Experiment: D-Gluconic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_676 and Pf1N1B4_677 are separated by 222 nucleotides Pf1N1B4_677 and Pf1N1B4_678 are separated by 257 nucleotides Pf1N1B4_678 and Pf1N1B4_679 are separated by 167 nucleotides
Pf1N1B4_676 - FIG00953991: hypothetical protein, at 773,494 to 773,853
_676
Pf1N1B4_677 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 774,076 to 774,390
_677
Pf1N1B4_678 - Pyruvate kinase (EC 2.7.1.40), at 774,648 to 776,099
_678
Pf1N1B4_679 - Enoyl-CoA hydratase (EC 4.2.1.17), at 776,267 to 777,016
_679
Position (kb) 774
775
776
777 Strain fitness (log2 ratio) -2
-1
0
1
2 at 773.698 kb on - strand, within Pf1N1B4_676 at 773.761 kb on - strand, within Pf1N1B4_676 at 773.813 kb on + strand, within Pf1N1B4_676 at 773.896 kb on + strand at 773.897 kb on - strand at 773.918 kb on - strand at 773.918 kb on - strand at 773.925 kb on + strand at 773.925 kb on + strand at 773.925 kb on + strand at 773.925 kb on + strand at 773.926 kb on - strand at 773.926 kb on - strand at 773.928 kb on - strand at 773.928 kb on - strand at 773.928 kb on - strand at 773.928 kb on - strand at 773.986 kb on + strand at 774.008 kb on + strand at 774.009 kb on - strand at 774.018 kb on + strand at 774.019 kb on - strand at 774.050 kb on + strand at 774.052 kb on + strand at 774.052 kb on + strand at 774.052 kb on + strand at 774.052 kb on + strand at 774.052 kb on + strand at 774.052 kb on + strand at 774.052 kb on + strand at 774.052 kb on + strand at 774.052 kb on + strand at 774.052 kb on + strand at 774.052 kb on + strand at 774.053 kb on - strand at 774.053 kb on - strand at 774.053 kb on - strand at 774.053 kb on - strand at 774.053 kb on - strand at 774.053 kb on - strand at 774.053 kb on - strand at 774.053 kb on - strand at 774.053 kb on - strand at 774.053 kb on - strand at 774.207 kb on - strand, within Pf1N1B4_677 at 774.207 kb on - strand, within Pf1N1B4_677 at 774.207 kb on - strand, within Pf1N1B4_677 at 774.226 kb on - strand, within Pf1N1B4_677 at 774.284 kb on + strand, within Pf1N1B4_677 at 774.284 kb on + strand, within Pf1N1B4_677 at 774.284 kb on + strand, within Pf1N1B4_677 at 774.284 kb on + strand, within Pf1N1B4_677 at 774.284 kb on + strand, within Pf1N1B4_677 at 774.284 kb on + strand, within Pf1N1B4_677 at 774.285 kb on - strand, within Pf1N1B4_677 at 774.285 kb on - strand, within Pf1N1B4_677 at 774.285 kb on - strand, within Pf1N1B4_677 at 774.285 kb on - strand, within Pf1N1B4_677 at 774.285 kb on - strand, within Pf1N1B4_677 at 774.285 kb on - strand, within Pf1N1B4_677 at 774.285 kb on - strand, within Pf1N1B4_677 at 774.285 kb on - strand, within Pf1N1B4_677 at 774.365 kb on + strand at 774.365 kb on + strand at 774.365 kb on + strand at 774.366 kb on - strand at 774.366 kb on - strand at 774.366 kb on - strand at 774.486 kb on - strand at 774.549 kb on + strand at 774.549 kb on + strand at 774.549 kb on + strand at 774.550 kb on - strand at 774.550 kb on - strand at 774.550 kb on - strand at 774.550 kb on - strand at 774.550 kb on - strand at 774.550 kb on - strand at 774.550 kb on - strand at 774.575 kb on - strand at 774.575 kb on - strand at 774.594 kb on + strand at 774.595 kb on - strand at 774.600 kb on + strand at 774.600 kb on + strand at 774.600 kb on + strand at 774.601 kb on - strand at 774.601 kb on - strand at 774.601 kb on - strand at 774.609 kb on + strand at 774.609 kb on + strand at 774.610 kb on - strand at 774.610 kb on - strand at 774.610 kb on - strand at 774.610 kb on - strand at 774.610 kb on - strand at 774.610 kb on - strand at 774.612 kb on + strand at 774.612 kb on + strand at 774.612 kb on + strand at 774.613 kb on - strand at 774.613 kb on - strand at 774.613 kb on - strand at 774.613 kb on - strand at 774.613 kb on - strand at 774.613 kb on - strand at 774.643 kb on + strand at 774.643 kb on + strand at 774.643 kb on + strand at 774.643 kb on + strand at 774.643 kb on + strand at 774.644 kb on - strand at 774.644 kb on - strand at 774.644 kb on - strand at 774.644 kb on - strand at 774.644 kb on - strand at 774.644 kb on - strand at 774.644 kb on - strand at 774.678 kb on - strand at 774.695 kb on + strand at 774.696 kb on - strand at 774.696 kb on - strand at 774.696 kb on - strand at 774.932 kb on + strand, within Pf1N1B4_678 at 774.932 kb on + strand, within Pf1N1B4_678 at 774.932 kb on + strand, within Pf1N1B4_678 at 774.933 kb on - strand, within Pf1N1B4_678 at 775.046 kb on + strand, within Pf1N1B4_678 at 775.058 kb on + strand, within Pf1N1B4_678 at 775.215 kb on + strand, within Pf1N1B4_678 at 775.216 kb on - strand, within Pf1N1B4_678 at 775.216 kb on - strand, within Pf1N1B4_678 at 775.257 kb on + strand, within Pf1N1B4_678 at 775.257 kb on + strand, within Pf1N1B4_678 at 775.552 kb on + strand, within Pf1N1B4_678 at 775.552 kb on + strand, within Pf1N1B4_678 at 775.553 kb on - strand, within Pf1N1B4_678 at 775.572 kb on + strand at 775.572 kb on + strand, within Pf1N1B4_678 at 775.572 kb on + strand, within Pf1N1B4_678 at 775.572 kb on + strand, within Pf1N1B4_678 at 775.573 kb on - strand, within Pf1N1B4_678 at 775.617 kb on + strand, within Pf1N1B4_678 at 775.752 kb on + strand, within Pf1N1B4_678 at 775.752 kb on + strand, within Pf1N1B4_678 at 775.752 kb on + strand, within Pf1N1B4_678 at 775.752 kb on + strand, within Pf1N1B4_678 at 775.752 kb on + strand, within Pf1N1B4_678 at 775.752 kb on + strand, within Pf1N1B4_678 at 775.752 kb on + strand, within Pf1N1B4_678 at 775.752 kb on + strand, within Pf1N1B4_678 at 775.753 kb on - strand, within Pf1N1B4_678 at 775.753 kb on - strand at 775.753 kb on - strand, within Pf1N1B4_678 at 775.753 kb on - strand, within Pf1N1B4_678 at 775.753 kb on - strand, within Pf1N1B4_678 at 775.753 kb on - strand, within Pf1N1B4_678 at 775.809 kb on + strand, within Pf1N1B4_678 at 775.854 kb on + strand, within Pf1N1B4_678 at 775.854 kb on + strand at 775.855 kb on - strand, within Pf1N1B4_678 at 775.855 kb on - strand, within Pf1N1B4_678 at 775.855 kb on - strand, within Pf1N1B4_678 at 775.911 kb on + strand, within Pf1N1B4_678 at 775.911 kb on + strand, within Pf1N1B4_678 at 775.911 kb on + strand, within Pf1N1B4_678 at 775.911 kb on + strand, within Pf1N1B4_678 at 775.912 kb on - strand at 775.912 kb on - strand, within Pf1N1B4_678 at 775.918 kb on + strand at 776.037 kb on + strand at 776.037 kb on + strand at 776.037 kb on + strand at 776.037 kb on + strand at 776.037 kb on + strand at 776.037 kb on + strand at 776.038 kb on - strand at 776.281 kb on + strand at 776.282 kb on - strand at 776.282 kb on - strand at 776.665 kb on - strand, within Pf1N1B4_679 at 776.773 kb on - strand, within Pf1N1B4_679 at 776.922 kb on + strand, within Pf1N1B4_679 at 776.922 kb on + strand, within Pf1N1B4_679 at 776.922 kb on + strand, within Pf1N1B4_679 at 776.923 kb on - strand, within Pf1N1B4_679 at 776.923 kb on - strand, within Pf1N1B4_679 at 776.923 kb on - strand, within Pf1N1B4_679 at 776.923 kb on - strand, within Pf1N1B4_679
Per-strain Table
Position Strand Gene LocusTag Fraction D-Gluconic Acid (C) remove 773,698 - Pf1N1B4_676 0.57 -0.3 773,761 - Pf1N1B4_676 0.74 -0.1 773,813 + Pf1N1B4_676 0.89 +1.2 773,896 + +1.8 773,897 - -0.3 773,918 - -0.3 773,918 - -1.0 773,925 + -1.6 773,925 + -1.6 773,925 + -0.0 773,925 + +1.0 773,926 - +0.1 773,926 - +0.3 773,928 - -1.1 773,928 - -1.2 773,928 - +1.0 773,928 - -1.2 773,986 + +1.2 774,008 + +0.4 774,009 - -0.8 774,018 + +1.8 774,019 - +0.6 774,050 + +0.6 774,052 + -0.8 774,052 + -1.6 774,052 + -0.0 774,052 + +1.7 774,052 + -0.1 774,052 + -0.3 774,052 + -0.6 774,052 + -0.1 774,052 + +0.2 774,052 + +1.2 774,052 + -1.0 774,053 - -0.1 774,053 - -0.2 774,053 - -1.2 774,053 - -1.3 774,053 - -0.8 774,053 - +0.6 774,053 - -0.3 774,053 - -1.3 774,053 - -0.3 774,053 - -0.9 774,207 - Pf1N1B4_677 0.42 -0.8 774,207 - Pf1N1B4_677 0.42 -0.5 774,207 - Pf1N1B4_677 0.42 -0.4 774,226 - Pf1N1B4_677 0.48 +1.7 774,284 + Pf1N1B4_677 0.66 +0.0 774,284 + Pf1N1B4_677 0.66 +0.8 774,284 + Pf1N1B4_677 0.66 -0.6 774,284 + Pf1N1B4_677 0.66 -0.8 774,284 + Pf1N1B4_677 0.66 -0.1 774,284 + Pf1N1B4_677 0.66 +0.2 774,285 - Pf1N1B4_677 0.66 -0.5 774,285 - Pf1N1B4_677 0.66 +0.2 774,285 - Pf1N1B4_677 0.66 +1.2 774,285 - Pf1N1B4_677 0.66 -1.9 774,285 - Pf1N1B4_677 0.66 -1.1 774,285 - Pf1N1B4_677 0.66 +1.2 774,285 - Pf1N1B4_677 0.66 -0.9 774,285 - Pf1N1B4_677 0.66 +1.0 774,365 + -0.8 774,365 + +0.7 774,365 + -0.3 774,366 - -0.2 774,366 - +0.9 774,366 - -0.5 774,486 - +1.2 774,549 + -0.3 774,549 + +0.6 774,549 + -0.3 774,550 - -1.6 774,550 - +0.2 774,550 - -0.3 774,550 - -1.3 774,550 - -1.1 774,550 - +0.8 774,550 - -1.2 774,575 - -0.2 774,575 - -0.6 774,594 + +0.2 774,595 - -0.1 774,600 + +1.0 774,600 + -0.5 774,600 + +0.2 774,601 - -0.7 774,601 - -0.1 774,601 - +0.2 774,609 + -0.9 774,609 + -0.6 774,610 - +0.7 774,610 - -1.9 774,610 - +0.2 774,610 - -2.5 774,610 - -0.0 774,610 - -0.2 774,612 + +0.4 774,612 + -0.3 774,612 + -2.3 774,613 - +1.2 774,613 - -0.3 774,613 - -1.1 774,613 - +1.2 774,613 - +0.2 774,613 - -0.6 774,643 + -0.3 774,643 + -0.3 774,643 + +0.8 774,643 + -0.5 774,643 + -1.6 774,644 - +0.2 774,644 - -2.1 774,644 - -1.1 774,644 - +1.8 774,644 - +0.2 774,644 - -1.6 774,644 - -1.3 774,678 - -0.6 774,695 + -0.7 774,696 - -1.9 774,696 - -1.5 774,696 - -1.0 774,932 + Pf1N1B4_678 0.20 -0.3 774,932 + Pf1N1B4_678 0.20 -0.6 774,932 + Pf1N1B4_678 0.20 -0.5 774,933 - Pf1N1B4_678 0.20 +0.2 775,046 + Pf1N1B4_678 0.27 -0.3 775,058 + Pf1N1B4_678 0.28 +1.0 775,215 + Pf1N1B4_678 0.39 -1.9 775,216 - Pf1N1B4_678 0.39 -2.2 775,216 - Pf1N1B4_678 0.39 -1.2 775,257 + Pf1N1B4_678 0.42 -0.2 775,257 + Pf1N1B4_678 0.42 -0.6 775,552 + Pf1N1B4_678 0.62 -0.7 775,552 + Pf1N1B4_678 0.62 -1.0 775,553 - Pf1N1B4_678 0.62 -0.9 775,572 + +0.5 775,572 + Pf1N1B4_678 0.64 -1.7 775,572 + Pf1N1B4_678 0.64 -1.8 775,572 + Pf1N1B4_678 0.64 -0.1 775,573 - Pf1N1B4_678 0.64 +0.1 775,617 + Pf1N1B4_678 0.67 -2.1 775,752 + Pf1N1B4_678 0.76 +0.8 775,752 + Pf1N1B4_678 0.76 -1.6 775,752 + Pf1N1B4_678 0.76 +1.2 775,752 + Pf1N1B4_678 0.76 +0.2 775,752 + Pf1N1B4_678 0.76 -2.1 775,752 + Pf1N1B4_678 0.76 +2.0 775,752 + Pf1N1B4_678 0.76 -1.6 775,752 + Pf1N1B4_678 0.76 -1.3 775,753 - Pf1N1B4_678 0.76 -0.6 775,753 - -0.1 775,753 - Pf1N1B4_678 0.76 +0.7 775,753 - Pf1N1B4_678 0.76 -0.5 775,753 - Pf1N1B4_678 0.76 -1.8 775,753 - Pf1N1B4_678 0.76 -1.7 775,809 + Pf1N1B4_678 0.80 -0.3 775,854 + Pf1N1B4_678 0.83 -2.5 775,854 + -0.8 775,855 - Pf1N1B4_678 0.83 -0.8 775,855 - Pf1N1B4_678 0.83 -1.9 775,855 - Pf1N1B4_678 0.83 +0.8 775,911 + Pf1N1B4_678 0.87 -1.3 775,911 + Pf1N1B4_678 0.87 -1.9 775,911 + Pf1N1B4_678 0.87 -2.3 775,911 + Pf1N1B4_678 0.87 +1.7 775,912 - +1.2 775,912 - Pf1N1B4_678 0.87 +0.2 775,918 + +1.2 776,037 + +0.7 776,037 + -1.2 776,037 + +0.3 776,037 + -0.5 776,037 + -1.5 776,037 + +0.8 776,038 - -1.9 776,281 + +1.0 776,282 - -0.8 776,282 - +1.0 776,665 - Pf1N1B4_679 0.53 +0.5 776,773 - Pf1N1B4_679 0.67 -0.3 776,922 + Pf1N1B4_679 0.87 -0.4 776,922 + Pf1N1B4_679 0.87 -0.3 776,922 + Pf1N1B4_679 0.87 +1.0 776,923 - Pf1N1B4_679 0.87 +0.3 776,923 - Pf1N1B4_679 0.87 -0.3 776,923 - Pf1N1B4_679 0.87 +0.2 776,923 - Pf1N1B4_679 0.87 +0.2
Or see this region's nucleotide sequence