Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2490

Experiment: D-Gluconic Acid (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2488 and Pf1N1B4_2489 overlap by 8 nucleotidesPf1N1B4_2489 and Pf1N1B4_2490 overlap by 8 nucleotidesPf1N1B4_2490 and Pf1N1B4_2491 overlap by 4 nucleotidesPf1N1B4_2491 and Pf1N1B4_2492 are separated by 120 nucleotides Pf1N1B4_2488 - 8-amino-7-oxononanoate synthase (EC 2.3.1.47), at 2,821,181 to 2,822,359 _2488 Pf1N1B4_2489 - Biotin synthesis protein BioH, at 2,822,352 to 2,823,083 _2489 Pf1N1B4_2490 - Biotin synthesis protein BioC, at 2,823,076 to 2,823,888 _2490 Pf1N1B4_2491 - Dethiobiotin synthetase (EC 6.3.3.3), at 2,823,885 to 2,824,565 _2491 Pf1N1B4_2492 - FIG00954771: hypothetical protein, at 2,824,686 to 2,824,982 _2492 Position (kb) 2823 2824Strain fitness (log2 ratio) -2 -1 0 1at 2822.298 kb on + strandat 2822.298 kb on + strandat 2822.299 kb on - strandat 2822.299 kb on - strandat 2822.305 kb on - strandat 2822.305 kb on - strandat 2822.305 kb on - strandat 2822.305 kb on - strandat 2822.305 kb on - strandat 2822.305 kb on - strandat 2822.813 kb on + strand, within Pf1N1B4_2489at 2822.813 kb on + strand, within Pf1N1B4_2489at 2822.813 kb on + strandat 2822.813 kb on + strand, within Pf1N1B4_2489at 2822.813 kb on + strand, within Pf1N1B4_2489at 2822.813 kb on + strand, within Pf1N1B4_2489at 2822.813 kb on + strand, within Pf1N1B4_2489at 2822.813 kb on + strand, within Pf1N1B4_2489at 2822.813 kb on + strand, within Pf1N1B4_2489at 2822.813 kb on + strand, within Pf1N1B4_2489at 2822.813 kb on + strand, within Pf1N1B4_2489at 2822.814 kb on - strand, within Pf1N1B4_2489at 2823.070 kb on + strandat 2823.070 kb on + strandat 2823.071 kb on - strandat 2823.071 kb on - strandat 2823.086 kb on + strandat 2823.086 kb on + strandat 2823.087 kb on - strandat 2823.163 kb on + strand, within Pf1N1B4_2490at 2823.163 kb on + strand, within Pf1N1B4_2490at 2823.163 kb on + strand, within Pf1N1B4_2490at 2823.163 kb on + strand, within Pf1N1B4_2490at 2823.164 kb on - strand, within Pf1N1B4_2490at 2823.209 kb on + strand, within Pf1N1B4_2490at 2823.209 kb on + strand, within Pf1N1B4_2490at 2823.209 kb on + strand, within Pf1N1B4_2490at 2823.210 kb on - strand, within Pf1N1B4_2490at 2823.210 kb on - strand, within Pf1N1B4_2490at 2823.210 kb on - strand, within Pf1N1B4_2490at 2823.531 kb on + strand, within Pf1N1B4_2490at 2823.532 kb on - strand, within Pf1N1B4_2490at 2823.542 kb on + strand, within Pf1N1B4_2490at 2823.543 kb on - strand, within Pf1N1B4_2490at 2823.543 kb on - strand, within Pf1N1B4_2490at 2823.543 kb on - strand, within Pf1N1B4_2490at 2823.543 kb on - strand, within Pf1N1B4_2490at 2823.553 kb on + strand, within Pf1N1B4_2490at 2823.554 kb on - strand, within Pf1N1B4_2490at 2823.554 kb on - strand, within Pf1N1B4_2490at 2823.554 kb on - strand, within Pf1N1B4_2490at 2823.625 kb on + strand, within Pf1N1B4_2490at 2823.626 kb on - strand, within Pf1N1B4_2490at 2823.626 kb on - strand, within Pf1N1B4_2490at 2823.694 kb on + strand, within Pf1N1B4_2490at 2823.811 kb on + strandat 2823.811 kb on + strandat 2823.812 kb on - strandat 2823.812 kb on - strandat 2823.812 kb on - strandat 2823.850 kb on + strandat 2823.850 kb on + strandat 2823.850 kb on + strandat 2823.851 kb on - strandat 2823.884 kb on + strandat 2823.885 kb on - strandat 2823.885 kb on - strandat 2823.897 kb on + strandat 2823.897 kb on + strandat 2823.897 kb on + strandat 2823.897 kb on + strandat 2823.897 kb on + strandat 2823.898 kb on - strandat 2823.898 kb on - strandat 2823.898 kb on - strandat 2824.089 kb on + strand, within Pf1N1B4_2491at 2824.089 kb on + strand, within Pf1N1B4_2491at 2824.089 kb on + strand, within Pf1N1B4_2491at 2824.089 kb on + strand, within Pf1N1B4_2491at 2824.090 kb on - strand, within Pf1N1B4_2491at 2824.090 kb on - strand, within Pf1N1B4_2491at 2824.090 kb on - strand, within Pf1N1B4_2491at 2824.090 kb on - strand, within Pf1N1B4_2491at 2824.090 kb on - strand, within Pf1N1B4_2491at 2824.583 kb on + strandat 2824.583 kb on + strandat 2824.583 kb on + strandat 2824.730 kb on + strand, within Pf1N1B4_2492at 2824.730 kb on + strand, within Pf1N1B4_2492at 2824.880 kb on + strand, within Pf1N1B4_2492at 2824.880 kb on + strand, within Pf1N1B4_2492at 2824.881 kb on - strand, within Pf1N1B4_2492at 2824.881 kb on - strand, within Pf1N1B4_2492at 2824.881 kb on - strand, within Pf1N1B4_2492

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Gluconic Acid (C)
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2,822,298 + -1.8
2,822,298 + -1.2
2,822,299 - -0.4
2,822,299 - -0.0
2,822,305 - -2.2
2,822,305 - -0.9
2,822,305 - +0.1
2,822,305 - -1.4
2,822,305 - -1.3
2,822,305 - +0.2
2,822,813 + Pf1N1B4_2489 0.63 +0.6
2,822,813 + Pf1N1B4_2489 0.63 -1.1
2,822,813 + +0.6
2,822,813 + Pf1N1B4_2489 0.63 -1.6
2,822,813 + Pf1N1B4_2489 0.63 -0.2
2,822,813 + Pf1N1B4_2489 0.63 +1.0
2,822,813 + Pf1N1B4_2489 0.63 -0.6
2,822,813 + Pf1N1B4_2489 0.63 +0.2
2,822,813 + Pf1N1B4_2489 0.63 -0.0
2,822,813 + Pf1N1B4_2489 0.63 -1.2
2,822,813 + Pf1N1B4_2489 0.63 -1.6
2,822,814 - Pf1N1B4_2489 0.63 +0.3
2,823,070 + -1.3
2,823,070 + +1.0
2,823,071 - -1.8
2,823,071 - -0.4
2,823,086 + +0.7
2,823,086 + -0.1
2,823,087 - -1.0
2,823,163 + Pf1N1B4_2490 0.11 +0.3
2,823,163 + Pf1N1B4_2490 0.11 -1.4
2,823,163 + Pf1N1B4_2490 0.11 +0.6
2,823,163 + Pf1N1B4_2490 0.11 -0.4
2,823,164 - Pf1N1B4_2490 0.11 -1.0
2,823,209 + Pf1N1B4_2490 0.16 -0.9
2,823,209 + Pf1N1B4_2490 0.16 -1.4
2,823,209 + Pf1N1B4_2490 0.16 -1.9
2,823,210 - Pf1N1B4_2490 0.16 -0.6
2,823,210 - Pf1N1B4_2490 0.16 -1.0
2,823,210 - Pf1N1B4_2490 0.16 -1.3
2,823,531 + Pf1N1B4_2490 0.56 +0.7
2,823,532 - Pf1N1B4_2490 0.56 -0.8
2,823,542 + Pf1N1B4_2490 0.57 -0.5
2,823,543 - Pf1N1B4_2490 0.57 -0.6
2,823,543 - Pf1N1B4_2490 0.57 +0.8
2,823,543 - Pf1N1B4_2490 0.57 -1.4
2,823,543 - Pf1N1B4_2490 0.57 -1.4
2,823,553 + Pf1N1B4_2490 0.59 +0.4
2,823,554 - Pf1N1B4_2490 0.59 -0.8
2,823,554 - Pf1N1B4_2490 0.59 -0.8
2,823,554 - Pf1N1B4_2490 0.59 -1.3
2,823,625 + Pf1N1B4_2490 0.68 -1.9
2,823,626 - Pf1N1B4_2490 0.68 -0.7
2,823,626 - Pf1N1B4_2490 0.68 +0.3
2,823,694 + Pf1N1B4_2490 0.76 -0.0
2,823,811 + -0.4
2,823,811 + -0.4
2,823,812 - -1.0
2,823,812 - -2.3
2,823,812 - -0.4
2,823,850 + -1.1
2,823,850 + +1.6
2,823,850 + -1.0
2,823,851 - -1.2
2,823,884 + -0.2
2,823,885 - +0.2
2,823,885 - -0.0
2,823,897 + +1.6
2,823,897 + -0.2
2,823,897 + -0.0
2,823,897 + -0.2
2,823,897 + +1.3
2,823,898 - -1.2
2,823,898 - +0.1
2,823,898 - -1.0
2,824,089 + Pf1N1B4_2491 0.30 -1.2
2,824,089 + Pf1N1B4_2491 0.30 -0.8
2,824,089 + Pf1N1B4_2491 0.30 -1.1
2,824,089 + Pf1N1B4_2491 0.30 -1.8
2,824,090 - Pf1N1B4_2491 0.30 -0.8
2,824,090 - Pf1N1B4_2491 0.30 -0.2
2,824,090 - Pf1N1B4_2491 0.30 -1.4
2,824,090 - Pf1N1B4_2491 0.30 -1.2
2,824,090 - Pf1N1B4_2491 0.30 -1.2
2,824,583 + -0.0
2,824,583 + -0.2
2,824,583 + -0.9
2,824,730 + Pf1N1B4_2492 0.15 +0.3
2,824,730 + Pf1N1B4_2492 0.15 +1.6
2,824,880 + Pf1N1B4_2492 0.65 -0.4
2,824,880 + Pf1N1B4_2492 0.65 -0.2
2,824,881 - Pf1N1B4_2492 0.66 -0.6
2,824,881 - Pf1N1B4_2492 0.66 -0.7
2,824,881 - Pf1N1B4_2492 0.66 +0.2

Or see this region's nucleotide sequence