Experiment: D-Gluconic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3707 and Pf1N1B4_3708 overlap by 4 nucleotides Pf1N1B4_3708 and Pf1N1B4_3709 overlap by 4 nucleotides Pf1N1B4_3709 and Pf1N1B4_3710 overlap by 8 nucleotides Pf1N1B4_3710 and Pf1N1B4_3711 overlap by 4 nucleotides
Pf1N1B4_3707 - Cobyrinic acid A,C-diamide synthase, at 4,115,664 to 4,117,046
_3707
Pf1N1B4_3708 - Cobalamin biosynthesis protein BluB @ 5,6-dimethylbenzimidazole synthase, flavin destructase family, at 4,117,043 to 4,117,693
_3708
Pf1N1B4_3709 - Adenosylcobinamide-phosphate synthase (EC 6.3.1.10), at 4,117,690 to 4,118,598
_3709
Pf1N1B4_3710 - L-threonine 3-O-phosphate decarboxylase (EC 4.1.1.81), at 4,118,591 to 4,119,583
_3710
Pf1N1B4_3711 - Cobyric acid synthase (EC 6.3.5.10), at 4,119,580 to 4,121,073
_3711
Position (kb) 4117
4118
4119 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 4116.789 kb on + strand, within Pf1N1B4_3707 at 4116.789 kb on + strand, within Pf1N1B4_3707 at 4116.789 kb on + strand at 4116.789 kb on + strand, within Pf1N1B4_3707 at 4116.789 kb on + strand, within Pf1N1B4_3707 at 4116.789 kb on + strand, within Pf1N1B4_3707 at 4116.789 kb on + strand, within Pf1N1B4_3707 at 4116.900 kb on + strand, within Pf1N1B4_3707 at 4116.900 kb on + strand, within Pf1N1B4_3707 at 4116.900 kb on + strand at 4116.901 kb on - strand, within Pf1N1B4_3707 at 4116.901 kb on - strand, within Pf1N1B4_3707 at 4116.912 kb on + strand at 4116.912 kb on + strand at 4117.149 kb on + strand, within Pf1N1B4_3708 at 4117.149 kb on + strand, within Pf1N1B4_3708 at 4117.359 kb on + strand, within Pf1N1B4_3708 at 4117.360 kb on - strand, within Pf1N1B4_3708 at 4117.620 kb on + strand, within Pf1N1B4_3708 at 4117.664 kb on + strand at 4117.664 kb on + strand at 4117.665 kb on - strand at 4117.673 kb on + strand at 4117.674 kb on - strand at 4117.909 kb on + strand, within Pf1N1B4_3709 at 4117.909 kb on + strand, within Pf1N1B4_3709 at 4117.942 kb on + strand at 4117.942 kb on + strand at 4117.942 kb on + strand, within Pf1N1B4_3709 at 4118.284 kb on + strand at 4118.284 kb on + strand, within Pf1N1B4_3709 at 4118.284 kb on + strand, within Pf1N1B4_3709 at 4118.284 kb on + strand, within Pf1N1B4_3709 at 4118.285 kb on - strand, within Pf1N1B4_3709 at 4118.285 kb on - strand, within Pf1N1B4_3709 at 4118.315 kb on - strand, within Pf1N1B4_3709 at 4118.452 kb on + strand, within Pf1N1B4_3709 at 4118.452 kb on + strand, within Pf1N1B4_3709 at 4118.452 kb on + strand, within Pf1N1B4_3709 at 4118.591 kb on - strand at 4118.774 kb on + strand, within Pf1N1B4_3710 at 4118.774 kb on + strand, within Pf1N1B4_3710 at 4118.877 kb on - strand, within Pf1N1B4_3710 at 4118.912 kb on + strand, within Pf1N1B4_3710 at 4118.912 kb on + strand, within Pf1N1B4_3710 at 4118.913 kb on - strand, within Pf1N1B4_3710 at 4118.913 kb on - strand, within Pf1N1B4_3710 at 4118.913 kb on - strand, within Pf1N1B4_3710 at 4119.331 kb on + strand, within Pf1N1B4_3710 at 4119.375 kb on - strand, within Pf1N1B4_3710 at 4119.563 kb on + strand at 4119.564 kb on - strand at 4119.564 kb on - strand at 4119.585 kb on + strand at 4119.585 kb on + strand at 4119.586 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Gluconic Acid (C) remove 4,116,789 + Pf1N1B4_3707 0.81 -0.7 4,116,789 + Pf1N1B4_3707 0.81 -0.3 4,116,789 + -0.5 4,116,789 + Pf1N1B4_3707 0.81 +0.7 4,116,789 + Pf1N1B4_3707 0.81 +0.2 4,116,789 + Pf1N1B4_3707 0.81 -0.1 4,116,789 + Pf1N1B4_3707 0.81 +1.0 4,116,900 + Pf1N1B4_3707 0.89 -2.0 4,116,900 + Pf1N1B4_3707 0.89 +0.5 4,116,900 + +1.0 4,116,901 - Pf1N1B4_3707 0.89 -2.5 4,116,901 - Pf1N1B4_3707 0.89 +0.5 4,116,912 + -2.4 4,116,912 + -1.1 4,117,149 + Pf1N1B4_3708 0.16 -0.3 4,117,149 + Pf1N1B4_3708 0.16 +3.0 4,117,359 + Pf1N1B4_3708 0.49 -0.3 4,117,360 - Pf1N1B4_3708 0.49 -0.2 4,117,620 + Pf1N1B4_3708 0.89 -0.1 4,117,664 + +1.4 4,117,664 + +0.0 4,117,665 - +0.0 4,117,673 + +1.0 4,117,674 - -1.1 4,117,909 + Pf1N1B4_3709 0.24 +1.0 4,117,909 + Pf1N1B4_3709 0.24 -0.4 4,117,942 + +1.0 4,117,942 + -1.0 4,117,942 + Pf1N1B4_3709 0.28 +1.0 4,118,284 + -0.5 4,118,284 + Pf1N1B4_3709 0.65 -0.3 4,118,284 + Pf1N1B4_3709 0.65 -1.4 4,118,284 + Pf1N1B4_3709 0.65 -0.2 4,118,285 - Pf1N1B4_3709 0.65 -0.7 4,118,285 - Pf1N1B4_3709 0.65 +1.3 4,118,315 - Pf1N1B4_3709 0.69 -1.5 4,118,452 + Pf1N1B4_3709 0.84 -1.8 4,118,452 + Pf1N1B4_3709 0.84 -2.3 4,118,452 + Pf1N1B4_3709 0.84 -1.3 4,118,591 - +1.8 4,118,774 + Pf1N1B4_3710 0.18 -0.8 4,118,774 + Pf1N1B4_3710 0.18 -0.3 4,118,877 - Pf1N1B4_3710 0.29 -1.2 4,118,912 + Pf1N1B4_3710 0.32 -1.3 4,118,912 + Pf1N1B4_3710 0.32 -0.1 4,118,913 - Pf1N1B4_3710 0.32 -1.9 4,118,913 - Pf1N1B4_3710 0.32 -0.3 4,118,913 - Pf1N1B4_3710 0.32 -0.2 4,119,331 + Pf1N1B4_3710 0.75 +2.0 4,119,375 - Pf1N1B4_3710 0.79 -1.1 4,119,563 + -0.9 4,119,564 - +0.5 4,119,564 - +0.4 4,119,585 + -1.0 4,119,585 + +0.8 4,119,586 - -2.3
Or see this region's nucleotide sequence