Experiment: D-Gluconic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3831 and Pf1N1B4_3832 are separated by 290 nucleotides Pf1N1B4_3832 and Pf1N1B4_3833 are separated by 107 nucleotides Pf1N1B4_3833 and Pf1N1B4_3834 are separated by 170 nucleotides Pf1N1B4_3834 and Pf1N1B4_3835 are separated by 10 nucleotides
Pf1N1B4_3831 - CmpX, at 4,244,888 to 4,245,712
_3831
Pf1N1B4_3832 - DNA-directed RNA polymerase specialized sigma subunit, sigma24-like, at 4,246,003 to 4,246,389
_3832
Pf1N1B4_3833 - Major porin and structural outer membrane porin OprF, at 4,246,497 to 4,247,531
_3833
Pf1N1B4_3834 - Uroporphyrinogen-III methyltransferase (EC 2.1.1.107), at 4,247,702 to 4,248,469
_3834
Pf1N1B4_3835 - Nitrate reductase (EC 1.7.99.4) <small>(from data)</small>, at 4,248,480 to 4,251,194
_3835
Position (kb) 4246
4247
4248 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4245.548 kb on + strand, within Pf1N1B4_3831 at 4245.548 kb on + strand, within Pf1N1B4_3831 at 4245.548 kb on + strand, within Pf1N1B4_3831 at 4245.548 kb on + strand, within Pf1N1B4_3831 at 4245.548 kb on + strand, within Pf1N1B4_3831 at 4245.548 kb on + strand, within Pf1N1B4_3831 at 4245.548 kb on + strand, within Pf1N1B4_3831 at 4245.548 kb on + strand, within Pf1N1B4_3831 at 4245.548 kb on + strand at 4245.549 kb on - strand, within Pf1N1B4_3831 at 4245.549 kb on - strand, within Pf1N1B4_3831 at 4245.563 kb on + strand, within Pf1N1B4_3831 at 4245.563 kb on + strand at 4245.563 kb on + strand, within Pf1N1B4_3831 at 4245.563 kb on + strand, within Pf1N1B4_3831 at 4245.564 kb on - strand, within Pf1N1B4_3831 at 4245.564 kb on - strand at 4245.728 kb on + strand at 4245.733 kb on + strand at 4245.733 kb on + strand at 4245.733 kb on + strand at 4245.733 kb on + strand at 4245.733 kb on + strand at 4245.733 kb on + strand at 4245.733 kb on + strand at 4245.733 kb on + strand at 4245.733 kb on + strand at 4245.733 kb on + strand at 4245.733 kb on + strand at 4245.733 kb on + strand at 4245.733 kb on + strand at 4245.804 kb on + strand at 4246.387 kb on + strand at 4246.387 kb on + strand at 4246.387 kb on + strand at 4246.387 kb on + strand at 4246.387 kb on + strand at 4246.387 kb on + strand at 4246.387 kb on + strand at 4246.387 kb on + strand at 4246.387 kb on + strand at 4246.401 kb on + strand at 4246.401 kb on + strand at 4246.401 kb on + strand at 4246.401 kb on + strand at 4246.402 kb on - strand at 4246.408 kb on + strand at 4246.408 kb on + strand at 4246.408 kb on + strand at 4246.408 kb on + strand at 4246.408 kb on + strand at 4246.408 kb on + strand at 4246.408 kb on + strand at 4246.408 kb on + strand at 4246.408 kb on + strand at 4246.409 kb on - strand at 4246.414 kb on + strand at 4246.414 kb on + strand at 4246.674 kb on + strand, within Pf1N1B4_3833 at 4246.785 kb on + strand, within Pf1N1B4_3833 at 4247.143 kb on + strand, within Pf1N1B4_3833 at 4247.143 kb on + strand, within Pf1N1B4_3833 at 4247.143 kb on + strand, within Pf1N1B4_3833 at 4247.143 kb on + strand, within Pf1N1B4_3833 at 4247.143 kb on + strand, within Pf1N1B4_3833 at 4247.143 kb on + strand, within Pf1N1B4_3833 at 4247.143 kb on + strand, within Pf1N1B4_3833 at 4247.143 kb on + strand, within Pf1N1B4_3833 at 4247.143 kb on + strand, within Pf1N1B4_3833 at 4247.143 kb on + strand, within Pf1N1B4_3833 at 4247.143 kb on + strand, within Pf1N1B4_3833 at 4247.144 kb on - strand, within Pf1N1B4_3833 at 4247.237 kb on + strand, within Pf1N1B4_3833 at 4247.283 kb on + strand, within Pf1N1B4_3833 at 4247.297 kb on + strand, within Pf1N1B4_3833 at 4247.297 kb on + strand, within Pf1N1B4_3833 at 4247.297 kb on + strand, within Pf1N1B4_3833 at 4247.297 kb on + strand, within Pf1N1B4_3833 at 4247.297 kb on + strand, within Pf1N1B4_3833 at 4247.297 kb on + strand, within Pf1N1B4_3833 at 4247.300 kb on + strand, within Pf1N1B4_3833 at 4247.300 kb on + strand, within Pf1N1B4_3833 at 4247.300 kb on + strand, within Pf1N1B4_3833 at 4247.300 kb on + strand, within Pf1N1B4_3833 at 4247.300 kb on + strand, within Pf1N1B4_3833 at 4247.300 kb on + strand, within Pf1N1B4_3833 at 4247.300 kb on + strand, within Pf1N1B4_3833 at 4247.300 kb on + strand, within Pf1N1B4_3833 at 4247.300 kb on + strand, within Pf1N1B4_3833 at 4247.300 kb on + strand, within Pf1N1B4_3833 at 4247.300 kb on + strand, within Pf1N1B4_3833 at 4247.300 kb on + strand, within Pf1N1B4_3833 at 4247.340 kb on + strand, within Pf1N1B4_3833 at 4247.340 kb on + strand, within Pf1N1B4_3833 at 4247.340 kb on + strand, within Pf1N1B4_3833 at 4247.340 kb on + strand, within Pf1N1B4_3833 at 4247.340 kb on + strand, within Pf1N1B4_3833 at 4247.340 kb on + strand, within Pf1N1B4_3833 at 4247.340 kb on + strand, within Pf1N1B4_3833 at 4247.340 kb on + strand, within Pf1N1B4_3833 at 4247.340 kb on + strand, within Pf1N1B4_3833 at 4247.340 kb on + strand, within Pf1N1B4_3833 at 4247.341 kb on - strand, within Pf1N1B4_3833 at 4247.341 kb on - strand, within Pf1N1B4_3833 at 4247.400 kb on + strand, within Pf1N1B4_3833 at 4247.400 kb on + strand, within Pf1N1B4_3833 at 4247.400 kb on + strand, within Pf1N1B4_3833 at 4247.488 kb on + strand at 4247.488 kb on + strand at 4247.500 kb on + strand at 4247.500 kb on + strand at 4247.500 kb on + strand at 4247.500 kb on + strand at 4247.519 kb on + strand at 4247.519 kb on + strand at 4247.525 kb on + strand at 4247.525 kb on + strand at 4247.529 kb on + strand at 4247.529 kb on + strand at 4247.529 kb on + strand at 4247.529 kb on + strand at 4247.529 kb on + strand at 4247.529 kb on + strand at 4247.529 kb on + strand at 4247.529 kb on + strand at 4247.529 kb on + strand at 4247.529 kb on + strand at 4247.529 kb on + strand at 4247.529 kb on + strand at 4247.529 kb on + strand at 4247.529 kb on + strand at 4247.529 kb on + strand at 4247.530 kb on - strand at 4247.530 kb on - strand at 4247.533 kb on + strand at 4247.533 kb on + strand at 4247.533 kb on + strand at 4247.533 kb on + strand at 4247.533 kb on + strand at 4247.533 kb on + strand at 4247.533 kb on + strand at 4247.533 kb on + strand at 4247.533 kb on + strand at 4247.533 kb on + strand at 4247.533 kb on + strand at 4247.533 kb on + strand at 4247.533 kb on + strand at 4247.533 kb on + strand at 4247.561 kb on + strand at 4247.561 kb on + strand at 4247.561 kb on + strand at 4247.561 kb on + strand at 4247.619 kb on + strand at 4247.619 kb on + strand at 4247.620 kb on - strand at 4247.620 kb on - strand at 4247.653 kb on - strand at 4247.653 kb on - strand at 4247.653 kb on - strand at 4247.653 kb on - strand at 4247.669 kb on + strand at 4247.669 kb on + strand at 4247.669 kb on + strand at 4247.669 kb on + strand at 4247.669 kb on + strand at 4247.670 kb on - strand at 4247.670 kb on - strand at 4247.670 kb on - strand at 4247.670 kb on - strand at 4247.670 kb on - strand at 4247.670 kb on - strand at 4247.670 kb on - strand at 4247.670 kb on - strand at 4247.670 kb on - strand at 4247.670 kb on - strand at 4247.670 kb on - strand at 4247.670 kb on - strand at 4247.670 kb on - strand at 4247.670 kb on - strand at 4247.670 kb on - strand at 4247.670 kb on - strand at 4247.670 kb on - strand at 4247.670 kb on - strand at 4247.703 kb on + strand at 4247.703 kb on + strand at 4247.703 kb on + strand at 4247.703 kb on + strand at 4247.703 kb on + strand at 4247.703 kb on + strand at 4247.703 kb on + strand at 4247.704 kb on - strand at 4247.704 kb on - strand at 4247.704 kb on - strand at 4247.704 kb on - strand at 4247.704 kb on - strand at 4247.704 kb on - strand at 4247.906 kb on - strand, within Pf1N1B4_3834 at 4247.906 kb on - strand at 4247.968 kb on + strand, within Pf1N1B4_3834 at 4247.968 kb on + strand, within Pf1N1B4_3834 at 4247.969 kb on - strand, within Pf1N1B4_3834 at 4247.969 kb on - strand, within Pf1N1B4_3834 at 4247.970 kb on + strand, within Pf1N1B4_3834 at 4247.970 kb on + strand, within Pf1N1B4_3834 at 4247.970 kb on + strand, within Pf1N1B4_3834 at 4247.970 kb on + strand, within Pf1N1B4_3834 at 4247.970 kb on + strand, within Pf1N1B4_3834 at 4247.970 kb on + strand, within Pf1N1B4_3834 at 4247.970 kb on + strand at 4247.970 kb on + strand, within Pf1N1B4_3834 at 4247.970 kb on + strand, within Pf1N1B4_3834 at 4247.970 kb on + strand at 4247.970 kb on + strand, within Pf1N1B4_3834 at 4247.970 kb on + strand, within Pf1N1B4_3834 at 4247.970 kb on + strand, within Pf1N1B4_3834 at 4247.971 kb on - strand, within Pf1N1B4_3834 at 4247.971 kb on - strand, within Pf1N1B4_3834 at 4247.971 kb on - strand, within Pf1N1B4_3834 at 4247.971 kb on - strand, within Pf1N1B4_3834 at 4247.971 kb on - strand, within Pf1N1B4_3834 at 4247.971 kb on - strand, within Pf1N1B4_3834 at 4247.971 kb on - strand, within Pf1N1B4_3834 at 4247.971 kb on - strand, within Pf1N1B4_3834 at 4247.971 kb on - strand, within Pf1N1B4_3834 at 4247.971 kb on - strand, within Pf1N1B4_3834 at 4247.971 kb on - strand, within Pf1N1B4_3834 at 4247.971 kb on - strand, within Pf1N1B4_3834 at 4247.971 kb on - strand, within Pf1N1B4_3834 at 4247.971 kb on - strand, within Pf1N1B4_3834 at 4247.971 kb on - strand, within Pf1N1B4_3834 at 4247.971 kb on - strand, within Pf1N1B4_3834 at 4247.971 kb on - strand, within Pf1N1B4_3834 at 4247.971 kb on - strand, within Pf1N1B4_3834 at 4247.971 kb on - strand, within Pf1N1B4_3834 at 4248.244 kb on + strand, within Pf1N1B4_3834 at 4248.244 kb on + strand, within Pf1N1B4_3834 at 4248.245 kb on - strand, within Pf1N1B4_3834 at 4248.245 kb on - strand, within Pf1N1B4_3834 at 4248.245 kb on - strand, within Pf1N1B4_3834 at 4248.245 kb on - strand, within Pf1N1B4_3834 at 4248.246 kb on + strand, within Pf1N1B4_3834 at 4248.469 kb on + strand at 4248.469 kb on + strand at 4248.469 kb on + strand at 4248.469 kb on + strand at 4248.469 kb on + strand at 4248.469 kb on + strand at 4248.469 kb on + strand at 4248.469 kb on + strand at 4248.469 kb on + strand at 4248.470 kb on - strand at 4248.470 kb on - strand at 4248.470 kb on - strand at 4248.470 kb on - strand at 4248.470 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Gluconic Acid (C) remove 4,245,548 + Pf1N1B4_3831 0.80 -0.7 4,245,548 + Pf1N1B4_3831 0.80 -0.4 4,245,548 + Pf1N1B4_3831 0.80 -0.4 4,245,548 + Pf1N1B4_3831 0.80 +0.6 4,245,548 + Pf1N1B4_3831 0.80 -1.4 4,245,548 + Pf1N1B4_3831 0.80 -1.8 4,245,548 + Pf1N1B4_3831 0.80 -0.5 4,245,548 + Pf1N1B4_3831 0.80 +0.5 4,245,548 + +0.0 4,245,549 - Pf1N1B4_3831 0.80 +0.0 4,245,549 - Pf1N1B4_3831 0.80 -0.4 4,245,563 + Pf1N1B4_3831 0.82 +0.0 4,245,563 + +1.6 4,245,563 + Pf1N1B4_3831 0.82 -0.9 4,245,563 + Pf1N1B4_3831 0.82 +1.3 4,245,564 - Pf1N1B4_3831 0.82 +1.0 4,245,564 - +0.4 4,245,728 + -0.8 4,245,733 + +0.8 4,245,733 + +0.0 4,245,733 + -1.2 4,245,733 + +0.0 4,245,733 + -1.0 4,245,733 + +1.0 4,245,733 + +0.0 4,245,733 + -1.8 4,245,733 + +0.3 4,245,733 + -0.2 4,245,733 + -0.2 4,245,733 + -1.8 4,245,733 + -0.8 4,245,804 + -1.2 4,246,387 + -0.1 4,246,387 + +0.2 4,246,387 + -0.5 4,246,387 + -0.1 4,246,387 + -0.3 4,246,387 + -0.1 4,246,387 + +0.7 4,246,387 + +1.3 4,246,387 + +0.2 4,246,401 + -1.4 4,246,401 + -0.1 4,246,401 + +0.0 4,246,401 + -1.8 4,246,402 - -0.1 4,246,408 + +1.9 4,246,408 + -0.5 4,246,408 + -0.2 4,246,408 + +1.4 4,246,408 + -0.1 4,246,408 + -0.4 4,246,408 + -0.4 4,246,408 + +1.7 4,246,408 + -0.9 4,246,409 - +0.6 4,246,414 + -0.6 4,246,414 + +0.5 4,246,674 + Pf1N1B4_3833 0.17 -0.2 4,246,785 + Pf1N1B4_3833 0.28 -0.6 4,247,143 + Pf1N1B4_3833 0.62 +0.0 4,247,143 + Pf1N1B4_3833 0.62 -0.6 4,247,143 + Pf1N1B4_3833 0.62 -2.6 4,247,143 + Pf1N1B4_3833 0.62 -0.6 4,247,143 + Pf1N1B4_3833 0.62 -1.1 4,247,143 + Pf1N1B4_3833 0.62 +0.4 4,247,143 + Pf1N1B4_3833 0.62 -2.0 4,247,143 + Pf1N1B4_3833 0.62 -1.8 4,247,143 + Pf1N1B4_3833 0.62 -2.0 4,247,143 + Pf1N1B4_3833 0.62 +0.4 4,247,143 + Pf1N1B4_3833 0.62 -2.2 4,247,144 - Pf1N1B4_3833 0.63 -0.6 4,247,237 + Pf1N1B4_3833 0.71 -2.2 4,247,283 + Pf1N1B4_3833 0.76 -1.7 4,247,297 + Pf1N1B4_3833 0.77 -0.6 4,247,297 + Pf1N1B4_3833 0.77 -1.6 4,247,297 + Pf1N1B4_3833 0.77 -0.6 4,247,297 + Pf1N1B4_3833 0.77 -0.6 4,247,297 + Pf1N1B4_3833 0.77 -0.4 4,247,297 + Pf1N1B4_3833 0.77 -0.6 4,247,300 + Pf1N1B4_3833 0.78 -1.6 4,247,300 + Pf1N1B4_3833 0.78 -0.8 4,247,300 + Pf1N1B4_3833 0.78 -1.8 4,247,300 + Pf1N1B4_3833 0.78 -2.6 4,247,300 + Pf1N1B4_3833 0.78 +0.4 4,247,300 + Pf1N1B4_3833 0.78 -0.6 4,247,300 + Pf1N1B4_3833 0.78 -1.3 4,247,300 + Pf1N1B4_3833 0.78 -0.4 4,247,300 + Pf1N1B4_3833 0.78 -1.0 4,247,300 + Pf1N1B4_3833 0.78 -0.6 4,247,300 + Pf1N1B4_3833 0.78 +0.4 4,247,300 + Pf1N1B4_3833 0.78 -2.7 4,247,340 + Pf1N1B4_3833 0.81 -1.2 4,247,340 + Pf1N1B4_3833 0.81 +1.0 4,247,340 + Pf1N1B4_3833 0.81 -0.6 4,247,340 + Pf1N1B4_3833 0.81 +0.4 4,247,340 + Pf1N1B4_3833 0.81 +0.4 4,247,340 + Pf1N1B4_3833 0.81 -1.0 4,247,340 + Pf1N1B4_3833 0.81 -0.4 4,247,340 + Pf1N1B4_3833 0.81 -0.6 4,247,340 + Pf1N1B4_3833 0.81 -1.9 4,247,340 + Pf1N1B4_3833 0.81 -1.3 4,247,341 - Pf1N1B4_3833 0.82 -0.8 4,247,341 - Pf1N1B4_3833 0.82 +0.0 4,247,400 + Pf1N1B4_3833 0.87 -0.6 4,247,400 + Pf1N1B4_3833 0.87 -0.6 4,247,400 + Pf1N1B4_3833 0.87 -1.6 4,247,488 + -0.8 4,247,488 + -0.6 4,247,500 + -0.8 4,247,500 + -1.3 4,247,500 + -1.8 4,247,500 + +0.4 4,247,519 + +0.7 4,247,519 + +1.6 4,247,525 + -0.2 4,247,525 + +0.0 4,247,529 + +0.2 4,247,529 + +0.8 4,247,529 + -1.3 4,247,529 + -1.2 4,247,529 + -0.4 4,247,529 + -1.6 4,247,529 + +1.0 4,247,529 + -0.4 4,247,529 + -1.7 4,247,529 + -0.7 4,247,529 + -1.2 4,247,529 + -1.2 4,247,529 + +2.6 4,247,529 + +0.0 4,247,529 + -1.7 4,247,530 - +1.0 4,247,530 - +1.0 4,247,533 + -1.6 4,247,533 + +0.1 4,247,533 + -2.4 4,247,533 + -0.5 4,247,533 + -1.1 4,247,533 + -0.8 4,247,533 + +0.7 4,247,533 + -0.6 4,247,533 + -1.3 4,247,533 + -2.9 4,247,533 + -0.4 4,247,533 + -1.3 4,247,533 + +1.0 4,247,533 + +0.0 4,247,561 + -1.2 4,247,561 + +0.4 4,247,561 + -0.2 4,247,561 + -0.6 4,247,619 + -0.6 4,247,619 + +0.1 4,247,620 - +0.2 4,247,620 - -0.3 4,247,653 - -0.1 4,247,653 - -0.1 4,247,653 - -1.2 4,247,653 - +0.4 4,247,669 + -2.0 4,247,669 + -0.4 4,247,669 + -0.2 4,247,669 + -0.5 4,247,669 + -1.0 4,247,670 - -1.4 4,247,670 - +0.0 4,247,670 - -1.0 4,247,670 - +0.0 4,247,670 - +0.7 4,247,670 - -0.2 4,247,670 - +0.8 4,247,670 - +0.0 4,247,670 - +1.0 4,247,670 - +0.1 4,247,670 - -0.9 4,247,670 - -0.1 4,247,670 - -0.4 4,247,670 - +0.0 4,247,670 - -0.7 4,247,670 - -0.8 4,247,670 - +1.0 4,247,670 - -0.2 4,247,703 + -0.3 4,247,703 + -1.6 4,247,703 + -0.8 4,247,703 + -0.3 4,247,703 + -0.2 4,247,703 + +0.6 4,247,703 + -0.3 4,247,704 - -0.4 4,247,704 - +1.2 4,247,704 - +0.0 4,247,704 - -0.2 4,247,704 - -1.0 4,247,704 - +0.4 4,247,906 - Pf1N1B4_3834 0.27 -0.1 4,247,906 - +1.0 4,247,968 + Pf1N1B4_3834 0.35 +0.6 4,247,968 + Pf1N1B4_3834 0.35 -2.2 4,247,969 - Pf1N1B4_3834 0.35 +0.3 4,247,969 - Pf1N1B4_3834 0.35 +1.0 4,247,970 + Pf1N1B4_3834 0.35 -0.2 4,247,970 + Pf1N1B4_3834 0.35 -1.0 4,247,970 + Pf1N1B4_3834 0.35 +1.0 4,247,970 + Pf1N1B4_3834 0.35 -1.4 4,247,970 + Pf1N1B4_3834 0.35 -0.3 4,247,970 + Pf1N1B4_3834 0.35 -0.8 4,247,970 + -1.0 4,247,970 + Pf1N1B4_3834 0.35 -0.1 4,247,970 + Pf1N1B4_3834 0.35 -0.3 4,247,970 + -1.0 4,247,970 + Pf1N1B4_3834 0.35 -0.4 4,247,970 + Pf1N1B4_3834 0.35 +0.4 4,247,970 + Pf1N1B4_3834 0.35 +0.8 4,247,971 - Pf1N1B4_3834 0.35 +0.6 4,247,971 - Pf1N1B4_3834 0.35 +0.2 4,247,971 - Pf1N1B4_3834 0.35 +0.2 4,247,971 - Pf1N1B4_3834 0.35 -0.6 4,247,971 - Pf1N1B4_3834 0.35 -0.7 4,247,971 - Pf1N1B4_3834 0.35 -0.7 4,247,971 - Pf1N1B4_3834 0.35 +0.7 4,247,971 - Pf1N1B4_3834 0.35 -0.4 4,247,971 - Pf1N1B4_3834 0.35 +0.1 4,247,971 - Pf1N1B4_3834 0.35 +0.0 4,247,971 - Pf1N1B4_3834 0.35 -0.2 4,247,971 - Pf1N1B4_3834 0.35 +0.2 4,247,971 - Pf1N1B4_3834 0.35 +0.2 4,247,971 - Pf1N1B4_3834 0.35 +0.4 4,247,971 - Pf1N1B4_3834 0.35 -0.5 4,247,971 - Pf1N1B4_3834 0.35 +0.1 4,247,971 - Pf1N1B4_3834 0.35 +0.8 4,247,971 - Pf1N1B4_3834 0.35 -0.9 4,247,971 - Pf1N1B4_3834 0.35 -0.9 4,248,244 + Pf1N1B4_3834 0.71 -0.3 4,248,244 + Pf1N1B4_3834 0.71 -2.7 4,248,245 - Pf1N1B4_3834 0.71 -0.1 4,248,245 - Pf1N1B4_3834 0.71 +0.0 4,248,245 - Pf1N1B4_3834 0.71 +2.0 4,248,245 - Pf1N1B4_3834 0.71 +0.7 4,248,246 + Pf1N1B4_3834 0.71 +0.9 4,248,469 + -1.3 4,248,469 + +1.3 4,248,469 + -0.4 4,248,469 + -0.2 4,248,469 + +0.3 4,248,469 + +0.4 4,248,469 + -0.5 4,248,469 + -0.4 4,248,469 + +0.5 4,248,470 - +0.4 4,248,470 - -0.1 4,248,470 - -0.5 4,248,470 - -1.6 4,248,470 - +0.3
Or see this region's nucleotide sequence