Experiment: D-Gluconic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_406 and Pf1N1B4_407 are separated by 220 nucleotides Pf1N1B4_407 and Pf1N1B4_408 overlap by 4 nucleotides Pf1N1B4_408 and Pf1N1B4_409 are separated by 245 nucleotides
Pf1N1B4_406 - Purine-cytosine permease, at 474,219 to 475,685
_406
Pf1N1B4_407 - ADP-ribosylglycohydrolase YegU (EC 3.2.-.-), at 475,906 to 476,904
_407
Pf1N1B4_408 - Transcriptional regulator, GntR family, at 476,901 to 477,578
_408
Pf1N1B4_409 - ABC transporter for D-galactose/L-arabinose, permease component <small>(from data)</small>, at 477,824 to 478,792
_409
Position (kb) 475
476
477 Strain fitness (log2 ratio) -2
-1
0
1
2 at 475.135 kb on + strand, within Pf1N1B4_406 at 475.135 kb on + strand, within Pf1N1B4_406 at 475.135 kb on + strand, within Pf1N1B4_406 at 475.135 kb on + strand, within Pf1N1B4_406 at 475.135 kb on + strand, within Pf1N1B4_406 at 475.135 kb on + strand at 475.136 kb on - strand, within Pf1N1B4_406 at 475.136 kb on - strand, within Pf1N1B4_406 at 475.136 kb on - strand, within Pf1N1B4_406 at 475.136 kb on - strand, within Pf1N1B4_406 at 475.136 kb on - strand, within Pf1N1B4_406 at 475.136 kb on - strand, within Pf1N1B4_406 at 475.136 kb on - strand, within Pf1N1B4_406 at 475.563 kb on - strand at 475.685 kb on + strand at 475.685 kb on + strand at 475.685 kb on + strand at 475.685 kb on + strand at 475.686 kb on - strand at 475.686 kb on - strand at 475.686 kb on - strand at 475.686 kb on - strand at 475.740 kb on - strand at 475.794 kb on + strand at 475.795 kb on - strand at 475.795 kb on - strand at 475.798 kb on - strand at 475.837 kb on + strand at 475.861 kb on - strand at 475.864 kb on - strand at 475.864 kb on - strand at 475.902 kb on - strand at 475.966 kb on - strand at 476.184 kb on - strand, within Pf1N1B4_407 at 476.256 kb on + strand at 476.490 kb on + strand, within Pf1N1B4_407 at 476.491 kb on - strand, within Pf1N1B4_407 at 476.491 kb on - strand, within Pf1N1B4_407 at 476.870 kb on + strand at 476.870 kb on + strand at 476.870 kb on + strand at 476.870 kb on + strand at 476.871 kb on - strand at 476.871 kb on - strand at 476.871 kb on - strand at 476.871 kb on - strand at 476.871 kb on - strand at 476.992 kb on + strand, within Pf1N1B4_408 at 476.992 kb on + strand, within Pf1N1B4_408 at 476.992 kb on + strand, within Pf1N1B4_408 at 476.992 kb on + strand, within Pf1N1B4_408 at 476.992 kb on + strand, within Pf1N1B4_408 at 476.992 kb on + strand, within Pf1N1B4_408 at 476.992 kb on + strand, within Pf1N1B4_408 at 476.992 kb on + strand, within Pf1N1B4_408 at 476.992 kb on + strand, within Pf1N1B4_408 at 476.993 kb on - strand, within Pf1N1B4_408 at 476.993 kb on - strand, within Pf1N1B4_408 at 476.993 kb on - strand, within Pf1N1B4_408 at 476.993 kb on - strand, within Pf1N1B4_408 at 477.106 kb on + strand, within Pf1N1B4_408 at 477.106 kb on + strand, within Pf1N1B4_408 at 477.106 kb on + strand, within Pf1N1B4_408 at 477.106 kb on + strand, within Pf1N1B4_408 at 477.324 kb on - strand, within Pf1N1B4_408 at 477.522 kb on + strand at 477.522 kb on + strand at 477.522 kb on + strand at 477.522 kb on + strand at 477.608 kb on + strand at 477.609 kb on - strand at 477.616 kb on + strand at 477.616 kb on + strand at 477.616 kb on + strand at 477.616 kb on + strand at 477.616 kb on + strand at 477.616 kb on + strand at 477.616 kb on + strand at 477.616 kb on + strand at 477.616 kb on + strand at 477.616 kb on + strand at 477.617 kb on - strand at 477.617 kb on - strand at 477.617 kb on - strand at 477.617 kb on - strand at 477.617 kb on - strand at 477.617 kb on - strand at 477.617 kb on - strand at 477.617 kb on - strand at 477.637 kb on + strand at 477.637 kb on + strand at 477.637 kb on + strand at 477.637 kb on + strand at 477.667 kb on + strand at 477.668 kb on - strand at 477.886 kb on - strand at 477.886 kb on - strand at 477.886 kb on - strand at 477.886 kb on - strand at 477.891 kb on + strand at 477.892 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Gluconic Acid (C) remove 475,135 + Pf1N1B4_406 0.62 -0.7 475,135 + Pf1N1B4_406 0.62 -0.8 475,135 + Pf1N1B4_406 0.62 +0.4 475,135 + Pf1N1B4_406 0.62 -1.3 475,135 + Pf1N1B4_406 0.62 +0.1 475,135 + -0.7 475,136 - Pf1N1B4_406 0.63 -0.5 475,136 - Pf1N1B4_406 0.63 -0.6 475,136 - Pf1N1B4_406 0.63 -0.5 475,136 - Pf1N1B4_406 0.63 +0.4 475,136 - Pf1N1B4_406 0.63 +0.7 475,136 - Pf1N1B4_406 0.63 +0.8 475,136 - Pf1N1B4_406 0.63 +0.9 475,563 - +0.2 475,685 + +0.6 475,685 + -1.9 475,685 + -0.5 475,685 + -0.1 475,686 - +0.1 475,686 - -1.9 475,686 - -0.6 475,686 - +0.0 475,740 - -0.4 475,794 + -0.5 475,795 - +0.5 475,795 - +1.5 475,798 - -2.1 475,837 + -0.9 475,861 - +0.5 475,864 - +0.9 475,864 - +0.3 475,902 - +0.5 475,966 - +1.1 476,184 - Pf1N1B4_407 0.28 -0.6 476,256 + -1.5 476,490 + Pf1N1B4_407 0.58 -0.9 476,491 - Pf1N1B4_407 0.59 -2.2 476,491 - Pf1N1B4_407 0.59 -0.1 476,870 + -0.0 476,870 + +2.1 476,870 + -0.6 476,870 + -0.2 476,871 - -0.1 476,871 - +0.8 476,871 - +1.1 476,871 - +0.2 476,871 - -0.5 476,992 + Pf1N1B4_408 0.13 +0.5 476,992 + Pf1N1B4_408 0.13 +0.1 476,992 + Pf1N1B4_408 0.13 -0.3 476,992 + Pf1N1B4_408 0.13 +0.5 476,992 + Pf1N1B4_408 0.13 +1.1 476,992 + Pf1N1B4_408 0.13 +0.8 476,992 + Pf1N1B4_408 0.13 -0.7 476,992 + Pf1N1B4_408 0.13 -0.6 476,992 + Pf1N1B4_408 0.13 +1.1 476,993 - Pf1N1B4_408 0.14 -0.3 476,993 - Pf1N1B4_408 0.14 -0.1 476,993 - Pf1N1B4_408 0.14 -0.2 476,993 - Pf1N1B4_408 0.14 +0.2 477,106 + Pf1N1B4_408 0.30 +0.9 477,106 + Pf1N1B4_408 0.30 +0.1 477,106 + Pf1N1B4_408 0.30 -1.7 477,106 + Pf1N1B4_408 0.30 -1.9 477,324 - Pf1N1B4_408 0.62 -0.3 477,522 + -0.9 477,522 + -0.3 477,522 + +0.5 477,522 + +0.1 477,608 + +1.1 477,609 - -0.9 477,616 + +1.0 477,616 + +1.1 477,616 + +0.1 477,616 + -0.3 477,616 + -0.3 477,616 + -2.2 477,616 + +0.4 477,616 + +0.9 477,616 + -0.2 477,616 + +0.7 477,617 - -0.1 477,617 - -2.2 477,617 - +0.3 477,617 - +1.7 477,617 - -1.2 477,617 - -0.9 477,617 - -1.3 477,617 - -0.2 477,637 + -0.3 477,637 + +1.1 477,637 + +0.7 477,637 + -1.4 477,667 + -0.2 477,668 - -0.5 477,886 - +0.1 477,886 - -0.2 477,886 - +0.5 477,886 - +0.1 477,891 + -0.5 477,892 - +0.9
Or see this region's nucleotide sequence