Experiment: D-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5087 and Pf6N2E2_5088 are separated by 70 nucleotides Pf6N2E2_5088 and Pf6N2E2_5089 are separated by 114 nucleotides Pf6N2E2_5089 and Pf6N2E2_5090 are separated by 167 nucleotides
Pf6N2E2_5087 - Transcription elongation factor GreB-related protein, at 5,870,123 to 5,870,614
_5087
Pf6N2E2_5088 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 5,870,685 to 5,871,041
_5088
Pf6N2E2_5089 - hypothetical protein, at 5,871,156 to 5,871,554
_5089
Pf6N2E2_5090 - hypothetical protein, at 5,871,722 to 5,872,024
_5090
Position (kb)
5871
5872 Strain fitness (log2 ratio)
-1
0
1
2 at 5870.529 kb on + strand, within Pf6N2E2_5087 at 5870.529 kb on + strand, within Pf6N2E2_5087 at 5870.529 kb on + strand, within Pf6N2E2_5087 at 5870.529 kb on + strand, within Pf6N2E2_5087 at 5870.530 kb on - strand, within Pf6N2E2_5087 at 5870.530 kb on - strand, within Pf6N2E2_5087 at 5870.530 kb on - strand, within Pf6N2E2_5087 at 5870.628 kb on - strand at 5870.686 kb on + strand at 5870.687 kb on - strand at 5870.857 kb on + strand, within Pf6N2E2_5088 at 5870.858 kb on - strand, within Pf6N2E2_5088 at 5870.933 kb on - strand, within Pf6N2E2_5088 at 5871.071 kb on - strand at 5871.071 kb on - strand at 5871.083 kb on + strand at 5871.083 kb on + strand at 5871.084 kb on - strand at 5871.084 kb on - strand at 5871.097 kb on + strand at 5871.097 kb on + strand at 5871.097 kb on + strand at 5871.098 kb on - strand at 5871.141 kb on + strand at 5871.141 kb on + strand at 5871.142 kb on - strand at 5871.238 kb on + strand, within Pf6N2E2_5089 at 5871.238 kb on + strand, within Pf6N2E2_5089 at 5871.238 kb on + strand, within Pf6N2E2_5089 at 5871.238 kb on + strand, within Pf6N2E2_5089 at 5871.238 kb on + strand, within Pf6N2E2_5089 at 5871.238 kb on + strand, within Pf6N2E2_5089 at 5871.238 kb on + strand, within Pf6N2E2_5089 at 5871.238 kb on + strand, within Pf6N2E2_5089 at 5871.239 kb on - strand, within Pf6N2E2_5089 at 5871.239 kb on - strand, within Pf6N2E2_5089 at 5871.239 kb on - strand, within Pf6N2E2_5089 at 5871.239 kb on - strand, within Pf6N2E2_5089 at 5871.239 kb on - strand, within Pf6N2E2_5089 at 5871.239 kb on - strand, within Pf6N2E2_5089 at 5871.239 kb on - strand, within Pf6N2E2_5089 at 5871.239 kb on - strand, within Pf6N2E2_5089 at 5871.239 kb on - strand, within Pf6N2E2_5089 at 5871.239 kb on - strand, within Pf6N2E2_5089 at 5871.239 kb on - strand, within Pf6N2E2_5089 at 5871.239 kb on - strand, within Pf6N2E2_5089 at 5871.247 kb on + strand, within Pf6N2E2_5089 at 5871.247 kb on + strand, within Pf6N2E2_5089 at 5871.247 kb on + strand, within Pf6N2E2_5089 at 5871.247 kb on + strand, within Pf6N2E2_5089 at 5871.247 kb on + strand, within Pf6N2E2_5089 at 5871.247 kb on + strand, within Pf6N2E2_5089 at 5871.248 kb on - strand, within Pf6N2E2_5089 at 5871.248 kb on - strand, within Pf6N2E2_5089 at 5871.248 kb on - strand, within Pf6N2E2_5089 at 5871.248 kb on - strand, within Pf6N2E2_5089 at 5871.248 kb on - strand, within Pf6N2E2_5089 at 5871.248 kb on - strand, within Pf6N2E2_5089 at 5871.248 kb on - strand, within Pf6N2E2_5089 at 5871.248 kb on - strand, within Pf6N2E2_5089 at 5871.248 kb on - strand, within Pf6N2E2_5089 at 5871.310 kb on + strand, within Pf6N2E2_5089 at 5871.310 kb on + strand, within Pf6N2E2_5089 at 5871.310 kb on + strand, within Pf6N2E2_5089 at 5871.311 kb on - strand, within Pf6N2E2_5089 at 5871.328 kb on + strand, within Pf6N2E2_5089 at 5871.472 kb on + strand, within Pf6N2E2_5089 at 5871.487 kb on + strand, within Pf6N2E2_5089 at 5871.488 kb on - strand, within Pf6N2E2_5089 at 5871.511 kb on + strand, within Pf6N2E2_5089 at 5871.512 kb on - strand, within Pf6N2E2_5089 at 5871.520 kb on + strand at 5871.520 kb on + strand at 5871.521 kb on - strand at 5871.521 kb on - strand at 5871.581 kb on - strand at 5871.684 kb on - strand at 5872.195 kb on + strand at 5872.371 kb on + strand at 5872.371 kb on + strand at 5872.404 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (C) remove 5,870,529 + Pf6N2E2_5087 0.83 -0.5 5,870,529 + Pf6N2E2_5087 0.83 +0.7 5,870,529 + Pf6N2E2_5087 0.83 +0.0 5,870,529 + Pf6N2E2_5087 0.83 -0.4 5,870,530 - Pf6N2E2_5087 0.83 +1.4 5,870,530 - Pf6N2E2_5087 0.83 +0.0 5,870,530 - Pf6N2E2_5087 0.83 -0.5 5,870,628 - -0.1 5,870,686 + +0.6 5,870,687 - +0.1 5,870,857 + Pf6N2E2_5088 0.48 +0.3 5,870,858 - Pf6N2E2_5088 0.48 +0.6 5,870,933 - Pf6N2E2_5088 0.69 -0.7 5,871,071 - +0.9 5,871,071 - +0.1 5,871,083 + +0.1 5,871,083 + +0.3 5,871,084 - +0.4 5,871,084 - +0.7 5,871,097 + +1.1 5,871,097 + +0.6 5,871,097 + -0.0 5,871,098 - -0.1 5,871,141 + -0.1 5,871,141 + +1.1 5,871,142 - +0.9 5,871,238 + Pf6N2E2_5089 0.21 +0.2 5,871,238 + Pf6N2E2_5089 0.21 -0.3 5,871,238 + Pf6N2E2_5089 0.21 -0.2 5,871,238 + Pf6N2E2_5089 0.21 -0.1 5,871,238 + Pf6N2E2_5089 0.21 +0.2 5,871,238 + Pf6N2E2_5089 0.21 +0.2 5,871,238 + Pf6N2E2_5089 0.21 -0.1 5,871,238 + Pf6N2E2_5089 0.21 -1.2 5,871,239 - Pf6N2E2_5089 0.21 -0.3 5,871,239 - Pf6N2E2_5089 0.21 -0.6 5,871,239 - Pf6N2E2_5089 0.21 +0.5 5,871,239 - Pf6N2E2_5089 0.21 -0.4 5,871,239 - Pf6N2E2_5089 0.21 +1.0 5,871,239 - Pf6N2E2_5089 0.21 +0.3 5,871,239 - Pf6N2E2_5089 0.21 +0.2 5,871,239 - Pf6N2E2_5089 0.21 +0.3 5,871,239 - Pf6N2E2_5089 0.21 +0.2 5,871,239 - Pf6N2E2_5089 0.21 +0.6 5,871,239 - Pf6N2E2_5089 0.21 +0.2 5,871,239 - Pf6N2E2_5089 0.21 -0.5 5,871,247 + Pf6N2E2_5089 0.23 +2.7 5,871,247 + Pf6N2E2_5089 0.23 +0.5 5,871,247 + Pf6N2E2_5089 0.23 +0.3 5,871,247 + Pf6N2E2_5089 0.23 +0.0 5,871,247 + Pf6N2E2_5089 0.23 -0.2 5,871,247 + Pf6N2E2_5089 0.23 -0.5 5,871,248 - Pf6N2E2_5089 0.23 +0.8 5,871,248 - Pf6N2E2_5089 0.23 +0.9 5,871,248 - Pf6N2E2_5089 0.23 +0.4 5,871,248 - Pf6N2E2_5089 0.23 -0.4 5,871,248 - Pf6N2E2_5089 0.23 +0.5 5,871,248 - Pf6N2E2_5089 0.23 +0.1 5,871,248 - Pf6N2E2_5089 0.23 +0.2 5,871,248 - Pf6N2E2_5089 0.23 +0.0 5,871,248 - Pf6N2E2_5089 0.23 +0.2 5,871,310 + Pf6N2E2_5089 0.39 +0.2 5,871,310 + Pf6N2E2_5089 0.39 -0.3 5,871,310 + Pf6N2E2_5089 0.39 +1.2 5,871,311 - Pf6N2E2_5089 0.39 +0.5 5,871,328 + Pf6N2E2_5089 0.43 +0.3 5,871,472 + Pf6N2E2_5089 0.79 +0.6 5,871,487 + Pf6N2E2_5089 0.83 +1.1 5,871,488 - Pf6N2E2_5089 0.83 +0.8 5,871,511 + Pf6N2E2_5089 0.89 +0.8 5,871,512 - Pf6N2E2_5089 0.89 +0.4 5,871,520 + +0.5 5,871,520 + -0.5 5,871,521 - +0.7 5,871,521 - +0.8 5,871,581 - +0.2 5,871,684 - +0.3 5,872,195 + +0.3 5,872,371 + -0.3 5,872,371 + -0.4 5,872,404 - -0.4
Or see this region's nucleotide sequence