Experiment: acetate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2805 and Pf1N1B4_2806 overlap by 8 nucleotides Pf1N1B4_2806 and Pf1N1B4_2807 overlap by 8 nucleotides Pf1N1B4_2807 and Pf1N1B4_2808 are separated by 192 nucleotides
Pf1N1B4_2805 - Isoleucyl-tRNA synthetase (EC 6.1.1.5), at 3,148,257 to 3,151,088
_2805
Pf1N1B4_2806 - Lipoprotein signal peptidase (EC 3.4.23.36), at 3,151,081 to 3,151,593
_2806
Pf1N1B4_2807 - FKBP-type peptidyl-prolyl cis-trans isomerase SlpA (EC 5.2.1.8), at 3,151,586 to 3,152,038
_2807
Pf1N1B4_2808 - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (EC 1.17.1.2), at 3,152,231 to 3,153,178
_2808
Position (kb)
3151
3152
3153 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3151.551 kb on + strand at 3151.551 kb on + strand at 3151.551 kb on + strand at 3151.551 kb on + strand at 3151.551 kb on + strand at 3151.599 kb on + strand at 3151.599 kb on + strand at 3151.599 kb on + strand at 3151.599 kb on + strand at 3151.599 kb on + strand at 3151.599 kb on + strand at 3151.600 kb on - strand at 3151.684 kb on + strand, within Pf1N1B4_2807 at 3151.684 kb on + strand, within Pf1N1B4_2807 at 3151.684 kb on + strand, within Pf1N1B4_2807 at 3151.685 kb on - strand, within Pf1N1B4_2807 at 3151.685 kb on - strand at 3151.861 kb on + strand, within Pf1N1B4_2807 at 3151.915 kb on + strand, within Pf1N1B4_2807 at 3151.990 kb on + strand, within Pf1N1B4_2807 at 3152.108 kb on + strand at 3152.108 kb on + strand at 3152.149 kb on + strand at 3152.197 kb on + strand at 3152.200 kb on + strand at 3152.200 kb on + strand at 3152.200 kb on + strand at 3152.200 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction acetate (C) remove 3,151,551 + +0.5 3,151,551 + +0.4 3,151,551 + -0.0 3,151,551 + -0.5 3,151,551 + +1.2 3,151,599 + -0.7 3,151,599 + -0.5 3,151,599 + +0.3 3,151,599 + +0.3 3,151,599 + +0.1 3,151,599 + -0.7 3,151,600 - -1.2 3,151,684 + Pf1N1B4_2807 0.22 -0.8 3,151,684 + Pf1N1B4_2807 0.22 -1.2 3,151,684 + Pf1N1B4_2807 0.22 -0.2 3,151,685 - Pf1N1B4_2807 0.22 +0.3 3,151,685 - -0.7 3,151,861 + Pf1N1B4_2807 0.61 -2.9 3,151,915 + Pf1N1B4_2807 0.73 -0.8 3,151,990 + Pf1N1B4_2807 0.89 +0.3 3,152,108 + -0.1 3,152,108 + +1.0 3,152,149 + +0.8 3,152,197 + -0.3 3,152,200 + +0.6 3,152,200 + -0.2 3,152,200 + -1.0 3,152,200 + +0.6
Or see this region's nucleotide sequence