Experiment: D-Gluconic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2484 and Pf1N1B4_2485 overlap by 43 nucleotides Pf1N1B4_2485 and Pf1N1B4_2486 are separated by 114 nucleotides Pf1N1B4_2486 and Pf1N1B4_2487 overlap by 33 nucleotides Pf1N1B4_2487 and Pf1N1B4_2488 are separated by 186 nucleotides
Pf1N1B4_2484 - hypothetical protein, at 2,818,947 to 2,819,150
_2484
Pf1N1B4_2485 - Competence protein F homolog, phosphoribosyltransferase domain; protein YhgH required for utilization of DNA as sole source of carbon and energy, at 2,819,108 to 2,819,743
_2485
Pf1N1B4_2486 - hypothetical protein, at 2,819,858 to 2,819,971
_2486
Pf1N1B4_2487 - Biotin synthase (EC 2.8.1.6), at 2,819,939 to 2,820,994
_2487
Pf1N1B4_2488 - 8-amino-7-oxononanoate synthase (EC 2.3.1.47), at 2,821,181 to 2,822,359
_2488
Position (kb)
2819
2820
2821 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2819.033 kb on + strand, within Pf1N1B4_2484 at 2819.034 kb on - strand, within Pf1N1B4_2484 at 2819.104 kb on + strand, within Pf1N1B4_2484 at 2819.104 kb on + strand, within Pf1N1B4_2484 at 2819.104 kb on + strand at 2819.104 kb on + strand, within Pf1N1B4_2484 at 2819.105 kb on - strand, within Pf1N1B4_2484 at 2819.105 kb on - strand, within Pf1N1B4_2484 at 2819.148 kb on + strand at 2819.149 kb on - strand at 2819.604 kb on + strand, within Pf1N1B4_2485 at 2819.604 kb on + strand, within Pf1N1B4_2485 at 2819.604 kb on + strand, within Pf1N1B4_2485 at 2819.605 kb on - strand, within Pf1N1B4_2485 at 2819.605 kb on - strand, within Pf1N1B4_2485 at 2819.744 kb on - strand at 2819.783 kb on + strand at 2819.784 kb on - strand at 2819.784 kb on - strand at 2819.814 kb on + strand at 2819.826 kb on - strand at 2819.859 kb on + strand at 2819.863 kb on + strand at 2819.863 kb on + strand at 2819.863 kb on + strand at 2819.863 kb on + strand at 2819.863 kb on + strand at 2819.863 kb on + strand at 2819.863 kb on + strand at 2819.863 kb on + strand at 2819.863 kb on + strand at 2819.864 kb on - strand at 2819.864 kb on - strand at 2819.894 kb on - strand, within Pf1N1B4_2486 at 2819.922 kb on + strand, within Pf1N1B4_2486 at 2819.923 kb on - strand, within Pf1N1B4_2486 at 2819.923 kb on - strand, within Pf1N1B4_2486 at 2819.923 kb on - strand, within Pf1N1B4_2486 at 2819.923 kb on - strand, within Pf1N1B4_2486 at 2820.131 kb on + strand, within Pf1N1B4_2487 at 2820.132 kb on - strand, within Pf1N1B4_2487 at 2820.152 kb on + strand, within Pf1N1B4_2487 at 2820.152 kb on + strand, within Pf1N1B4_2487 at 2820.152 kb on + strand, within Pf1N1B4_2487 at 2820.153 kb on - strand, within Pf1N1B4_2487 at 2820.153 kb on - strand, within Pf1N1B4_2487 at 2820.153 kb on - strand, within Pf1N1B4_2487 at 2820.153 kb on - strand, within Pf1N1B4_2487 at 2820.290 kb on + strand, within Pf1N1B4_2487 at 2820.291 kb on - strand, within Pf1N1B4_2487 at 2820.401 kb on + strand, within Pf1N1B4_2487 at 2820.401 kb on + strand, within Pf1N1B4_2487 at 2820.402 kb on - strand at 2820.404 kb on + strand, within Pf1N1B4_2487 at 2820.404 kb on + strand, within Pf1N1B4_2487 at 2820.404 kb on + strand, within Pf1N1B4_2487 at 2820.404 kb on + strand, within Pf1N1B4_2487 at 2820.404 kb on + strand, within Pf1N1B4_2487 at 2820.404 kb on + strand, within Pf1N1B4_2487 at 2820.405 kb on - strand, within Pf1N1B4_2487 at 2820.405 kb on - strand, within Pf1N1B4_2487 at 2820.405 kb on - strand, within Pf1N1B4_2487 at 2820.405 kb on - strand, within Pf1N1B4_2487 at 2820.405 kb on - strand, within Pf1N1B4_2487 at 2820.437 kb on + strand, within Pf1N1B4_2487 at 2820.437 kb on + strand, within Pf1N1B4_2487 at 2820.437 kb on + strand, within Pf1N1B4_2487 at 2820.438 kb on - strand, within Pf1N1B4_2487 at 2820.460 kb on + strand, within Pf1N1B4_2487 at 2820.460 kb on + strand, within Pf1N1B4_2487 at 2820.460 kb on + strand, within Pf1N1B4_2487 at 2820.460 kb on + strand, within Pf1N1B4_2487 at 2820.464 kb on + strand, within Pf1N1B4_2487 at 2820.464 kb on + strand, within Pf1N1B4_2487 at 2820.491 kb on + strand, within Pf1N1B4_2487 at 2820.491 kb on + strand, within Pf1N1B4_2487 at 2820.491 kb on + strand, within Pf1N1B4_2487 at 2820.491 kb on + strand, within Pf1N1B4_2487 at 2820.491 kb on + strand, within Pf1N1B4_2487 at 2820.491 kb on + strand, within Pf1N1B4_2487 at 2820.491 kb on + strand, within Pf1N1B4_2487 at 2820.492 kb on - strand, within Pf1N1B4_2487 at 2820.977 kb on + strand at 2820.977 kb on + strand at 2820.977 kb on + strand at 2820.977 kb on + strand at 2820.977 kb on + strand at 2820.977 kb on + strand at 2820.977 kb on + strand at 2820.978 kb on - strand at 2820.978 kb on - strand at 2820.978 kb on - strand at 2820.978 kb on - strand at 2820.978 kb on - strand at 2821.002 kb on - strand at 2821.002 kb on - strand at 2821.002 kb on - strand at 2821.138 kb on - strand at 2821.138 kb on - strand at 2821.138 kb on - strand at 2821.138 kb on - strand at 2821.138 kb on - strand at 2821.233 kb on - strand at 2821.309 kb on + strand, within Pf1N1B4_2488 at 2821.309 kb on + strand, within Pf1N1B4_2488 at 2821.309 kb on + strand, within Pf1N1B4_2488 at 2821.319 kb on + strand, within Pf1N1B4_2488 at 2821.320 kb on - strand, within Pf1N1B4_2488 at 2821.499 kb on + strand, within Pf1N1B4_2488 at 2821.500 kb on - strand, within Pf1N1B4_2488 at 2821.500 kb on - strand, within Pf1N1B4_2488 at 2821.500 kb on - strand, within Pf1N1B4_2488 at 2821.500 kb on - strand, within Pf1N1B4_2488 at 2821.679 kb on - strand, within Pf1N1B4_2488 at 2821.964 kb on + strand, within Pf1N1B4_2488 at 2821.964 kb on + strand, within Pf1N1B4_2488 at 2821.964 kb on + strand, within Pf1N1B4_2488 at 2821.965 kb on - strand, within Pf1N1B4_2488 at 2821.965 kb on - strand, within Pf1N1B4_2488 at 2821.965 kb on - strand, within Pf1N1B4_2488 at 2821.965 kb on - strand, within Pf1N1B4_2488 at 2821.965 kb on - strand, within Pf1N1B4_2488 at 2821.965 kb on - strand, within Pf1N1B4_2488 at 2821.970 kb on + strand, within Pf1N1B4_2488 at 2821.970 kb on + strand, within Pf1N1B4_2488 at 2821.970 kb on + strand, within Pf1N1B4_2488 at 2821.970 kb on + strand, within Pf1N1B4_2488 at 2821.970 kb on + strand, within Pf1N1B4_2488 at 2821.971 kb on - strand, within Pf1N1B4_2488 at 2821.971 kb on - strand, within Pf1N1B4_2488 at 2821.971 kb on - strand, within Pf1N1B4_2488 at 2821.971 kb on - strand, within Pf1N1B4_2488 at 2821.971 kb on - strand, within Pf1N1B4_2488 at 2821.971 kb on - strand, within Pf1N1B4_2488 at 2821.971 kb on - strand, within Pf1N1B4_2488
Per-strain Table
Position Strand Gene LocusTag Fraction D-Gluconic Acid (C) remove 2,819,033 + Pf1N1B4_2484 0.42 -1.2 2,819,034 - Pf1N1B4_2484 0.43 -0.6 2,819,104 + Pf1N1B4_2484 0.77 +1.0 2,819,104 + Pf1N1B4_2484 0.77 -0.8 2,819,104 + +0.6 2,819,104 + Pf1N1B4_2484 0.77 +1.6 2,819,105 - Pf1N1B4_2484 0.77 +0.2 2,819,105 - Pf1N1B4_2484 0.77 +1.0 2,819,148 + -0.4 2,819,149 - +0.6 2,819,604 + Pf1N1B4_2485 0.78 -1.2 2,819,604 + Pf1N1B4_2485 0.78 -1.1 2,819,604 + Pf1N1B4_2485 0.78 -0.4 2,819,605 - Pf1N1B4_2485 0.78 -1.4 2,819,605 - Pf1N1B4_2485 0.78 -0.8 2,819,744 - -0.2 2,819,783 + -1.0 2,819,784 - -0.6 2,819,784 - -0.0 2,819,814 + -1.0 2,819,826 - -0.2 2,819,859 + -0.6 2,819,863 + -0.0 2,819,863 + +0.1 2,819,863 + +0.4 2,819,863 + +1.4 2,819,863 + -0.5 2,819,863 + -0.4 2,819,863 + +0.8 2,819,863 + +1.3 2,819,863 + +1.6 2,819,864 - -1.6 2,819,864 - -1.9 2,819,894 - Pf1N1B4_2486 0.32 +0.2 2,819,922 + Pf1N1B4_2486 0.56 -0.1 2,819,923 - Pf1N1B4_2486 0.57 -0.2 2,819,923 - Pf1N1B4_2486 0.57 -1.9 2,819,923 - Pf1N1B4_2486 0.57 -2.3 2,819,923 - Pf1N1B4_2486 0.57 -1.8 2,820,131 + Pf1N1B4_2487 0.18 -0.2 2,820,132 - Pf1N1B4_2487 0.18 -0.6 2,820,152 + Pf1N1B4_2487 0.20 -1.6 2,820,152 + Pf1N1B4_2487 0.20 -1.3 2,820,152 + Pf1N1B4_2487 0.20 -1.6 2,820,153 - Pf1N1B4_2487 0.20 +0.6 2,820,153 - Pf1N1B4_2487 0.20 -1.0 2,820,153 - Pf1N1B4_2487 0.20 -1.4 2,820,153 - Pf1N1B4_2487 0.20 -0.0 2,820,290 + Pf1N1B4_2487 0.33 -0.4 2,820,291 - Pf1N1B4_2487 0.33 -0.0 2,820,401 + Pf1N1B4_2487 0.44 +1.0 2,820,401 + Pf1N1B4_2487 0.44 +0.6 2,820,402 - -0.4 2,820,404 + Pf1N1B4_2487 0.44 -1.2 2,820,404 + Pf1N1B4_2487 0.44 +0.6 2,820,404 + Pf1N1B4_2487 0.44 -1.4 2,820,404 + Pf1N1B4_2487 0.44 -0.2 2,820,404 + Pf1N1B4_2487 0.44 +0.3 2,820,404 + Pf1N1B4_2487 0.44 -1.0 2,820,405 - Pf1N1B4_2487 0.44 -1.6 2,820,405 - Pf1N1B4_2487 0.44 -0.9 2,820,405 - Pf1N1B4_2487 0.44 -1.4 2,820,405 - Pf1N1B4_2487 0.44 -1.0 2,820,405 - Pf1N1B4_2487 0.44 -2.5 2,820,437 + Pf1N1B4_2487 0.47 -0.2 2,820,437 + Pf1N1B4_2487 0.47 -1.4 2,820,437 + Pf1N1B4_2487 0.47 +0.6 2,820,438 - Pf1N1B4_2487 0.47 -0.0 2,820,460 + Pf1N1B4_2487 0.49 -1.0 2,820,460 + Pf1N1B4_2487 0.49 -2.1 2,820,460 + Pf1N1B4_2487 0.49 -0.8 2,820,460 + Pf1N1B4_2487 0.49 +1.1 2,820,464 + Pf1N1B4_2487 0.50 -1.2 2,820,464 + Pf1N1B4_2487 0.50 +0.3 2,820,491 + Pf1N1B4_2487 0.52 -2.4 2,820,491 + Pf1N1B4_2487 0.52 -0.7 2,820,491 + Pf1N1B4_2487 0.52 -2.4 2,820,491 + Pf1N1B4_2487 0.52 +0.2 2,820,491 + Pf1N1B4_2487 0.52 -1.7 2,820,491 + Pf1N1B4_2487 0.52 -1.2 2,820,491 + Pf1N1B4_2487 0.52 -1.3 2,820,492 - Pf1N1B4_2487 0.52 -1.4 2,820,977 + -1.2 2,820,977 + +0.7 2,820,977 + +0.6 2,820,977 + -0.4 2,820,977 + +1.0 2,820,977 + -1.2 2,820,977 + -0.0 2,820,978 - -1.2 2,820,978 - -1.2 2,820,978 - -1.0 2,820,978 - -0.8 2,820,978 - -0.4 2,821,002 - -1.0 2,821,002 - -0.4 2,821,002 - -1.3 2,821,138 - -1.1 2,821,138 - -1.6 2,821,138 - -2.6 2,821,138 - -1.8 2,821,138 - -0.0 2,821,233 - -1.1 2,821,309 + Pf1N1B4_2488 0.11 -3.0 2,821,309 + Pf1N1B4_2488 0.11 -0.0 2,821,309 + Pf1N1B4_2488 0.11 -1.0 2,821,319 + Pf1N1B4_2488 0.12 -0.8 2,821,320 - Pf1N1B4_2488 0.12 -1.1 2,821,499 + Pf1N1B4_2488 0.27 -1.0 2,821,500 - Pf1N1B4_2488 0.27 -1.2 2,821,500 - Pf1N1B4_2488 0.27 +0.6 2,821,500 - Pf1N1B4_2488 0.27 -2.2 2,821,500 - Pf1N1B4_2488 0.27 -1.4 2,821,679 - Pf1N1B4_2488 0.42 -1.0 2,821,964 + Pf1N1B4_2488 0.66 -1.0 2,821,964 + Pf1N1B4_2488 0.66 -0.0 2,821,964 + Pf1N1B4_2488 0.66 -1.2 2,821,965 - Pf1N1B4_2488 0.66 -1.2 2,821,965 - Pf1N1B4_2488 0.66 -1.0 2,821,965 - Pf1N1B4_2488 0.66 -2.1 2,821,965 - Pf1N1B4_2488 0.66 -0.2 2,821,965 - Pf1N1B4_2488 0.66 -2.3 2,821,965 - Pf1N1B4_2488 0.66 -2.1 2,821,970 + Pf1N1B4_2488 0.67 -0.1 2,821,970 + Pf1N1B4_2488 0.67 -1.0 2,821,970 + Pf1N1B4_2488 0.67 -0.8 2,821,970 + Pf1N1B4_2488 0.67 -0.8 2,821,970 + Pf1N1B4_2488 0.67 -1.0 2,821,971 - Pf1N1B4_2488 0.67 -0.9 2,821,971 - Pf1N1B4_2488 0.67 -2.0 2,821,971 - Pf1N1B4_2488 0.67 -0.4 2,821,971 - Pf1N1B4_2488 0.67 -0.0 2,821,971 - Pf1N1B4_2488 0.67 -1.8 2,821,971 - Pf1N1B4_2488 0.67 -0.8 2,821,971 - Pf1N1B4_2488 0.67 -0.9
Or see this region's nucleotide sequence