Experiment: D-Gluconic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2548 and Pf1N1B4_2549 overlap by 4 nucleotides Pf1N1B4_2549 and Pf1N1B4_2550 are separated by 148 nucleotides Pf1N1B4_2550 and Pf1N1B4_2551 are separated by 24 nucleotides
Pf1N1B4_2548 - Anthranilate phosphoribosyltransferase (EC 2.4.2.18), at 2,891,923 to 2,892,972
_2548
Pf1N1B4_2549 - Indole-3-glycerol phosphate synthase (EC 4.1.1.48), at 2,892,969 to 2,893,805
_2549
Pf1N1B4_2550 - Lipoate-protein ligase A, at 2,893,954 to 2,894,655
_2550
Pf1N1B4_2551 - Vfr transcriptional regulator / Cyclic AMP receptor protein, at 2,894,680 to 2,895,324
_2551
Position (kb)
2892
2893
2894 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2892.075 kb on + strand, within Pf1N1B4_2548 at 2892.312 kb on + strand, within Pf1N1B4_2548 at 2892.312 kb on + strand, within Pf1N1B4_2548 at 2892.312 kb on + strand, within Pf1N1B4_2548 at 2892.313 kb on - strand, within Pf1N1B4_2548 at 2892.313 kb on - strand, within Pf1N1B4_2548 at 2892.313 kb on - strand, within Pf1N1B4_2548 at 2892.313 kb on - strand, within Pf1N1B4_2548 at 2892.313 kb on - strand, within Pf1N1B4_2548 at 2892.316 kb on + strand, within Pf1N1B4_2548 at 2892.316 kb on + strand, within Pf1N1B4_2548 at 2892.316 kb on + strand, within Pf1N1B4_2548 at 2892.316 kb on + strand, within Pf1N1B4_2548 at 2892.316 kb on + strand, within Pf1N1B4_2548 at 2892.317 kb on - strand, within Pf1N1B4_2548 at 2892.317 kb on - strand, within Pf1N1B4_2548 at 2892.317 kb on - strand, within Pf1N1B4_2548 at 2892.317 kb on - strand, within Pf1N1B4_2548 at 2892.396 kb on + strand, within Pf1N1B4_2548 at 2892.397 kb on - strand, within Pf1N1B4_2548 at 2892.816 kb on + strand, within Pf1N1B4_2548 at 2892.816 kb on + strand, within Pf1N1B4_2548 at 2892.817 kb on - strand, within Pf1N1B4_2548 at 2892.835 kb on + strand, within Pf1N1B4_2548 at 2892.835 kb on + strand, within Pf1N1B4_2548 at 2892.836 kb on - strand, within Pf1N1B4_2548 at 2892.939 kb on + strand at 2892.948 kb on - strand at 2892.988 kb on + strand at 2892.995 kb on + strand at 2892.996 kb on - strand at 2893.269 kb on + strand, within Pf1N1B4_2549 at 2893.269 kb on + strand, within Pf1N1B4_2549 at 2893.269 kb on + strand, within Pf1N1B4_2549 at 2893.269 kb on + strand, within Pf1N1B4_2549 at 2893.270 kb on - strand, within Pf1N1B4_2549 at 2893.270 kb on - strand, within Pf1N1B4_2549 at 2893.270 kb on - strand, within Pf1N1B4_2549 at 2893.290 kb on + strand, within Pf1N1B4_2549 at 2893.291 kb on - strand, within Pf1N1B4_2549 at 2893.291 kb on - strand, within Pf1N1B4_2549 at 2893.338 kb on + strand, within Pf1N1B4_2549 at 2893.450 kb on + strand, within Pf1N1B4_2549 at 2893.451 kb on - strand, within Pf1N1B4_2549 at 2893.451 kb on - strand, within Pf1N1B4_2549 at 2893.451 kb on - strand, within Pf1N1B4_2549 at 2893.630 kb on + strand, within Pf1N1B4_2549 at 2893.630 kb on + strand, within Pf1N1B4_2549 at 2893.630 kb on + strand, within Pf1N1B4_2549 at 2893.630 kb on + strand, within Pf1N1B4_2549 at 2893.630 kb on + strand, within Pf1N1B4_2549 at 2893.630 kb on + strand, within Pf1N1B4_2549 at 2893.630 kb on + strand, within Pf1N1B4_2549 at 2893.630 kb on + strand, within Pf1N1B4_2549 at 2893.631 kb on - strand, within Pf1N1B4_2549 at 2893.634 kb on + strand, within Pf1N1B4_2549 at 2893.635 kb on - strand, within Pf1N1B4_2549 at 2893.692 kb on + strand, within Pf1N1B4_2549 at 2893.692 kb on + strand, within Pf1N1B4_2549 at 2893.692 kb on + strand, within Pf1N1B4_2549 at 2893.692 kb on + strand, within Pf1N1B4_2549 at 2893.692 kb on + strand, within Pf1N1B4_2549 at 2893.692 kb on + strand, within Pf1N1B4_2549 at 2893.692 kb on + strand, within Pf1N1B4_2549 at 2893.692 kb on + strand, within Pf1N1B4_2549 at 2893.693 kb on - strand, within Pf1N1B4_2549 at 2893.693 kb on - strand, within Pf1N1B4_2549 at 2893.693 kb on - strand, within Pf1N1B4_2549 at 2893.693 kb on - strand, within Pf1N1B4_2549 at 2893.793 kb on + strand at 2893.793 kb on + strand at 2893.793 kb on + strand at 2893.793 kb on + strand at 2893.794 kb on - strand at 2893.851 kb on + strand at 2893.851 kb on + strand at 2893.852 kb on - strand at 2893.915 kb on + strand at 2893.915 kb on + strand at 2893.916 kb on - strand at 2893.941 kb on + strand at 2893.942 kb on - strand at 2893.953 kb on + strand at 2893.953 kb on + strand at 2893.953 kb on + strand at 2893.954 kb on - strand at 2893.954 kb on - strand at 2893.954 kb on - strand at 2894.157 kb on - strand, within Pf1N1B4_2550 at 2894.224 kb on + strand, within Pf1N1B4_2550 at 2894.224 kb on + strand, within Pf1N1B4_2550 at 2894.225 kb on - strand, within Pf1N1B4_2550 at 2894.225 kb on - strand, within Pf1N1B4_2550 at 2894.303 kb on + strand, within Pf1N1B4_2550 at 2894.518 kb on - strand, within Pf1N1B4_2550 at 2894.653 kb on + strand at 2894.653 kb on + strand at 2894.653 kb on + strand at 2894.653 kb on + strand at 2894.654 kb on - strand at 2894.654 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Gluconic Acid (C) remove 2,892,075 + Pf1N1B4_2548 0.14 -2.2 2,892,312 + Pf1N1B4_2548 0.37 -1.0 2,892,312 + Pf1N1B4_2548 0.37 -1.5 2,892,312 + Pf1N1B4_2548 0.37 -0.8 2,892,313 - Pf1N1B4_2548 0.37 +1.5 2,892,313 - Pf1N1B4_2548 0.37 -2.0 2,892,313 - Pf1N1B4_2548 0.37 -1.4 2,892,313 - Pf1N1B4_2548 0.37 +0.2 2,892,313 - Pf1N1B4_2548 0.37 -2.9 2,892,316 + Pf1N1B4_2548 0.37 -3.2 2,892,316 + Pf1N1B4_2548 0.37 -1.0 2,892,316 + Pf1N1B4_2548 0.37 -2.6 2,892,316 + Pf1N1B4_2548 0.37 -0.8 2,892,316 + Pf1N1B4_2548 0.37 -0.0 2,892,317 - Pf1N1B4_2548 0.38 -1.9 2,892,317 - Pf1N1B4_2548 0.38 -0.6 2,892,317 - Pf1N1B4_2548 0.38 -2.2 2,892,317 - Pf1N1B4_2548 0.38 -2.6 2,892,396 + Pf1N1B4_2548 0.45 -3.2 2,892,397 - Pf1N1B4_2548 0.45 -2.5 2,892,816 + Pf1N1B4_2548 0.85 -2.3 2,892,816 + Pf1N1B4_2548 0.85 -1.6 2,892,817 - Pf1N1B4_2548 0.85 -1.3 2,892,835 + Pf1N1B4_2548 0.87 -1.8 2,892,835 + Pf1N1B4_2548 0.87 -1.3 2,892,836 - Pf1N1B4_2548 0.87 -0.8 2,892,939 + -1.9 2,892,948 - -3.7 2,892,988 + -2.2 2,892,995 + -1.5 2,892,996 - -1.6 2,893,269 + Pf1N1B4_2549 0.36 -2.2 2,893,269 + Pf1N1B4_2549 0.36 +0.4 2,893,269 + Pf1N1B4_2549 0.36 -0.8 2,893,269 + Pf1N1B4_2549 0.36 -2.0 2,893,270 - Pf1N1B4_2549 0.36 -1.0 2,893,270 - Pf1N1B4_2549 0.36 -0.5 2,893,270 - Pf1N1B4_2549 0.36 -3.7 2,893,290 + Pf1N1B4_2549 0.38 -1.8 2,893,291 - Pf1N1B4_2549 0.38 -2.2 2,893,291 - Pf1N1B4_2549 0.38 -3.1 2,893,338 + Pf1N1B4_2549 0.44 -3.2 2,893,450 + Pf1N1B4_2549 0.57 -1.8 2,893,451 - Pf1N1B4_2549 0.58 -0.0 2,893,451 - Pf1N1B4_2549 0.58 -1.8 2,893,451 - Pf1N1B4_2549 0.58 +0.1 2,893,630 + Pf1N1B4_2549 0.79 -1.5 2,893,630 + Pf1N1B4_2549 0.79 -3.1 2,893,630 + Pf1N1B4_2549 0.79 +1.0 2,893,630 + Pf1N1B4_2549 0.79 -2.0 2,893,630 + Pf1N1B4_2549 0.79 -2.5 2,893,630 + Pf1N1B4_2549 0.79 -0.0 2,893,630 + Pf1N1B4_2549 0.79 -1.6 2,893,630 + Pf1N1B4_2549 0.79 -2.8 2,893,631 - Pf1N1B4_2549 0.79 -2.0 2,893,634 + Pf1N1B4_2549 0.79 -2.9 2,893,635 - Pf1N1B4_2549 0.80 -4.0 2,893,692 + Pf1N1B4_2549 0.86 -1.6 2,893,692 + Pf1N1B4_2549 0.86 -1.5 2,893,692 + Pf1N1B4_2549 0.86 -1.8 2,893,692 + Pf1N1B4_2549 0.86 -2.4 2,893,692 + Pf1N1B4_2549 0.86 -0.9 2,893,692 + Pf1N1B4_2549 0.86 -2.3 2,893,692 + Pf1N1B4_2549 0.86 -3.3 2,893,692 + Pf1N1B4_2549 0.86 -2.8 2,893,693 - Pf1N1B4_2549 0.86 -3.2 2,893,693 - Pf1N1B4_2549 0.86 -0.6 2,893,693 - Pf1N1B4_2549 0.86 -2.0 2,893,693 - Pf1N1B4_2549 0.86 -3.2 2,893,793 + -1.2 2,893,793 + -0.6 2,893,793 + -0.8 2,893,793 + +1.0 2,893,794 - +0.7 2,893,851 + -0.0 2,893,851 + -0.6 2,893,852 - +2.0 2,893,915 + +1.2 2,893,915 + -0.4 2,893,916 - +0.2 2,893,941 + -0.9 2,893,942 - +1.1 2,893,953 + -1.2 2,893,953 + -1.6 2,893,953 + -0.2 2,893,954 - +1.3 2,893,954 - -1.3 2,893,954 - +0.3 2,894,157 - Pf1N1B4_2550 0.29 -0.0 2,894,224 + Pf1N1B4_2550 0.38 +0.3 2,894,224 + Pf1N1B4_2550 0.38 -0.0 2,894,225 - Pf1N1B4_2550 0.39 -0.3 2,894,225 - Pf1N1B4_2550 0.39 -0.1 2,894,303 + Pf1N1B4_2550 0.50 -0.3 2,894,518 - Pf1N1B4_2550 0.80 -0.7 2,894,653 + +1.5 2,894,653 + -0.5 2,894,653 + +0.1 2,894,653 + +0.5 2,894,654 - -1.4 2,894,654 - +0.5
Or see this region's nucleotide sequence