_1960 overlap by 20 nucleotides
Psyr_1958: Psyr_1958 - Non-ribosomal peptide synthase:Amino acid adenylation, at 2,244,792 to 2,252,792
_1958
Psyr_1959: Psyr_1959 - Amino acid adenylation, at 2,252,807 to 2,259,271
_1959
Psyr_1960: Psyr_1960 - Non-ribosomal peptide synthase:Amino acid adenylation, at 2,259,252 to 2,267,903
_1960
Position (kb)
2252
2253
2254
2255
2256
2257
2258
2259
2260 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2251.825 kb on - strand, within Psyr_1958 at 2252.137 kb on - strand at 2252.325 kb on - strand at 2252.356 kb on - strand at 2252.356 kb on - strand at 2252.356 kb on - strand at 2253.006 kb on - strand at 2253.209 kb on + strand at 2253.225 kb on + strand at 2253.225 kb on + strand at 2253.225 kb on + strand at 2253.226 kb on - strand at 2253.300 kb on + strand at 2253.300 kb on + strand at 2253.300 kb on + strand at 2253.300 kb on + strand at 2253.301 kb on - strand at 2253.301 kb on - strand at 2253.301 kb on - strand at 2253.301 kb on - strand at 2253.377 kb on + strand at 2253.575 kb on + strand, within Psyr_1959 at 2253.575 kb on + strand, within Psyr_1959 at 2253.723 kb on + strand, within Psyr_1959 at 2253.724 kb on - strand, within Psyr_1959 at 2253.724 kb on - strand, within Psyr_1959 at 2253.724 kb on - strand, within Psyr_1959 at 2254.115 kb on + strand, within Psyr_1959 at 2254.116 kb on - strand, within Psyr_1959 at 2254.211 kb on - strand, within Psyr_1959 at 2254.734 kb on - strand, within Psyr_1959 at 2254.743 kb on - strand, within Psyr_1959 at 2254.743 kb on - strand, within Psyr_1959 at 2254.743 kb on - strand, within Psyr_1959 at 2255.486 kb on + strand, within Psyr_1959 at 2255.487 kb on - strand, within Psyr_1959 at 2255.706 kb on - strand, within Psyr_1959 at 2256.161 kb on + strand, within Psyr_1959 at 2256.161 kb on + strand, within Psyr_1959 at 2256.162 kb on - strand, within Psyr_1959 at 2256.162 kb on - strand, within Psyr_1959 at 2256.162 kb on - strand, within Psyr_1959 at 2257.685 kb on + strand, within Psyr_1959 at 2257.685 kb on + strand, within Psyr_1959 at 2257.686 kb on - strand, within Psyr_1959 at 2257.686 kb on - strand, within Psyr_1959 at 2257.686 kb on - strand, within Psyr_1959 at 2257.728 kb on - strand, within Psyr_1959 at 2257.728 kb on - strand, within Psyr_1959 at 2257.851 kb on - strand, within Psyr_1959 at 2257.898 kb on + strand, within Psyr_1959 at 2257.899 kb on - strand, within Psyr_1959 at 2257.908 kb on - strand, within Psyr_1959 at 2258.251 kb on - strand, within Psyr_1959 at 2258.251 kb on - strand, within Psyr_1959 at 2258.321 kb on + strand, within Psyr_1959 at 2258.330 kb on + strand, within Psyr_1959 at 2258.486 kb on + strand, within Psyr_1959 at 2258.486 kb on + strand, within Psyr_1959 at 2258.487 kb on - strand, within Psyr_1959 at 2258.591 kb on + strand, within Psyr_1959 at 2258.787 kb on - strand at 2258.861 kb on + strand at 2258.973 kb on - strand at 2259.420 kb on - strand at 2259.432 kb on + strand at 2259.615 kb on + strand at 2259.615 kb on + strand at 2259.778 kb on + strand at 2259.779 kb on - strand at 2259.779 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Nalidixic 12.5 ug/ml remove 2,251,825 - Psyr_1958 0.88 -0.6 2,252,137 - +0.7 2,252,325 - +0.2 2,252,356 - -0.9 2,252,356 - +1.4 2,252,356 - -0.9 2,253,006 - +0.1 2,253,209 + +1.4 2,253,225 + +0.5 2,253,225 + +0.4 2,253,225 + +0.3 2,253,226 - +1.4 2,253,300 + -0.6 2,253,300 + +0.8 2,253,300 + +0.8 2,253,300 + +1.4 2,253,301 - -0.2 2,253,301 - +0.6 2,253,301 - -0.6 2,253,301 - -1.8 2,253,377 + +3.4 2,253,575 + Psyr_1959 0.12 +0.1 2,253,575 + Psyr_1959 0.12 -0.2 2,253,723 + Psyr_1959 0.14 +0.8 2,253,724 - Psyr_1959 0.14 +0.9 2,253,724 - Psyr_1959 0.14 -0.3 2,253,724 - Psyr_1959 0.14 -0.4 2,254,115 + Psyr_1959 0.20 -0.2 2,254,116 - Psyr_1959 0.20 +1.4 2,254,211 - Psyr_1959 0.22 +0.4 2,254,734 - Psyr_1959 0.30 +0.1 2,254,743 - Psyr_1959 0.30 -0.1 2,254,743 - Psyr_1959 0.30 +0.3 2,254,743 - Psyr_1959 0.30 -0.8 2,255,486 + Psyr_1959 0.41 -0.2 2,255,487 - Psyr_1959 0.41 +0.4 2,255,706 - Psyr_1959 0.45 -0.7 2,256,161 + Psyr_1959 0.52 -0.9 2,256,161 + Psyr_1959 0.52 -1.8 2,256,162 - Psyr_1959 0.52 +2.6 2,256,162 - Psyr_1959 0.52 -1.0 2,256,162 - Psyr_1959 0.52 +1.0 2,257,685 + Psyr_1959 0.75 -0.2 2,257,685 + Psyr_1959 0.75 -1.8 2,257,686 - Psyr_1959 0.75 -0.0 2,257,686 - Psyr_1959 0.75 +0.8 2,257,686 - Psyr_1959 0.75 -0.6 2,257,728 - Psyr_1959 0.76 +1.1 2,257,728 - Psyr_1959 0.76 -1.3 2,257,851 - Psyr_1959 0.78 +0.2 2,257,898 + Psyr_1959 0.79 +0.2 2,257,899 - Psyr_1959 0.79 +0.1 2,257,908 - Psyr_1959 0.79 +0.2 2,258,251 - Psyr_1959 0.84 -0.4 2,258,251 - Psyr_1959 0.84 +1.4 2,258,321 + Psyr_1959 0.85 -0.5 2,258,330 + Psyr_1959 0.85 +0.1 2,258,486 + Psyr_1959 0.88 -0.2 2,258,486 + Psyr_1959 0.88 +1.6 2,258,487 - Psyr_1959 0.88 -1.9 2,258,591 + Psyr_1959 0.89 +0.6 2,258,787 - +0.7 2,258,861 + +0.5 2,258,973 - -0.6 2,259,420 - -1.6 2,259,432 + +1.1 2,259,615 + -0.9 2,259,615 + -1.9 2,259,778 + -0.6 2,259,779 - +0.1 2,259,779 - +1.0
Or see this region's nucleotide sequence