Experiment: KB with Nalidixic 12.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1067 and Psyr_1068 overlap by 4 nucleotides Psyr_1068 and Psyr_1069 are separated by 2 nucleotides Psyr_1069 and Psyr_1070 are separated by 3 nucleotides Psyr_1070 and Psyr_1071 are separated by 115 nucleotides
Psyr_1067: Psyr_1067 - GTP cyclohydrolase subunit MoaC, at 1,211,047 to 1,211,532
_1067
Psyr_1068: Psyr_1068 - molybdopterin synthase subunit MoaD, at 1,211,529 to 1,211,774
_1068
Psyr_1069: Psyr_1069 - molybdopterin synthase subunit MoaE, at 1,211,777 to 1,212,226
_1069
Psyr_1070: Psyr_1070 - Helicase, C-terminal:DEAD/DEAH box helicase, N-terminal, at 1,212,230 to 1,213,687
_1070
Psyr_1071: Psyr_1071 - Carboxylesterase, at 1,213,803 to 1,214,462
_1071
Position (kb) 1212
1213
1214 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 1211.416 kb on + strand, within Psyr_1067 at 1211.416 kb on + strand, within Psyr_1067 at 1211.417 kb on - strand, within Psyr_1067 at 1211.417 kb on - strand, within Psyr_1067 at 1211.422 kb on - strand, within Psyr_1067 at 1211.879 kb on + strand, within Psyr_1069 at 1211.879 kb on + strand, within Psyr_1069 at 1211.879 kb on + strand, within Psyr_1069 at 1211.879 kb on + strand, within Psyr_1069 at 1211.880 kb on - strand, within Psyr_1069 at 1211.880 kb on - strand, within Psyr_1069 at 1211.880 kb on - strand, within Psyr_1069 at 1212.001 kb on - strand, within Psyr_1069 at 1212.013 kb on - strand, within Psyr_1069 at 1212.224 kb on + strand at 1212.224 kb on + strand at 1212.224 kb on + strand at 1212.225 kb on - strand at 1212.225 kb on - strand at 1212.225 kb on - strand at 1212.225 kb on - strand at 1212.328 kb on - strand at 1212.328 kb on - strand at 1212.328 kb on - strand at 1212.405 kb on + strand, within Psyr_1070 at 1212.682 kb on - strand, within Psyr_1070 at 1212.682 kb on - strand, within Psyr_1070 at 1212.693 kb on + strand, within Psyr_1070 at 1212.693 kb on + strand, within Psyr_1070 at 1212.693 kb on + strand, within Psyr_1070 at 1212.693 kb on + strand, within Psyr_1070 at 1212.693 kb on + strand, within Psyr_1070 at 1212.694 kb on - strand at 1212.694 kb on - strand, within Psyr_1070 at 1212.694 kb on - strand, within Psyr_1070 at 1212.694 kb on - strand, within Psyr_1070 at 1212.694 kb on - strand, within Psyr_1070 at 1212.694 kb on - strand, within Psyr_1070 at 1212.717 kb on + strand, within Psyr_1070 at 1212.717 kb on + strand, within Psyr_1070 at 1212.717 kb on + strand, within Psyr_1070 at 1212.717 kb on + strand, within Psyr_1070 at 1212.717 kb on + strand, within Psyr_1070 at 1212.717 kb on + strand, within Psyr_1070 at 1212.717 kb on + strand, within Psyr_1070 at 1212.717 kb on + strand, within Psyr_1070 at 1212.718 kb on - strand, within Psyr_1070 at 1212.718 kb on - strand, within Psyr_1070 at 1212.718 kb on - strand, within Psyr_1070 at 1212.718 kb on - strand, within Psyr_1070 at 1212.718 kb on - strand, within Psyr_1070 at 1212.718 kb on - strand, within Psyr_1070 at 1212.721 kb on + strand, within Psyr_1070 at 1212.721 kb on + strand, within Psyr_1070 at 1212.721 kb on + strand, within Psyr_1070 at 1212.721 kb on + strand, within Psyr_1070 at 1212.721 kb on + strand, within Psyr_1070 at 1212.721 kb on + strand, within Psyr_1070 at 1212.721 kb on + strand, within Psyr_1070 at 1212.721 kb on + strand, within Psyr_1070 at 1212.721 kb on + strand, within Psyr_1070 at 1212.721 kb on + strand, within Psyr_1070 at 1212.721 kb on + strand, within Psyr_1070 at 1212.721 kb on + strand, within Psyr_1070 at 1212.722 kb on - strand, within Psyr_1070 at 1212.722 kb on - strand, within Psyr_1070 at 1212.722 kb on - strand, within Psyr_1070 at 1212.722 kb on - strand, within Psyr_1070 at 1212.722 kb on - strand, within Psyr_1070 at 1212.722 kb on - strand, within Psyr_1070 at 1212.722 kb on - strand, within Psyr_1070 at 1212.722 kb on - strand, within Psyr_1070 at 1212.722 kb on - strand, within Psyr_1070 at 1212.722 kb on - strand, within Psyr_1070 at 1212.722 kb on - strand, within Psyr_1070 at 1212.722 kb on - strand, within Psyr_1070 at 1212.722 kb on - strand, within Psyr_1070 at 1212.809 kb on + strand, within Psyr_1070 at 1212.810 kb on - strand, within Psyr_1070 at 1212.810 kb on - strand, within Psyr_1070 at 1212.810 kb on - strand, within Psyr_1070 at 1212.887 kb on + strand, within Psyr_1070 at 1212.887 kb on + strand, within Psyr_1070 at 1212.887 kb on + strand, within Psyr_1070 at 1212.953 kb on - strand, within Psyr_1070 at 1213.098 kb on + strand, within Psyr_1070 at 1213.098 kb on + strand, within Psyr_1070 at 1213.098 kb on + strand, within Psyr_1070 at 1213.099 kb on - strand, within Psyr_1070 at 1213.099 kb on - strand, within Psyr_1070 at 1213.099 kb on - strand, within Psyr_1070 at 1213.185 kb on + strand, within Psyr_1070 at 1213.185 kb on + strand, within Psyr_1070 at 1213.186 kb on - strand, within Psyr_1070 at 1213.186 kb on - strand, within Psyr_1070 at 1213.186 kb on - strand, within Psyr_1070 at 1213.609 kb on + strand at 1213.610 kb on - strand at 1213.667 kb on - strand at 1213.711 kb on - strand at 1213.725 kb on - strand at 1213.725 kb on - strand at 1213.767 kb on + strand at 1213.767 kb on + strand at 1213.768 kb on - strand at 1213.896 kb on + strand, within Psyr_1071 at 1213.897 kb on - strand, within Psyr_1071 at 1214.030 kb on + strand, within Psyr_1071 at 1214.030 kb on + strand, within Psyr_1071 at 1214.031 kb on - strand, within Psyr_1071 at 1214.031 kb on - strand, within Psyr_1071 at 1214.031 kb on - strand, within Psyr_1071 at 1214.038 kb on + strand, within Psyr_1071 at 1214.038 kb on + strand, within Psyr_1071 at 1214.038 kb on + strand, within Psyr_1071 at 1214.038 kb on + strand, within Psyr_1071 at 1214.039 kb on - strand, within Psyr_1071 at 1214.039 kb on - strand, within Psyr_1071 at 1214.039 kb on - strand, within Psyr_1071 at 1214.039 kb on - strand, within Psyr_1071 at 1214.039 kb on - strand, within Psyr_1071 at 1214.039 kb on - strand, within Psyr_1071 at 1214.261 kb on - strand, within Psyr_1071 at 1214.272 kb on - strand, within Psyr_1071 at 1214.272 kb on - strand, within Psyr_1071 at 1214.323 kb on - strand, within Psyr_1071 at 1214.323 kb on - strand, within Psyr_1071 at 1214.323 kb on - strand, within Psyr_1071 at 1214.380 kb on + strand, within Psyr_1071 at 1214.381 kb on - strand, within Psyr_1071 at 1214.381 kb on - strand, within Psyr_1071 at 1214.411 kb on + strand at 1214.506 kb on + strand at 1214.506 kb on + strand at 1214.507 kb on - strand at 1214.551 kb on + strand at 1214.588 kb on - strand at 1214.588 kb on - strand at 1214.658 kb on + strand at 1214.658 kb on + strand at 1214.658 kb on + strand at 1214.658 kb on + strand at 1214.658 kb on + strand at 1214.658 kb on + strand at 1214.658 kb on + strand at 1214.658 kb on + strand at 1214.658 kb on + strand at 1214.658 kb on + strand at 1214.659 kb on - strand at 1214.659 kb on - strand at 1214.659 kb on - strand at 1214.659 kb on - strand at 1214.659 kb on - strand at 1214.659 kb on - strand at 1214.659 kb on - strand at 1214.659 kb on - strand at 1214.659 kb on - strand at 1214.660 kb on + strand at 1214.660 kb on + strand at 1214.660 kb on + strand at 1214.660 kb on + strand at 1214.661 kb on - strand at 1214.661 kb on - strand at 1214.661 kb on - strand at 1214.661 kb on - strand at 1214.666 kb on + strand at 1214.666 kb on + strand at 1214.667 kb on - strand at 1214.669 kb on + strand at 1214.669 kb on + strand at 1214.669 kb on + strand at 1214.669 kb on + strand at 1214.669 kb on + strand at 1214.669 kb on + strand at 1214.669 kb on + strand at 1214.669 kb on + strand at 1214.670 kb on - strand at 1214.670 kb on - strand at 1214.670 kb on - strand at 1214.670 kb on - strand at 1214.670 kb on - strand at 1214.670 kb on - strand at 1214.670 kb on - strand at 1214.670 kb on - strand at 1214.673 kb on + strand at 1214.673 kb on + strand at 1214.673 kb on + strand at 1214.673 kb on + strand at 1214.673 kb on + strand at 1214.673 kb on + strand at 1214.673 kb on + strand at 1214.673 kb on + strand at 1214.674 kb on - strand at 1214.674 kb on - strand at 1214.674 kb on - strand at 1214.674 kb on - strand at 1214.675 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Nalidixic 12.5 ug/ml remove 1,211,416 + Psyr_1067 0.76 -0.9 1,211,416 + Psyr_1067 0.76 -2.5 1,211,417 - Psyr_1067 0.76 -0.5 1,211,417 - Psyr_1067 0.76 -0.3 1,211,422 - Psyr_1067 0.77 -0.3 1,211,879 + Psyr_1069 0.23 -1.1 1,211,879 + Psyr_1069 0.23 -0.6 1,211,879 + Psyr_1069 0.23 -0.7 1,211,879 + Psyr_1069 0.23 -0.1 1,211,880 - Psyr_1069 0.23 -1.3 1,211,880 - Psyr_1069 0.23 -1.0 1,211,880 - Psyr_1069 0.23 +0.9 1,212,001 - Psyr_1069 0.50 -1.3 1,212,013 - Psyr_1069 0.52 -2.3 1,212,224 + +0.4 1,212,224 + +0.7 1,212,224 + +0.6 1,212,225 - -2.1 1,212,225 - +0.3 1,212,225 - +1.1 1,212,225 - +0.1 1,212,328 - -0.9 1,212,328 - -2.6 1,212,328 - +0.5 1,212,405 + Psyr_1070 0.12 -0.9 1,212,682 - Psyr_1070 0.31 +0.4 1,212,682 - Psyr_1070 0.31 -0.6 1,212,693 + Psyr_1070 0.32 +0.2 1,212,693 + Psyr_1070 0.32 +0.9 1,212,693 + Psyr_1070 0.32 -0.6 1,212,693 + Psyr_1070 0.32 -0.1 1,212,693 + Psyr_1070 0.32 +0.9 1,212,694 - +1.9 1,212,694 - Psyr_1070 0.32 +0.6 1,212,694 - Psyr_1070 0.32 -2.1 1,212,694 - Psyr_1070 0.32 -0.2 1,212,694 - Psyr_1070 0.32 -0.3 1,212,694 - Psyr_1070 0.32 +0.5 1,212,717 + Psyr_1070 0.33 -0.5 1,212,717 + Psyr_1070 0.33 -2.3 1,212,717 + Psyr_1070 0.33 +4.2 1,212,717 + Psyr_1070 0.33 +0.7 1,212,717 + Psyr_1070 0.33 +0.9 1,212,717 + Psyr_1070 0.33 -0.1 1,212,717 + Psyr_1070 0.33 -2.5 1,212,717 + Psyr_1070 0.33 -2.1 1,212,718 - Psyr_1070 0.33 +0.9 1,212,718 - Psyr_1070 0.33 -2.1 1,212,718 - Psyr_1070 0.33 -0.7 1,212,718 - Psyr_1070 0.33 +0.9 1,212,718 - Psyr_1070 0.33 +1.6 1,212,718 - Psyr_1070 0.33 +0.2 1,212,721 + Psyr_1070 0.34 -1.7 1,212,721 + Psyr_1070 0.34 -1.7 1,212,721 + Psyr_1070 0.34 -1.1 1,212,721 + Psyr_1070 0.34 -1.4 1,212,721 + Psyr_1070 0.34 -0.7 1,212,721 + Psyr_1070 0.34 -0.3 1,212,721 + Psyr_1070 0.34 +1.6 1,212,721 + Psyr_1070 0.34 +0.6 1,212,721 + Psyr_1070 0.34 -2.4 1,212,721 + Psyr_1070 0.34 -1.1 1,212,721 + Psyr_1070 0.34 +0.1 1,212,721 + Psyr_1070 0.34 +0.0 1,212,722 - Psyr_1070 0.34 +0.2 1,212,722 - Psyr_1070 0.34 -1.1 1,212,722 - Psyr_1070 0.34 +1.0 1,212,722 - Psyr_1070 0.34 +1.2 1,212,722 - Psyr_1070 0.34 +0.5 1,212,722 - Psyr_1070 0.34 -1.0 1,212,722 - Psyr_1070 0.34 +0.6 1,212,722 - Psyr_1070 0.34 -0.7 1,212,722 - Psyr_1070 0.34 -0.1 1,212,722 - Psyr_1070 0.34 -0.3 1,212,722 - Psyr_1070 0.34 -0.7 1,212,722 - Psyr_1070 0.34 -1.3 1,212,722 - Psyr_1070 0.34 -0.1 1,212,809 + Psyr_1070 0.40 -1.3 1,212,810 - Psyr_1070 0.40 -1.6 1,212,810 - Psyr_1070 0.40 -0.5 1,212,810 - Psyr_1070 0.40 -0.1 1,212,887 + Psyr_1070 0.45 +0.8 1,212,887 + Psyr_1070 0.45 +0.5 1,212,887 + Psyr_1070 0.45 +0.3 1,212,953 - Psyr_1070 0.50 -1.7 1,213,098 + Psyr_1070 0.60 -1.5 1,213,098 + Psyr_1070 0.60 -1.0 1,213,098 + Psyr_1070 0.60 -0.7 1,213,099 - Psyr_1070 0.60 -3.2 1,213,099 - Psyr_1070 0.60 +0.3 1,213,099 - Psyr_1070 0.60 -0.5 1,213,185 + Psyr_1070 0.66 +0.1 1,213,185 + Psyr_1070 0.66 -0.7 1,213,186 - Psyr_1070 0.66 -1.5 1,213,186 - Psyr_1070 0.66 +0.0 1,213,186 - Psyr_1070 0.66 +0.9 1,213,609 + +0.9 1,213,610 - +0.9 1,213,667 - +0.9 1,213,711 - -0.7 1,213,725 - -1.4 1,213,725 - -0.6 1,213,767 + +0.1 1,213,767 + +0.7 1,213,768 - +0.1 1,213,896 + Psyr_1071 0.14 -0.6 1,213,897 - Psyr_1071 0.14 +0.3 1,214,030 + Psyr_1071 0.34 +0.2 1,214,030 + Psyr_1071 0.34 -1.1 1,214,031 - Psyr_1071 0.35 -0.1 1,214,031 - Psyr_1071 0.35 +0.5 1,214,031 - Psyr_1071 0.35 -0.1 1,214,038 + Psyr_1071 0.36 -0.0 1,214,038 + Psyr_1071 0.36 -0.1 1,214,038 + Psyr_1071 0.36 -0.5 1,214,038 + Psyr_1071 0.36 +2.9 1,214,039 - Psyr_1071 0.36 +0.4 1,214,039 - Psyr_1071 0.36 +1.1 1,214,039 - Psyr_1071 0.36 +1.0 1,214,039 - Psyr_1071 0.36 +0.4 1,214,039 - Psyr_1071 0.36 +0.1 1,214,039 - Psyr_1071 0.36 +0.3 1,214,261 - Psyr_1071 0.69 -3.1 1,214,272 - Psyr_1071 0.71 -0.4 1,214,272 - Psyr_1071 0.71 +1.6 1,214,323 - Psyr_1071 0.79 +0.6 1,214,323 - Psyr_1071 0.79 +0.5 1,214,323 - Psyr_1071 0.79 -0.0 1,214,380 + Psyr_1071 0.87 -0.6 1,214,381 - Psyr_1071 0.88 +0.6 1,214,381 - Psyr_1071 0.88 +1.4 1,214,411 + +0.4 1,214,506 + +1.8 1,214,506 + -0.5 1,214,507 - -0.1 1,214,551 + -1.0 1,214,588 - +2.2 1,214,588 - -1.4 1,214,658 + +0.3 1,214,658 + -0.1 1,214,658 + -1.0 1,214,658 + +0.0 1,214,658 + -0.8 1,214,658 + -0.1 1,214,658 + -0.3 1,214,658 + -0.7 1,214,658 + -2.1 1,214,658 + +2.3 1,214,659 - -1.6 1,214,659 - +0.1 1,214,659 - +0.9 1,214,659 - -0.1 1,214,659 - +0.2 1,214,659 - +0.7 1,214,659 - -1.5 1,214,659 - +0.0 1,214,659 - -0.6 1,214,660 + -0.2 1,214,660 + -0.1 1,214,660 + +0.6 1,214,660 + -1.1 1,214,661 - -0.5 1,214,661 - +2.0 1,214,661 - -0.3 1,214,661 - -0.6 1,214,666 + -2.5 1,214,666 + -0.1 1,214,667 - +0.7 1,214,669 + -0.1 1,214,669 + -1.2 1,214,669 + -0.8 1,214,669 + -0.1 1,214,669 + -0.5 1,214,669 + -0.1 1,214,669 + +0.2 1,214,669 + +0.9 1,214,670 - -0.1 1,214,670 - -0.8 1,214,670 - -1.1 1,214,670 - +0.3 1,214,670 - -2.1 1,214,670 - +0.7 1,214,670 - +0.2 1,214,670 - +1.4 1,214,673 + +1.9 1,214,673 + -1.5 1,214,673 + +1.0 1,214,673 + -0.4 1,214,673 + -1.1 1,214,673 + +2.2 1,214,673 + -2.3 1,214,673 + +0.4 1,214,674 - -1.5 1,214,674 - -0.9 1,214,674 - -0.4 1,214,674 - +1.9 1,214,675 + +0.9
Or see this region's nucleotide sequence