Experiment: D-Gluconic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5414 and Pf1N1B4_5415 are separated by 3 nucleotides Pf1N1B4_5415 and Pf1N1B4_5416 are separated by 95 nucleotides Pf1N1B4_5416 and Pf1N1B4_5417 are separated by 11 nucleotides
Pf1N1B4_5414 - IncF plasmid conjugative transfer pilus assembly protein TraC, at 152,475 to 155,366
_5414
Pf1N1B4_5415 - hypothetical protein, at 155,370 to 155,732
_5415
Pf1N1B4_5416 - hypothetical protein, at 155,828 to 156,166
_5416
Pf1N1B4_5417 - hypothetical protein, at 156,178 to 157,722
_5417
Position (kb)
155
156 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 154.370 kb on - strand at 154.492 kb on + strand, within Pf1N1B4_5414 at 154.917 kb on + strand, within Pf1N1B4_5414 at 155.050 kb on + strand, within Pf1N1B4_5414 at 155.158 kb on + strand at 155.343 kb on + strand at 155.343 kb on + strand at 155.344 kb on - strand at 155.344 kb on - strand at 155.344 kb on - strand at 155.414 kb on - strand, within Pf1N1B4_5415 at 155.432 kb on + strand at 155.449 kb on + strand, within Pf1N1B4_5415 at 155.511 kb on + strand, within Pf1N1B4_5415 at 155.524 kb on + strand, within Pf1N1B4_5415 at 155.524 kb on + strand, within Pf1N1B4_5415 at 155.524 kb on + strand, within Pf1N1B4_5415 at 155.524 kb on + strand, within Pf1N1B4_5415 at 155.524 kb on + strand, within Pf1N1B4_5415 at 155.549 kb on + strand, within Pf1N1B4_5415 at 155.549 kb on + strand, within Pf1N1B4_5415 at 155.628 kb on + strand, within Pf1N1B4_5415 at 155.628 kb on + strand, within Pf1N1B4_5415 at 155.629 kb on - strand, within Pf1N1B4_5415 at 155.804 kb on + strand at 155.805 kb on - strand at 155.805 kb on - strand at 155.805 kb on - strand at 155.825 kb on + strand at 155.825 kb on + strand at 155.826 kb on - strand at 155.826 kb on - strand at 155.829 kb on + strand at 155.830 kb on - strand at 155.862 kb on + strand, within Pf1N1B4_5416 at 155.862 kb on + strand, within Pf1N1B4_5416 at 155.862 kb on + strand, within Pf1N1B4_5416 at 155.862 kb on + strand, within Pf1N1B4_5416 at 155.862 kb on + strand, within Pf1N1B4_5416 at 155.862 kb on + strand, within Pf1N1B4_5416 at 155.862 kb on + strand, within Pf1N1B4_5416 at 155.863 kb on - strand at 155.930 kb on + strand, within Pf1N1B4_5416 at 155.931 kb on - strand, within Pf1N1B4_5416 at 155.958 kb on + strand, within Pf1N1B4_5416 at 155.958 kb on + strand, within Pf1N1B4_5416 at 155.958 kb on + strand, within Pf1N1B4_5416 at 155.958 kb on + strand, within Pf1N1B4_5416 at 155.959 kb on - strand, within Pf1N1B4_5416 at 155.959 kb on - strand, within Pf1N1B4_5416 at 156.051 kb on + strand, within Pf1N1B4_5416 at 156.051 kb on + strand, within Pf1N1B4_5416 at 156.051 kb on + strand, within Pf1N1B4_5416 at 156.064 kb on + strand, within Pf1N1B4_5416 at 156.064 kb on + strand, within Pf1N1B4_5416 at 156.064 kb on + strand, within Pf1N1B4_5416 at 156.065 kb on - strand, within Pf1N1B4_5416 at 156.065 kb on - strand at 156.065 kb on - strand, within Pf1N1B4_5416 at 156.065 kb on - strand, within Pf1N1B4_5416 at 156.065 kb on - strand, within Pf1N1B4_5416 at 156.148 kb on + strand at 156.180 kb on - strand at 156.367 kb on + strand, within Pf1N1B4_5417 at 156.367 kb on + strand at 156.367 kb on + strand, within Pf1N1B4_5417 at 156.368 kb on - strand, within Pf1N1B4_5417 at 156.393 kb on + strand, within Pf1N1B4_5417 at 156.394 kb on - strand, within Pf1N1B4_5417 at 156.405 kb on + strand at 156.476 kb on + strand, within Pf1N1B4_5417 at 156.476 kb on + strand, within Pf1N1B4_5417 at 156.545 kb on + strand, within Pf1N1B4_5417 at 156.545 kb on + strand, within Pf1N1B4_5417 at 156.545 kb on + strand, within Pf1N1B4_5417 at 156.546 kb on - strand, within Pf1N1B4_5417 at 156.581 kb on + strand, within Pf1N1B4_5417 at 156.581 kb on + strand, within Pf1N1B4_5417 at 156.581 kb on + strand, within Pf1N1B4_5417 at 156.582 kb on - strand, within Pf1N1B4_5417
Per-strain Table
Position Strand Gene LocusTag Fraction D-Gluconic Acid (C) remove 154,370 - +2.0 154,492 + Pf1N1B4_5414 0.70 -1.0 154,917 + Pf1N1B4_5414 0.84 +0.4 155,050 + Pf1N1B4_5414 0.89 -0.8 155,158 + -0.3 155,343 + -0.0 155,343 + -0.4 155,344 - -0.6 155,344 - +0.4 155,344 - +0.4 155,414 - Pf1N1B4_5415 0.12 +0.6 155,432 + +0.4 155,449 + Pf1N1B4_5415 0.22 -0.4 155,511 + Pf1N1B4_5415 0.39 -0.7 155,524 + Pf1N1B4_5415 0.42 +2.0 155,524 + Pf1N1B4_5415 0.42 +1.0 155,524 + Pf1N1B4_5415 0.42 -0.5 155,524 + Pf1N1B4_5415 0.42 -2.6 155,524 + Pf1N1B4_5415 0.42 -0.0 155,549 + Pf1N1B4_5415 0.49 +0.4 155,549 + Pf1N1B4_5415 0.49 -0.3 155,628 + Pf1N1B4_5415 0.71 -0.8 155,628 + Pf1N1B4_5415 0.71 -0.8 155,629 - Pf1N1B4_5415 0.71 -0.6 155,804 + -0.2 155,805 - +0.2 155,805 - -0.6 155,805 - +0.1 155,825 + +0.7 155,825 + +1.0 155,826 - +0.4 155,826 - +1.0 155,829 + +1.0 155,830 - +0.2 155,862 + Pf1N1B4_5416 0.10 -1.0 155,862 + Pf1N1B4_5416 0.10 -1.2 155,862 + Pf1N1B4_5416 0.10 +0.6 155,862 + Pf1N1B4_5416 0.10 +0.4 155,862 + Pf1N1B4_5416 0.10 -1.8 155,862 + Pf1N1B4_5416 0.10 +0.6 155,862 + Pf1N1B4_5416 0.10 -0.6 155,863 - +0.7 155,930 + Pf1N1B4_5416 0.30 +1.0 155,931 - Pf1N1B4_5416 0.30 -0.6 155,958 + Pf1N1B4_5416 0.38 -1.4 155,958 + Pf1N1B4_5416 0.38 +0.5 155,958 + Pf1N1B4_5416 0.38 -1.1 155,958 + Pf1N1B4_5416 0.38 +1.0 155,959 - Pf1N1B4_5416 0.39 +0.1 155,959 - Pf1N1B4_5416 0.39 +1.0 156,051 + Pf1N1B4_5416 0.66 +0.6 156,051 + Pf1N1B4_5416 0.66 +0.4 156,051 + Pf1N1B4_5416 0.66 +0.0 156,064 + Pf1N1B4_5416 0.70 -0.2 156,064 + Pf1N1B4_5416 0.70 +1.6 156,064 + Pf1N1B4_5416 0.70 +1.7 156,065 - Pf1N1B4_5416 0.70 +1.5 156,065 - +1.0 156,065 - Pf1N1B4_5416 0.70 +0.7 156,065 - Pf1N1B4_5416 0.70 -0.0 156,065 - Pf1N1B4_5416 0.70 +1.7 156,148 + -1.0 156,180 - +0.6 156,367 + Pf1N1B4_5417 0.12 +1.0 156,367 + +1.0 156,367 + Pf1N1B4_5417 0.12 -1.0 156,368 - Pf1N1B4_5417 0.12 +1.2 156,393 + Pf1N1B4_5417 0.14 -1.8 156,394 - Pf1N1B4_5417 0.14 +1.7 156,405 + -0.0 156,476 + Pf1N1B4_5417 0.19 +0.5 156,476 + Pf1N1B4_5417 0.19 +2.0 156,545 + Pf1N1B4_5417 0.24 -1.0 156,545 + Pf1N1B4_5417 0.24 +0.5 156,545 + Pf1N1B4_5417 0.24 +0.4 156,546 - Pf1N1B4_5417 0.24 +0.0 156,581 + Pf1N1B4_5417 0.26 +1.7 156,581 + Pf1N1B4_5417 0.26 -0.1 156,581 + Pf1N1B4_5417 0.26 -2.0 156,582 - Pf1N1B4_5417 0.26 -0.5
Or see this region's nucleotide sequence