Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4446

Experiment: D-Gluconic Acid (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4445 and Pf1N1B4_4446 are separated by 164 nucleotidesPf1N1B4_4446 and Pf1N1B4_4447 are separated by 115 nucleotidesPf1N1B4_4447 and Pf1N1B4_4448 are separated by 149 nucleotides Pf1N1B4_4445 - acetyltransferase, GNAT family, at 4,904,249 to 4,904,788 _4445 Pf1N1B4_4446 - DNA topoisomerase III (EC 5.99.1.2), at 4,904,953 to 4,906,902 _4446 Pf1N1B4_4447 - FIG00904572: hypothetical protein, at 4,907,018 to 4,907,347 _4447 Pf1N1B4_4448 - COGs COG2343, at 4,907,497 to 4,907,859 _4448 Position (kb) 4904 4905 4906 4907Strain fitness (log2 ratio) -2 -1 0 1 2 3at 4903.984 kb on - strandat 4904.045 kb on + strandat 4904.066 kb on - strandat 4904.069 kb on + strandat 4904.069 kb on + strandat 4904.070 kb on - strandat 4904.366 kb on + strand, within Pf1N1B4_4445at 4904.366 kb on + strand, within Pf1N1B4_4445at 4904.366 kb on + strand, within Pf1N1B4_4445at 4904.366 kb on + strand, within Pf1N1B4_4445at 4904.367 kb on - strand, within Pf1N1B4_4445at 4904.622 kb on + strand, within Pf1N1B4_4445at 4904.623 kb on - strand, within Pf1N1B4_4445at 4904.694 kb on + strandat 4904.695 kb on - strand, within Pf1N1B4_4445at 4904.700 kb on + strand, within Pf1N1B4_4445at 4904.700 kb on + strand, within Pf1N1B4_4445at 4904.842 kb on - strandat 4904.881 kb on - strandat 4904.925 kb on + strandat 4905.716 kb on + strand, within Pf1N1B4_4446at 4906.446 kb on - strand, within Pf1N1B4_4446at 4906.656 kb on - strand, within Pf1N1B4_4446at 4906.656 kb on - strand, within Pf1N1B4_4446at 4906.737 kb on - strandat 4906.749 kb on - strandat 4906.893 kb on - strandat 4906.893 kb on - strandat 4906.985 kb on + strandat 4907.215 kb on - strand, within Pf1N1B4_4447at 4907.446 kb on - strandat 4907.447 kb on + strandat 4907.447 kb on + strandat 4907.448 kb on - strandat 4907.448 kb on - strandat 4907.448 kb on - strandat 4907.491 kb on + strandat 4907.491 kb on + strandat 4907.491 kb on + strandat 4907.491 kb on + strandat 4907.491 kb on + strandat 4907.492 kb on - strandat 4907.492 kb on - strandat 4907.496 kb on + strandat 4907.497 kb on - strandat 4907.635 kb on + strand, within Pf1N1B4_4448at 4907.635 kb on + strand, within Pf1N1B4_4448at 4907.636 kb on - strandat 4907.636 kb on - strand, within Pf1N1B4_4448at 4907.701 kb on + strandat 4907.719 kb on + strand, within Pf1N1B4_4448at 4907.719 kb on + strand, within Pf1N1B4_4448at 4907.770 kb on + strand, within Pf1N1B4_4448at 4907.863 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Gluconic Acid (C)
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4,903,984 - +0.5
4,904,045 + -0.2
4,904,066 - +0.5
4,904,069 + -1.0
4,904,069 + -0.4
4,904,070 - -0.7
4,904,366 + Pf1N1B4_4445 0.22 +2.1
4,904,366 + Pf1N1B4_4445 0.22 -0.3
4,904,366 + Pf1N1B4_4445 0.22 +0.2
4,904,366 + Pf1N1B4_4445 0.22 -0.1
4,904,367 - Pf1N1B4_4445 0.22 +1.9
4,904,622 + Pf1N1B4_4445 0.69 +0.3
4,904,623 - Pf1N1B4_4445 0.69 +0.8
4,904,694 + +0.8
4,904,695 - Pf1N1B4_4445 0.83 +1.5
4,904,700 + Pf1N1B4_4445 0.84 +0.0
4,904,700 + Pf1N1B4_4445 0.84 -1.2
4,904,842 - +1.8
4,904,881 - -0.0
4,904,925 + +1.1
4,905,716 + Pf1N1B4_4446 0.39 -1.1
4,906,446 - Pf1N1B4_4446 0.77 -1.2
4,906,656 - Pf1N1B4_4446 0.87 -0.4
4,906,656 - Pf1N1B4_4446 0.87 -1.7
4,906,737 - +1.8
4,906,749 - -1.3
4,906,893 - -1.1
4,906,893 - -0.8
4,906,985 + -0.1
4,907,215 - Pf1N1B4_4447 0.60 +0.7
4,907,446 - -1.5
4,907,447 + +2.6
4,907,447 + -0.6
4,907,448 - +0.8
4,907,448 - +0.8
4,907,448 - -0.2
4,907,491 + -0.2
4,907,491 + +0.5
4,907,491 + -0.3
4,907,491 + -2.3
4,907,491 + +0.0
4,907,492 - -0.5
4,907,492 - -1.2
4,907,496 + -0.5
4,907,497 - -0.4
4,907,635 + Pf1N1B4_4448 0.38 +0.8
4,907,635 + Pf1N1B4_4448 0.38 -0.5
4,907,636 - +0.2
4,907,636 - Pf1N1B4_4448 0.38 +0.0
4,907,701 + +3.1
4,907,719 + Pf1N1B4_4448 0.61 -0.4
4,907,719 + Pf1N1B4_4448 0.61 +0.2
4,907,770 + Pf1N1B4_4448 0.75 -0.2
4,907,863 - +0.1

Or see this region's nucleotide sequence