Experiment: D-Gluconic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2487 and Pf1N1B4_2488 are separated by 186 nucleotides Pf1N1B4_2488 and Pf1N1B4_2489 overlap by 8 nucleotides Pf1N1B4_2489 and Pf1N1B4_2490 overlap by 8 nucleotides
Pf1N1B4_2487 - Biotin synthase (EC 2.8.1.6), at 2,819,939 to 2,820,994
_2487
Pf1N1B4_2488 - 8-amino-7-oxononanoate synthase (EC 2.3.1.47), at 2,821,181 to 2,822,359
_2488
Pf1N1B4_2489 - Biotin synthesis protein BioH, at 2,822,352 to 2,823,083
_2489
Pf1N1B4_2490 - Biotin synthesis protein BioC, at 2,823,076 to 2,823,888
_2490
Position (kb)
2821
2822
2823 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2820.290 kb on + strand, within Pf1N1B4_2487 at 2820.291 kb on - strand, within Pf1N1B4_2487 at 2820.401 kb on + strand, within Pf1N1B4_2487 at 2820.401 kb on + strand, within Pf1N1B4_2487 at 2820.402 kb on - strand at 2820.404 kb on + strand, within Pf1N1B4_2487 at 2820.404 kb on + strand, within Pf1N1B4_2487 at 2820.404 kb on + strand, within Pf1N1B4_2487 at 2820.404 kb on + strand, within Pf1N1B4_2487 at 2820.404 kb on + strand, within Pf1N1B4_2487 at 2820.404 kb on + strand, within Pf1N1B4_2487 at 2820.405 kb on - strand, within Pf1N1B4_2487 at 2820.405 kb on - strand, within Pf1N1B4_2487 at 2820.405 kb on - strand, within Pf1N1B4_2487 at 2820.405 kb on - strand, within Pf1N1B4_2487 at 2820.405 kb on - strand, within Pf1N1B4_2487 at 2820.437 kb on + strand, within Pf1N1B4_2487 at 2820.437 kb on + strand, within Pf1N1B4_2487 at 2820.437 kb on + strand, within Pf1N1B4_2487 at 2820.438 kb on - strand, within Pf1N1B4_2487 at 2820.460 kb on + strand, within Pf1N1B4_2487 at 2820.460 kb on + strand, within Pf1N1B4_2487 at 2820.460 kb on + strand, within Pf1N1B4_2487 at 2820.460 kb on + strand, within Pf1N1B4_2487 at 2820.464 kb on + strand, within Pf1N1B4_2487 at 2820.464 kb on + strand, within Pf1N1B4_2487 at 2820.491 kb on + strand, within Pf1N1B4_2487 at 2820.491 kb on + strand, within Pf1N1B4_2487 at 2820.491 kb on + strand, within Pf1N1B4_2487 at 2820.491 kb on + strand, within Pf1N1B4_2487 at 2820.491 kb on + strand, within Pf1N1B4_2487 at 2820.491 kb on + strand, within Pf1N1B4_2487 at 2820.491 kb on + strand, within Pf1N1B4_2487 at 2820.492 kb on - strand, within Pf1N1B4_2487 at 2820.977 kb on + strand at 2820.977 kb on + strand at 2820.977 kb on + strand at 2820.977 kb on + strand at 2820.977 kb on + strand at 2820.977 kb on + strand at 2820.977 kb on + strand at 2820.978 kb on - strand at 2820.978 kb on - strand at 2820.978 kb on - strand at 2820.978 kb on - strand at 2820.978 kb on - strand at 2821.002 kb on - strand at 2821.002 kb on - strand at 2821.002 kb on - strand at 2821.138 kb on - strand at 2821.138 kb on - strand at 2821.138 kb on - strand at 2821.138 kb on - strand at 2821.138 kb on - strand at 2821.233 kb on - strand at 2821.309 kb on + strand, within Pf1N1B4_2488 at 2821.309 kb on + strand, within Pf1N1B4_2488 at 2821.309 kb on + strand, within Pf1N1B4_2488 at 2821.319 kb on + strand, within Pf1N1B4_2488 at 2821.320 kb on - strand, within Pf1N1B4_2488 at 2821.499 kb on + strand, within Pf1N1B4_2488 at 2821.500 kb on - strand, within Pf1N1B4_2488 at 2821.500 kb on - strand, within Pf1N1B4_2488 at 2821.500 kb on - strand, within Pf1N1B4_2488 at 2821.500 kb on - strand, within Pf1N1B4_2488 at 2821.679 kb on - strand, within Pf1N1B4_2488 at 2821.964 kb on + strand, within Pf1N1B4_2488 at 2821.964 kb on + strand, within Pf1N1B4_2488 at 2821.964 kb on + strand, within Pf1N1B4_2488 at 2821.965 kb on - strand, within Pf1N1B4_2488 at 2821.965 kb on - strand, within Pf1N1B4_2488 at 2821.965 kb on - strand, within Pf1N1B4_2488 at 2821.965 kb on - strand, within Pf1N1B4_2488 at 2821.965 kb on - strand, within Pf1N1B4_2488 at 2821.965 kb on - strand, within Pf1N1B4_2488 at 2821.970 kb on + strand, within Pf1N1B4_2488 at 2821.970 kb on + strand, within Pf1N1B4_2488 at 2821.970 kb on + strand, within Pf1N1B4_2488 at 2821.970 kb on + strand, within Pf1N1B4_2488 at 2821.970 kb on + strand, within Pf1N1B4_2488 at 2821.971 kb on - strand, within Pf1N1B4_2488 at 2821.971 kb on - strand, within Pf1N1B4_2488 at 2821.971 kb on - strand, within Pf1N1B4_2488 at 2821.971 kb on - strand, within Pf1N1B4_2488 at 2821.971 kb on - strand, within Pf1N1B4_2488 at 2821.971 kb on - strand, within Pf1N1B4_2488 at 2821.971 kb on - strand, within Pf1N1B4_2488 at 2822.298 kb on + strand at 2822.298 kb on + strand at 2822.299 kb on - strand at 2822.299 kb on - strand at 2822.305 kb on - strand at 2822.305 kb on - strand at 2822.305 kb on - strand at 2822.305 kb on - strand at 2822.305 kb on - strand at 2822.305 kb on - strand at 2822.813 kb on + strand, within Pf1N1B4_2489 at 2822.813 kb on + strand, within Pf1N1B4_2489 at 2822.813 kb on + strand at 2822.813 kb on + strand, within Pf1N1B4_2489 at 2822.813 kb on + strand, within Pf1N1B4_2489 at 2822.813 kb on + strand, within Pf1N1B4_2489 at 2822.813 kb on + strand, within Pf1N1B4_2489 at 2822.813 kb on + strand, within Pf1N1B4_2489 at 2822.813 kb on + strand, within Pf1N1B4_2489 at 2822.813 kb on + strand, within Pf1N1B4_2489 at 2822.813 kb on + strand, within Pf1N1B4_2489 at 2822.814 kb on - strand, within Pf1N1B4_2489 at 2823.070 kb on + strand at 2823.070 kb on + strand at 2823.071 kb on - strand at 2823.071 kb on - strand at 2823.086 kb on + strand at 2823.086 kb on + strand at 2823.087 kb on - strand at 2823.163 kb on + strand, within Pf1N1B4_2490 at 2823.163 kb on + strand, within Pf1N1B4_2490 at 2823.163 kb on + strand, within Pf1N1B4_2490 at 2823.163 kb on + strand, within Pf1N1B4_2490 at 2823.164 kb on - strand, within Pf1N1B4_2490 at 2823.209 kb on + strand, within Pf1N1B4_2490 at 2823.209 kb on + strand, within Pf1N1B4_2490 at 2823.209 kb on + strand, within Pf1N1B4_2490 at 2823.210 kb on - strand, within Pf1N1B4_2490 at 2823.210 kb on - strand, within Pf1N1B4_2490 at 2823.210 kb on - strand, within Pf1N1B4_2490
Per-strain Table
Position Strand Gene LocusTag Fraction D-Gluconic Acid (C) remove 2,820,290 + Pf1N1B4_2487 0.33 -0.4 2,820,291 - Pf1N1B4_2487 0.33 -0.0 2,820,401 + Pf1N1B4_2487 0.44 +1.0 2,820,401 + Pf1N1B4_2487 0.44 +0.6 2,820,402 - -0.4 2,820,404 + Pf1N1B4_2487 0.44 -1.2 2,820,404 + Pf1N1B4_2487 0.44 +0.6 2,820,404 + Pf1N1B4_2487 0.44 -1.4 2,820,404 + Pf1N1B4_2487 0.44 -0.2 2,820,404 + Pf1N1B4_2487 0.44 +0.3 2,820,404 + Pf1N1B4_2487 0.44 -1.0 2,820,405 - Pf1N1B4_2487 0.44 -1.6 2,820,405 - Pf1N1B4_2487 0.44 -0.9 2,820,405 - Pf1N1B4_2487 0.44 -1.4 2,820,405 - Pf1N1B4_2487 0.44 -1.0 2,820,405 - Pf1N1B4_2487 0.44 -2.5 2,820,437 + Pf1N1B4_2487 0.47 -0.2 2,820,437 + Pf1N1B4_2487 0.47 -1.4 2,820,437 + Pf1N1B4_2487 0.47 +0.6 2,820,438 - Pf1N1B4_2487 0.47 -0.0 2,820,460 + Pf1N1B4_2487 0.49 -1.0 2,820,460 + Pf1N1B4_2487 0.49 -2.1 2,820,460 + Pf1N1B4_2487 0.49 -0.8 2,820,460 + Pf1N1B4_2487 0.49 +1.1 2,820,464 + Pf1N1B4_2487 0.50 -1.2 2,820,464 + Pf1N1B4_2487 0.50 +0.3 2,820,491 + Pf1N1B4_2487 0.52 -2.4 2,820,491 + Pf1N1B4_2487 0.52 -0.7 2,820,491 + Pf1N1B4_2487 0.52 -2.4 2,820,491 + Pf1N1B4_2487 0.52 +0.2 2,820,491 + Pf1N1B4_2487 0.52 -1.7 2,820,491 + Pf1N1B4_2487 0.52 -1.2 2,820,491 + Pf1N1B4_2487 0.52 -1.3 2,820,492 - Pf1N1B4_2487 0.52 -1.4 2,820,977 + -1.2 2,820,977 + +0.7 2,820,977 + +0.6 2,820,977 + -0.4 2,820,977 + +1.0 2,820,977 + -1.2 2,820,977 + -0.0 2,820,978 - -1.2 2,820,978 - -1.2 2,820,978 - -1.0 2,820,978 - -0.8 2,820,978 - -0.4 2,821,002 - -1.0 2,821,002 - -0.4 2,821,002 - -1.3 2,821,138 - -1.1 2,821,138 - -1.6 2,821,138 - -2.6 2,821,138 - -1.8 2,821,138 - -0.0 2,821,233 - -1.1 2,821,309 + Pf1N1B4_2488 0.11 -3.0 2,821,309 + Pf1N1B4_2488 0.11 -0.0 2,821,309 + Pf1N1B4_2488 0.11 -1.0 2,821,319 + Pf1N1B4_2488 0.12 -0.8 2,821,320 - Pf1N1B4_2488 0.12 -1.1 2,821,499 + Pf1N1B4_2488 0.27 -1.0 2,821,500 - Pf1N1B4_2488 0.27 -1.2 2,821,500 - Pf1N1B4_2488 0.27 +0.6 2,821,500 - Pf1N1B4_2488 0.27 -2.2 2,821,500 - Pf1N1B4_2488 0.27 -1.4 2,821,679 - Pf1N1B4_2488 0.42 -1.0 2,821,964 + Pf1N1B4_2488 0.66 -1.0 2,821,964 + Pf1N1B4_2488 0.66 -0.0 2,821,964 + Pf1N1B4_2488 0.66 -1.2 2,821,965 - Pf1N1B4_2488 0.66 -1.2 2,821,965 - Pf1N1B4_2488 0.66 -1.0 2,821,965 - Pf1N1B4_2488 0.66 -2.1 2,821,965 - Pf1N1B4_2488 0.66 -0.2 2,821,965 - Pf1N1B4_2488 0.66 -2.3 2,821,965 - Pf1N1B4_2488 0.66 -2.1 2,821,970 + Pf1N1B4_2488 0.67 -0.1 2,821,970 + Pf1N1B4_2488 0.67 -1.0 2,821,970 + Pf1N1B4_2488 0.67 -0.8 2,821,970 + Pf1N1B4_2488 0.67 -0.8 2,821,970 + Pf1N1B4_2488 0.67 -1.0 2,821,971 - Pf1N1B4_2488 0.67 -0.9 2,821,971 - Pf1N1B4_2488 0.67 -2.0 2,821,971 - Pf1N1B4_2488 0.67 -0.4 2,821,971 - Pf1N1B4_2488 0.67 -0.0 2,821,971 - Pf1N1B4_2488 0.67 -1.8 2,821,971 - Pf1N1B4_2488 0.67 -0.8 2,821,971 - Pf1N1B4_2488 0.67 -0.9 2,822,298 + -1.8 2,822,298 + -1.2 2,822,299 - -0.4 2,822,299 - -0.0 2,822,305 - -2.2 2,822,305 - -0.9 2,822,305 - +0.1 2,822,305 - -1.4 2,822,305 - -1.3 2,822,305 - +0.2 2,822,813 + Pf1N1B4_2489 0.63 +0.6 2,822,813 + Pf1N1B4_2489 0.63 -1.1 2,822,813 + +0.6 2,822,813 + Pf1N1B4_2489 0.63 -1.6 2,822,813 + Pf1N1B4_2489 0.63 -0.2 2,822,813 + Pf1N1B4_2489 0.63 +1.0 2,822,813 + Pf1N1B4_2489 0.63 -0.6 2,822,813 + Pf1N1B4_2489 0.63 +0.2 2,822,813 + Pf1N1B4_2489 0.63 -0.0 2,822,813 + Pf1N1B4_2489 0.63 -1.2 2,822,813 + Pf1N1B4_2489 0.63 -1.6 2,822,814 - Pf1N1B4_2489 0.63 +0.3 2,823,070 + -1.3 2,823,070 + +1.0 2,823,071 - -1.8 2,823,071 - -0.4 2,823,086 + +0.7 2,823,086 + -0.1 2,823,087 - -1.0 2,823,163 + Pf1N1B4_2490 0.11 +0.3 2,823,163 + Pf1N1B4_2490 0.11 -1.4 2,823,163 + Pf1N1B4_2490 0.11 +0.6 2,823,163 + Pf1N1B4_2490 0.11 -0.4 2,823,164 - Pf1N1B4_2490 0.11 -1.0 2,823,209 + Pf1N1B4_2490 0.16 -0.9 2,823,209 + Pf1N1B4_2490 0.16 -1.4 2,823,209 + Pf1N1B4_2490 0.16 -1.9 2,823,210 - Pf1N1B4_2490 0.16 -0.6 2,823,210 - Pf1N1B4_2490 0.16 -1.0 2,823,210 - Pf1N1B4_2490 0.16 -1.3
Or see this region's nucleotide sequence