Experiment: D-Gluconic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_6029 and Pf1N1B4_6030 are separated by 33 nucleotides Pf1N1B4_6030 and Pf1N1B4_6031 overlap by 4 nucleotides Pf1N1B4_6031 and Pf1N1B4_6032 are separated by 56 nucleotides
Pf1N1B4_6029 - uridine nucleosidase (EC 3.2.2.3) <small>(from data)</small>, at 493,499 to 494,527
_6029
Pf1N1B4_6030 - Ribose ABC transport system, high affinity permease RbsD (TC 3.A.1.2.1), at 494,561 to 494,965
_6030
Pf1N1B4_6031 - Ribokinase (EC 2.7.1.15) <small>(from data)</small>, at 494,962 to 495,879
_6031
Pf1N1B4_6032 - Ribose operon repressor, at 495,936 to 496,955
_6032
Position (kb) 494
495
496 Strain fitness (log2 ratio) -2
-1
0
1
2 at 494.118 kb on + strand, within Pf1N1B4_6029 at 494.119 kb on - strand, within Pf1N1B4_6029 at 494.119 kb on - strand, within Pf1N1B4_6029 at 494.209 kb on - strand, within Pf1N1B4_6029 at 494.209 kb on - strand at 494.209 kb on - strand, within Pf1N1B4_6029 at 494.351 kb on - strand, within Pf1N1B4_6029 at 494.517 kb on + strand at 494.518 kb on - strand at 494.518 kb on - strand at 494.563 kb on - strand at 494.563 kb on - strand at 494.563 kb on - strand at 494.571 kb on - strand at 494.571 kb on - strand at 494.599 kb on - strand at 494.792 kb on + strand, within Pf1N1B4_6030 at 494.792 kb on + strand, within Pf1N1B4_6030 at 494.792 kb on + strand, within Pf1N1B4_6030 at 494.792 kb on + strand, within Pf1N1B4_6030 at 494.793 kb on - strand, within Pf1N1B4_6030 at 494.793 kb on - strand, within Pf1N1B4_6030 at 494.806 kb on + strand, within Pf1N1B4_6030 at 494.807 kb on - strand, within Pf1N1B4_6030 at 494.807 kb on - strand, within Pf1N1B4_6030 at 494.974 kb on - strand at 494.983 kb on + strand at 494.983 kb on + strand at 494.983 kb on + strand at 494.984 kb on - strand at 494.984 kb on - strand at 494.984 kb on - strand at 495.493 kb on + strand, within Pf1N1B4_6031 at 495.494 kb on - strand, within Pf1N1B4_6031 at 495.681 kb on - strand, within Pf1N1B4_6031 at 495.857 kb on - strand at 495.857 kb on - strand at 495.857 kb on - strand at 495.860 kb on - strand at 495.879 kb on + strand at 495.879 kb on + strand at 495.879 kb on + strand at 495.879 kb on + strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.927 kb on - strand at 495.927 kb on - strand at 495.992 kb on + strand at 495.993 kb on - strand at 495.993 kb on - strand at 496.179 kb on - strand, within Pf1N1B4_6032 at 496.179 kb on - strand, within Pf1N1B4_6032 at 496.267 kb on - strand, within Pf1N1B4_6032 at 496.471 kb on + strand, within Pf1N1B4_6032 at 496.636 kb on + strand, within Pf1N1B4_6032 at 496.636 kb on + strand, within Pf1N1B4_6032 at 496.688 kb on - strand, within Pf1N1B4_6032 at 496.706 kb on - strand, within Pf1N1B4_6032 at 496.706 kb on - strand, within Pf1N1B4_6032 at 496.822 kb on + strand, within Pf1N1B4_6032 at 496.823 kb on - strand, within Pf1N1B4_6032 at 496.860 kb on + strand at 496.861 kb on - strand at 496.861 kb on - strand at 496.861 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Gluconic Acid (C) remove 494,118 + Pf1N1B4_6029 0.60 +0.9 494,119 - Pf1N1B4_6029 0.60 -1.7 494,119 - Pf1N1B4_6029 0.60 +0.0 494,209 - Pf1N1B4_6029 0.69 -0.1 494,209 - -1.4 494,209 - Pf1N1B4_6029 0.69 +0.4 494,351 - Pf1N1B4_6029 0.83 -0.1 494,517 + +0.5 494,518 - -2.5 494,518 - -1.4 494,563 - -0.8 494,563 - -0.9 494,563 - -0.1 494,571 - +0.1 494,571 - +0.9 494,599 - +0.9 494,792 + Pf1N1B4_6030 0.57 -0.1 494,792 + Pf1N1B4_6030 0.57 +1.1 494,792 + Pf1N1B4_6030 0.57 -0.0 494,792 + Pf1N1B4_6030 0.57 -0.1 494,793 - Pf1N1B4_6030 0.57 -0.1 494,793 - Pf1N1B4_6030 0.57 +1.9 494,806 + Pf1N1B4_6030 0.60 +1.4 494,807 - Pf1N1B4_6030 0.61 +0.7 494,807 - Pf1N1B4_6030 0.61 -1.5 494,974 - +0.1 494,983 + -0.5 494,983 + -0.9 494,983 + +1.2 494,984 - -0.9 494,984 - -1.0 494,984 - -0.1 495,493 + Pf1N1B4_6031 0.58 -0.6 495,494 - Pf1N1B4_6031 0.58 -0.9 495,681 - Pf1N1B4_6031 0.78 -1.1 495,857 - +0.2 495,857 - -0.7 495,857 - -0.2 495,860 - +1.3 495,879 + +0.4 495,879 + +0.9 495,879 + +1.5 495,879 + +0.1 495,880 - +0.1 495,880 - -0.7 495,880 - +1.9 495,880 - -0.7 495,880 - +0.3 495,880 - +0.3 495,880 - +0.1 495,880 - -0.1 495,880 - +0.3 495,880 - -1.5 495,880 - +0.1 495,880 - +1.3 495,880 - -0.3 495,880 - -0.8 495,880 - +0.6 495,880 - -0.1 495,880 - +0.5 495,880 - +0.4 495,880 - +0.7 495,880 - -1.1 495,927 - -0.1 495,927 - -0.5 495,992 + -0.1 495,993 - -1.1 495,993 - +0.5 496,179 - Pf1N1B4_6032 0.24 +1.6 496,179 - Pf1N1B4_6032 0.24 +1.4 496,267 - Pf1N1B4_6032 0.32 -0.1 496,471 + Pf1N1B4_6032 0.52 -0.4 496,636 + Pf1N1B4_6032 0.69 -2.5 496,636 + Pf1N1B4_6032 0.69 +0.5 496,688 - Pf1N1B4_6032 0.74 -0.8 496,706 - Pf1N1B4_6032 0.75 -0.8 496,706 - Pf1N1B4_6032 0.75 +0.7 496,822 + Pf1N1B4_6032 0.87 +1.7 496,823 - Pf1N1B4_6032 0.87 -0.1 496,860 + +1.3 496,861 - +0.0 496,861 - +2.5 496,861 - +0.4
Or see this region's nucleotide sequence