Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2488

Experiment: D-Gluconic Acid (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2487 and Pf1N1B4_2488 are separated by 186 nucleotidesPf1N1B4_2488 and Pf1N1B4_2489 overlap by 8 nucleotidesPf1N1B4_2489 and Pf1N1B4_2490 overlap by 8 nucleotides Pf1N1B4_2487 - Biotin synthase (EC 2.8.1.6), at 2,819,939 to 2,820,994 _2487 Pf1N1B4_2488 - 8-amino-7-oxononanoate synthase (EC 2.3.1.47), at 2,821,181 to 2,822,359 _2488 Pf1N1B4_2489 - Biotin synthesis protein BioH, at 2,822,352 to 2,823,083 _2489 Pf1N1B4_2490 - Biotin synthesis protein BioC, at 2,823,076 to 2,823,888 _2490 Position (kb) 2821 2822 2823Strain fitness (log2 ratio) -3 -2 -1 0 1at 2820.290 kb on + strand, within Pf1N1B4_2487at 2820.291 kb on - strand, within Pf1N1B4_2487at 2820.401 kb on + strand, within Pf1N1B4_2487at 2820.401 kb on + strand, within Pf1N1B4_2487at 2820.402 kb on - strandat 2820.404 kb on + strand, within Pf1N1B4_2487at 2820.404 kb on + strand, within Pf1N1B4_2487at 2820.404 kb on + strand, within Pf1N1B4_2487at 2820.404 kb on + strand, within Pf1N1B4_2487at 2820.404 kb on + strand, within Pf1N1B4_2487at 2820.404 kb on + strand, within Pf1N1B4_2487at 2820.405 kb on - strand, within Pf1N1B4_2487at 2820.405 kb on - strand, within Pf1N1B4_2487at 2820.405 kb on - strand, within Pf1N1B4_2487at 2820.405 kb on - strand, within Pf1N1B4_2487at 2820.405 kb on - strand, within Pf1N1B4_2487at 2820.437 kb on + strand, within Pf1N1B4_2487at 2820.437 kb on + strand, within Pf1N1B4_2487at 2820.437 kb on + strand, within Pf1N1B4_2487at 2820.438 kb on - strand, within Pf1N1B4_2487at 2820.460 kb on + strand, within Pf1N1B4_2487at 2820.460 kb on + strand, within Pf1N1B4_2487at 2820.460 kb on + strand, within Pf1N1B4_2487at 2820.460 kb on + strand, within Pf1N1B4_2487at 2820.464 kb on + strand, within Pf1N1B4_2487at 2820.464 kb on + strand, within Pf1N1B4_2487at 2820.491 kb on + strand, within Pf1N1B4_2487at 2820.491 kb on + strand, within Pf1N1B4_2487at 2820.491 kb on + strand, within Pf1N1B4_2487at 2820.491 kb on + strand, within Pf1N1B4_2487at 2820.491 kb on + strand, within Pf1N1B4_2487at 2820.491 kb on + strand, within Pf1N1B4_2487at 2820.491 kb on + strand, within Pf1N1B4_2487at 2820.492 kb on - strand, within Pf1N1B4_2487at 2820.977 kb on + strandat 2820.977 kb on + strandat 2820.977 kb on + strandat 2820.977 kb on + strandat 2820.977 kb on + strandat 2820.977 kb on + strandat 2820.977 kb on + strandat 2820.978 kb on - strandat 2820.978 kb on - strandat 2820.978 kb on - strandat 2820.978 kb on - strandat 2820.978 kb on - strandat 2821.002 kb on - strandat 2821.002 kb on - strandat 2821.002 kb on - strandat 2821.138 kb on - strandat 2821.138 kb on - strandat 2821.138 kb on - strandat 2821.138 kb on - strandat 2821.138 kb on - strandat 2821.233 kb on - strandat 2821.309 kb on + strand, within Pf1N1B4_2488at 2821.309 kb on + strand, within Pf1N1B4_2488at 2821.309 kb on + strand, within Pf1N1B4_2488at 2821.319 kb on + strand, within Pf1N1B4_2488at 2821.320 kb on - strand, within Pf1N1B4_2488at 2821.499 kb on + strand, within Pf1N1B4_2488at 2821.500 kb on - strand, within Pf1N1B4_2488at 2821.500 kb on - strand, within Pf1N1B4_2488at 2821.500 kb on - strand, within Pf1N1B4_2488at 2821.500 kb on - strand, within Pf1N1B4_2488at 2821.679 kb on - strand, within Pf1N1B4_2488at 2821.964 kb on + strand, within Pf1N1B4_2488at 2821.964 kb on + strand, within Pf1N1B4_2488at 2821.964 kb on + strand, within Pf1N1B4_2488at 2821.965 kb on - strand, within Pf1N1B4_2488at 2821.965 kb on - strand, within Pf1N1B4_2488at 2821.965 kb on - strand, within Pf1N1B4_2488at 2821.965 kb on - strand, within Pf1N1B4_2488at 2821.965 kb on - strand, within Pf1N1B4_2488at 2821.965 kb on - strand, within Pf1N1B4_2488at 2821.970 kb on + strand, within Pf1N1B4_2488at 2821.970 kb on + strand, within Pf1N1B4_2488at 2821.970 kb on + strand, within Pf1N1B4_2488at 2821.970 kb on + strand, within Pf1N1B4_2488at 2821.970 kb on + strand, within Pf1N1B4_2488at 2821.971 kb on - strand, within Pf1N1B4_2488at 2821.971 kb on - strand, within Pf1N1B4_2488at 2821.971 kb on - strand, within Pf1N1B4_2488at 2821.971 kb on - strand, within Pf1N1B4_2488at 2821.971 kb on - strand, within Pf1N1B4_2488at 2821.971 kb on - strand, within Pf1N1B4_2488at 2821.971 kb on - strand, within Pf1N1B4_2488at 2822.298 kb on + strandat 2822.298 kb on + strandat 2822.299 kb on - strandat 2822.299 kb on - strandat 2822.305 kb on - strandat 2822.305 kb on - strandat 2822.305 kb on - strandat 2822.305 kb on - strandat 2822.305 kb on - strandat 2822.305 kb on - strandat 2822.813 kb on + strand, within Pf1N1B4_2489at 2822.813 kb on + strand, within Pf1N1B4_2489at 2822.813 kb on + strandat 2822.813 kb on + strand, within Pf1N1B4_2489at 2822.813 kb on + strand, within Pf1N1B4_2489at 2822.813 kb on + strand, within Pf1N1B4_2489at 2822.813 kb on + strand, within Pf1N1B4_2489at 2822.813 kb on + strand, within Pf1N1B4_2489at 2822.813 kb on + strand, within Pf1N1B4_2489at 2822.813 kb on + strand, within Pf1N1B4_2489at 2822.813 kb on + strand, within Pf1N1B4_2489at 2822.814 kb on - strand, within Pf1N1B4_2489at 2823.070 kb on + strandat 2823.070 kb on + strandat 2823.071 kb on - strandat 2823.071 kb on - strandat 2823.086 kb on + strandat 2823.086 kb on + strandat 2823.087 kb on - strandat 2823.163 kb on + strand, within Pf1N1B4_2490at 2823.163 kb on + strand, within Pf1N1B4_2490at 2823.163 kb on + strand, within Pf1N1B4_2490at 2823.163 kb on + strand, within Pf1N1B4_2490at 2823.164 kb on - strand, within Pf1N1B4_2490at 2823.209 kb on + strand, within Pf1N1B4_2490at 2823.209 kb on + strand, within Pf1N1B4_2490at 2823.209 kb on + strand, within Pf1N1B4_2490at 2823.210 kb on - strand, within Pf1N1B4_2490at 2823.210 kb on - strand, within Pf1N1B4_2490at 2823.210 kb on - strand, within Pf1N1B4_2490

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Gluconic Acid (C)
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2,820,290 + Pf1N1B4_2487 0.33 -0.4
2,820,291 - Pf1N1B4_2487 0.33 -0.0
2,820,401 + Pf1N1B4_2487 0.44 +1.0
2,820,401 + Pf1N1B4_2487 0.44 +0.6
2,820,402 - -0.4
2,820,404 + Pf1N1B4_2487 0.44 -1.2
2,820,404 + Pf1N1B4_2487 0.44 +0.6
2,820,404 + Pf1N1B4_2487 0.44 -1.4
2,820,404 + Pf1N1B4_2487 0.44 -0.2
2,820,404 + Pf1N1B4_2487 0.44 +0.3
2,820,404 + Pf1N1B4_2487 0.44 -1.0
2,820,405 - Pf1N1B4_2487 0.44 -1.6
2,820,405 - Pf1N1B4_2487 0.44 -0.9
2,820,405 - Pf1N1B4_2487 0.44 -1.4
2,820,405 - Pf1N1B4_2487 0.44 -1.0
2,820,405 - Pf1N1B4_2487 0.44 -2.5
2,820,437 + Pf1N1B4_2487 0.47 -0.2
2,820,437 + Pf1N1B4_2487 0.47 -1.4
2,820,437 + Pf1N1B4_2487 0.47 +0.6
2,820,438 - Pf1N1B4_2487 0.47 -0.0
2,820,460 + Pf1N1B4_2487 0.49 -1.0
2,820,460 + Pf1N1B4_2487 0.49 -2.1
2,820,460 + Pf1N1B4_2487 0.49 -0.8
2,820,460 + Pf1N1B4_2487 0.49 +1.1
2,820,464 + Pf1N1B4_2487 0.50 -1.2
2,820,464 + Pf1N1B4_2487 0.50 +0.3
2,820,491 + Pf1N1B4_2487 0.52 -2.4
2,820,491 + Pf1N1B4_2487 0.52 -0.7
2,820,491 + Pf1N1B4_2487 0.52 -2.4
2,820,491 + Pf1N1B4_2487 0.52 +0.2
2,820,491 + Pf1N1B4_2487 0.52 -1.7
2,820,491 + Pf1N1B4_2487 0.52 -1.2
2,820,491 + Pf1N1B4_2487 0.52 -1.3
2,820,492 - Pf1N1B4_2487 0.52 -1.4
2,820,977 + -1.2
2,820,977 + +0.7
2,820,977 + +0.6
2,820,977 + -0.4
2,820,977 + +1.0
2,820,977 + -1.2
2,820,977 + -0.0
2,820,978 - -1.2
2,820,978 - -1.2
2,820,978 - -1.0
2,820,978 - -0.8
2,820,978 - -0.4
2,821,002 - -1.0
2,821,002 - -0.4
2,821,002 - -1.3
2,821,138 - -1.1
2,821,138 - -1.6
2,821,138 - -2.6
2,821,138 - -1.8
2,821,138 - -0.0
2,821,233 - -1.1
2,821,309 + Pf1N1B4_2488 0.11 -3.0
2,821,309 + Pf1N1B4_2488 0.11 -0.0
2,821,309 + Pf1N1B4_2488 0.11 -1.0
2,821,319 + Pf1N1B4_2488 0.12 -0.8
2,821,320 - Pf1N1B4_2488 0.12 -1.1
2,821,499 + Pf1N1B4_2488 0.27 -1.0
2,821,500 - Pf1N1B4_2488 0.27 -1.2
2,821,500 - Pf1N1B4_2488 0.27 +0.6
2,821,500 - Pf1N1B4_2488 0.27 -2.2
2,821,500 - Pf1N1B4_2488 0.27 -1.4
2,821,679 - Pf1N1B4_2488 0.42 -1.0
2,821,964 + Pf1N1B4_2488 0.66 -1.0
2,821,964 + Pf1N1B4_2488 0.66 -0.0
2,821,964 + Pf1N1B4_2488 0.66 -1.2
2,821,965 - Pf1N1B4_2488 0.66 -1.2
2,821,965 - Pf1N1B4_2488 0.66 -1.0
2,821,965 - Pf1N1B4_2488 0.66 -2.1
2,821,965 - Pf1N1B4_2488 0.66 -0.2
2,821,965 - Pf1N1B4_2488 0.66 -2.3
2,821,965 - Pf1N1B4_2488 0.66 -2.1
2,821,970 + Pf1N1B4_2488 0.67 -0.1
2,821,970 + Pf1N1B4_2488 0.67 -1.0
2,821,970 + Pf1N1B4_2488 0.67 -0.8
2,821,970 + Pf1N1B4_2488 0.67 -0.8
2,821,970 + Pf1N1B4_2488 0.67 -1.0
2,821,971 - Pf1N1B4_2488 0.67 -0.9
2,821,971 - Pf1N1B4_2488 0.67 -2.0
2,821,971 - Pf1N1B4_2488 0.67 -0.4
2,821,971 - Pf1N1B4_2488 0.67 -0.0
2,821,971 - Pf1N1B4_2488 0.67 -1.8
2,821,971 - Pf1N1B4_2488 0.67 -0.8
2,821,971 - Pf1N1B4_2488 0.67 -0.9
2,822,298 + -1.8
2,822,298 + -1.2
2,822,299 - -0.4
2,822,299 - -0.0
2,822,305 - -2.2
2,822,305 - -0.9
2,822,305 - +0.1
2,822,305 - -1.4
2,822,305 - -1.3
2,822,305 - +0.2
2,822,813 + Pf1N1B4_2489 0.63 +0.6
2,822,813 + Pf1N1B4_2489 0.63 -1.1
2,822,813 + +0.6
2,822,813 + Pf1N1B4_2489 0.63 -1.6
2,822,813 + Pf1N1B4_2489 0.63 -0.2
2,822,813 + Pf1N1B4_2489 0.63 +1.0
2,822,813 + Pf1N1B4_2489 0.63 -0.6
2,822,813 + Pf1N1B4_2489 0.63 +0.2
2,822,813 + Pf1N1B4_2489 0.63 -0.0
2,822,813 + Pf1N1B4_2489 0.63 -1.2
2,822,813 + Pf1N1B4_2489 0.63 -1.6
2,822,814 - Pf1N1B4_2489 0.63 +0.3
2,823,070 + -1.3
2,823,070 + +1.0
2,823,071 - -1.8
2,823,071 - -0.4
2,823,086 + +0.7
2,823,086 + -0.1
2,823,087 - -1.0
2,823,163 + Pf1N1B4_2490 0.11 +0.3
2,823,163 + Pf1N1B4_2490 0.11 -1.4
2,823,163 + Pf1N1B4_2490 0.11 +0.6
2,823,163 + Pf1N1B4_2490 0.11 -0.4
2,823,164 - Pf1N1B4_2490 0.11 -1.0
2,823,209 + Pf1N1B4_2490 0.16 -0.9
2,823,209 + Pf1N1B4_2490 0.16 -1.4
2,823,209 + Pf1N1B4_2490 0.16 -1.9
2,823,210 - Pf1N1B4_2490 0.16 -0.6
2,823,210 - Pf1N1B4_2490 0.16 -1.0
2,823,210 - Pf1N1B4_2490 0.16 -1.3

Or see this region's nucleotide sequence