Experiment: KB with Nalidixic 12.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0100 and Psyr_0101 are separated by 18 nucleotides Psyr_0101 and Psyr_0102 are separated by 418 nucleotides Psyr_0102 and Psyr_0103 are separated by 11 nucleotides
Psyr_0100: Psyr_0100 - conserved hypothetical protein, at 112,435 to 112,968
_0100
Psyr_0101: Psyr_0101 - Protein of unknown function DUF796, at 112,987 to 113,487
_0101
Psyr_0102: Psyr_0102 - hypothetical protein, at 113,906 to 114,502
_0102
Psyr_0103: Psyr_0103 - conserved hypothetical protein, at 114,514 to 114,891
_0103
Position (kb)
113
114
115 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 112.931 kb on - strand at 112.968 kb on + strand at 112.968 kb on + strand at 112.968 kb on + strand at 112.968 kb on + strand at 112.968 kb on + strand at 112.968 kb on + strand at 112.968 kb on + strand at 112.968 kb on + strand at 112.968 kb on + strand at 112.968 kb on + strand at 112.968 kb on + strand at 112.968 kb on + strand at 112.968 kb on + strand at 112.969 kb on - strand at 112.969 kb on - strand at 112.969 kb on - strand at 112.969 kb on - strand at 112.969 kb on - strand at 112.969 kb on - strand at 112.969 kb on - strand at 112.969 kb on - strand at 112.969 kb on - strand at 112.969 kb on - strand at 112.994 kb on + strand at 112.994 kb on + strand at 112.995 kb on - strand at 112.996 kb on + strand at 112.996 kb on + strand at 112.996 kb on + strand at 112.997 kb on - strand at 112.997 kb on - strand at 113.070 kb on + strand, within Psyr_0101 at 113.070 kb on + strand, within Psyr_0101 at 113.071 kb on - strand, within Psyr_0101 at 113.071 kb on - strand, within Psyr_0101 at 113.072 kb on + strand, within Psyr_0101 at 113.072 kb on + strand, within Psyr_0101 at 113.072 kb on + strand, within Psyr_0101 at 113.072 kb on + strand, within Psyr_0101 at 113.072 kb on + strand, within Psyr_0101 at 113.072 kb on + strand, within Psyr_0101 at 113.072 kb on + strand, within Psyr_0101 at 113.073 kb on - strand, within Psyr_0101 at 113.073 kb on - strand, within Psyr_0101 at 113.073 kb on - strand, within Psyr_0101 at 113.073 kb on - strand, within Psyr_0101 at 113.073 kb on - strand, within Psyr_0101 at 113.073 kb on - strand, within Psyr_0101 at 113.073 kb on - strand, within Psyr_0101 at 113.073 kb on - strand, within Psyr_0101 at 113.093 kb on + strand, within Psyr_0101 at 113.093 kb on + strand, within Psyr_0101 at 113.094 kb on - strand, within Psyr_0101 at 113.131 kb on + strand, within Psyr_0101 at 113.131 kb on + strand, within Psyr_0101 at 113.131 kb on + strand, within Psyr_0101 at 113.131 kb on + strand, within Psyr_0101 at 113.132 kb on - strand, within Psyr_0101 at 113.166 kb on + strand, within Psyr_0101 at 113.166 kb on + strand, within Psyr_0101 at 113.185 kb on + strand, within Psyr_0101 at 113.185 kb on + strand, within Psyr_0101 at 113.186 kb on - strand, within Psyr_0101 at 113.186 kb on - strand, within Psyr_0101 at 113.196 kb on - strand, within Psyr_0101 at 113.325 kb on + strand, within Psyr_0101 at 113.326 kb on - strand at 113.392 kb on + strand, within Psyr_0101 at 113.392 kb on + strand, within Psyr_0101 at 113.472 kb on - strand at 113.472 kb on - strand at 113.472 kb on - strand at 113.493 kb on + strand at 113.516 kb on - strand at 113.523 kb on - strand at 113.534 kb on + strand at 113.534 kb on + strand at 113.535 kb on - strand at 113.535 kb on - strand at 113.535 kb on - strand at 113.654 kb on - strand at 113.735 kb on + strand at 113.736 kb on - strand at 113.737 kb on + strand at 113.737 kb on + strand at 113.737 kb on + strand at 113.737 kb on + strand at 113.737 kb on + strand at 113.737 kb on + strand at 113.737 kb on + strand at 113.737 kb on + strand at 113.738 kb on - strand at 113.738 kb on - strand at 113.738 kb on - strand at 113.751 kb on + strand at 113.751 kb on + strand at 113.755 kb on + strand at 113.755 kb on + strand at 113.755 kb on + strand at 113.755 kb on + strand at 113.755 kb on + strand at 113.755 kb on + strand at 113.755 kb on + strand at 113.755 kb on + strand at 113.755 kb on + strand at 113.755 kb on + strand at 113.755 kb on + strand at 113.755 kb on + strand at 113.755 kb on + strand at 113.755 kb on + strand at 113.755 kb on + strand at 113.756 kb on - strand at 113.756 kb on - strand at 113.756 kb on - strand at 113.756 kb on - strand at 113.756 kb on - strand at 113.756 kb on - strand at 113.756 kb on - strand at 113.756 kb on - strand at 113.756 kb on - strand at 113.756 kb on - strand at 113.756 kb on - strand at 113.793 kb on + strand at 113.793 kb on + strand at 113.793 kb on + strand at 113.794 kb on - strand at 113.804 kb on - strand at 113.875 kb on + strand at 113.875 kb on + strand at 113.876 kb on - strand at 113.876 kb on - strand at 113.876 kb on - strand at 113.876 kb on - strand at 113.876 kb on - strand at 113.876 kb on - strand at 113.963 kb on + strand at 113.964 kb on - strand at 114.021 kb on + strand, within Psyr_0102 at 114.021 kb on + strand, within Psyr_0102 at 114.021 kb on + strand, within Psyr_0102 at 114.021 kb on + strand, within Psyr_0102 at 114.021 kb on + strand, within Psyr_0102 at 114.021 kb on + strand, within Psyr_0102 at 114.021 kb on + strand, within Psyr_0102 at 114.022 kb on - strand, within Psyr_0102 at 114.022 kb on - strand, within Psyr_0102 at 114.022 kb on - strand, within Psyr_0102 at 114.022 kb on - strand, within Psyr_0102 at 114.022 kb on - strand, within Psyr_0102 at 114.022 kb on - strand, within Psyr_0102 at 114.024 kb on + strand, within Psyr_0102 at 114.025 kb on - strand, within Psyr_0102 at 114.025 kb on - strand, within Psyr_0102 at 114.028 kb on + strand, within Psyr_0102 at 114.028 kb on + strand, within Psyr_0102 at 114.029 kb on - strand, within Psyr_0102 at 114.029 kb on - strand, within Psyr_0102 at 114.029 kb on - strand, within Psyr_0102 at 114.086 kb on + strand, within Psyr_0102 at 114.086 kb on + strand, within Psyr_0102 at 114.087 kb on - strand, within Psyr_0102 at 114.119 kb on - strand, within Psyr_0102 at 114.144 kb on + strand, within Psyr_0102 at 114.165 kb on - strand, within Psyr_0102 at 114.224 kb on + strand, within Psyr_0102 at 114.224 kb on + strand, within Psyr_0102 at 114.224 kb on + strand, within Psyr_0102 at 114.225 kb on - strand, within Psyr_0102 at 114.225 kb on - strand, within Psyr_0102 at 114.227 kb on + strand, within Psyr_0102 at 114.228 kb on - strand, within Psyr_0102 at 114.228 kb on - strand, within Psyr_0102 at 114.246 kb on - strand, within Psyr_0102 at 114.269 kb on + strand, within Psyr_0102 at 114.269 kb on + strand, within Psyr_0102 at 114.270 kb on - strand, within Psyr_0102 at 114.311 kb on + strand, within Psyr_0102 at 114.311 kb on + strand, within Psyr_0102 at 114.311 kb on + strand, within Psyr_0102 at 114.360 kb on + strand, within Psyr_0102 at 114.360 kb on + strand, within Psyr_0102 at 114.360 kb on + strand, within Psyr_0102 at 114.360 kb on + strand, within Psyr_0102 at 114.360 kb on + strand, within Psyr_0102 at 114.360 kb on + strand, within Psyr_0102 at 114.360 kb on + strand, within Psyr_0102 at 114.360 kb on + strand, within Psyr_0102 at 114.361 kb on - strand, within Psyr_0102 at 114.361 kb on - strand, within Psyr_0102 at 114.361 kb on - strand, within Psyr_0102 at 114.361 kb on - strand, within Psyr_0102 at 114.390 kb on - strand, within Psyr_0102 at 114.396 kb on - strand, within Psyr_0102 at 114.409 kb on + strand, within Psyr_0102 at 114.410 kb on - strand, within Psyr_0102 at 114.413 kb on + strand, within Psyr_0102 at 114.413 kb on + strand, within Psyr_0102 at 114.414 kb on - strand, within Psyr_0102 at 114.414 kb on - strand, within Psyr_0102 at 114.561 kb on - strand, within Psyr_0103 at 114.625 kb on - strand, within Psyr_0103 at 114.658 kb on - strand, within Psyr_0103 at 114.717 kb on + strand, within Psyr_0103 at 114.717 kb on + strand, within Psyr_0103 at 114.717 kb on + strand, within Psyr_0103 at 114.718 kb on - strand, within Psyr_0103 at 114.718 kb on - strand, within Psyr_0103 at 114.718 kb on - strand, within Psyr_0103 at 114.718 kb on - strand, within Psyr_0103 at 114.718 kb on - strand, within Psyr_0103 at 114.757 kb on + strand, within Psyr_0103 at 114.774 kb on - strand, within Psyr_0103 at 114.808 kb on + strand, within Psyr_0103 at 114.808 kb on + strand, within Psyr_0103 at 114.809 kb on - strand, within Psyr_0103 at 114.909 kb on - strand at 114.935 kb on + strand at 114.992 kb on + strand at 114.993 kb on - strand at 114.996 kb on - strand at 115.014 kb on + strand at 115.015 kb on - strand at 115.015 kb on - strand at 115.015 kb on - strand at 115.015 kb on - strand at 115.015 kb on - strand at 115.065 kb on + strand at 115.066 kb on - strand at 115.071 kb on + strand at 115.071 kb on + strand at 115.071 kb on + strand at 115.072 kb on - strand at 115.072 kb on - strand at 115.072 kb on - strand at 115.072 kb on - strand at 115.072 kb on - strand at 115.072 kb on - strand at 115.072 kb on - strand at 115.072 kb on - strand at 115.072 kb on - strand at 115.080 kb on + strand at 115.081 kb on - strand at 115.150 kb on + strand at 115.168 kb on + strand at 115.168 kb on + strand at 115.169 kb on - strand at 115.169 kb on - strand at 115.204 kb on + strand at 115.204 kb on + strand at 115.240 kb on + strand at 115.241 kb on - strand at 115.241 kb on - strand at 115.273 kb on - strand at 115.321 kb on + strand at 115.322 kb on - strand at 115.322 kb on - strand at 115.353 kb on + strand at 115.353 kb on + strand at 115.354 kb on - strand at 115.354 kb on - strand at 115.371 kb on - strand at 115.382 kb on + strand at 115.382 kb on + strand at 115.383 kb on - strand at 115.383 kb on - strand at 115.383 kb on - strand at 115.418 kb on - strand at 115.418 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Nalidixic 12.5 ug/ml remove 112,931 - +0.7 112,968 + +0.1 112,968 + +0.4 112,968 + -0.4 112,968 + +0.6 112,968 + -0.1 112,968 + +1.4 112,968 + -1.5 112,968 + -1.2 112,968 + -0.6 112,968 + -0.4 112,968 + +0.5 112,968 + -0.9 112,968 + -0.2 112,969 - -0.2 112,969 - -0.4 112,969 - -1.2 112,969 - -0.6 112,969 - +0.4 112,969 - -1.6 112,969 - +1.0 112,969 - +0.9 112,969 - -0.9 112,969 - -0.7 112,994 + -1.9 112,994 + -1.0 112,995 - -0.1 112,996 + +0.2 112,996 + -2.3 112,996 + +1.1 112,997 - -0.4 112,997 - -0.7 113,070 + Psyr_0101 0.17 +0.7 113,070 + Psyr_0101 0.17 +0.6 113,071 - Psyr_0101 0.17 -0.4 113,071 - Psyr_0101 0.17 +0.6 113,072 + Psyr_0101 0.17 +1.1 113,072 + Psyr_0101 0.17 -0.2 113,072 + Psyr_0101 0.17 +0.8 113,072 + Psyr_0101 0.17 +1.6 113,072 + Psyr_0101 0.17 -0.1 113,072 + Psyr_0101 0.17 -0.5 113,072 + Psyr_0101 0.17 -1.1 113,073 - Psyr_0101 0.17 +0.2 113,073 - Psyr_0101 0.17 -0.6 113,073 - Psyr_0101 0.17 +0.9 113,073 - Psyr_0101 0.17 -0.6 113,073 - Psyr_0101 0.17 +0.1 113,073 - Psyr_0101 0.17 -0.0 113,073 - Psyr_0101 0.17 -0.9 113,073 - Psyr_0101 0.17 +2.6 113,093 + Psyr_0101 0.21 -1.1 113,093 + Psyr_0101 0.21 -0.3 113,094 - Psyr_0101 0.21 -0.1 113,131 + Psyr_0101 0.29 +0.3 113,131 + Psyr_0101 0.29 -0.1 113,131 + Psyr_0101 0.29 -0.8 113,131 + Psyr_0101 0.29 +0.3 113,132 - Psyr_0101 0.29 -0.6 113,166 + Psyr_0101 0.36 +1.1 113,166 + Psyr_0101 0.36 -1.1 113,185 + Psyr_0101 0.40 -1.4 113,185 + Psyr_0101 0.40 -1.3 113,186 - Psyr_0101 0.40 -0.4 113,186 - Psyr_0101 0.40 -0.4 113,196 - Psyr_0101 0.42 -0.9 113,325 + Psyr_0101 0.67 -1.4 113,326 - +0.1 113,392 + Psyr_0101 0.81 -0.4 113,392 + Psyr_0101 0.81 -0.2 113,472 - -0.2 113,472 - -0.4 113,472 - -0.9 113,493 + +0.7 113,516 - -1.5 113,523 - -0.8 113,534 + -0.2 113,534 + -0.0 113,535 - -0.2 113,535 - +0.1 113,535 - +0.6 113,654 - -0.4 113,735 + -1.0 113,736 - -1.0 113,737 + -0.4 113,737 + -1.6 113,737 + -0.9 113,737 + -0.6 113,737 + -0.7 113,737 + +0.4 113,737 + +0.2 113,737 + -2.8 113,738 - +0.2 113,738 - -1.3 113,738 - -0.4 113,751 + -2.0 113,751 + -0.3 113,755 + -1.0 113,755 + -0.6 113,755 + -2.4 113,755 + -0.9 113,755 + -0.2 113,755 + +1.2 113,755 + +0.6 113,755 + -1.6 113,755 + -0.8 113,755 + +0.3 113,755 + -2.5 113,755 + +0.0 113,755 + +0.1 113,755 + -1.3 113,755 + -0.8 113,756 - -0.2 113,756 - -0.8 113,756 - -1.2 113,756 - -0.1 113,756 - -0.4 113,756 - -0.8 113,756 - -0.4 113,756 - -1.4 113,756 - +0.0 113,756 - -0.2 113,756 - -0.7 113,793 + -0.2 113,793 + -0.5 113,793 + -0.8 113,794 - +1.0 113,804 - +1.0 113,875 + -2.1 113,875 + -1.3 113,876 - -3.3 113,876 - -0.7 113,876 - -0.2 113,876 - -1.9 113,876 - -0.6 113,876 - -1.3 113,963 + -2.6 113,964 - -1.0 114,021 + Psyr_0102 0.19 +1.8 114,021 + Psyr_0102 0.19 -1.6 114,021 + Psyr_0102 0.19 +0.3 114,021 + Psyr_0102 0.19 +1.0 114,021 + Psyr_0102 0.19 -0.8 114,021 + Psyr_0102 0.19 -2.0 114,021 + Psyr_0102 0.19 -0.7 114,022 - Psyr_0102 0.19 +0.2 114,022 - Psyr_0102 0.19 -1.0 114,022 - Psyr_0102 0.19 +0.9 114,022 - Psyr_0102 0.19 +0.2 114,022 - Psyr_0102 0.19 -0.2 114,022 - Psyr_0102 0.19 -0.4 114,024 + Psyr_0102 0.20 -0.8 114,025 - Psyr_0102 0.20 -0.2 114,025 - Psyr_0102 0.20 +0.6 114,028 + Psyr_0102 0.20 -1.6 114,028 + Psyr_0102 0.20 -0.8 114,029 - Psyr_0102 0.21 -0.3 114,029 - Psyr_0102 0.21 -0.6 114,029 - Psyr_0102 0.21 -1.0 114,086 + Psyr_0102 0.30 -0.6 114,086 + Psyr_0102 0.30 +0.3 114,087 - Psyr_0102 0.30 -0.9 114,119 - Psyr_0102 0.36 -0.2 114,144 + Psyr_0102 0.40 -2.6 114,165 - Psyr_0102 0.43 -0.9 114,224 + Psyr_0102 0.53 -0.0 114,224 + Psyr_0102 0.53 +0.3 114,224 + Psyr_0102 0.53 -0.4 114,225 - Psyr_0102 0.53 +0.3 114,225 - Psyr_0102 0.53 +0.1 114,227 + Psyr_0102 0.54 -1.6 114,228 - Psyr_0102 0.54 -0.3 114,228 - Psyr_0102 0.54 -0.2 114,246 - Psyr_0102 0.57 -1.0 114,269 + Psyr_0102 0.61 +0.6 114,269 + Psyr_0102 0.61 +0.2 114,270 - Psyr_0102 0.61 -0.7 114,311 + Psyr_0102 0.68 -1.0 114,311 + Psyr_0102 0.68 -1.6 114,311 + Psyr_0102 0.68 -1.3 114,360 + Psyr_0102 0.76 -2.0 114,360 + Psyr_0102 0.76 +1.2 114,360 + Psyr_0102 0.76 -0.1 114,360 + Psyr_0102 0.76 +0.4 114,360 + Psyr_0102 0.76 -0.0 114,360 + Psyr_0102 0.76 -0.9 114,360 + Psyr_0102 0.76 -0.6 114,360 + Psyr_0102 0.76 -1.0 114,361 - Psyr_0102 0.76 +1.3 114,361 - Psyr_0102 0.76 -0.4 114,361 - Psyr_0102 0.76 -0.8 114,361 - Psyr_0102 0.76 +1.3 114,390 - Psyr_0102 0.81 -0.4 114,396 - Psyr_0102 0.82 -1.3 114,409 + Psyr_0102 0.84 -1.1 114,410 - Psyr_0102 0.84 -2.3 114,413 + Psyr_0102 0.85 +0.3 114,413 + Psyr_0102 0.85 -0.1 114,414 - Psyr_0102 0.85 -0.4 114,414 - Psyr_0102 0.85 -0.9 114,561 - Psyr_0103 0.12 +0.6 114,625 - Psyr_0103 0.29 -3.0 114,658 - Psyr_0103 0.38 -3.5 114,717 + Psyr_0103 0.54 -1.1 114,717 + Psyr_0103 0.54 +0.3 114,717 + Psyr_0103 0.54 +0.4 114,718 - Psyr_0103 0.54 -0.1 114,718 - Psyr_0103 0.54 -0.6 114,718 - Psyr_0103 0.54 -1.4 114,718 - Psyr_0103 0.54 -3.8 114,718 - Psyr_0103 0.54 -1.6 114,757 + Psyr_0103 0.64 +0.3 114,774 - Psyr_0103 0.69 -0.8 114,808 + Psyr_0103 0.78 +0.1 114,808 + Psyr_0103 0.78 -0.3 114,809 - Psyr_0103 0.78 -0.9 114,909 - -0.3 114,935 + +0.0 114,992 + +1.1 114,993 - -2.7 114,996 - -1.0 115,014 + -0.4 115,015 - -1.9 115,015 - -0.1 115,015 - -1.4 115,015 - +0.8 115,015 - -0.0 115,065 + +1.1 115,066 - -1.4 115,071 + +0.4 115,071 + +1.9 115,071 + -0.7 115,072 - -0.4 115,072 - -1.4 115,072 - +0.1 115,072 - -0.4 115,072 - -1.2 115,072 - -2.6 115,072 - -1.0 115,072 - -1.2 115,072 - -1.7 115,080 + -0.6 115,081 - -0.4 115,150 + +0.0 115,168 + -0.6 115,168 + +0.3 115,169 - -0.8 115,169 - -0.4 115,204 + +0.3 115,204 + -0.4 115,240 + -0.3 115,241 - -0.7 115,241 - +1.1 115,273 - -1.0 115,321 + -0.4 115,322 - +1.0 115,322 - -1.0 115,353 + -1.8 115,353 + -0.0 115,354 - +1.1 115,354 - -2.5 115,371 - -0.0 115,382 + -1.6 115,382 + +0.1 115,383 - -0.0 115,383 - -0.9 115,383 - -2.0 115,418 - -1.4 115,418 - -0.9
Or see this region's nucleotide sequence