Experiment: D-Gluconic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5448 and Pf1N1B4_5449 are separated by 312 nucleotides Pf1N1B4_5449 and Pf1N1B4_5450 overlap by 25 nucleotides
Pf1N1B4_5448 - hypothetical protein, at 194,385 to 194,507
_5448
Pf1N1B4_5449 - Error-prone repair protein UmuD, at 194,820 to 195,131
_5449
Pf1N1B4_5450 - Error-prone, lesion bypass DNA polymerase V (UmuC), at 195,107 to 196,402
_5450
Position (kb) 194
195 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 193.399 kb on + strand at 193.449 kb on + strand at 193.450 kb on - strand at 193.552 kb on - strand at 193.552 kb on - strand at 193.836 kb on + strand at 193.836 kb on + strand at 193.837 kb on - strand at 193.837 kb on - strand at 193.873 kb on - strand at 193.879 kb on + strand at 193.880 kb on - strand at 193.880 kb on - strand at 193.889 kb on - strand at 193.889 kb on - strand at 193.889 kb on - strand at 194.054 kb on + strand at 194.055 kb on - strand at 194.055 kb on - strand at 194.176 kb on + strand at 194.176 kb on + strand at 194.177 kb on - strand at 194.177 kb on - strand at 194.177 kb on - strand at 194.177 kb on - strand at 194.278 kb on - strand at 194.278 kb on - strand at 194.328 kb on + strand at 194.328 kb on + strand at 194.329 kb on - strand at 194.440 kb on + strand, within Pf1N1B4_5448 at 194.441 kb on - strand, within Pf1N1B4_5448 at 194.441 kb on - strand, within Pf1N1B4_5448 at 194.441 kb on - strand, within Pf1N1B4_5448 at 194.441 kb on - strand at 194.441 kb on - strand, within Pf1N1B4_5448 at 194.495 kb on + strand at 194.496 kb on - strand at 194.543 kb on - strand at 194.543 kb on - strand at 194.543 kb on - strand at 194.601 kb on + strand at 194.602 kb on - strand at 194.672 kb on - strand at 194.709 kb on - strand at 194.709 kb on - strand at 194.730 kb on + strand at 194.731 kb on - strand at 194.731 kb on - strand at 194.731 kb on - strand at 194.749 kb on + strand at 194.750 kb on - strand at 194.798 kb on + strand at 194.798 kb on + strand at 194.799 kb on - strand at 194.799 kb on - strand at 195.050 kb on + strand, within Pf1N1B4_5449 at 195.051 kb on - strand, within Pf1N1B4_5449 at 195.173 kb on + strand at 195.174 kb on - strand at 195.282 kb on + strand, within Pf1N1B4_5450 at 195.282 kb on + strand, within Pf1N1B4_5450 at 195.345 kb on + strand at 195.345 kb on + strand, within Pf1N1B4_5450 at 195.345 kb on + strand, within Pf1N1B4_5450 at 195.345 kb on + strand, within Pf1N1B4_5450 at 195.346 kb on - strand, within Pf1N1B4_5450 at 195.346 kb on - strand, within Pf1N1B4_5450 at 195.346 kb on - strand, within Pf1N1B4_5450 at 195.349 kb on - strand, within Pf1N1B4_5450 at 195.349 kb on - strand, within Pf1N1B4_5450 at 195.379 kb on + strand, within Pf1N1B4_5450 at 195.441 kb on + strand, within Pf1N1B4_5450 at 195.441 kb on + strand, within Pf1N1B4_5450 at 195.441 kb on + strand, within Pf1N1B4_5450 at 195.442 kb on - strand, within Pf1N1B4_5450 at 195.442 kb on - strand, within Pf1N1B4_5450 at 195.456 kb on + strand, within Pf1N1B4_5450 at 195.456 kb on + strand, within Pf1N1B4_5450 at 195.456 kb on + strand, within Pf1N1B4_5450 at 195.456 kb on + strand, within Pf1N1B4_5450 at 195.456 kb on + strand, within Pf1N1B4_5450 at 195.457 kb on - strand, within Pf1N1B4_5450 at 195.457 kb on - strand, within Pf1N1B4_5450 at 195.457 kb on - strand, within Pf1N1B4_5450
Per-strain Table
Position Strand Gene LocusTag Fraction D-Gluconic Acid (C) remove 193,399 + +0.7 193,449 + +1.0 193,450 - +0.5 193,552 - -0.7 193,552 - +0.8 193,836 + +1.4 193,836 + -1.3 193,837 - +0.2 193,837 - -0.0 193,873 - +1.2 193,879 + -1.7 193,880 - +2.4 193,880 - +3.6 193,889 - -1.2 193,889 - -0.8 193,889 - -0.0 194,054 + +1.8 194,055 - -0.5 194,055 - +1.2 194,176 + +0.2 194,176 + +0.4 194,177 - -0.9 194,177 - -0.6 194,177 - +0.1 194,177 - +0.7 194,278 - +0.1 194,278 - -2.0 194,328 + +1.1 194,328 + -1.1 194,329 - -0.0 194,440 + Pf1N1B4_5448 0.45 -1.5 194,441 - Pf1N1B4_5448 0.46 -0.3 194,441 - Pf1N1B4_5448 0.46 -0.5 194,441 - Pf1N1B4_5448 0.46 -1.0 194,441 - +2.0 194,441 - Pf1N1B4_5448 0.46 +1.2 194,495 + -1.0 194,496 - +2.0 194,543 - -0.5 194,543 - +1.0 194,543 - -0.0 194,601 + +0.4 194,602 - +1.4 194,672 - +1.6 194,709 - -0.8 194,709 - -0.5 194,730 + -0.2 194,731 - -1.8 194,731 - +0.2 194,731 - +1.0 194,749 + +0.6 194,750 - +1.2 194,798 + -0.0 194,798 + +0.7 194,799 - +1.2 194,799 - -1.5 195,050 + Pf1N1B4_5449 0.74 -0.0 195,051 - Pf1N1B4_5449 0.74 +0.8 195,173 + +1.4 195,174 - +0.8 195,282 + Pf1N1B4_5450 0.14 +0.3 195,282 + Pf1N1B4_5450 0.14 -1.9 195,345 + -0.0 195,345 + Pf1N1B4_5450 0.18 -0.5 195,345 + Pf1N1B4_5450 0.18 +0.2 195,345 + Pf1N1B4_5450 0.18 -0.6 195,346 - Pf1N1B4_5450 0.18 +0.6 195,346 - Pf1N1B4_5450 0.18 +0.1 195,346 - Pf1N1B4_5450 0.18 -0.2 195,349 - Pf1N1B4_5450 0.19 +0.2 195,349 - Pf1N1B4_5450 0.19 +1.5 195,379 + Pf1N1B4_5450 0.21 -0.3 195,441 + Pf1N1B4_5450 0.26 +0.8 195,441 + Pf1N1B4_5450 0.26 -0.9 195,441 + Pf1N1B4_5450 0.26 +0.2 195,442 - Pf1N1B4_5450 0.26 -1.0 195,442 - Pf1N1B4_5450 0.26 -0.3 195,456 + Pf1N1B4_5450 0.27 +1.5 195,456 + Pf1N1B4_5450 0.27 -0.0 195,456 + Pf1N1B4_5450 0.27 -0.0 195,456 + Pf1N1B4_5450 0.27 -0.5 195,456 + Pf1N1B4_5450 0.27 -1.4 195,457 - Pf1N1B4_5450 0.27 +0.2 195,457 - Pf1N1B4_5450 0.27 +0.4 195,457 - Pf1N1B4_5450 0.27 -0.6
Or see this region's nucleotide sequence