Experiment: D-Gluconic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1661 and Pf1N1B4_1662 are separated by 3 nucleotides Pf1N1B4_1662 and Pf1N1B4_1663 are separated by 329 nucleotides
Pf1N1B4_1661 - Agmatine deiminase (EC 3.5.3.12), at 1,901,848 to 1,902,954
_1661
Pf1N1B4_1662 - N-carbamoylputrescine amidase (3.5.1.53) / Aliphatic amidase AmiE (EC 3.5.1.4), at 1,902,958 to 1,903,836
_1662
Pf1N1B4_1663 - 4-aminobutyrate aminotransferase (EC 2.6.1.19), at 1,904,166 to 1,907,078
_1663
Position (kb)
1902
1903
1904 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1902.044 kb on + strand, within Pf1N1B4_1661 at 1902.045 kb on - strand, within Pf1N1B4_1661 at 1902.045 kb on - strand, within Pf1N1B4_1661 at 1902.045 kb on - strand, within Pf1N1B4_1661 at 1902.045 kb on - strand, within Pf1N1B4_1661 at 1902.266 kb on - strand, within Pf1N1B4_1661 at 1902.266 kb on - strand, within Pf1N1B4_1661 at 1902.266 kb on - strand, within Pf1N1B4_1661 at 1902.266 kb on - strand, within Pf1N1B4_1661 at 1902.285 kb on - strand, within Pf1N1B4_1661 at 1902.349 kb on - strand, within Pf1N1B4_1661 at 1902.349 kb on - strand, within Pf1N1B4_1661 at 1902.349 kb on - strand, within Pf1N1B4_1661 at 1902.349 kb on - strand, within Pf1N1B4_1661 at 1902.670 kb on - strand, within Pf1N1B4_1661 at 1902.915 kb on - strand at 1902.915 kb on - strand at 1903.159 kb on - strand, within Pf1N1B4_1662 at 1903.159 kb on - strand, within Pf1N1B4_1662 at 1903.159 kb on - strand, within Pf1N1B4_1662 at 1903.159 kb on - strand, within Pf1N1B4_1662 at 1903.159 kb on - strand, within Pf1N1B4_1662 at 1903.159 kb on - strand, within Pf1N1B4_1662 at 1903.446 kb on - strand, within Pf1N1B4_1662 at 1903.446 kb on - strand, within Pf1N1B4_1662 at 1903.448 kb on + strand, within Pf1N1B4_1662 at 1903.489 kb on - strand, within Pf1N1B4_1662 at 1903.494 kb on - strand, within Pf1N1B4_1662 at 1903.566 kb on - strand, within Pf1N1B4_1662 at 1903.566 kb on - strand, within Pf1N1B4_1662 at 1903.566 kb on - strand, within Pf1N1B4_1662 at 1903.661 kb on + strand, within Pf1N1B4_1662 at 1903.662 kb on - strand, within Pf1N1B4_1662 at 1903.689 kb on - strand, within Pf1N1B4_1662 at 1903.977 kb on + strand at 1904.028 kb on - strand at 1904.090 kb on + strand at 1904.106 kb on + strand at 1904.106 kb on + strand at 1904.106 kb on + strand at 1904.106 kb on + strand at 1904.106 kb on + strand at 1904.106 kb on + strand at 1904.106 kb on + strand at 1904.107 kb on - strand at 1904.107 kb on - strand at 1904.107 kb on - strand at 1904.107 kb on - strand at 1904.107 kb on - strand at 1904.107 kb on - strand at 1904.107 kb on - strand at 1904.115 kb on - strand at 1904.167 kb on + strand at 1904.167 kb on + strand at 1904.168 kb on - strand at 1904.168 kb on - strand at 1904.168 kb on - strand at 1904.168 kb on - strand at 1904.168 kb on - strand at 1904.231 kb on + strand at 1904.231 kb on + strand at 1904.231 kb on + strand at 1904.232 kb on - strand at 1904.232 kb on - strand at 1904.384 kb on - strand at 1904.455 kb on + strand at 1904.567 kb on - strand, within Pf1N1B4_1663 at 1904.567 kb on - strand, within Pf1N1B4_1663 at 1904.567 kb on - strand, within Pf1N1B4_1663 at 1904.746 kb on - strand, within Pf1N1B4_1663 at 1904.773 kb on + strand, within Pf1N1B4_1663 at 1904.774 kb on - strand, within Pf1N1B4_1663 at 1904.774 kb on - strand, within Pf1N1B4_1663 at 1904.807 kb on + strand, within Pf1N1B4_1663
Per-strain Table
Position Strand Gene LocusTag Fraction D-Gluconic Acid (C) remove 1,902,044 + Pf1N1B4_1661 0.18 -0.5 1,902,045 - Pf1N1B4_1661 0.18 +0.7 1,902,045 - Pf1N1B4_1661 0.18 -0.5 1,902,045 - Pf1N1B4_1661 0.18 -2.6 1,902,045 - Pf1N1B4_1661 0.18 -0.5 1,902,266 - Pf1N1B4_1661 0.38 +0.5 1,902,266 - Pf1N1B4_1661 0.38 +1.1 1,902,266 - Pf1N1B4_1661 0.38 -0.7 1,902,266 - Pf1N1B4_1661 0.38 +0.8 1,902,285 - Pf1N1B4_1661 0.39 -0.6 1,902,349 - Pf1N1B4_1661 0.45 +0.1 1,902,349 - Pf1N1B4_1661 0.45 -1.9 1,902,349 - Pf1N1B4_1661 0.45 -1.3 1,902,349 - Pf1N1B4_1661 0.45 +0.4 1,902,670 - Pf1N1B4_1661 0.74 -0.1 1,902,915 - +0.7 1,902,915 - +0.2 1,903,159 - Pf1N1B4_1662 0.23 -1.5 1,903,159 - Pf1N1B4_1662 0.23 -1.2 1,903,159 - Pf1N1B4_1662 0.23 +1.7 1,903,159 - Pf1N1B4_1662 0.23 -0.6 1,903,159 - Pf1N1B4_1662 0.23 -1.7 1,903,159 - Pf1N1B4_1662 0.23 +0.3 1,903,446 - Pf1N1B4_1662 0.56 -1.8 1,903,446 - Pf1N1B4_1662 0.56 -2.3 1,903,448 + Pf1N1B4_1662 0.56 -1.3 1,903,489 - Pf1N1B4_1662 0.60 +0.5 1,903,494 - Pf1N1B4_1662 0.61 -1.4 1,903,566 - Pf1N1B4_1662 0.69 -0.9 1,903,566 - Pf1N1B4_1662 0.69 -0.5 1,903,566 - Pf1N1B4_1662 0.69 +0.7 1,903,661 + Pf1N1B4_1662 0.80 +0.5 1,903,662 - Pf1N1B4_1662 0.80 +1.1 1,903,689 - Pf1N1B4_1662 0.83 -0.6 1,903,977 + +1.7 1,904,028 - -0.2 1,904,090 + +0.1 1,904,106 + +0.7 1,904,106 + +1.0 1,904,106 + +1.0 1,904,106 + +1.8 1,904,106 + -1.2 1,904,106 + -0.5 1,904,106 + +0.9 1,904,107 - -0.1 1,904,107 - +0.7 1,904,107 - +0.2 1,904,107 - +2.1 1,904,107 - +0.1 1,904,107 - -0.2 1,904,107 - +1.1 1,904,115 - +1.4 1,904,167 + -0.2 1,904,167 + -0.3 1,904,168 - -0.9 1,904,168 - +0.8 1,904,168 - +0.7 1,904,168 - +0.7 1,904,168 - -1.0 1,904,231 + +0.5 1,904,231 + -1.2 1,904,231 + -0.3 1,904,232 - +0.7 1,904,232 - +1.7 1,904,384 - +0.3 1,904,455 + -0.8 1,904,567 - Pf1N1B4_1663 0.14 -0.7 1,904,567 - Pf1N1B4_1663 0.14 -0.4 1,904,567 - Pf1N1B4_1663 0.14 +0.1 1,904,746 - Pf1N1B4_1663 0.20 +0.8 1,904,773 + Pf1N1B4_1663 0.21 +1.7 1,904,774 - Pf1N1B4_1663 0.21 -0.2 1,904,774 - Pf1N1B4_1663 0.21 +0.2 1,904,807 + Pf1N1B4_1663 0.22 +1.4
Or see this region's nucleotide sequence