Experiment: D-Gluconic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2208 and Pf1N1B4_2209 are separated by 174 nucleotides Pf1N1B4_2209 and Pf1N1B4_2210 are separated by 208 nucleotides Pf1N1B4_2210 and Pf1N1B4_2211 overlap by 14 nucleotides
Pf1N1B4_2208 - Polyphosphate kinase (EC 2.7.4.1), at 2,520,482 to 2,522,704
_2208
Pf1N1B4_2209 - Exopolyphosphatase (EC 3.6.1.11), at 2,522,879 to 2,524,381
_2209
Pf1N1B4_2210 - Glutamate transport ATP-binding protein, at 2,524,590 to 2,525,327
_2210
Pf1N1B4_2211 - Putative amino acid ABC transporter, permease protein, at 2,525,314 to 2,525,964
_2211
Position (kb)
2522
2523
2524
2525 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2521.910 kb on + strand, within Pf1N1B4_2208 at 2522.010 kb on - strand, within Pf1N1B4_2208 at 2522.042 kb on + strand, within Pf1N1B4_2208 at 2522.043 kb on - strand, within Pf1N1B4_2208 at 2522.279 kb on + strand, within Pf1N1B4_2208 at 2522.280 kb on - strand, within Pf1N1B4_2208 at 2522.280 kb on - strand, within Pf1N1B4_2208 at 2522.420 kb on + strand, within Pf1N1B4_2208 at 2522.421 kb on - strand, within Pf1N1B4_2208 at 2522.561 kb on + strand at 2522.660 kb on - strand at 2522.707 kb on + strand at 2522.707 kb on + strand at 2522.707 kb on + strand at 2522.707 kb on + strand at 2522.707 kb on + strand at 2522.708 kb on - strand at 2523.011 kb on + strand at 2523.011 kb on + strand at 2523.012 kb on - strand at 2523.012 kb on - strand at 2523.012 kb on - strand at 2523.012 kb on - strand at 2523.012 kb on - strand at 2523.225 kb on + strand, within Pf1N1B4_2209 at 2523.226 kb on - strand, within Pf1N1B4_2209 at 2523.226 kb on - strand, within Pf1N1B4_2209 at 2523.243 kb on + strand, within Pf1N1B4_2209 at 2523.244 kb on - strand, within Pf1N1B4_2209 at 2523.244 kb on - strand, within Pf1N1B4_2209 at 2523.249 kb on + strand, within Pf1N1B4_2209 at 2523.249 kb on + strand, within Pf1N1B4_2209 at 2523.250 kb on - strand, within Pf1N1B4_2209 at 2523.250 kb on - strand, within Pf1N1B4_2209 at 2523.250 kb on - strand, within Pf1N1B4_2209 at 2523.474 kb on + strand, within Pf1N1B4_2209 at 2523.474 kb on + strand, within Pf1N1B4_2209 at 2523.475 kb on - strand, within Pf1N1B4_2209 at 2523.475 kb on - strand, within Pf1N1B4_2209 at 2523.810 kb on + strand, within Pf1N1B4_2209 at 2523.810 kb on + strand, within Pf1N1B4_2209 at 2523.810 kb on + strand, within Pf1N1B4_2209 at 2523.811 kb on - strand, within Pf1N1B4_2209 at 2523.855 kb on + strand, within Pf1N1B4_2209 at 2523.856 kb on - strand, within Pf1N1B4_2209 at 2523.856 kb on - strand, within Pf1N1B4_2209 at 2523.867 kb on + strand, within Pf1N1B4_2209 at 2523.867 kb on + strand, within Pf1N1B4_2209 at 2523.867 kb on + strand, within Pf1N1B4_2209 at 2523.867 kb on + strand, within Pf1N1B4_2209 at 2523.867 kb on + strand, within Pf1N1B4_2209 at 2523.867 kb on + strand, within Pf1N1B4_2209 at 2523.867 kb on + strand, within Pf1N1B4_2209 at 2523.868 kb on - strand, within Pf1N1B4_2209 at 2524.008 kb on + strand, within Pf1N1B4_2209 at 2524.009 kb on - strand, within Pf1N1B4_2209 at 2524.009 kb on - strand, within Pf1N1B4_2209 at 2524.151 kb on - strand, within Pf1N1B4_2209 at 2524.512 kb on + strand at 2524.548 kb on + strand at 2524.548 kb on + strand at 2524.549 kb on - strand at 2524.656 kb on + strand at 2524.656 kb on + strand at 2524.656 kb on + strand at 2524.657 kb on - strand at 2524.657 kb on - strand at 2524.657 kb on - strand at 2524.657 kb on - strand at 2525.131 kb on + strand, within Pf1N1B4_2210 at 2525.132 kb on - strand, within Pf1N1B4_2210 at 2525.132 kb on - strand at 2525.293 kb on + strand at 2525.293 kb on + strand at 2525.293 kb on + strand at 2525.293 kb on + strand at 2525.293 kb on + strand at 2525.293 kb on + strand at 2525.294 kb on - strand at 2525.294 kb on - strand at 2525.294 kb on - strand at 2525.294 kb on - strand at 2525.294 kb on - strand at 2525.294 kb on - strand at 2525.294 kb on - strand at 2525.297 kb on + strand at 2525.297 kb on + strand at 2525.297 kb on + strand at 2525.298 kb on - strand at 2525.298 kb on - strand at 2525.298 kb on - strand at 2525.298 kb on - strand at 2525.298 kb on - strand at 2525.298 kb on - strand at 2525.298 kb on - strand at 2525.298 kb on - strand at 2525.298 kb on - strand at 2525.298 kb on - strand at 2525.315 kb on + strand at 2525.315 kb on + strand at 2525.315 kb on + strand at 2525.316 kb on - strand at 2525.316 kb on - strand at 2525.316 kb on - strand at 2525.316 kb on - strand at 2525.369 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Gluconic Acid (C) remove 2,521,910 + Pf1N1B4_2208 0.64 -0.4 2,522,010 - Pf1N1B4_2208 0.69 +0.3 2,522,042 + Pf1N1B4_2208 0.70 +0.7 2,522,043 - Pf1N1B4_2208 0.70 +0.7 2,522,279 + Pf1N1B4_2208 0.81 +0.8 2,522,280 - Pf1N1B4_2208 0.81 +1.7 2,522,280 - Pf1N1B4_2208 0.81 +0.5 2,522,420 + Pf1N1B4_2208 0.87 +0.9 2,522,421 - Pf1N1B4_2208 0.87 +1.7 2,522,561 + +1.7 2,522,660 - +0.7 2,522,707 + -0.3 2,522,707 + +0.4 2,522,707 + +0.1 2,522,707 + +2.0 2,522,707 + +0.1 2,522,708 - +0.1 2,523,011 + -0.9 2,523,011 + -0.9 2,523,012 - -0.5 2,523,012 - +0.3 2,523,012 - +1.2 2,523,012 - -1.3 2,523,012 - -0.1 2,523,225 + Pf1N1B4_2209 0.23 -1.3 2,523,226 - Pf1N1B4_2209 0.23 -1.2 2,523,226 - Pf1N1B4_2209 0.23 -0.9 2,523,243 + Pf1N1B4_2209 0.24 +0.4 2,523,244 - Pf1N1B4_2209 0.24 -0.8 2,523,244 - Pf1N1B4_2209 0.24 +0.5 2,523,249 + Pf1N1B4_2209 0.25 -0.4 2,523,249 + Pf1N1B4_2209 0.25 -0.6 2,523,250 - Pf1N1B4_2209 0.25 -0.2 2,523,250 - Pf1N1B4_2209 0.25 -2.7 2,523,250 - Pf1N1B4_2209 0.25 +0.2 2,523,474 + Pf1N1B4_2209 0.40 -1.0 2,523,474 + Pf1N1B4_2209 0.40 +0.1 2,523,475 - Pf1N1B4_2209 0.40 -0.8 2,523,475 - Pf1N1B4_2209 0.40 +0.3 2,523,810 + Pf1N1B4_2209 0.62 -0.4 2,523,810 + Pf1N1B4_2209 0.62 -1.2 2,523,810 + Pf1N1B4_2209 0.62 -0.7 2,523,811 - Pf1N1B4_2209 0.62 -0.7 2,523,855 + Pf1N1B4_2209 0.65 -0.3 2,523,856 - Pf1N1B4_2209 0.65 -1.0 2,523,856 - Pf1N1B4_2209 0.65 -1.2 2,523,867 + Pf1N1B4_2209 0.66 -2.1 2,523,867 + Pf1N1B4_2209 0.66 -0.3 2,523,867 + Pf1N1B4_2209 0.66 -1.2 2,523,867 + Pf1N1B4_2209 0.66 -0.7 2,523,867 + Pf1N1B4_2209 0.66 -0.9 2,523,867 + Pf1N1B4_2209 0.66 -1.2 2,523,867 + Pf1N1B4_2209 0.66 -0.2 2,523,868 - Pf1N1B4_2209 0.66 -1.1 2,524,008 + Pf1N1B4_2209 0.75 -0.9 2,524,009 - Pf1N1B4_2209 0.75 -0.3 2,524,009 - Pf1N1B4_2209 0.75 -1.9 2,524,151 - Pf1N1B4_2209 0.85 -1.5 2,524,512 + -0.7 2,524,548 + -0.0 2,524,548 + +0.0 2,524,549 - -0.9 2,524,656 + +1.5 2,524,656 + +1.1 2,524,656 + -0.6 2,524,657 - +1.7 2,524,657 - -1.3 2,524,657 - -1.9 2,524,657 - -0.7 2,525,131 + Pf1N1B4_2210 0.73 -1.4 2,525,132 - Pf1N1B4_2210 0.73 +0.6 2,525,132 - -0.7 2,525,293 + +2.0 2,525,293 + -1.3 2,525,293 + +1.1 2,525,293 + +1.4 2,525,293 + -0.5 2,525,293 + +0.5 2,525,294 - +0.2 2,525,294 - +0.5 2,525,294 - -0.6 2,525,294 - -0.2 2,525,294 - -0.2 2,525,294 - +0.4 2,525,294 - -0.7 2,525,297 + +0.7 2,525,297 + +0.7 2,525,297 + -0.2 2,525,298 - -1.5 2,525,298 - -0.0 2,525,298 - -0.3 2,525,298 - +0.1 2,525,298 - +0.7 2,525,298 - -0.3 2,525,298 - +0.7 2,525,298 - +0.3 2,525,298 - +0.6 2,525,298 - +0.4 2,525,315 + +0.9 2,525,315 + -0.6 2,525,315 + -1.3 2,525,316 - -0.6 2,525,316 - -0.1 2,525,316 - +0.4 2,525,316 - +0.7 2,525,369 + +0.3
Or see this region's nucleotide sequence