Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 Vitamin & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS00820 and MMJJ_RS00825 are separated by 121 nucleotides MMJJ_RS00825 and MMJJ_RS00830 are separated by 24 nucleotides MMJJ_RS00830 and MMJJ_RS00835 are separated by 267 nucleotides
MMJJ_RS00820: MMJJ_RS00820 - MtaA/CmuA family methyltransferase, at 156,404 to 157,432
_RS00820
MMJJ_RS00825: MMJJ_RS00825 - uroporphyrinogen decarboxylase family protein, at 157,554 to 158,645
_RS00825
MMJJ_RS00830: MMJJ_RS00830 - corrinoid protein, at 158,670 to 159,329
_RS00830
MMJJ_RS00835: MMJJ_RS00835 - hypothetical protein, at 159,597 to 159,941
_RS00835
Position (kb) 157
158
159 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 156.617 kb on - strand, within MMJJ_RS00820 at 156.618 kb on + strand, within MMJJ_RS00820 at 156.618 kb on + strand, within MMJJ_RS00820 at 156.634 kb on - strand, within MMJJ_RS00820 at 156.634 kb on - strand, within MMJJ_RS00820 at 156.650 kb on - strand, within MMJJ_RS00820 at 156.651 kb on + strand, within MMJJ_RS00820 at 156.665 kb on - strand, within MMJJ_RS00820 at 156.674 kb on - strand, within MMJJ_RS00820 at 156.674 kb on - strand, within MMJJ_RS00820 at 156.766 kb on - strand, within MMJJ_RS00820 at 156.766 kb on - strand, within MMJJ_RS00820 at 156.766 kb on - strand, within MMJJ_RS00820 at 156.768 kb on - strand, within MMJJ_RS00820 at 156.769 kb on + strand, within MMJJ_RS00820 at 156.861 kb on + strand, within MMJJ_RS00820 at 156.937 kb on - strand, within MMJJ_RS00820 at 156.938 kb on + strand, within MMJJ_RS00820 at 156.947 kb on + strand, within MMJJ_RS00820 at 156.948 kb on - strand, within MMJJ_RS00820 at 156.948 kb on - strand, within MMJJ_RS00820 at 156.949 kb on + strand, within MMJJ_RS00820 at 156.951 kb on - strand, within MMJJ_RS00820 at 156.951 kb on - strand, within MMJJ_RS00820 at 156.952 kb on + strand, within MMJJ_RS00820 at 156.982 kb on - strand, within MMJJ_RS00820 at 156.986 kb on + strand, within MMJJ_RS00820 at 157.047 kb on + strand, within MMJJ_RS00820 at 157.139 kb on + strand, within MMJJ_RS00820 at 157.153 kb on - strand, within MMJJ_RS00820 at 157.154 kb on + strand, within MMJJ_RS00820 at 157.154 kb on + strand, within MMJJ_RS00820 at 157.156 kb on - strand, within MMJJ_RS00820 at 157.156 kb on - strand, within MMJJ_RS00820 at 157.199 kb on - strand, within MMJJ_RS00820 at 157.200 kb on + strand, within MMJJ_RS00820 at 157.224 kb on - strand, within MMJJ_RS00820 at 157.225 kb on + strand, within MMJJ_RS00820 at 157.261 kb on - strand, within MMJJ_RS00820 at 157.307 kb on - strand, within MMJJ_RS00820 at 157.308 kb on + strand, within MMJJ_RS00820 at 157.308 kb on + strand, within MMJJ_RS00820 at 157.336 kb on - strand at 157.336 kb on - strand at 157.364 kb on - strand at 157.365 kb on + strand at 157.365 kb on + strand at 157.464 kb on + strand at 157.481 kb on - strand at 157.520 kb on + strand at 157.555 kb on - strand at 157.556 kb on + strand at 157.565 kb on + strand at 157.581 kb on - strand at 157.585 kb on - strand at 157.586 kb on + strand at 157.659 kb on + strand at 157.662 kb on - strand at 157.663 kb on + strand at 157.663 kb on + strand at 157.663 kb on + strand at 157.668 kb on - strand, within MMJJ_RS00825 at 157.669 kb on + strand, within MMJJ_RS00825 at 157.669 kb on + strand, within MMJJ_RS00825 at 157.737 kb on + strand, within MMJJ_RS00825 at 157.779 kb on + strand, within MMJJ_RS00825 at 157.808 kb on - strand, within MMJJ_RS00825 at 157.879 kb on + strand, within MMJJ_RS00825 at 157.932 kb on - strand, within MMJJ_RS00825 at 157.932 kb on - strand, within MMJJ_RS00825 at 157.932 kb on - strand, within MMJJ_RS00825 at 157.934 kb on - strand, within MMJJ_RS00825 at 157.959 kb on - strand, within MMJJ_RS00825 at 157.980 kb on - strand, within MMJJ_RS00825 at 157.981 kb on + strand, within MMJJ_RS00825 at 157.981 kb on + strand, within MMJJ_RS00825 at 158.019 kb on - strand, within MMJJ_RS00825 at 158.019 kb on - strand, within MMJJ_RS00825 at 158.020 kb on + strand, within MMJJ_RS00825 at 158.027 kb on - strand, within MMJJ_RS00825 at 158.027 kb on - strand, within MMJJ_RS00825 at 158.027 kb on - strand, within MMJJ_RS00825 at 158.027 kb on - strand, within MMJJ_RS00825 at 158.027 kb on - strand, within MMJJ_RS00825 at 158.028 kb on + strand, within MMJJ_RS00825 at 158.028 kb on + strand, within MMJJ_RS00825 at 158.028 kb on + strand, within MMJJ_RS00825 at 158.051 kb on - strand, within MMJJ_RS00825 at 158.051 kb on - strand, within MMJJ_RS00825 at 158.052 kb on + strand, within MMJJ_RS00825 at 158.054 kb on - strand, within MMJJ_RS00825 at 158.106 kb on - strand, within MMJJ_RS00825 at 158.126 kb on - strand, within MMJJ_RS00825 at 158.223 kb on + strand, within MMJJ_RS00825 at 158.289 kb on + strand, within MMJJ_RS00825 at 158.316 kb on - strand, within MMJJ_RS00825 at 158.322 kb on + strand, within MMJJ_RS00825 at 158.344 kb on + strand, within MMJJ_RS00825 at 158.372 kb on - strand, within MMJJ_RS00825 at 158.465 kb on + strand, within MMJJ_RS00825 at 158.646 kb on + strand at 158.680 kb on + strand at 158.732 kb on - strand at 158.735 kb on + strand at 158.735 kb on + strand at 158.827 kb on + strand, within MMJJ_RS00830 at 158.945 kb on - strand, within MMJJ_RS00830 at 158.956 kb on - strand, within MMJJ_RS00830 at 158.995 kb on + strand, within MMJJ_RS00830 at 159.048 kb on + strand, within MMJJ_RS00830 at 159.076 kb on + strand, within MMJJ_RS00830 at 159.076 kb on + strand, within MMJJ_RS00830 at 159.106 kb on - strand, within MMJJ_RS00830 at 159.106 kb on - strand, within MMJJ_RS00830 at 159.106 kb on - strand, within MMJJ_RS00830 at 159.106 kb on - strand, within MMJJ_RS00830 at 159.107 kb on + strand, within MMJJ_RS00830 at 159.107 kb on + strand, within MMJJ_RS00830 at 159.107 kb on + strand, within MMJJ_RS00830 at 159.127 kb on + strand, within MMJJ_RS00830 at 159.130 kb on + strand, within MMJJ_RS00830 at 159.130 kb on + strand, within MMJJ_RS00830 at 159.238 kb on - strand, within MMJJ_RS00830 at 159.250 kb on + strand, within MMJJ_RS00830 at 159.276 kb on + strand at 159.303 kb on - strand at 159.304 kb on + strand at 159.304 kb on + strand at 159.304 kb on + strand at 159.354 kb on + strand at 159.475 kb on - strand at 159.490 kb on - strand at 159.538 kb on - strand at 159.538 kb on - strand at 159.538 kb on - strand at 159.539 kb on + strand at 159.539 kb on + strand at 159.539 kb on + strand at 159.541 kb on - strand at 159.541 kb on - strand at 159.542 kb on + strand at 159.542 kb on + strand at 159.542 kb on + strand at 159.544 kb on - strand at 159.544 kb on - strand at 159.545 kb on + strand at 159.545 kb on + strand at 159.545 kb on + strand at 159.545 kb on + strand at 159.545 kb on + strand at 159.545 kb on + strand at 159.546 kb on - strand at 159.547 kb on + strand at 159.547 kb on + strand at 159.547 kb on + strand at 159.547 kb on + strand at 159.549 kb on - strand at 159.549 kb on - strand at 159.550 kb on + strand at 159.550 kb on + strand at 159.550 kb on + strand at 159.551 kb on - strand at 159.551 kb on - strand at 159.551 kb on - strand at 159.559 kb on - strand at 159.559 kb on - strand at 159.560 kb on + strand at 159.560 kb on + strand at 159.560 kb on + strand at 159.561 kb on - strand at 159.576 kb on - strand at 159.581 kb on - strand at 159.581 kb on - strand at 159.581 kb on - strand at 159.581 kb on - strand at 159.582 kb on + strand at 159.586 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 Vitamin & Casamino Acid FREE remove 156,617 - MMJJ_RS00820 0.21 -1.1 156,618 + MMJJ_RS00820 0.21 +1.9 156,618 + MMJJ_RS00820 0.21 -0.8 156,634 - MMJJ_RS00820 0.22 -2.6 156,634 - MMJJ_RS00820 0.22 +1.9 156,650 - MMJJ_RS00820 0.24 -1.1 156,651 + MMJJ_RS00820 0.24 -0.8 156,665 - MMJJ_RS00820 0.25 +0.8 156,674 - MMJJ_RS00820 0.26 +1.6 156,674 - MMJJ_RS00820 0.26 +0.7 156,766 - MMJJ_RS00820 0.35 +0.5 156,766 - MMJJ_RS00820 0.35 -1.3 156,766 - MMJJ_RS00820 0.35 -1.2 156,768 - MMJJ_RS00820 0.35 +0.3 156,769 + MMJJ_RS00820 0.35 -0.4 156,861 + MMJJ_RS00820 0.44 +1.1 156,937 - MMJJ_RS00820 0.52 -1.8 156,938 + MMJJ_RS00820 0.52 +0.6 156,947 + MMJJ_RS00820 0.53 +0.4 156,948 - MMJJ_RS00820 0.53 -1.4 156,948 - MMJJ_RS00820 0.53 -1.5 156,949 + MMJJ_RS00820 0.53 -3.0 156,951 - MMJJ_RS00820 0.53 +0.5 156,951 - MMJJ_RS00820 0.53 -1.1 156,952 + MMJJ_RS00820 0.53 -1.1 156,982 - MMJJ_RS00820 0.56 -0.6 156,986 + MMJJ_RS00820 0.57 +0.4 157,047 + MMJJ_RS00820 0.62 +0.8 157,139 + MMJJ_RS00820 0.71 -0.0 157,153 - MMJJ_RS00820 0.73 -1.4 157,154 + MMJJ_RS00820 0.73 -1.0 157,154 + MMJJ_RS00820 0.73 -0.2 157,156 - MMJJ_RS00820 0.73 -1.4 157,156 - MMJJ_RS00820 0.73 +1.9 157,199 - MMJJ_RS00820 0.77 -0.1 157,200 + MMJJ_RS00820 0.77 -1.2 157,224 - MMJJ_RS00820 0.80 -2.6 157,225 + MMJJ_RS00820 0.80 -3.0 157,261 - MMJJ_RS00820 0.83 +0.2 157,307 - MMJJ_RS00820 0.88 +0.6 157,308 + MMJJ_RS00820 0.88 -2.2 157,308 + MMJJ_RS00820 0.88 +1.1 157,336 - +0.1 157,336 - -0.8 157,364 - -2.2 157,365 + -0.0 157,365 + -3.3 157,464 + +0.6 157,481 - -1.3 157,520 + -0.9 157,555 - +1.5 157,556 + -1.2 157,565 + +0.3 157,581 - +0.6 157,585 - -2.0 157,586 + -1.2 157,659 + +0.2 157,662 - +0.8 157,663 + +2.4 157,663 + +0.4 157,663 + -2.0 157,668 - MMJJ_RS00825 0.10 +0.7 157,669 + MMJJ_RS00825 0.11 -0.6 157,669 + MMJJ_RS00825 0.11 +1.4 157,737 + MMJJ_RS00825 0.17 -0.6 157,779 + MMJJ_RS00825 0.21 -0.6 157,808 - MMJJ_RS00825 0.23 +1.3 157,879 + MMJJ_RS00825 0.30 -1.0 157,932 - MMJJ_RS00825 0.35 -0.4 157,932 - MMJJ_RS00825 0.35 +0.4 157,932 - MMJJ_RS00825 0.35 -0.9 157,934 - MMJJ_RS00825 0.35 -0.2 157,959 - MMJJ_RS00825 0.37 +0.3 157,980 - MMJJ_RS00825 0.39 +1.2 157,981 + MMJJ_RS00825 0.39 +0.5 157,981 + MMJJ_RS00825 0.39 -2.7 158,019 - MMJJ_RS00825 0.43 -1.3 158,019 - MMJJ_RS00825 0.43 -1.1 158,020 + MMJJ_RS00825 0.43 +0.8 158,027 - MMJJ_RS00825 0.43 +0.4 158,027 - MMJJ_RS00825 0.43 -1.3 158,027 - MMJJ_RS00825 0.43 -1.0 158,027 - MMJJ_RS00825 0.43 -0.2 158,027 - MMJJ_RS00825 0.43 -0.7 158,028 + MMJJ_RS00825 0.43 +0.5 158,028 + MMJJ_RS00825 0.43 -1.4 158,028 + MMJJ_RS00825 0.43 +2.2 158,051 - MMJJ_RS00825 0.46 -1.4 158,051 - MMJJ_RS00825 0.46 +0.8 158,052 + MMJJ_RS00825 0.46 -1.0 158,054 - MMJJ_RS00825 0.46 -1.8 158,106 - MMJJ_RS00825 0.51 +0.5 158,126 - MMJJ_RS00825 0.52 +0.0 158,223 + MMJJ_RS00825 0.61 -2.2 158,289 + MMJJ_RS00825 0.67 -1.2 158,316 - MMJJ_RS00825 0.70 -1.6 158,322 + MMJJ_RS00825 0.70 +1.6 158,344 + MMJJ_RS00825 0.72 -0.8 158,372 - MMJJ_RS00825 0.75 -0.3 158,465 + MMJJ_RS00825 0.83 +0.1 158,646 + +0.8 158,680 + -1.6 158,732 - -1.7 158,735 + -1.8 158,735 + +1.6 158,827 + MMJJ_RS00830 0.24 -0.2 158,945 - MMJJ_RS00830 0.42 +1.1 158,956 - MMJJ_RS00830 0.43 +0.0 158,995 + MMJJ_RS00830 0.49 +0.0 159,048 + MMJJ_RS00830 0.57 +0.7 159,076 + MMJJ_RS00830 0.62 -0.9 159,076 + MMJJ_RS00830 0.62 -1.6 159,106 - MMJJ_RS00830 0.66 -1.4 159,106 - MMJJ_RS00830 0.66 +0.5 159,106 - MMJJ_RS00830 0.66 +2.0 159,106 - MMJJ_RS00830 0.66 -1.0 159,107 + MMJJ_RS00830 0.66 +0.1 159,107 + MMJJ_RS00830 0.66 -0.4 159,107 + MMJJ_RS00830 0.66 +1.7 159,127 + MMJJ_RS00830 0.69 +1.2 159,130 + MMJJ_RS00830 0.70 -1.9 159,130 + MMJJ_RS00830 0.70 -0.2 159,238 - MMJJ_RS00830 0.86 +0.0 159,250 + MMJJ_RS00830 0.88 -1.3 159,276 + -0.7 159,303 - +1.8 159,304 + +0.4 159,304 + -0.3 159,304 + +0.2 159,354 + +0.8 159,475 - +0.9 159,490 - +0.8 159,538 - +0.8 159,538 - +1.4 159,538 - -1.5 159,539 + -1.4 159,539 + +1.8 159,539 + -3.7 159,541 - +1.0 159,541 - -0.1 159,542 + +1.3 159,542 + -0.4 159,542 + +1.1 159,544 - -0.6 159,544 - +2.2 159,545 + -0.1 159,545 + -1.8 159,545 + +1.4 159,545 + +0.8 159,545 + +1.1 159,545 + +0.5 159,546 - +0.0 159,547 + +0.1 159,547 + -1.6 159,547 + -1.6 159,547 + +0.1 159,549 - +0.4 159,549 - -2.2 159,550 + -1.7 159,550 + -1.4 159,550 + -1.0 159,551 - +0.2 159,551 - -2.4 159,551 - +0.0 159,559 - +0.7 159,559 - +0.8 159,560 + -0.4 159,560 + -2.1 159,560 + +1.2 159,561 - +1.8 159,576 - +0.5 159,581 - -0.2 159,581 - -0.7 159,581 - -1.2 159,581 - -2.2 159,582 + +0.6 159,586 + -2.5
Or see this region's nucleotide sequence