Experiment: Carnitine Hydrochloride (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1304 and Pf6N2E2_1305 are separated by 171 nucleotides Pf6N2E2_1305 and Pf6N2E2_1306 are separated by 83 nucleotides Pf6N2E2_1306 and Pf6N2E2_1307 are separated by 186 nucleotides Pf6N2E2_1307 and Pf6N2E2_1308 overlap by 4 nucleotides
Pf6N2E2_1304 - Cell division transporter, ATP-binding protein FtsE (TC 3.A.5.1.1), at 1,539,474 to 1,539,722
_1304
Pf6N2E2_1305 - Homoprotocatechuate degradative operon repressor, at 1,539,894 to 1,540,295
_1305
Pf6N2E2_1306 - Transcriptional activator of 4-hydroxyphenylacetate 3-monooxygenase operon, XylS/AraC family, at 1,540,379 to 1,541,284
_1306
Pf6N2E2_1307 - 5-carboxymethyl-2-oxo-hex-3- ene-1,7-dioate decarboxylase (EC 4.1.1.68) / 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (EC 5.3.3.-), at 1,541,471 to 1,542,148
_1307
Pf6N2E2_1308 - 5-carboxymethyl-2-oxo-hex-3- ene-1,7-dioate decarboxylase (EC 4.1.1.68) / 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (EC 5.3.3.-), at 1,542,145 to 1,542,915
_1308
Position (kb) 1540
1541
1542 Strain fitness (log2 ratio) -2
-1
0
1 at 1539.389 kb on + strand at 1539.389 kb on + strand at 1539.980 kb on - strand, within Pf6N2E2_1305 at 1540.113 kb on + strand, within Pf6N2E2_1305 at 1540.114 kb on - strand, within Pf6N2E2_1305 at 1540.114 kb on - strand, within Pf6N2E2_1305 at 1540.350 kb on + strand at 1540.350 kb on + strand at 1540.350 kb on + strand at 1540.350 kb on + strand at 1540.351 kb on - strand at 1540.360 kb on - strand at 1540.662 kb on + strand, within Pf6N2E2_1306 at 1540.748 kb on + strand, within Pf6N2E2_1306 at 1540.748 kb on + strand, within Pf6N2E2_1306 at 1540.749 kb on - strand, within Pf6N2E2_1306 at 1540.851 kb on + strand, within Pf6N2E2_1306 at 1541.079 kb on + strand, within Pf6N2E2_1306 at 1541.080 kb on - strand, within Pf6N2E2_1306 at 1541.080 kb on - strand, within Pf6N2E2_1306 at 1541.099 kb on + strand, within Pf6N2E2_1306 at 1541.100 kb on - strand, within Pf6N2E2_1306 at 1541.100 kb on - strand, within Pf6N2E2_1306 at 1541.124 kb on + strand, within Pf6N2E2_1306 at 1541.206 kb on - strand at 1541.206 kb on - strand at 1541.390 kb on + strand at 1541.407 kb on - strand at 1541.751 kb on - strand, within Pf6N2E2_1307 at 1541.801 kb on + strand, within Pf6N2E2_1307 at 1541.801 kb on + strand, within Pf6N2E2_1307 at 1541.801 kb on + strand, within Pf6N2E2_1307 at 1541.802 kb on - strand, within Pf6N2E2_1307 at 1541.802 kb on - strand, within Pf6N2E2_1307 at 1541.802 kb on - strand, within Pf6N2E2_1307 at 1541.802 kb on - strand, within Pf6N2E2_1307 at 1541.802 kb on - strand, within Pf6N2E2_1307 at 1541.802 kb on - strand, within Pf6N2E2_1307 at 1541.912 kb on + strand, within Pf6N2E2_1307 at 1541.976 kb on + strand, within Pf6N2E2_1307 at 1541.976 kb on + strand, within Pf6N2E2_1307 at 1541.976 kb on + strand, within Pf6N2E2_1307 at 1541.976 kb on + strand, within Pf6N2E2_1307 at 1541.976 kb on + strand, within Pf6N2E2_1307 at 1542.058 kb on - strand, within Pf6N2E2_1307 at 1542.112 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carnitine Hydrochloride (C) remove 1,539,389 + -0.3 1,539,389 + +0.2 1,539,980 - Pf6N2E2_1305 0.21 +0.7 1,540,113 + Pf6N2E2_1305 0.54 +0.8 1,540,114 - Pf6N2E2_1305 0.55 -1.8 1,540,114 - Pf6N2E2_1305 0.55 +0.1 1,540,350 + -0.1 1,540,350 + +0.8 1,540,350 + -0.5 1,540,350 + -0.0 1,540,351 - -0.4 1,540,360 - +0.7 1,540,662 + Pf6N2E2_1306 0.31 -0.2 1,540,748 + Pf6N2E2_1306 0.41 -0.6 1,540,748 + Pf6N2E2_1306 0.41 +0.0 1,540,749 - Pf6N2E2_1306 0.41 -0.4 1,540,851 + Pf6N2E2_1306 0.52 -0.2 1,541,079 + Pf6N2E2_1306 0.77 -0.8 1,541,080 - Pf6N2E2_1306 0.77 +0.0 1,541,080 - Pf6N2E2_1306 0.77 +0.3 1,541,099 + Pf6N2E2_1306 0.79 -1.0 1,541,100 - Pf6N2E2_1306 0.80 +0.1 1,541,100 - Pf6N2E2_1306 0.80 +0.3 1,541,124 + Pf6N2E2_1306 0.82 -1.3 1,541,206 - +0.0 1,541,206 - +1.2 1,541,390 + +1.2 1,541,407 - +1.7 1,541,751 - Pf6N2E2_1307 0.41 -0.2 1,541,801 + Pf6N2E2_1307 0.49 +0.0 1,541,801 + Pf6N2E2_1307 0.49 +0.2 1,541,801 + Pf6N2E2_1307 0.49 -0.3 1,541,802 - Pf6N2E2_1307 0.49 -0.6 1,541,802 - Pf6N2E2_1307 0.49 -0.3 1,541,802 - Pf6N2E2_1307 0.49 +0.4 1,541,802 - Pf6N2E2_1307 0.49 +0.8 1,541,802 - Pf6N2E2_1307 0.49 +0.3 1,541,802 - Pf6N2E2_1307 0.49 +0.2 1,541,912 + Pf6N2E2_1307 0.65 -0.5 1,541,976 + Pf6N2E2_1307 0.74 -0.8 1,541,976 + Pf6N2E2_1307 0.74 -0.1 1,541,976 + Pf6N2E2_1307 0.74 +1.3 1,541,976 + Pf6N2E2_1307 0.74 +0.7 1,541,976 + Pf6N2E2_1307 0.74 +0.5 1,542,058 - Pf6N2E2_1307 0.87 +0.3 1,542,112 + +0.6
Or see this region's nucleotide sequence