Experiment: KB with Nalidixic 12.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0165 and Psyr_0166 are separated by 563 nucleotides Psyr_0166 and Psyr_0167 are separated by 5 nucleotides Psyr_0167 and Psyr_0168 are separated by 370 nucleotides
Psyr_0165: Psyr_0165 - GCN5-related N-acetyltransferase, at 179,100 to 179,543
_0165
Psyr_0166: Psyr_0166 - hypothetical protein, at 180,107 to 181,417
_0166
Psyr_0167: Psyr_0167 - hypothetical protein, at 181,423 to 181,845
_0167
Psyr_0168: Psyr_0168 - Phosphoenolpyruvate carboxykinase (ATP), at 182,216 to 183,760
_0168
Position (kb) 180
181
182 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 179.128 kb on + strand at 179.128 kb on + strand at 179.128 kb on + strand at 179.128 kb on + strand at 179.128 kb on + strand at 179.128 kb on + strand at 179.128 kb on + strand at 179.128 kb on + strand at 179.128 kb on + strand at 179.128 kb on + strand at 179.128 kb on + strand at 179.129 kb on - strand at 179.129 kb on - strand at 179.129 kb on - strand at 179.129 kb on - strand at 179.129 kb on - strand at 179.129 kb on - strand at 179.129 kb on - strand at 179.129 kb on - strand at 179.209 kb on + strand, within Psyr_0165 at 179.253 kb on + strand, within Psyr_0165 at 179.253 kb on + strand, within Psyr_0165 at 179.253 kb on + strand, within Psyr_0165 at 179.253 kb on + strand, within Psyr_0165 at 179.253 kb on + strand, within Psyr_0165 at 179.254 kb on - strand, within Psyr_0165 at 179.254 kb on - strand, within Psyr_0165 at 179.399 kb on + strand, within Psyr_0165 at 179.424 kb on + strand, within Psyr_0165 at 179.425 kb on - strand, within Psyr_0165 at 179.458 kb on + strand, within Psyr_0165 at 179.733 kb on - strand at 179.769 kb on + strand at 179.769 kb on + strand at 179.862 kb on + strand at 179.862 kb on + strand at 179.862 kb on + strand at 179.862 kb on + strand at 179.863 kb on - strand at 179.863 kb on - strand at 179.866 kb on + strand at 179.866 kb on + strand at 179.866 kb on + strand at 179.867 kb on - strand at 179.867 kb on - strand at 179.877 kb on + strand at 179.927 kb on - strand at 180.013 kb on + strand at 180.014 kb on - strand at 180.065 kb on + strand at 180.065 kb on + strand at 180.065 kb on + strand at 180.065 kb on + strand at 180.066 kb on - strand at 180.066 kb on - strand at 180.066 kb on - strand at 180.076 kb on + strand at 180.077 kb on - strand at 180.077 kb on - strand at 180.140 kb on + strand at 180.140 kb on + strand at 180.140 kb on + strand at 180.141 kb on - strand at 180.141 kb on - strand at 180.141 kb on - strand at 180.175 kb on - strand at 180.195 kb on + strand at 180.237 kb on - strand at 180.268 kb on - strand, within Psyr_0166 at 180.399 kb on + strand, within Psyr_0166 at 180.418 kb on + strand, within Psyr_0166 at 180.473 kb on - strand, within Psyr_0166 at 180.529 kb on + strand, within Psyr_0166 at 180.529 kb on + strand, within Psyr_0166 at 180.533 kb on + strand, within Psyr_0166 at 180.533 kb on + strand, within Psyr_0166 at 180.534 kb on - strand, within Psyr_0166 at 180.534 kb on - strand, within Psyr_0166 at 180.534 kb on - strand, within Psyr_0166 at 180.534 kb on - strand, within Psyr_0166 at 180.534 kb on - strand, within Psyr_0166 at 180.534 kb on - strand, within Psyr_0166 at 180.534 kb on - strand, within Psyr_0166 at 180.722 kb on - strand, within Psyr_0166 at 180.748 kb on + strand, within Psyr_0166 at 180.847 kb on - strand, within Psyr_0166 at 180.847 kb on - strand, within Psyr_0166 at 180.995 kb on + strand, within Psyr_0166 at 180.995 kb on + strand, within Psyr_0166 at 180.995 kb on + strand, within Psyr_0166 at 180.995 kb on + strand, within Psyr_0166 at 180.996 kb on - strand, within Psyr_0166 at 180.996 kb on - strand, within Psyr_0166 at 180.997 kb on + strand, within Psyr_0166 at 180.997 kb on + strand, within Psyr_0166 at 180.997 kb on + strand, within Psyr_0166 at 180.997 kb on + strand, within Psyr_0166 at 181.012 kb on + strand, within Psyr_0166 at 181.016 kb on + strand, within Psyr_0166 at 181.017 kb on - strand, within Psyr_0166 at 181.042 kb on + strand, within Psyr_0166 at 181.042 kb on + strand, within Psyr_0166 at 181.092 kb on - strand, within Psyr_0166 at 181.151 kb on - strand, within Psyr_0166 at 181.184 kb on + strand, within Psyr_0166 at 181.274 kb on + strand, within Psyr_0166 at 181.286 kb on + strand, within Psyr_0166 at 181.287 kb on - strand at 181.288 kb on + strand at 181.289 kb on - strand at 181.328 kb on - strand at 181.387 kb on + strand at 181.611 kb on + strand, within Psyr_0167 at 181.693 kb on + strand, within Psyr_0167 at 181.693 kb on + strand, within Psyr_0167 at 181.693 kb on + strand, within Psyr_0167 at 181.693 kb on + strand, within Psyr_0167 at 181.694 kb on - strand, within Psyr_0167 at 181.694 kb on - strand, within Psyr_0167 at 181.696 kb on - strand, within Psyr_0167 at 181.798 kb on - strand, within Psyr_0167 at 181.843 kb on + strand at 181.847 kb on + strand at 181.847 kb on + strand at 181.847 kb on + strand at 181.848 kb on - strand at 181.848 kb on - strand at 181.904 kb on + strand at 181.904 kb on + strand at 181.904 kb on + strand at 181.905 kb on - strand at 182.034 kb on - strand at 182.054 kb on - strand at 182.084 kb on + strand at 182.086 kb on + strand at 182.087 kb on - strand at 182.087 kb on - strand at 182.104 kb on - strand at 182.128 kb on - strand at 182.207 kb on + strand at 182.207 kb on + strand at 182.208 kb on - strand at 182.217 kb on + strand at 182.218 kb on - strand at 182.218 kb on - strand at 182.313 kb on + strand at 182.314 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Nalidixic 12.5 ug/ml remove 179,128 + +0.1 179,128 + -1.8 179,128 + -0.3 179,128 + -0.4 179,128 + -1.6 179,128 + +1.3 179,128 + -1.1 179,128 + -1.8 179,128 + +0.3 179,128 + -0.4 179,128 + -0.3 179,129 - -1.0 179,129 - -0.6 179,129 - -0.3 179,129 - -0.3 179,129 - -1.1 179,129 - -0.4 179,129 - +0.6 179,129 - -0.7 179,209 + Psyr_0165 0.25 -0.3 179,253 + Psyr_0165 0.34 -0.0 179,253 + Psyr_0165 0.34 +0.7 179,253 + Psyr_0165 0.34 +0.5 179,253 + Psyr_0165 0.34 -0.4 179,253 + Psyr_0165 0.34 -0.1 179,254 - Psyr_0165 0.35 +0.3 179,254 - Psyr_0165 0.35 -0.1 179,399 + Psyr_0165 0.67 +2.6 179,424 + Psyr_0165 0.73 +0.5 179,425 - Psyr_0165 0.73 -0.1 179,458 + Psyr_0165 0.81 +0.3 179,733 - -0.5 179,769 + +0.5 179,769 + +0.7 179,862 + -2.9 179,862 + -0.8 179,862 + -1.0 179,862 + -0.5 179,863 - -0.4 179,863 - -0.1 179,866 + +0.2 179,866 + +0.2 179,866 + -1.1 179,867 - -1.4 179,867 - -0.6 179,877 + -1.4 179,927 - -0.8 180,013 + -0.0 180,014 - -0.4 180,065 + +0.4 180,065 + -0.7 180,065 + -0.1 180,065 + +0.1 180,066 - -0.4 180,066 - -0.4 180,066 - -1.3 180,076 + +0.9 180,077 - -0.0 180,077 - +1.0 180,140 + +0.4 180,140 + -0.0 180,140 + +1.6 180,141 - -0.9 180,141 - -1.1 180,141 - -1.0 180,175 - +1.0 180,195 + -0.7 180,237 - -2.6 180,268 - Psyr_0166 0.12 -0.8 180,399 + Psyr_0166 0.22 -0.4 180,418 + Psyr_0166 0.24 -0.4 180,473 - Psyr_0166 0.28 +0.2 180,529 + Psyr_0166 0.32 -0.1 180,529 + Psyr_0166 0.32 -0.4 180,533 + Psyr_0166 0.32 -1.2 180,533 + Psyr_0166 0.32 -1.0 180,534 - Psyr_0166 0.33 -0.3 180,534 - Psyr_0166 0.33 -0.4 180,534 - Psyr_0166 0.33 -0.4 180,534 - Psyr_0166 0.33 -0.8 180,534 - Psyr_0166 0.33 -0.9 180,534 - Psyr_0166 0.33 -0.2 180,534 - Psyr_0166 0.33 -0.2 180,722 - Psyr_0166 0.47 +0.1 180,748 + Psyr_0166 0.49 -0.3 180,847 - Psyr_0166 0.56 -2.5 180,847 - Psyr_0166 0.56 -2.0 180,995 + Psyr_0166 0.68 +0.6 180,995 + Psyr_0166 0.68 -1.6 180,995 + Psyr_0166 0.68 +0.3 180,995 + Psyr_0166 0.68 -0.4 180,996 - Psyr_0166 0.68 -2.2 180,996 - Psyr_0166 0.68 -0.3 180,997 + Psyr_0166 0.68 -0.9 180,997 + Psyr_0166 0.68 -1.4 180,997 + Psyr_0166 0.68 -0.9 180,997 + Psyr_0166 0.68 -0.4 181,012 + Psyr_0166 0.69 -0.9 181,016 + Psyr_0166 0.69 +0.6 181,017 - Psyr_0166 0.69 -1.0 181,042 + Psyr_0166 0.71 -0.4 181,042 + Psyr_0166 0.71 -1.2 181,092 - Psyr_0166 0.75 +0.2 181,151 - Psyr_0166 0.80 -2.8 181,184 + Psyr_0166 0.82 +0.0 181,274 + Psyr_0166 0.89 +1.1 181,286 + Psyr_0166 0.90 -0.2 181,287 - -0.0 181,288 + -0.5 181,289 - -0.9 181,328 - -2.8 181,387 + +0.0 181,611 + Psyr_0167 0.44 -0.2 181,693 + Psyr_0167 0.64 +1.2 181,693 + Psyr_0167 0.64 -0.8 181,693 + Psyr_0167 0.64 +0.7 181,693 + Psyr_0167 0.64 +0.0 181,694 - Psyr_0167 0.64 -1.6 181,694 - Psyr_0167 0.64 +0.1 181,696 - Psyr_0167 0.65 +1.2 181,798 - Psyr_0167 0.89 -0.9 181,843 + -1.0 181,847 + +0.6 181,847 + -1.0 181,847 + -0.4 181,848 - -0.8 181,848 - -1.3 181,904 + -1.5 181,904 + +0.6 181,904 + +0.6 181,905 - -0.6 182,034 - -2.9 182,054 - -0.9 182,084 + +1.4 182,086 + +0.2 182,087 - -1.6 182,087 - -4.1 182,104 - -2.3 182,128 - -0.8 182,207 + -0.5 182,207 + +0.2 182,208 - -0.7 182,217 + -0.4 182,218 - +0.1 182,218 - +1.6 182,313 + +0.4 182,314 - +0.6
Or see this region's nucleotide sequence