Experiment: D-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1587 and Pf6N2E2_1588 are separated by 81 nucleotides Pf6N2E2_1588 and Pf6N2E2_1589 overlap by 11 nucleotides Pf6N2E2_1589 and Pf6N2E2_1590 are separated by 3 nucleotides
Pf6N2E2_1587 - Dihydroflavonol-4-reductase (EC 1.1.1.219), at 1,856,807 to 1,857,820
_1587
Pf6N2E2_1588 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 1,857,902 to 1,859,422
_1588
Pf6N2E2_1589 - Phosphoserine phosphatase (EC 3.1.3.3), at 1,859,412 to 1,860,023
_1589
Pf6N2E2_1590 - pentachlorophenol 4-monooxygenase, at 1,860,027 to 1,861,790
_1590
Position (kb)
1857
1858
1859
1860 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1857.047 kb on + strand, within Pf6N2E2_1587 at 1857.078 kb on - strand, within Pf6N2E2_1587 at 1857.252 kb on + strand, within Pf6N2E2_1587 at 1857.356 kb on - strand, within Pf6N2E2_1587 at 1857.408 kb on + strand, within Pf6N2E2_1587 at 1857.616 kb on + strand, within Pf6N2E2_1587 at 1857.617 kb on - strand, within Pf6N2E2_1587 at 1857.617 kb on - strand, within Pf6N2E2_1587 at 1857.621 kb on - strand, within Pf6N2E2_1587 at 1857.621 kb on - strand, within Pf6N2E2_1587 at 1857.621 kb on - strand, within Pf6N2E2_1587 at 1857.630 kb on - strand, within Pf6N2E2_1587 at 1857.800 kb on - strand at 1857.834 kb on + strand at 1857.834 kb on + strand at 1857.919 kb on + strand at 1857.920 kb on - strand at 1858.013 kb on - strand at 1858.060 kb on + strand, within Pf6N2E2_1588 at 1858.092 kb on + strand, within Pf6N2E2_1588 at 1858.092 kb on + strand, within Pf6N2E2_1588 at 1858.133 kb on + strand, within Pf6N2E2_1588 at 1858.133 kb on + strand, within Pf6N2E2_1588 at 1858.178 kb on + strand, within Pf6N2E2_1588 at 1858.179 kb on - strand at 1858.179 kb on - strand, within Pf6N2E2_1588 at 1858.479 kb on - strand, within Pf6N2E2_1588 at 1858.730 kb on - strand, within Pf6N2E2_1588 at 1858.788 kb on - strand, within Pf6N2E2_1588 at 1858.842 kb on + strand, within Pf6N2E2_1588 at 1858.916 kb on - strand, within Pf6N2E2_1588 at 1859.023 kb on - strand, within Pf6N2E2_1588 at 1859.023 kb on - strand, within Pf6N2E2_1588 at 1859.049 kb on - strand, within Pf6N2E2_1588 at 1859.252 kb on + strand, within Pf6N2E2_1588 at 1859.253 kb on - strand, within Pf6N2E2_1588 at 1859.351 kb on + strand at 1859.389 kb on + strand at 1859.390 kb on - strand at 1859.504 kb on + strand, within Pf6N2E2_1589 at 1859.619 kb on + strand, within Pf6N2E2_1589 at 1859.664 kb on + strand, within Pf6N2E2_1589 at 1859.665 kb on - strand, within Pf6N2E2_1589 at 1859.665 kb on - strand, within Pf6N2E2_1589 at 1859.665 kb on - strand, within Pf6N2E2_1589 at 1859.779 kb on - strand, within Pf6N2E2_1589 at 1859.779 kb on - strand, within Pf6N2E2_1589 at 1859.814 kb on + strand, within Pf6N2E2_1589 at 1859.815 kb on - strand, within Pf6N2E2_1589 at 1859.885 kb on - strand, within Pf6N2E2_1589 at 1859.946 kb on + strand, within Pf6N2E2_1589 at 1859.946 kb on + strand, within Pf6N2E2_1589 at 1859.946 kb on + strand, within Pf6N2E2_1589 at 1859.947 kb on - strand, within Pf6N2E2_1589 at 1859.947 kb on - strand, within Pf6N2E2_1589 at 1859.947 kb on - strand, within Pf6N2E2_1589 at 1860.006 kb on + strand at 1860.006 kb on + strand at 1860.007 kb on - strand at 1860.019 kb on - strand at 1860.327 kb on + strand, within Pf6N2E2_1590 at 1860.340 kb on - strand, within Pf6N2E2_1590
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (C) remove 1,857,047 + Pf6N2E2_1587 0.24 +1.1 1,857,078 - Pf6N2E2_1587 0.27 +0.4 1,857,252 + Pf6N2E2_1587 0.44 -0.0 1,857,356 - Pf6N2E2_1587 0.54 +1.2 1,857,408 + Pf6N2E2_1587 0.59 +1.2 1,857,616 + Pf6N2E2_1587 0.80 +0.8 1,857,617 - Pf6N2E2_1587 0.80 +0.8 1,857,617 - Pf6N2E2_1587 0.80 -0.2 1,857,621 - Pf6N2E2_1587 0.80 -0.9 1,857,621 - Pf6N2E2_1587 0.80 +1.1 1,857,621 - Pf6N2E2_1587 0.80 +0.6 1,857,630 - Pf6N2E2_1587 0.81 +0.8 1,857,800 - +1.0 1,857,834 + +0.8 1,857,834 + +0.5 1,857,919 + +0.2 1,857,920 - +0.0 1,858,013 - -0.6 1,858,060 + Pf6N2E2_1588 0.10 -0.3 1,858,092 + Pf6N2E2_1588 0.12 +1.6 1,858,092 + Pf6N2E2_1588 0.12 +0.6 1,858,133 + Pf6N2E2_1588 0.15 +0.2 1,858,133 + Pf6N2E2_1588 0.15 +1.2 1,858,178 + Pf6N2E2_1588 0.18 -3.4 1,858,179 - -0.4 1,858,179 - Pf6N2E2_1588 0.18 -0.7 1,858,479 - Pf6N2E2_1588 0.38 +0.8 1,858,730 - Pf6N2E2_1588 0.54 +0.9 1,858,788 - Pf6N2E2_1588 0.58 +0.6 1,858,842 + Pf6N2E2_1588 0.62 +1.5 1,858,916 - Pf6N2E2_1588 0.67 +0.4 1,859,023 - Pf6N2E2_1588 0.74 +0.6 1,859,023 - Pf6N2E2_1588 0.74 +0.8 1,859,049 - Pf6N2E2_1588 0.75 -0.1 1,859,252 + Pf6N2E2_1588 0.89 +0.8 1,859,253 - Pf6N2E2_1588 0.89 +2.1 1,859,351 + +1.0 1,859,389 + -1.6 1,859,390 - -0.1 1,859,504 + Pf6N2E2_1589 0.15 -0.4 1,859,619 + Pf6N2E2_1589 0.34 +1.0 1,859,664 + Pf6N2E2_1589 0.41 +0.1 1,859,665 - Pf6N2E2_1589 0.41 +0.1 1,859,665 - Pf6N2E2_1589 0.41 -0.1 1,859,665 - Pf6N2E2_1589 0.41 +0.4 1,859,779 - Pf6N2E2_1589 0.60 -0.0 1,859,779 - Pf6N2E2_1589 0.60 +0.7 1,859,814 + Pf6N2E2_1589 0.66 +0.6 1,859,815 - Pf6N2E2_1589 0.66 +0.4 1,859,885 - Pf6N2E2_1589 0.77 -1.2 1,859,946 + Pf6N2E2_1589 0.87 -0.8 1,859,946 + Pf6N2E2_1589 0.87 +1.0 1,859,946 + Pf6N2E2_1589 0.87 +0.2 1,859,947 - Pf6N2E2_1589 0.87 +2.3 1,859,947 - Pf6N2E2_1589 0.87 -0.3 1,859,947 - Pf6N2E2_1589 0.87 +1.3 1,860,006 + +0.1 1,860,006 + -0.1 1,860,007 - -0.1 1,860,019 - +0.5 1,860,327 + Pf6N2E2_1590 0.17 +0.5 1,860,340 - Pf6N2E2_1590 0.18 +1.1
Or see this region's nucleotide sequence