Experiment: D-Gluconic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2278 and Pf1N1B4_2279 are separated by 108 nucleotides Pf1N1B4_2279 and Pf1N1B4_2280 are separated by 21 nucleotides Pf1N1B4_2280 and Pf1N1B4_2281 are separated by 39 nucleotides Pf1N1B4_2281 and Pf1N1B4_2282 are separated by 54 nucleotides
Pf1N1B4_2278 - Ribose 5-phosphate isomerase A (EC 5.3.1.6), at 2,594,127 to 2,594,801
_2278
Pf1N1B4_2279 - hypothetical protein, at 2,594,910 to 2,595,056
_2279
Pf1N1B4_2280 - Threonine dehydratase biosynthetic (EC 4.3.1.19), at 2,595,078 to 2,596,592
_2280
Pf1N1B4_2281 - Predicted integral membrane protein, at 2,596,632 to 2,597,123
_2281
Pf1N1B4_2282 - Phosphoserine phosphatase (EC 3.1.3.3), at 2,597,178 to 2,597,834
_2282
Position (kb)
2595
2596
2597 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2594.093 kb on + strand at 2594.093 kb on + strand at 2594.094 kb on - strand at 2594.109 kb on - strand at 2594.844 kb on - strand at 2594.845 kb on + strand at 2594.845 kb on + strand at 2594.845 kb on + strand at 2594.845 kb on + strand at 2594.845 kb on + strand at 2594.845 kb on + strand at 2594.846 kb on - strand at 2594.846 kb on - strand at 2594.846 kb on - strand at 2594.846 kb on - strand at 2594.846 kb on - strand at 2594.911 kb on + strand at 2594.911 kb on + strand at 2594.917 kb on + strand at 2594.918 kb on - strand at 2594.918 kb on - strand at 2594.959 kb on + strand at 2594.959 kb on + strand, within Pf1N1B4_2279 at 2594.959 kb on + strand, within Pf1N1B4_2279 at 2594.959 kb on + strand, within Pf1N1B4_2279 at 2594.960 kb on - strand, within Pf1N1B4_2279 at 2594.960 kb on - strand, within Pf1N1B4_2279 at 2594.960 kb on - strand, within Pf1N1B4_2279 at 2594.960 kb on - strand, within Pf1N1B4_2279 at 2594.960 kb on - strand, within Pf1N1B4_2279 at 2594.960 kb on - strand, within Pf1N1B4_2279 at 2594.960 kb on - strand, within Pf1N1B4_2279 at 2594.960 kb on - strand, within Pf1N1B4_2279 at 2594.963 kb on + strand, within Pf1N1B4_2279 at 2594.963 kb on + strand, within Pf1N1B4_2279 at 2594.963 kb on + strand, within Pf1N1B4_2279 at 2594.963 kb on + strand, within Pf1N1B4_2279 at 2594.963 kb on + strand, within Pf1N1B4_2279 at 2594.964 kb on - strand, within Pf1N1B4_2279 at 2594.964 kb on - strand, within Pf1N1B4_2279 at 2594.964 kb on - strand, within Pf1N1B4_2279 at 2594.964 kb on - strand, within Pf1N1B4_2279 at 2594.973 kb on - strand, within Pf1N1B4_2279 at 2594.973 kb on - strand, within Pf1N1B4_2279 at 2595.003 kb on + strand, within Pf1N1B4_2279 at 2595.004 kb on - strand, within Pf1N1B4_2279 at 2595.004 kb on - strand, within Pf1N1B4_2279 at 2595.090 kb on + strand at 2595.090 kb on + strand at 2595.090 kb on + strand at 2595.090 kb on + strand at 2595.090 kb on + strand at 2595.091 kb on - strand at 2595.091 kb on - strand at 2595.091 kb on - strand at 2595.091 kb on - strand at 2595.091 kb on - strand at 2595.091 kb on - strand at 2595.091 kb on - strand at 2595.121 kb on - strand at 2595.121 kb on - strand at 2595.121 kb on - strand at 2595.133 kb on + strand at 2595.186 kb on - strand at 2595.186 kb on - strand at 2595.243 kb on + strand at 2595.243 kb on + strand, within Pf1N1B4_2280 at 2595.244 kb on - strand, within Pf1N1B4_2280 at 2595.244 kb on - strand, within Pf1N1B4_2280 at 2595.245 kb on + strand, within Pf1N1B4_2280 at 2595.496 kb on - strand, within Pf1N1B4_2280 at 2595.501 kb on + strand, within Pf1N1B4_2280 at 2595.501 kb on + strand, within Pf1N1B4_2280 at 2595.502 kb on - strand, within Pf1N1B4_2280 at 2595.502 kb on - strand, within Pf1N1B4_2280 at 2595.502 kb on - strand, within Pf1N1B4_2280 at 2595.502 kb on - strand, within Pf1N1B4_2280 at 2595.513 kb on + strand, within Pf1N1B4_2280 at 2595.513 kb on + strand, within Pf1N1B4_2280 at 2595.651 kb on + strand, within Pf1N1B4_2280 at 2595.651 kb on + strand, within Pf1N1B4_2280 at 2595.651 kb on + strand, within Pf1N1B4_2280 at 2595.651 kb on + strand, within Pf1N1B4_2280 at 2595.652 kb on - strand, within Pf1N1B4_2280 at 2595.652 kb on - strand, within Pf1N1B4_2280 at 2595.819 kb on + strand, within Pf1N1B4_2280 at 2595.819 kb on + strand, within Pf1N1B4_2280 at 2595.819 kb on + strand, within Pf1N1B4_2280 at 2595.820 kb on - strand, within Pf1N1B4_2280 at 2595.820 kb on - strand, within Pf1N1B4_2280 at 2595.824 kb on - strand, within Pf1N1B4_2280 at 2595.876 kb on - strand, within Pf1N1B4_2280 at 2595.879 kb on + strand, within Pf1N1B4_2280 at 2595.880 kb on - strand, within Pf1N1B4_2280 at 2595.942 kb on + strand, within Pf1N1B4_2280 at 2595.942 kb on + strand, within Pf1N1B4_2280 at 2595.942 kb on + strand, within Pf1N1B4_2280 at 2595.942 kb on + strand, within Pf1N1B4_2280 at 2595.942 kb on + strand, within Pf1N1B4_2280 at 2595.942 kb on + strand, within Pf1N1B4_2280 at 2595.942 kb on + strand, within Pf1N1B4_2280 at 2595.942 kb on + strand, within Pf1N1B4_2280 at 2595.942 kb on + strand, within Pf1N1B4_2280 at 2595.942 kb on + strand, within Pf1N1B4_2280 at 2595.943 kb on - strand, within Pf1N1B4_2280 at 2595.943 kb on - strand, within Pf1N1B4_2280 at 2595.943 kb on - strand, within Pf1N1B4_2280 at 2595.943 kb on - strand at 2595.943 kb on - strand, within Pf1N1B4_2280 at 2596.152 kb on + strand, within Pf1N1B4_2280 at 2596.152 kb on + strand, within Pf1N1B4_2280 at 2596.159 kb on - strand, within Pf1N1B4_2280 at 2596.310 kb on + strand, within Pf1N1B4_2280 at 2596.310 kb on + strand, within Pf1N1B4_2280 at 2596.471 kb on + strand at 2596.471 kb on + strand at 2596.546 kb on - strand at 2596.546 kb on - strand at 2596.573 kb on - strand at 2596.573 kb on - strand at 2596.665 kb on - strand at 2596.665 kb on - strand at 2596.665 kb on - strand at 2596.760 kb on + strand, within Pf1N1B4_2281 at 2596.761 kb on - strand, within Pf1N1B4_2281 at 2596.761 kb on - strand, within Pf1N1B4_2281 at 2596.761 kb on - strand, within Pf1N1B4_2281 at 2596.761 kb on - strand, within Pf1N1B4_2281 at 2596.900 kb on - strand, within Pf1N1B4_2281 at 2596.900 kb on - strand, within Pf1N1B4_2281 at 2596.982 kb on + strand, within Pf1N1B4_2281 at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.121 kb on + strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.122 kb on - strand at 2597.125 kb on + strand at 2597.125 kb on + strand at 2597.126 kb on - strand at 2597.126 kb on - strand at 2597.126 kb on - strand at 2597.126 kb on - strand at 2597.126 kb on - strand at 2597.126 kb on - strand at 2597.126 kb on - strand at 2597.126 kb on - strand at 2597.305 kb on + strand, within Pf1N1B4_2282 at 2597.305 kb on + strand, within Pf1N1B4_2282 at 2597.305 kb on + strand, within Pf1N1B4_2282 at 2597.306 kb on - strand, within Pf1N1B4_2282 at 2597.306 kb on - strand, within Pf1N1B4_2282 at 2597.306 kb on - strand, within Pf1N1B4_2282 at 2597.306 kb on - strand, within Pf1N1B4_2282 at 2597.311 kb on + strand, within Pf1N1B4_2282 at 2597.311 kb on + strand, within Pf1N1B4_2282 at 2597.311 kb on + strand, within Pf1N1B4_2282 at 2597.311 kb on + strand, within Pf1N1B4_2282 at 2597.311 kb on + strand, within Pf1N1B4_2282 at 2597.311 kb on + strand, within Pf1N1B4_2282 at 2597.311 kb on + strand, within Pf1N1B4_2282 at 2597.311 kb on + strand, within Pf1N1B4_2282 at 2597.312 kb on - strand, within Pf1N1B4_2282 at 2597.312 kb on - strand, within Pf1N1B4_2282 at 2597.312 kb on - strand, within Pf1N1B4_2282 at 2597.312 kb on - strand, within Pf1N1B4_2282 at 2597.312 kb on - strand, within Pf1N1B4_2282 at 2597.312 kb on - strand, within Pf1N1B4_2282 at 2597.312 kb on - strand, within Pf1N1B4_2282 at 2597.312 kb on - strand, within Pf1N1B4_2282 at 2597.312 kb on - strand, within Pf1N1B4_2282
Per-strain Table
Position Strand Gene LocusTag Fraction D-Gluconic Acid (C) remove 2,594,093 + -0.2 2,594,093 + +0.6 2,594,094 - -0.6 2,594,109 - +0.5 2,594,844 - -0.7 2,594,845 + +0.6 2,594,845 + -0.2 2,594,845 + +0.4 2,594,845 + -0.4 2,594,845 + -0.7 2,594,845 + -1.3 2,594,846 - -1.7 2,594,846 - -1.5 2,594,846 - +0.3 2,594,846 - -0.4 2,594,846 - -0.2 2,594,911 + -0.1 2,594,911 + -0.7 2,594,917 + +0.6 2,594,918 - -0.5 2,594,918 - +0.4 2,594,959 + +0.6 2,594,959 + Pf1N1B4_2279 0.33 +0.1 2,594,959 + Pf1N1B4_2279 0.33 +2.9 2,594,959 + Pf1N1B4_2279 0.33 -1.8 2,594,960 - Pf1N1B4_2279 0.34 -0.5 2,594,960 - Pf1N1B4_2279 0.34 -0.4 2,594,960 - Pf1N1B4_2279 0.34 -0.4 2,594,960 - Pf1N1B4_2279 0.34 +0.6 2,594,960 - Pf1N1B4_2279 0.34 -1.0 2,594,960 - Pf1N1B4_2279 0.34 +0.9 2,594,960 - Pf1N1B4_2279 0.34 +0.2 2,594,960 - Pf1N1B4_2279 0.34 +0.8 2,594,963 + Pf1N1B4_2279 0.36 -1.0 2,594,963 + Pf1N1B4_2279 0.36 +0.6 2,594,963 + Pf1N1B4_2279 0.36 +0.5 2,594,963 + Pf1N1B4_2279 0.36 -1.0 2,594,963 + Pf1N1B4_2279 0.36 -0.5 2,594,964 - Pf1N1B4_2279 0.37 +0.6 2,594,964 - Pf1N1B4_2279 0.37 -0.6 2,594,964 - Pf1N1B4_2279 0.37 +0.6 2,594,964 - Pf1N1B4_2279 0.37 +0.9 2,594,973 - Pf1N1B4_2279 0.43 +0.3 2,594,973 - Pf1N1B4_2279 0.43 -1.4 2,595,003 + Pf1N1B4_2279 0.63 -0.4 2,595,004 - Pf1N1B4_2279 0.64 +1.3 2,595,004 - Pf1N1B4_2279 0.64 +0.0 2,595,090 + -1.5 2,595,090 + -2.0 2,595,090 + -2.5 2,595,090 + -1.2 2,595,090 + -1.2 2,595,091 - -0.4 2,595,091 - -1.5 2,595,091 - -0.9 2,595,091 - -1.5 2,595,091 - -1.7 2,595,091 - -1.5 2,595,091 - -0.7 2,595,121 - +1.0 2,595,121 - -0.7 2,595,121 - -2.5 2,595,133 + -1.3 2,595,186 - -1.5 2,595,186 - -1.8 2,595,243 + -1.0 2,595,243 + Pf1N1B4_2280 0.11 -1.6 2,595,244 - Pf1N1B4_2280 0.11 -2.1 2,595,244 - Pf1N1B4_2280 0.11 -1.7 2,595,245 + Pf1N1B4_2280 0.11 -1.2 2,595,496 - Pf1N1B4_2280 0.28 -0.7 2,595,501 + Pf1N1B4_2280 0.28 -0.7 2,595,501 + Pf1N1B4_2280 0.28 -0.2 2,595,502 - Pf1N1B4_2280 0.28 -1.8 2,595,502 - Pf1N1B4_2280 0.28 -1.7 2,595,502 - Pf1N1B4_2280 0.28 +0.6 2,595,502 - Pf1N1B4_2280 0.28 -0.8 2,595,513 + Pf1N1B4_2280 0.29 -1.0 2,595,513 + Pf1N1B4_2280 0.29 -3.0 2,595,651 + Pf1N1B4_2280 0.38 -1.8 2,595,651 + Pf1N1B4_2280 0.38 -0.8 2,595,651 + Pf1N1B4_2280 0.38 -1.0 2,595,651 + Pf1N1B4_2280 0.38 -1.7 2,595,652 - Pf1N1B4_2280 0.38 -0.7 2,595,652 - Pf1N1B4_2280 0.38 -1.7 2,595,819 + Pf1N1B4_2280 0.49 +0.6 2,595,819 + Pf1N1B4_2280 0.49 -0.2 2,595,819 + Pf1N1B4_2280 0.49 -2.4 2,595,820 - Pf1N1B4_2280 0.49 -2.9 2,595,820 - Pf1N1B4_2280 0.49 -1.4 2,595,824 - Pf1N1B4_2280 0.49 +0.0 2,595,876 - Pf1N1B4_2280 0.53 -2.3 2,595,879 + Pf1N1B4_2280 0.53 -0.9 2,595,880 - Pf1N1B4_2280 0.53 -0.7 2,595,942 + Pf1N1B4_2280 0.57 -0.4 2,595,942 + Pf1N1B4_2280 0.57 -0.7 2,595,942 + Pf1N1B4_2280 0.57 +0.6 2,595,942 + Pf1N1B4_2280 0.57 +0.6 2,595,942 + Pf1N1B4_2280 0.57 -0.7 2,595,942 + Pf1N1B4_2280 0.57 -2.1 2,595,942 + Pf1N1B4_2280 0.57 +0.0 2,595,942 + Pf1N1B4_2280 0.57 -2.1 2,595,942 + Pf1N1B4_2280 0.57 +0.2 2,595,942 + Pf1N1B4_2280 0.57 -1.0 2,595,943 - Pf1N1B4_2280 0.57 -1.0 2,595,943 - Pf1N1B4_2280 0.57 +0.6 2,595,943 - Pf1N1B4_2280 0.57 -1.5 2,595,943 - -0.4 2,595,943 - Pf1N1B4_2280 0.57 -0.4 2,596,152 + Pf1N1B4_2280 0.71 -1.0 2,596,152 + Pf1N1B4_2280 0.71 -1.0 2,596,159 - Pf1N1B4_2280 0.71 -1.4 2,596,310 + Pf1N1B4_2280 0.81 -0.3 2,596,310 + Pf1N1B4_2280 0.81 +0.6 2,596,471 + +0.1 2,596,471 + -1.8 2,596,546 - +0.3 2,596,546 - -0.4 2,596,573 - -0.9 2,596,573 - -1.4 2,596,665 - +0.1 2,596,665 - +0.1 2,596,665 - -0.9 2,596,760 + Pf1N1B4_2281 0.26 +0.2 2,596,761 - Pf1N1B4_2281 0.26 +0.1 2,596,761 - Pf1N1B4_2281 0.26 +0.2 2,596,761 - Pf1N1B4_2281 0.26 +2.6 2,596,761 - Pf1N1B4_2281 0.26 -0.5 2,596,900 - Pf1N1B4_2281 0.54 -0.6 2,596,900 - Pf1N1B4_2281 0.54 -0.4 2,596,982 + Pf1N1B4_2281 0.71 +0.9 2,597,121 + -0.9 2,597,121 + +0.2 2,597,121 + -0.9 2,597,121 + -0.9 2,597,121 + -0.4 2,597,121 + -0.1 2,597,121 + -0.7 2,597,121 + -1.1 2,597,121 + +0.1 2,597,121 + -0.2 2,597,121 + +0.3 2,597,121 + -1.2 2,597,121 + -1.3 2,597,121 + +0.9 2,597,121 + +1.1 2,597,121 + +1.1 2,597,121 + -0.9 2,597,121 + -1.1 2,597,121 + +1.3 2,597,121 + -0.0 2,597,121 + -0.7 2,597,121 + -1.4 2,597,121 + +0.1 2,597,121 + -0.7 2,597,121 + -0.7 2,597,121 + +1.3 2,597,121 + +1.1 2,597,122 - +0.4 2,597,122 - +3.1 2,597,122 - +0.6 2,597,122 - +0.1 2,597,122 - +0.4 2,597,122 - -1.7 2,597,122 - -0.2 2,597,122 - +0.2 2,597,122 - -1.4 2,597,122 - -0.4 2,597,122 - +0.1 2,597,122 - -2.3 2,597,122 - +0.1 2,597,122 - -0.4 2,597,122 - -0.9 2,597,122 - -0.4 2,597,122 - +1.0 2,597,122 - -1.5 2,597,122 - -1.2 2,597,122 - -0.1 2,597,122 - -0.5 2,597,122 - -0.9 2,597,122 - +0.1 2,597,122 - -0.2 2,597,122 - -0.5 2,597,122 - -1.4 2,597,125 + +0.8 2,597,125 + -0.1 2,597,126 - -0.4 2,597,126 - -0.5 2,597,126 - +0.8 2,597,126 - +0.4 2,597,126 - +0.3 2,597,126 - +0.9 2,597,126 - -1.2 2,597,126 - +0.6 2,597,305 + Pf1N1B4_2282 0.19 -0.4 2,597,305 + Pf1N1B4_2282 0.19 -1.7 2,597,305 + Pf1N1B4_2282 0.19 -2.4 2,597,306 - Pf1N1B4_2282 0.19 -0.7 2,597,306 - Pf1N1B4_2282 0.19 -2.1 2,597,306 - Pf1N1B4_2282 0.19 +0.3 2,597,306 - Pf1N1B4_2282 0.19 +0.6 2,597,311 + Pf1N1B4_2282 0.20 +0.1 2,597,311 + Pf1N1B4_2282 0.20 -0.7 2,597,311 + Pf1N1B4_2282 0.20 -2.4 2,597,311 + Pf1N1B4_2282 0.20 -1.5 2,597,311 + Pf1N1B4_2282 0.20 -3.0 2,597,311 + Pf1N1B4_2282 0.20 -1.4 2,597,311 + Pf1N1B4_2282 0.20 -2.2 2,597,311 + Pf1N1B4_2282 0.20 -2.4 2,597,312 - Pf1N1B4_2282 0.20 -2.5 2,597,312 - Pf1N1B4_2282 0.20 -1.2 2,597,312 - Pf1N1B4_2282 0.20 -1.5 2,597,312 - Pf1N1B4_2282 0.20 -1.5 2,597,312 - Pf1N1B4_2282 0.20 -0.5 2,597,312 - Pf1N1B4_2282 0.20 -1.4 2,597,312 - Pf1N1B4_2282 0.20 +0.2 2,597,312 - Pf1N1B4_2282 0.20 -0.4 2,597,312 - Pf1N1B4_2282 0.20 -1.5
Or see this region's nucleotide sequence