Experiment: D-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_651 and Pf6N2E2_652 are separated by 172 nucleotides Pf6N2E2_652 and Pf6N2E2_653 overlap by 1 nucleotides
Pf6N2E2_651 - General secretion pathway protein D, at 739,397 to 741,760
_651
Pf6N2E2_652 - General secretion pathway protein E, at 741,933 to 743,351
_652
Pf6N2E2_653 - General secretion pathway protein F, at 743,351 to 744,562
_653
Position (kb) 741
742
743
744 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 741.155 kb on + strand, within Pf6N2E2_651 at 741.155 kb on + strand, within Pf6N2E2_651 at 741.155 kb on + strand, within Pf6N2E2_651 at 741.156 kb on - strand, within Pf6N2E2_651 at 741.156 kb on - strand, within Pf6N2E2_651 at 741.156 kb on - strand, within Pf6N2E2_651 at 741.156 kb on - strand, within Pf6N2E2_651 at 741.278 kb on + strand, within Pf6N2E2_651 at 741.278 kb on + strand, within Pf6N2E2_651 at 741.278 kb on + strand, within Pf6N2E2_651 at 741.278 kb on + strand, within Pf6N2E2_651 at 741.279 kb on - strand, within Pf6N2E2_651 at 741.530 kb on + strand at 741.530 kb on + strand at 741.531 kb on - strand at 741.531 kb on - strand at 741.531 kb on - strand at 741.535 kb on + strand at 741.565 kb on + strand at 741.622 kb on + strand at 741.823 kb on + strand at 741.824 kb on - strand at 741.917 kb on + strand at 741.948 kb on + strand at 741.948 kb on + strand at 741.948 kb on + strand at 741.976 kb on + strand at 742.122 kb on + strand, within Pf6N2E2_652 at 742.123 kb on - strand, within Pf6N2E2_652 at 742.701 kb on + strand, within Pf6N2E2_652 at 742.701 kb on + strand, within Pf6N2E2_652 at 742.701 kb on + strand, within Pf6N2E2_652 at 742.702 kb on - strand, within Pf6N2E2_652 at 742.768 kb on - strand, within Pf6N2E2_652 at 742.768 kb on - strand, within Pf6N2E2_652 at 743.811 kb on - strand, within Pf6N2E2_653 at 743.915 kb on + strand, within Pf6N2E2_653 at 743.915 kb on + strand, within Pf6N2E2_653 at 743.915 kb on + strand, within Pf6N2E2_653 at 743.915 kb on + strand, within Pf6N2E2_653 at 743.916 kb on - strand, within Pf6N2E2_653 at 743.916 kb on - strand, within Pf6N2E2_653 at 744.065 kb on - strand, within Pf6N2E2_653 at 744.065 kb on - strand, within Pf6N2E2_653 at 744.068 kb on + strand, within Pf6N2E2_653 at 744.068 kb on + strand, within Pf6N2E2_653 at 744.068 kb on + strand, within Pf6N2E2_653 at 744.069 kb on - strand, within Pf6N2E2_653 at 744.069 kb on - strand, within Pf6N2E2_653 at 744.069 kb on - strand, within Pf6N2E2_653
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (C) remove 741,155 + Pf6N2E2_651 0.74 +0.3 741,155 + Pf6N2E2_651 0.74 +0.2 741,155 + Pf6N2E2_651 0.74 +1.1 741,156 - Pf6N2E2_651 0.74 +0.8 741,156 - Pf6N2E2_651 0.74 -1.6 741,156 - Pf6N2E2_651 0.74 -0.7 741,156 - Pf6N2E2_651 0.74 +1.1 741,278 + Pf6N2E2_651 0.80 -0.2 741,278 + Pf6N2E2_651 0.80 -1.2 741,278 + Pf6N2E2_651 0.80 +0.0 741,278 + Pf6N2E2_651 0.80 +1.2 741,279 - Pf6N2E2_651 0.80 -0.1 741,530 + +0.6 741,530 + +0.3 741,531 - -0.6 741,531 - +0.0 741,531 - +0.5 741,535 + -1.3 741,565 + +0.1 741,622 + -0.4 741,823 + +0.6 741,824 - +0.2 741,917 + +0.4 741,948 + +1.1 741,948 + -1.7 741,948 + +0.6 741,976 + -0.1 742,122 + Pf6N2E2_652 0.13 -1.7 742,123 - Pf6N2E2_652 0.13 -2.8 742,701 + Pf6N2E2_652 0.54 +0.1 742,701 + Pf6N2E2_652 0.54 -0.9 742,701 + Pf6N2E2_652 0.54 -1.1 742,702 - Pf6N2E2_652 0.54 -0.6 742,768 - Pf6N2E2_652 0.59 -1.4 742,768 - Pf6N2E2_652 0.59 +0.0 743,811 - Pf6N2E2_653 0.38 -0.4 743,915 + Pf6N2E2_653 0.47 +0.6 743,915 + Pf6N2E2_653 0.47 +0.4 743,915 + Pf6N2E2_653 0.47 +0.6 743,915 + Pf6N2E2_653 0.47 +0.4 743,916 - Pf6N2E2_653 0.47 -0.3 743,916 - Pf6N2E2_653 0.47 -1.1 744,065 - Pf6N2E2_653 0.59 -0.3 744,065 - Pf6N2E2_653 0.59 +1.7 744,068 + Pf6N2E2_653 0.59 +0.2 744,068 + Pf6N2E2_653 0.59 -0.3 744,068 + Pf6N2E2_653 0.59 +0.5 744,069 - Pf6N2E2_653 0.59 -1.0 744,069 - Pf6N2E2_653 0.59 +0.2 744,069 - Pf6N2E2_653 0.59 -0.2
Or see this region's nucleotide sequence