Experiment: D-Gluconic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_237 and Pf1N1B4_238 are separated by 76 nucleotides Pf1N1B4_238 and Pf1N1B4_239 are separated by 89 nucleotides Pf1N1B4_239 and Pf1N1B4_240 are separated by 73 nucleotides Pf1N1B4_240 and Pf1N1B4_241 are separated by 163 nucleotides
Pf1N1B4_237 - Molybdopterin-guanine dinucleotide biosynthesis protein MobA, at 297,049 to 297,651
_237
Pf1N1B4_238 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 297,728 to 297,949
_238
Pf1N1B4_239 - Similar to ribosomal large subunit pseudouridine synthase A, at 298,039 to 298,929
_239
Pf1N1B4_240 - Transcriptional regulators, at 299,003 to 299,323
_240
Pf1N1B4_241 - Thiol-disulfide isomerase and thioredoxins, at 299,487 to 299,744
_241
Position (kb) 298
299 Strain fitness (log2 ratio) -2
-1
0
1
2 at 297.045 kb on + strand at 297.045 kb on + strand at 297.045 kb on + strand at 297.045 kb on + strand at 297.045 kb on + strand at 297.046 kb on - strand at 297.046 kb on - strand at 297.060 kb on + strand at 297.060 kb on + strand at 297.061 kb on - strand at 297.061 kb on - strand at 297.061 kb on - strand at 297.238 kb on + strand, within Pf1N1B4_237 at 297.239 kb on - strand, within Pf1N1B4_237 at 297.239 kb on - strand, within Pf1N1B4_237 at 297.239 kb on - strand, within Pf1N1B4_237 at 297.341 kb on + strand, within Pf1N1B4_237 at 297.341 kb on + strand, within Pf1N1B4_237 at 297.341 kb on + strand, within Pf1N1B4_237 at 297.342 kb on - strand, within Pf1N1B4_237 at 297.543 kb on + strand, within Pf1N1B4_237 at 297.543 kb on + strand, within Pf1N1B4_237 at 297.609 kb on + strand at 297.623 kb on + strand at 297.677 kb on + strand at 297.677 kb on + strand at 297.678 kb on - strand at 297.678 kb on - strand at 297.679 kb on + strand at 297.679 kb on + strand at 297.679 kb on + strand at 297.679 kb on + strand at 297.679 kb on + strand at 297.679 kb on + strand at 297.679 kb on + strand at 297.679 kb on + strand at 297.680 kb on - strand at 297.800 kb on - strand at 297.947 kb on + strand at 297.970 kb on + strand at 297.970 kb on + strand at 297.988 kb on + strand at 298.011 kb on - strand at 298.011 kb on - strand at 298.140 kb on - strand, within Pf1N1B4_239 at 298.140 kb on - strand, within Pf1N1B4_239 at 298.140 kb on - strand, within Pf1N1B4_239 at 298.151 kb on - strand, within Pf1N1B4_239 at 298.251 kb on - strand, within Pf1N1B4_239 at 298.360 kb on - strand, within Pf1N1B4_239 at 298.372 kb on - strand, within Pf1N1B4_239 at 298.713 kb on - strand, within Pf1N1B4_239 at 298.713 kb on - strand, within Pf1N1B4_239 at 298.713 kb on - strand, within Pf1N1B4_239 at 298.736 kb on + strand, within Pf1N1B4_239 at 298.737 kb on - strand at 298.880 kb on + strand at 298.880 kb on + strand at 298.881 kb on - strand at 298.881 kb on - strand at 298.953 kb on - strand at 298.990 kb on + strand at 298.990 kb on + strand at 298.991 kb on - strand at 298.991 kb on - strand at 299.103 kb on + strand, within Pf1N1B4_240 at 299.103 kb on + strand, within Pf1N1B4_240 at 299.103 kb on + strand, within Pf1N1B4_240 at 299.189 kb on - strand, within Pf1N1B4_240 at 299.357 kb on + strand at 299.411 kb on - strand at 299.576 kb on + strand, within Pf1N1B4_241 at 299.587 kb on + strand, within Pf1N1B4_241 at 299.750 kb on + strand at 299.750 kb on + strand at 299.750 kb on + strand at 299.751 kb on - strand at 299.751 kb on - strand at 299.763 kb on + strand at 299.764 kb on - strand at 299.798 kb on + strand at 299.900 kb on + strand at 299.900 kb on + strand at 299.900 kb on + strand at 299.900 kb on + strand at 299.900 kb on + strand at 299.901 kb on - strand at 299.901 kb on - strand at 299.901 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Gluconic Acid (C) remove 297,045 + -1.1 297,045 + -1.0 297,045 + +0.6 297,045 + -0.3 297,045 + +1.5 297,046 - +1.0 297,046 - -0.2 297,060 + +0.0 297,060 + +0.0 297,061 - +0.2 297,061 - +0.3 297,061 - -0.4 297,238 + Pf1N1B4_237 0.31 +2.4 297,239 - Pf1N1B4_237 0.32 -0.4 297,239 - Pf1N1B4_237 0.32 -1.3 297,239 - Pf1N1B4_237 0.32 +0.2 297,341 + Pf1N1B4_237 0.48 +0.0 297,341 + Pf1N1B4_237 0.48 +0.6 297,341 + Pf1N1B4_237 0.48 -2.3 297,342 - Pf1N1B4_237 0.49 -0.4 297,543 + Pf1N1B4_237 0.82 +0.3 297,543 + Pf1N1B4_237 0.82 -1.4 297,609 + -0.4 297,623 + -0.3 297,677 + +1.0 297,677 + +0.5 297,678 - +1.3 297,678 - -1.3 297,679 + +0.7 297,679 + +1.5 297,679 + -1.1 297,679 + +0.2 297,679 + -1.9 297,679 + +2.6 297,679 + +1.0 297,679 + +0.7 297,680 - +1.0 297,800 - +0.6 297,947 + -0.5 297,970 + +1.9 297,970 + -0.8 297,988 + +0.0 298,011 - -0.3 298,011 - -0.0 298,140 - Pf1N1B4_239 0.11 -1.4 298,140 - Pf1N1B4_239 0.11 -0.5 298,140 - Pf1N1B4_239 0.11 +0.2 298,151 - Pf1N1B4_239 0.13 -1.0 298,251 - Pf1N1B4_239 0.24 -0.7 298,360 - Pf1N1B4_239 0.36 -0.2 298,372 - Pf1N1B4_239 0.37 +1.0 298,713 - Pf1N1B4_239 0.76 -2.0 298,713 - Pf1N1B4_239 0.76 +0.0 298,713 - Pf1N1B4_239 0.76 -1.8 298,736 + Pf1N1B4_239 0.78 -1.3 298,737 - +0.0 298,880 + +1.1 298,880 + +0.5 298,881 - -0.7 298,881 - +0.8 298,953 - -0.5 298,990 + +0.1 298,990 + +0.7 298,991 - +0.1 298,991 - -0.9 299,103 + Pf1N1B4_240 0.31 -1.8 299,103 + Pf1N1B4_240 0.31 -1.5 299,103 + Pf1N1B4_240 0.31 +0.4 299,189 - Pf1N1B4_240 0.58 -1.3 299,357 + +1.9 299,411 - +1.1 299,576 + Pf1N1B4_241 0.34 -0.6 299,587 + Pf1N1B4_241 0.39 -1.5 299,750 + +0.9 299,750 + +1.4 299,750 + -0.4 299,751 - +2.6 299,751 - +0.7 299,763 + -0.2 299,764 - +1.3 299,798 + +0.5 299,900 + -0.0 299,900 + -0.1 299,900 + +0.6 299,900 + -0.8 299,900 + -0.1 299,901 - +0.1 299,901 - -0.2 299,901 - -0.3
Or see this region's nucleotide sequence