Experiment: D-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1586 and Pf6N2E2_1587 are separated by 565 nucleotides Pf6N2E2_1587 and Pf6N2E2_1588 are separated by 81 nucleotides
Pf6N2E2_1586 - Transcriptional regulator, TetR family, at 1,855,699 to 1,856,241
_1586
Pf6N2E2_1587 - Dihydroflavonol-4-reductase (EC 1.1.1.219), at 1,856,807 to 1,857,820
_1587
Pf6N2E2_1588 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 1,857,902 to 1,859,422
_1588
Position (kb)
1856
1857
1858 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1856.324 kb on - strand at 1856.324 kb on - strand at 1856.324 kb on - strand at 1856.327 kb on + strand at 1856.328 kb on - strand at 1856.328 kb on - strand at 1856.387 kb on + strand at 1856.387 kb on + strand at 1856.388 kb on - strand at 1856.388 kb on - strand at 1856.393 kb on - strand at 1856.416 kb on - strand at 1856.416 kb on - strand at 1856.443 kb on + strand at 1856.444 kb on - strand at 1856.444 kb on - strand at 1856.444 kb on - strand at 1856.454 kb on + strand at 1856.455 kb on - strand at 1856.851 kb on + strand at 1856.871 kb on + strand at 1856.889 kb on + strand at 1856.890 kb on - strand at 1856.890 kb on - strand at 1856.890 kb on - strand at 1857.047 kb on + strand, within Pf6N2E2_1587 at 1857.078 kb on - strand, within Pf6N2E2_1587 at 1857.252 kb on + strand, within Pf6N2E2_1587 at 1857.356 kb on - strand, within Pf6N2E2_1587 at 1857.408 kb on + strand, within Pf6N2E2_1587 at 1857.616 kb on + strand, within Pf6N2E2_1587 at 1857.617 kb on - strand, within Pf6N2E2_1587 at 1857.617 kb on - strand, within Pf6N2E2_1587 at 1857.621 kb on - strand, within Pf6N2E2_1587 at 1857.621 kb on - strand, within Pf6N2E2_1587 at 1857.621 kb on - strand, within Pf6N2E2_1587 at 1857.630 kb on - strand, within Pf6N2E2_1587 at 1857.800 kb on - strand at 1857.834 kb on + strand at 1857.834 kb on + strand at 1857.919 kb on + strand at 1857.920 kb on - strand at 1858.013 kb on - strand at 1858.060 kb on + strand, within Pf6N2E2_1588 at 1858.092 kb on + strand, within Pf6N2E2_1588 at 1858.092 kb on + strand, within Pf6N2E2_1588 at 1858.133 kb on + strand, within Pf6N2E2_1588 at 1858.133 kb on + strand, within Pf6N2E2_1588 at 1858.178 kb on + strand, within Pf6N2E2_1588 at 1858.179 kb on - strand at 1858.179 kb on - strand, within Pf6N2E2_1588 at 1858.479 kb on - strand, within Pf6N2E2_1588 at 1858.730 kb on - strand, within Pf6N2E2_1588 at 1858.788 kb on - strand, within Pf6N2E2_1588
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (C) remove 1,856,324 - +0.2 1,856,324 - +0.1 1,856,324 - +0.7 1,856,327 + -1.4 1,856,328 - +0.9 1,856,328 - -0.1 1,856,387 + +1.5 1,856,387 + +0.9 1,856,388 - +0.5 1,856,388 - +0.9 1,856,393 - +1.3 1,856,416 - +1.1 1,856,416 - +1.0 1,856,443 + -0.1 1,856,444 - +1.2 1,856,444 - +0.7 1,856,444 - +0.3 1,856,454 + +0.9 1,856,455 - +1.0 1,856,851 + +0.3 1,856,871 + -0.2 1,856,889 + +0.4 1,856,890 - +0.1 1,856,890 - +0.2 1,856,890 - +0.3 1,857,047 + Pf6N2E2_1587 0.24 +1.1 1,857,078 - Pf6N2E2_1587 0.27 +0.4 1,857,252 + Pf6N2E2_1587 0.44 -0.0 1,857,356 - Pf6N2E2_1587 0.54 +1.2 1,857,408 + Pf6N2E2_1587 0.59 +1.2 1,857,616 + Pf6N2E2_1587 0.80 +0.8 1,857,617 - Pf6N2E2_1587 0.80 +0.8 1,857,617 - Pf6N2E2_1587 0.80 -0.2 1,857,621 - Pf6N2E2_1587 0.80 -0.9 1,857,621 - Pf6N2E2_1587 0.80 +1.1 1,857,621 - Pf6N2E2_1587 0.80 +0.6 1,857,630 - Pf6N2E2_1587 0.81 +0.8 1,857,800 - +1.0 1,857,834 + +0.8 1,857,834 + +0.5 1,857,919 + +0.2 1,857,920 - +0.0 1,858,013 - -0.6 1,858,060 + Pf6N2E2_1588 0.10 -0.3 1,858,092 + Pf6N2E2_1588 0.12 +1.6 1,858,092 + Pf6N2E2_1588 0.12 +0.6 1,858,133 + Pf6N2E2_1588 0.15 +0.2 1,858,133 + Pf6N2E2_1588 0.15 +1.2 1,858,178 + Pf6N2E2_1588 0.18 -3.4 1,858,179 - -0.4 1,858,179 - Pf6N2E2_1588 0.18 -0.7 1,858,479 - Pf6N2E2_1588 0.38 +0.8 1,858,730 - Pf6N2E2_1588 0.54 +0.9 1,858,788 - Pf6N2E2_1588 0.58 +0.6
Or see this region's nucleotide sequence