Experiment: D-Gluconic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3865 and Pf1N1B4_3866 are separated by 137 nucleotides Pf1N1B4_3866 and Pf1N1B4_3867 are separated by 79 nucleotides Pf1N1B4_3867 and Pf1N1B4_3868 are separated by 93 nucleotides
Pf1N1B4_3865 - Butyryl-CoA dehydrogenase (EC 1.3.99.2), at 4,294,629 to 4,295,777
_3865
Pf1N1B4_3866 - Adenine phosphoribosyltransferase (EC 2.4.2.7), at 4,295,915 to 4,296,463
_3866
Pf1N1B4_3867 - Fumarate and nitrate reduction regulatory protein, at 4,296,543 to 4,297,277
_3867
Pf1N1B4_3868 - Coproporphyrinogen III oxidase, oxygen-independent (EC 1.3.99.22), at 4,297,371 to 4,298,753
_3868
Position (kb)
4295
4296
4297 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4295.535 kb on + strand, within Pf1N1B4_3865 at 4295.664 kb on + strand at 4295.665 kb on - strand at 4295.665 kb on - strand at 4295.665 kb on - strand at 4295.665 kb on - strand at 4295.665 kb on - strand at 4295.665 kb on - strand at 4295.666 kb on + strand at 4295.666 kb on + strand at 4295.666 kb on + strand at 4295.667 kb on - strand at 4295.667 kb on - strand at 4295.667 kb on - strand at 4295.667 kb on - strand at 4295.753 kb on + strand at 4295.753 kb on + strand at 4295.760 kb on - strand at 4295.843 kb on + strand at 4295.843 kb on + strand at 4295.843 kb on + strand at 4295.843 kb on + strand at 4295.843 kb on + strand at 4295.844 kb on - strand at 4295.844 kb on - strand at 4295.844 kb on - strand at 4295.854 kb on + strand at 4295.854 kb on + strand at 4295.855 kb on - strand at 4295.855 kb on - strand at 4295.855 kb on - strand at 4295.855 kb on - strand at 4295.904 kb on - strand at 4295.916 kb on + strand at 4295.916 kb on + strand at 4295.916 kb on + strand at 4295.916 kb on + strand at 4295.916 kb on + strand at 4295.917 kb on - strand at 4295.917 kb on - strand at 4295.917 kb on - strand at 4295.917 kb on - strand at 4295.917 kb on - strand at 4295.921 kb on - strand at 4295.921 kb on - strand at 4295.954 kb on - strand at 4295.954 kb on - strand at 4295.954 kb on - strand at 4295.954 kb on - strand at 4296.221 kb on + strand, within Pf1N1B4_3866 at 4296.222 kb on - strand, within Pf1N1B4_3866 at 4296.499 kb on + strand at 4296.499 kb on + strand at 4296.596 kb on + strand at 4296.597 kb on - strand at 4296.600 kb on + strand at 4296.600 kb on + strand at 4296.601 kb on - strand at 4296.685 kb on + strand, within Pf1N1B4_3867 at 4296.686 kb on - strand, within Pf1N1B4_3867 at 4297.307 kb on - strand at 4297.373 kb on - strand at 4297.373 kb on - strand at 4297.373 kb on - strand at 4297.373 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Gluconic Acid (C) remove 4,295,535 + Pf1N1B4_3865 0.79 +0.4 4,295,664 + -0.6 4,295,665 - +0.2 4,295,665 - +1.0 4,295,665 - +0.4 4,295,665 - +0.0 4,295,665 - -0.9 4,295,665 - +1.0 4,295,666 + -0.7 4,295,666 + +0.9 4,295,666 + -1.0 4,295,667 - +1.5 4,295,667 - +0.9 4,295,667 - +0.3 4,295,667 - +1.3 4,295,753 + +1.3 4,295,753 + +1.0 4,295,760 - +0.2 4,295,843 + -0.6 4,295,843 + +1.4 4,295,843 + +0.8 4,295,843 + -1.2 4,295,843 + -0.4 4,295,844 - -0.3 4,295,844 - +1.0 4,295,844 - -0.2 4,295,854 + -1.4 4,295,854 + +1.0 4,295,855 - +2.0 4,295,855 - +0.6 4,295,855 - +0.4 4,295,855 - +0.3 4,295,904 - -0.4 4,295,916 + +0.7 4,295,916 + -0.6 4,295,916 + -0.8 4,295,916 + -0.4 4,295,916 + +0.6 4,295,917 - -0.3 4,295,917 - +0.4 4,295,917 - -0.1 4,295,917 - -1.5 4,295,917 - -0.3 4,295,921 - +0.0 4,295,921 - +0.4 4,295,954 - +0.7 4,295,954 - +0.2 4,295,954 - +1.0 4,295,954 - -0.3 4,296,221 + Pf1N1B4_3866 0.56 -0.7 4,296,222 - Pf1N1B4_3866 0.56 -1.4 4,296,499 + +0.5 4,296,499 + -1.0 4,296,596 + +0.9 4,296,597 - +0.4 4,296,600 + -1.1 4,296,600 + -2.4 4,296,601 - -1.3 4,296,685 + Pf1N1B4_3867 0.19 -1.4 4,296,686 - Pf1N1B4_3867 0.19 +1.6 4,297,307 - -1.3 4,297,373 - +1.2 4,297,373 - +1.4 4,297,373 - +1.4 4,297,373 - -0.0
Or see this region's nucleotide sequence