Experiment: D-Gluconic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_555 and Pf1N1B4_556 are separated by 166 nucleotides Pf1N1B4_556 and Pf1N1B4_557 are separated by 83 nucleotides Pf1N1B4_557 and Pf1N1B4_558 are separated by 215 nucleotides
Pf1N1B4_555 - Uncharacterized PLP-dependent aminotransferase YfdZ, at 651,653 to 652,870
_555
Pf1N1B4_556 - Gluconate permease, at 653,037 to 654,389
_556
Pf1N1B4_557 - Gluconokinase (EC 2.7.1.12), at 654,473 to 655,006
_557
Pf1N1B4_558 - Gluconate utilization system Gnt-I transcriptional repressor, at 655,222 to 656,253
_558
Position (kb) 653
654
655 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 652.061 kb on + strand, within Pf1N1B4_555 at 652.061 kb on + strand at 652.061 kb on + strand, within Pf1N1B4_555 at 652.062 kb on - strand, within Pf1N1B4_555 at 652.062 kb on - strand, within Pf1N1B4_555 at 652.062 kb on - strand, within Pf1N1B4_555 at 652.259 kb on + strand at 652.388 kb on + strand, within Pf1N1B4_555 at 652.388 kb on + strand, within Pf1N1B4_555 at 652.389 kb on - strand, within Pf1N1B4_555 at 652.466 kb on + strand at 652.466 kb on + strand, within Pf1N1B4_555 at 652.466 kb on + strand, within Pf1N1B4_555 at 652.466 kb on + strand, within Pf1N1B4_555 at 652.637 kb on + strand at 652.637 kb on + strand, within Pf1N1B4_555 at 652.637 kb on + strand, within Pf1N1B4_555 at 652.637 kb on + strand, within Pf1N1B4_555 at 652.637 kb on + strand, within Pf1N1B4_555 at 652.637 kb on + strand, within Pf1N1B4_555 at 652.637 kb on + strand, within Pf1N1B4_555 at 652.637 kb on + strand, within Pf1N1B4_555 at 652.637 kb on + strand, within Pf1N1B4_555 at 652.637 kb on + strand, within Pf1N1B4_555 at 652.637 kb on + strand, within Pf1N1B4_555 at 652.637 kb on + strand, within Pf1N1B4_555 at 652.664 kb on + strand, within Pf1N1B4_555 at 652.751 kb on + strand at 652.751 kb on + strand at 653.143 kb on + strand at 653.522 kb on - strand, within Pf1N1B4_556 at 653.532 kb on - strand, within Pf1N1B4_556 at 653.532 kb on - strand, within Pf1N1B4_556 at 653.532 kb on - strand, within Pf1N1B4_556 at 653.532 kb on - strand, within Pf1N1B4_556 at 653.532 kb on - strand, within Pf1N1B4_556 at 653.532 kb on - strand, within Pf1N1B4_556 at 653.533 kb on + strand, within Pf1N1B4_556 at 653.534 kb on - strand at 653.534 kb on - strand, within Pf1N1B4_556 at 653.534 kb on - strand, within Pf1N1B4_556 at 653.534 kb on - strand, within Pf1N1B4_556 at 653.534 kb on - strand, within Pf1N1B4_556 at 653.783 kb on + strand, within Pf1N1B4_556 at 653.784 kb on - strand, within Pf1N1B4_556 at 653.784 kb on - strand at 653.917 kb on - strand, within Pf1N1B4_556 at 654.326 kb on + strand at 654.327 kb on - strand at 654.354 kb on + strand at 654.923 kb on - strand, within Pf1N1B4_557 at 655.009 kb on - strand at 655.047 kb on - strand at 655.047 kb on - strand at 655.189 kb on + strand at 655.189 kb on + strand at 655.227 kb on + strand at 655.248 kb on + strand at 655.312 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Gluconic Acid (C) remove 652,061 + Pf1N1B4_555 0.33 -0.4 652,061 + -1.4 652,061 + Pf1N1B4_555 0.33 +0.2 652,062 - Pf1N1B4_555 0.34 +0.8 652,062 - Pf1N1B4_555 0.34 -1.3 652,062 - Pf1N1B4_555 0.34 +0.3 652,259 + -0.4 652,388 + Pf1N1B4_555 0.60 -0.1 652,388 + Pf1N1B4_555 0.60 +0.7 652,389 - Pf1N1B4_555 0.60 +0.0 652,466 + +0.6 652,466 + Pf1N1B4_555 0.67 -0.8 652,466 + Pf1N1B4_555 0.67 -0.5 652,466 + Pf1N1B4_555 0.67 +0.3 652,637 + +0.2 652,637 + Pf1N1B4_555 0.81 -0.6 652,637 + Pf1N1B4_555 0.81 -1.0 652,637 + Pf1N1B4_555 0.81 +1.2 652,637 + Pf1N1B4_555 0.81 +1.6 652,637 + Pf1N1B4_555 0.81 -0.3 652,637 + Pf1N1B4_555 0.81 +0.9 652,637 + Pf1N1B4_555 0.81 +0.1 652,637 + Pf1N1B4_555 0.81 -1.6 652,637 + Pf1N1B4_555 0.81 +0.2 652,637 + Pf1N1B4_555 0.81 +0.2 652,637 + Pf1N1B4_555 0.81 -0.2 652,664 + Pf1N1B4_555 0.83 -0.2 652,751 + -0.5 652,751 + +0.5 653,143 + -0.8 653,522 - Pf1N1B4_556 0.36 -0.1 653,532 - Pf1N1B4_556 0.37 -1.8 653,532 - Pf1N1B4_556 0.37 -0.8 653,532 - Pf1N1B4_556 0.37 -1.8 653,532 - Pf1N1B4_556 0.37 -0.8 653,532 - Pf1N1B4_556 0.37 -1.5 653,532 - Pf1N1B4_556 0.37 -2.5 653,533 + Pf1N1B4_556 0.37 -1.8 653,534 - -1.4 653,534 - Pf1N1B4_556 0.37 -1.6 653,534 - Pf1N1B4_556 0.37 -0.4 653,534 - Pf1N1B4_556 0.37 -2.0 653,534 - Pf1N1B4_556 0.37 -1.3 653,783 + Pf1N1B4_556 0.55 -1.6 653,784 - Pf1N1B4_556 0.55 -1.4 653,784 - -0.8 653,917 - Pf1N1B4_556 0.65 +1.2 654,326 + -1.8 654,327 - -1.4 654,354 + +0.2 654,923 - Pf1N1B4_557 0.84 -0.4 655,009 - -1.8 655,047 - -0.8 655,047 - +1.2 655,189 + -0.2 655,189 + -1.4 655,227 + -2.9 655,248 + -0.2 655,312 - -0.2
Or see this region's nucleotide sequence