Experiment: D-Gluconic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3450 and Pf1N1B4_3451 are separated by 90 nucleotides Pf1N1B4_3451 and Pf1N1B4_3452 are separated by 186 nucleotides Pf1N1B4_3452 and Pf1N1B4_5221 are separated by 62 nucleotides Pf1N1B4_5221 and Pf1N1B4_5222 are separated by 110 nucleotides Pf1N1B4_5222 and Pf1N1B4_5223 are separated by 74 nucleotides Pf1N1B4_5223 and Pf1N1B4_3453 are separated by 22 nucleotides
Pf1N1B4_3450 - Alanyl-tRNA synthetase (EC 6.1.1.7), at 3,845,825 to 3,848,446
_3450
Pf1N1B4_3451 - Aspartokinase (EC 2.7.2.4), at 3,848,537 to 3,849,778
_3451
Pf1N1B4_3452 - Carbon storage regulator, at 3,849,965 to 3,850,153
_3452
Pf1N1B4_5221 - tRNA-Ser-GCT, at 3,850,216 to 3,850,303
_5221
Pf1N1B4_5222 - tRNA-Arg-ACG, at 3,850,414 to 3,850,487
_5222
Pf1N1B4_5223 - tRNA-Arg-ACG, at 3,850,562 to 3,850,635
_5223
Pf1N1B4_3453 - hypothetical protein, at 3,850,658 to 3,850,834
_3453
Position (kb)
3848
3849
3850 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3848.430 kb on + strand at 3848.430 kb on + strand at 3848.430 kb on + strand at 3848.430 kb on + strand at 3848.442 kb on + strand at 3848.442 kb on + strand at 3848.442 kb on + strand at 3848.442 kb on + strand at 3848.442 kb on + strand at 3848.442 kb on + strand at 3848.442 kb on + strand at 3848.442 kb on + strand at 3848.442 kb on + strand at 3848.444 kb on + strand at 3848.444 kb on + strand at 3848.444 kb on + strand at 3848.444 kb on + strand at 3848.444 kb on + strand at 3848.444 kb on + strand at 3848.501 kb on + strand at 3848.501 kb on + strand at 3849.262 kb on + strand, within Pf1N1B4_3451 at 3849.262 kb on + strand, within Pf1N1B4_3451 at 3849.262 kb on + strand, within Pf1N1B4_3451 at 3849.262 kb on + strand, within Pf1N1B4_3451 at 3849.262 kb on + strand, within Pf1N1B4_3451 at 3849.265 kb on + strand, within Pf1N1B4_3451 at 3849.265 kb on + strand, within Pf1N1B4_3451 at 3849.265 kb on + strand, within Pf1N1B4_3451 at 3849.265 kb on + strand, within Pf1N1B4_3451 at 3849.265 kb on + strand, within Pf1N1B4_3451 at 3849.265 kb on + strand, within Pf1N1B4_3451 at 3849.776 kb on + strand at 3849.776 kb on + strand at 3849.776 kb on + strand at 3849.879 kb on + strand at 3849.879 kb on + strand at 3849.879 kb on + strand at 3849.879 kb on + strand at 3849.879 kb on + strand at 3849.879 kb on + strand at 3849.880 kb on - strand at 3849.964 kb on + strand at 3850.109 kb on + strand, within Pf1N1B4_3452 at 3850.109 kb on + strand, within Pf1N1B4_3452 at 3850.308 kb on + strand at 3850.308 kb on + strand at 3850.316 kb on + strand at 3850.493 kb on + strand at 3850.493 kb on + strand at 3850.493 kb on + strand at 3850.547 kb on + strand at 3850.549 kb on + strand at 3850.549 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Gluconic Acid (C) remove 3,848,430 + -0.9 3,848,430 + +0.6 3,848,430 + -0.3 3,848,430 + +1.6 3,848,442 + +0.1 3,848,442 + +0.7 3,848,442 + +0.0 3,848,442 + +0.1 3,848,442 + -0.0 3,848,442 + -0.0 3,848,442 + -0.7 3,848,442 + +0.2 3,848,442 + -0.2 3,848,444 + -1.4 3,848,444 + -0.4 3,848,444 + -0.3 3,848,444 + +1.1 3,848,444 + -0.9 3,848,444 + +1.1 3,848,501 + -0.7 3,848,501 + +0.4 3,849,262 + Pf1N1B4_3451 0.58 -0.7 3,849,262 + Pf1N1B4_3451 0.58 -1.2 3,849,262 + Pf1N1B4_3451 0.58 +2.5 3,849,262 + Pf1N1B4_3451 0.58 -1.4 3,849,262 + Pf1N1B4_3451 0.58 -0.9 3,849,265 + Pf1N1B4_3451 0.59 +0.3 3,849,265 + Pf1N1B4_3451 0.59 -2.4 3,849,265 + Pf1N1B4_3451 0.59 -1.1 3,849,265 + Pf1N1B4_3451 0.59 +1.1 3,849,265 + Pf1N1B4_3451 0.59 -0.4 3,849,265 + Pf1N1B4_3451 0.59 -0.4 3,849,776 + -1.3 3,849,776 + -1.3 3,849,776 + +0.1 3,849,879 + +0.1 3,849,879 + -0.3 3,849,879 + -0.2 3,849,879 + -0.8 3,849,879 + -0.2 3,849,879 + +0.2 3,849,880 - -1.9 3,849,964 + -1.9 3,850,109 + Pf1N1B4_3452 0.76 +0.8 3,850,109 + Pf1N1B4_3452 0.76 +1.6 3,850,308 + -2.2 3,850,308 + -1.7 3,850,316 + -0.9 3,850,493 + +0.6 3,850,493 + -0.3 3,850,493 + -0.2 3,850,547 + -0.1 3,850,549 + -1.9 3,850,549 + -1.4
Or see this region's nucleotide sequence