Experiment: NAG 20 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS03255 and HMPREF1058_RS03250 are separated by 12 nucleotides HMPREF1058_RS03250 and HMPREF1058_RS03245 are separated by 219 nucleotides HMPREF1058_RS03245 and HMPREF1058_RS03240 are separated by 164 nucleotides
HMPREF1058_RS03255: HMPREF1058_RS03255 - glutathionylspermidine synthase family protein, at 320,149 to 321,273
_RS03255
HMPREF1058_RS03250: HMPREF1058_RS03250 - hypothetical protein, at 321,286 to 321,639
_RS03250
HMPREF1058_RS03245: HMPREF1058_RS03245 - thiamine phosphate synthase, at 321,859 to 322,467
_RS03245
HMPREF1058_RS03240: HMPREF1058_RS03240 - ribose-phosphate pyrophosphokinase, at 322,632 to 323,570
_RS03240
Position (kb) 321
322 Strain fitness (log2 ratio) -2
-1
0
1 at 320.306 kb on - strand, within HMPREF1058_RS03255 at 320.306 kb on - strand, within HMPREF1058_RS03255 at 320.306 kb on - strand, within HMPREF1058_RS03255 at 320.310 kb on - strand, within HMPREF1058_RS03255 at 320.323 kb on - strand, within HMPREF1058_RS03255 at 320.325 kb on - strand, within HMPREF1058_RS03255 at 320.346 kb on + strand, within HMPREF1058_RS03255 at 320.347 kb on - strand, within HMPREF1058_RS03255 at 320.351 kb on - strand, within HMPREF1058_RS03255 at 320.629 kb on + strand, within HMPREF1058_RS03255 at 320.629 kb on + strand, within HMPREF1058_RS03255 at 320.732 kb on + strand, within HMPREF1058_RS03255 at 320.839 kb on - strand, within HMPREF1058_RS03255 at 320.839 kb on - strand, within HMPREF1058_RS03255 at 320.923 kb on - strand, within HMPREF1058_RS03255 at 320.923 kb on - strand, within HMPREF1058_RS03255 at 320.968 kb on + strand, within HMPREF1058_RS03255 at 320.969 kb on - strand, within HMPREF1058_RS03255 at 320.969 kb on - strand, within HMPREF1058_RS03255 at 321.165 kb on + strand at 321.176 kb on + strand at 321.284 kb on + strand at 321.291 kb on + strand at 321.292 kb on - strand at 321.435 kb on - strand, within HMPREF1058_RS03250 at 321.437 kb on + strand, within HMPREF1058_RS03250 at 321.437 kb on + strand, within HMPREF1058_RS03250 at 321.437 kb on + strand, within HMPREF1058_RS03250 at 321.438 kb on - strand, within HMPREF1058_RS03250 at 321.438 kb on - strand, within HMPREF1058_RS03250 at 321.438 kb on - strand, within HMPREF1058_RS03250 at 321.440 kb on + strand, within HMPREF1058_RS03250 at 321.450 kb on + strand, within HMPREF1058_RS03250 at 321.494 kb on - strand, within HMPREF1058_RS03250 at 321.506 kb on + strand, within HMPREF1058_RS03250 at 321.507 kb on - strand, within HMPREF1058_RS03250 at 321.678 kb on - strand at 321.705 kb on + strand at 321.705 kb on + strand at 321.705 kb on + strand at 321.706 kb on - strand at 321.761 kb on - strand at 321.817 kb on - strand at 321.823 kb on - strand at 321.823 kb on - strand at 321.830 kb on + strand at 321.847 kb on + strand at 321.859 kb on - strand at 321.866 kb on + strand at 321.866 kb on + strand at 321.915 kb on + strand at 322.015 kb on + strand, within HMPREF1058_RS03245 at 322.117 kb on + strand, within HMPREF1058_RS03245 at 322.187 kb on - strand, within HMPREF1058_RS03245 at 322.301 kb on + strand, within HMPREF1058_RS03245 at 322.478 kb on + strand at 322.511 kb on - strand at 322.529 kb on - strand at 322.532 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction NAG 20 mM (C) remove 320,306 - HMPREF1058_RS03255 0.14 +1.0 320,306 - HMPREF1058_RS03255 0.14 +0.1 320,306 - HMPREF1058_RS03255 0.14 +0.5 320,310 - HMPREF1058_RS03255 0.14 +1.6 320,323 - HMPREF1058_RS03255 0.15 -0.2 320,325 - HMPREF1058_RS03255 0.16 +1.0 320,346 + HMPREF1058_RS03255 0.18 +0.6 320,347 - HMPREF1058_RS03255 0.18 +0.2 320,351 - HMPREF1058_RS03255 0.18 -0.1 320,629 + HMPREF1058_RS03255 0.43 -0.2 320,629 + HMPREF1058_RS03255 0.43 -0.5 320,732 + HMPREF1058_RS03255 0.52 -0.6 320,839 - HMPREF1058_RS03255 0.61 -0.4 320,839 - HMPREF1058_RS03255 0.61 +0.5 320,923 - HMPREF1058_RS03255 0.69 +0.7 320,923 - HMPREF1058_RS03255 0.69 -0.5 320,968 + HMPREF1058_RS03255 0.73 -0.0 320,969 - HMPREF1058_RS03255 0.73 +0.9 320,969 - HMPREF1058_RS03255 0.73 +0.6 321,165 + -0.2 321,176 + -1.0 321,284 + -0.1 321,291 + -1.6 321,292 - +0.2 321,435 - HMPREF1058_RS03250 0.42 -0.9 321,437 + HMPREF1058_RS03250 0.43 +0.1 321,437 + HMPREF1058_RS03250 0.43 -0.1 321,437 + HMPREF1058_RS03250 0.43 +1.0 321,438 - HMPREF1058_RS03250 0.43 -1.5 321,438 - HMPREF1058_RS03250 0.43 -0.6 321,438 - HMPREF1058_RS03250 0.43 +0.2 321,440 + HMPREF1058_RS03250 0.44 +0.7 321,450 + HMPREF1058_RS03250 0.46 -1.1 321,494 - HMPREF1058_RS03250 0.59 +0.3 321,506 + HMPREF1058_RS03250 0.62 +0.1 321,507 - HMPREF1058_RS03250 0.62 +0.6 321,678 - +0.2 321,705 + +0.6 321,705 + +0.3 321,705 + +0.4 321,706 - +0.2 321,761 - -0.5 321,817 - -0.2 321,823 - +0.2 321,823 - -0.7 321,830 + -0.8 321,847 + -0.7 321,859 - -1.1 321,866 + -1.6 321,866 + -1.2 321,915 + -0.2 322,015 + HMPREF1058_RS03245 0.26 -0.7 322,117 + HMPREF1058_RS03245 0.42 -2.3 322,187 - HMPREF1058_RS03245 0.54 -1.5 322,301 + HMPREF1058_RS03245 0.73 -1.1 322,478 + +0.5 322,511 - -0.7 322,529 - -1.0 322,532 - -0.7
Or see this region's nucleotide sequence