Experiment: D-Gluconic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_26 and Pf1N1B4_27 are separated by 337 nucleotides Pf1N1B4_27 and Pf1N1B4_28 are separated by 235 nucleotides Pf1N1B4_28 and Pf1N1B4_29 overlap by 4 nucleotides Pf1N1B4_29 and Pf1N1B4_5164 are separated by 225 nucleotides
Pf1N1B4_26 - Excinuclease ABC subunit B, at 28,200 to 30,215
_26
Pf1N1B4_27 - Biosynthetic Aromatic amino acid aminotransferase alpha (EC 2.6.1.57) @ Aromatic-amino-acid aminotransferase (EC 2.6.1.57), at 30,553 to 31,749
_27
Pf1N1B4_28 - FIG00958186: hypothetical protein, at 31,985 to 32,185
_28
Pf1N1B4_29 - FIG00967200: hypothetical protein, at 32,182 to 32,412
_29
Pf1N1B4_5164 - tRNA-Asn-GTT, at 32,638 to 32,710
_5164
Position (kb)
30
31
32 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 29.616 kb on - strand at 29.677 kb on + strand, within Pf1N1B4_26 at 29.677 kb on + strand, within Pf1N1B4_26 at 29.677 kb on + strand, within Pf1N1B4_26 at 29.678 kb on - strand, within Pf1N1B4_26 at 29.758 kb on + strand, within Pf1N1B4_26 at 29.759 kb on - strand, within Pf1N1B4_26 at 29.782 kb on + strand, within Pf1N1B4_26 at 29.918 kb on - strand, within Pf1N1B4_26 at 29.936 kb on - strand, within Pf1N1B4_26 at 29.944 kb on + strand, within Pf1N1B4_26 at 29.945 kb on - strand, within Pf1N1B4_26 at 29.957 kb on - strand, within Pf1N1B4_26 at 29.998 kb on + strand, within Pf1N1B4_26 at 29.999 kb on - strand, within Pf1N1B4_26 at 29.999 kb on - strand, within Pf1N1B4_26 at 30.198 kb on + strand at 30.215 kb on + strand at 30.215 kb on + strand at 30.216 kb on - strand at 30.216 kb on - strand at 30.216 kb on - strand at 30.216 kb on - strand at 30.216 kb on - strand at 30.216 kb on - strand at 30.216 kb on - strand at 30.216 kb on - strand at 30.216 kb on - strand at 30.219 kb on + strand at 30.219 kb on + strand at 30.219 kb on + strand at 30.220 kb on - strand at 30.220 kb on - strand at 30.220 kb on - strand at 30.220 kb on - strand at 30.220 kb on - strand at 30.220 kb on - strand at 30.220 kb on - strand at 30.220 kb on - strand at 30.220 kb on - strand at 30.355 kb on - strand at 30.464 kb on + strand at 30.465 kb on - strand at 30.466 kb on + strand at 30.467 kb on - strand at 30.467 kb on - strand at 30.467 kb on - strand at 30.467 kb on - strand at 30.469 kb on + strand at 30.469 kb on + strand at 30.469 kb on + strand at 30.469 kb on + strand at 30.469 kb on + strand at 30.469 kb on + strand at 30.469 kb on + strand at 30.470 kb on - strand at 30.494 kb on - strand at 30.551 kb on + strand at 30.664 kb on + strand at 30.778 kb on + strand, within Pf1N1B4_27 at 31.006 kb on + strand, within Pf1N1B4_27 at 31.007 kb on - strand at 31.405 kb on + strand, within Pf1N1B4_27 at 31.405 kb on + strand, within Pf1N1B4_27 at 31.405 kb on + strand, within Pf1N1B4_27 at 31.405 kb on + strand, within Pf1N1B4_27 at 31.406 kb on - strand, within Pf1N1B4_27 at 31.606 kb on + strand, within Pf1N1B4_27 at 31.631 kb on + strand at 31.631 kb on + strand at 31.660 kb on + strand at 31.660 kb on + strand at 31.660 kb on + strand at 31.660 kb on + strand at 31.660 kb on + strand at 31.661 kb on - strand at 31.661 kb on - strand at 31.661 kb on - strand at 31.661 kb on - strand at 31.762 kb on + strand at 31.781 kb on - strand at 31.797 kb on + strand at 31.798 kb on - strand at 31.810 kb on + strand at 31.892 kb on - strand at 31.952 kb on + strand at 31.952 kb on + strand at 31.958 kb on + strand at 31.958 kb on + strand at 31.959 kb on - strand at 31.959 kb on - strand at 31.959 kb on - strand at 31.959 kb on - strand at 31.959 kb on - strand at 32.001 kb on + strand at 32.002 kb on - strand at 32.144 kb on + strand, within Pf1N1B4_28 at 32.170 kb on + strand at 32.171 kb on - strand at 32.171 kb on - strand at 32.223 kb on - strand, within Pf1N1B4_29 at 32.223 kb on - strand, within Pf1N1B4_29 at 32.232 kb on - strand, within Pf1N1B4_29 at 32.232 kb on - strand, within Pf1N1B4_29 at 32.232 kb on - strand, within Pf1N1B4_29 at 32.463 kb on + strand at 32.517 kb on + strand at 32.526 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Gluconic Acid (C) remove 29,616 - +1.0 29,677 + Pf1N1B4_26 0.73 +3.3 29,677 + Pf1N1B4_26 0.73 -0.1 29,677 + Pf1N1B4_26 0.73 -0.7 29,678 - Pf1N1B4_26 0.73 -1.0 29,758 + Pf1N1B4_26 0.77 +0.4 29,759 - Pf1N1B4_26 0.77 +0.0 29,782 + Pf1N1B4_26 0.78 -0.3 29,918 - Pf1N1B4_26 0.85 -1.8 29,936 - Pf1N1B4_26 0.86 -0.3 29,944 + Pf1N1B4_26 0.87 +1.2 29,945 - Pf1N1B4_26 0.87 -1.4 29,957 - Pf1N1B4_26 0.87 +0.6 29,998 + Pf1N1B4_26 0.89 -1.6 29,999 - Pf1N1B4_26 0.89 +1.0 29,999 - Pf1N1B4_26 0.89 +0.2 30,198 + +0.0 30,215 + +1.5 30,215 + +0.6 30,216 - -1.0 30,216 - -0.4 30,216 - +2.0 30,216 - +1.0 30,216 - -1.0 30,216 - +1.3 30,216 - +0.1 30,216 - -1.3 30,216 - +0.0 30,219 + -0.1 30,219 + +0.4 30,219 + +0.1 30,220 - -0.3 30,220 - +0.0 30,220 - -1.0 30,220 - +1.2 30,220 - -1.0 30,220 - +0.6 30,220 - -0.2 30,220 - -0.5 30,220 - -0.8 30,355 - -2.4 30,464 + +0.3 30,465 - -1.3 30,466 + -0.3 30,467 - -1.8 30,467 - -2.0 30,467 - +0.0 30,467 - -1.1 30,469 + +0.8 30,469 + -0.6 30,469 + -1.8 30,469 + +1.6 30,469 + -0.8 30,469 + +0.4 30,469 + -0.4 30,470 - +0.0 30,494 - -1.8 30,551 + -1.3 30,664 + -0.6 30,778 + Pf1N1B4_27 0.19 -2.3 31,006 + Pf1N1B4_27 0.38 -1.2 31,007 - +1.0 31,405 + Pf1N1B4_27 0.71 -1.7 31,405 + Pf1N1B4_27 0.71 -0.5 31,405 + Pf1N1B4_27 0.71 -0.6 31,405 + Pf1N1B4_27 0.71 -2.4 31,406 - Pf1N1B4_27 0.71 -1.0 31,606 + Pf1N1B4_27 0.88 -0.4 31,631 + -2.6 31,631 + +0.0 31,660 + +0.0 31,660 + +0.3 31,660 + -1.6 31,660 + -0.3 31,660 + -2.3 31,661 - +0.0 31,661 - -2.2 31,661 - -0.9 31,661 - -1.3 31,762 + +1.8 31,781 - -1.0 31,797 + -0.8 31,798 - +0.3 31,810 + -1.0 31,892 - -0.3 31,952 + -0.4 31,952 + +0.4 31,958 + +0.3 31,958 + -0.6 31,959 - -1.3 31,959 - +0.6 31,959 - -0.2 31,959 - +1.7 31,959 - +0.7 32,001 + -0.4 32,002 - +0.0 32,144 + Pf1N1B4_28 0.79 +0.6 32,170 + +1.0 32,171 - -0.9 32,171 - +0.5 32,223 - Pf1N1B4_29 0.18 -0.8 32,223 - Pf1N1B4_29 0.18 +0.6 32,232 - Pf1N1B4_29 0.22 -0.3 32,232 - Pf1N1B4_29 0.22 +0.0 32,232 - Pf1N1B4_29 0.22 +0.7 32,463 + -1.1 32,517 + +0.7 32,526 - +0.0
Or see this region's nucleotide sequence