Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4039

Experiment: D-Gluconic Acid (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4037 and Pf1N1B4_4038 overlap by 4 nucleotidesPf1N1B4_4038 and Pf1N1B4_4039 are separated by 2 nucleotidesPf1N1B4_4039 and Pf1N1B4_4040 are separated by 72 nucleotidesPf1N1B4_4040 and Pf1N1B4_4041 are separated by 10 nucleotides Pf1N1B4_4037 - NADH-ubiquinone oxidoreductase chain F (EC 1.6.5.3), at 4,488,385 to 4,489,740 _4037 Pf1N1B4_4038 - NADH-ubiquinone oxidoreductase chain E (EC 1.6.5.3), at 4,489,737 to 4,490,234 _4038 Pf1N1B4_4039 - NADH-ubiquinone oxidoreductase chain C (EC 1.6.5.3) / NADH-ubiquinone oxidoreductase chain D (EC 1.6.5.3), at 4,490,237 to 4,492,021 _4039 Pf1N1B4_4040 - NADH-ubiquinone oxidoreductase chain B (EC 1.6.5.3), at 4,492,094 to 4,492,768 _4040 Pf1N1B4_4041 - NADH ubiquinone oxidoreductase chain A (EC 1.6.5.3), at 4,492,779 to 4,493,192 _4041 Position (kb) 4490 4491 4492 4493Strain fitness (log2 ratio) -2 -1 0 1 2 3at 4489.310 kb on + strand, within Pf1N1B4_4037at 4489.741 kb on - strandat 4489.741 kb on - strandat 4489.741 kb on - strandat 4489.745 kb on - strandat 4490.011 kb on + strand, within Pf1N1B4_4038at 4490.011 kb on + strand, within Pf1N1B4_4038at 4490.012 kb on - strand, within Pf1N1B4_4038at 4490.273 kb on - strandat 4490.354 kb on - strandat 4490.404 kb on - strandat 4490.404 kb on - strandat 4490.563 kb on - strand, within Pf1N1B4_4039at 4490.950 kb on - strand, within Pf1N1B4_4039at 4490.998 kb on - strand, within Pf1N1B4_4039at 4491.060 kb on + strand, within Pf1N1B4_4039at 4491.060 kb on + strand, within Pf1N1B4_4039at 4491.060 kb on + strand, within Pf1N1B4_4039at 4491.061 kb on - strand, within Pf1N1B4_4039at 4491.061 kb on - strand, within Pf1N1B4_4039at 4491.061 kb on - strand, within Pf1N1B4_4039at 4491.061 kb on - strand, within Pf1N1B4_4039at 4491.377 kb on + strand, within Pf1N1B4_4039at 4491.377 kb on + strand, within Pf1N1B4_4039at 4491.378 kb on - strand, within Pf1N1B4_4039at 4491.378 kb on - strand, within Pf1N1B4_4039at 4491.393 kb on + strand, within Pf1N1B4_4039at 4491.393 kb on + strand, within Pf1N1B4_4039at 4491.604 kb on - strand, within Pf1N1B4_4039at 4491.628 kb on - strand, within Pf1N1B4_4039at 4491.704 kb on + strand, within Pf1N1B4_4039at 4491.704 kb on + strand, within Pf1N1B4_4039at 4491.705 kb on - strand, within Pf1N1B4_4039at 4491.713 kb on - strand, within Pf1N1B4_4039at 4491.713 kb on - strand, within Pf1N1B4_4039at 4491.716 kb on - strand, within Pf1N1B4_4039at 4491.739 kb on - strand, within Pf1N1B4_4039at 4491.816 kb on + strand, within Pf1N1B4_4039at 4491.817 kb on - strand, within Pf1N1B4_4039at 4491.817 kb on - strand, within Pf1N1B4_4039at 4491.817 kb on - strand, within Pf1N1B4_4039at 4491.828 kb on + strand, within Pf1N1B4_4039at 4491.829 kb on - strand, within Pf1N1B4_4039at 4491.987 kb on + strandat 4491.988 kb on - strandat 4492.000 kb on - strandat 4492.000 kb on - strandat 4492.025 kb on + strandat 4492.025 kb on + strandat 4492.026 kb on - strandat 4492.026 kb on - strandat 4492.026 kb on - strandat 4492.026 kb on - strandat 4492.048 kb on - strandat 4492.048 kb on - strandat 4492.048 kb on - strandat 4492.048 kb on - strandat 4492.079 kb on - strandat 4492.079 kb on - strandat 4492.079 kb on - strandat 4492.079 kb on - strandat 4492.079 kb on - strandat 4492.133 kb on - strandat 4492.296 kb on + strand, within Pf1N1B4_4040at 4492.297 kb on - strand, within Pf1N1B4_4040at 4492.350 kb on + strand, within Pf1N1B4_4040at 4492.351 kb on - strand, within Pf1N1B4_4040at 4492.396 kb on + strandat 4492.563 kb on + strand, within Pf1N1B4_4040at 4492.585 kb on + strand, within Pf1N1B4_4040at 4492.586 kb on - strand, within Pf1N1B4_4040at 4492.761 kb on + strandat 4492.762 kb on - strandat 4492.853 kb on - strand, within Pf1N1B4_4041at 4492.853 kb on - strand, within Pf1N1B4_4041at 4492.853 kb on - strand, within Pf1N1B4_4041at 4492.879 kb on - strand, within Pf1N1B4_4041at 4492.994 kb on - strand, within Pf1N1B4_4041at 4492.994 kb on - strand, within Pf1N1B4_4041

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Gluconic Acid (C)
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4,489,310 + Pf1N1B4_4037 0.68 +0.3
4,489,741 - +2.1
4,489,741 - -0.1
4,489,741 - -1.7
4,489,745 - -0.9
4,490,011 + Pf1N1B4_4038 0.55 -2.4
4,490,011 + Pf1N1B4_4038 0.55 -1.1
4,490,012 - Pf1N1B4_4038 0.55 +0.9
4,490,273 - -0.2
4,490,354 - +2.9
4,490,404 - -2.2
4,490,404 - +0.9
4,490,563 - Pf1N1B4_4039 0.18 +0.1
4,490,950 - Pf1N1B4_4039 0.40 -0.2
4,490,998 - Pf1N1B4_4039 0.43 -1.1
4,491,060 + Pf1N1B4_4039 0.46 -0.5
4,491,060 + Pf1N1B4_4039 0.46 -1.1
4,491,060 + Pf1N1B4_4039 0.46 -0.6
4,491,061 - Pf1N1B4_4039 0.46 -0.7
4,491,061 - Pf1N1B4_4039 0.46 -0.5
4,491,061 - Pf1N1B4_4039 0.46 -0.1
4,491,061 - Pf1N1B4_4039 0.46 -0.1
4,491,377 + Pf1N1B4_4039 0.64 +0.6
4,491,377 + Pf1N1B4_4039 0.64 -0.1
4,491,378 - Pf1N1B4_4039 0.64 -1.4
4,491,378 - Pf1N1B4_4039 0.64 -0.1
4,491,393 + Pf1N1B4_4039 0.65 -1.7
4,491,393 + Pf1N1B4_4039 0.65 -0.5
4,491,604 - Pf1N1B4_4039 0.77 -1.1
4,491,628 - Pf1N1B4_4039 0.78 -0.1
4,491,704 + Pf1N1B4_4039 0.82 -0.5
4,491,704 + Pf1N1B4_4039 0.82 -0.4
4,491,705 - Pf1N1B4_4039 0.82 -0.7
4,491,713 - Pf1N1B4_4039 0.83 -0.2
4,491,713 - Pf1N1B4_4039 0.83 -0.9
4,491,716 - Pf1N1B4_4039 0.83 -1.4
4,491,739 - Pf1N1B4_4039 0.84 -0.1
4,491,816 + Pf1N1B4_4039 0.88 -0.1
4,491,817 - Pf1N1B4_4039 0.89 +0.5
4,491,817 - Pf1N1B4_4039 0.89 -0.5
4,491,817 - Pf1N1B4_4039 0.89 -0.7
4,491,828 + Pf1N1B4_4039 0.89 -2.4
4,491,829 - Pf1N1B4_4039 0.89 -0.3
4,491,987 + -1.6
4,491,988 - +0.3
4,492,000 - +0.9
4,492,000 - -0.8
4,492,025 + -0.7
4,492,025 + -0.1
4,492,026 - -0.7
4,492,026 - -0.7
4,492,026 - -0.1
4,492,026 - +0.2
4,492,048 - -0.1
4,492,048 - -1.1
4,492,048 - -0.4
4,492,048 - -0.5
4,492,079 - +0.6
4,492,079 - -0.1
4,492,079 - -0.1
4,492,079 - +0.1
4,492,079 - -2.1
4,492,133 - -0.1
4,492,296 + Pf1N1B4_4040 0.30 +1.9
4,492,297 - Pf1N1B4_4040 0.30 -0.9
4,492,350 + Pf1N1B4_4040 0.38 -0.1
4,492,351 - Pf1N1B4_4040 0.38 -0.1
4,492,396 + +0.3
4,492,563 + Pf1N1B4_4040 0.69 -1.2
4,492,585 + Pf1N1B4_4040 0.73 -2.7
4,492,586 - Pf1N1B4_4040 0.73 -2.4
4,492,761 + -1.5
4,492,762 - +0.6
4,492,853 - Pf1N1B4_4041 0.18 -1.2
4,492,853 - Pf1N1B4_4041 0.18 -0.2
4,492,853 - Pf1N1B4_4041 0.18 -0.1
4,492,879 - Pf1N1B4_4041 0.24 -0.6
4,492,994 - Pf1N1B4_4041 0.52 -0.3
4,492,994 - Pf1N1B4_4041 0.52 -1.4

Or see this region's nucleotide sequence