Experiment: D-Gluconic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1623 and Pf1N1B4_1624 are separated by 9 nucleotides Pf1N1B4_1624 and Pf1N1B4_1625 are separated by 99 nucleotides Pf1N1B4_1625 and Pf1N1B4_1626 are separated by 0 nucleotides Pf1N1B4_1626 and Pf1N1B4_1627 overlap by 1 nucleotides
Pf1N1B4_1623 - Imidazole glycerol phosphate synthase cyclase subunit (EC 4.1.3.-), at 1,867,729 to 1,868,499
_1623
Pf1N1B4_1624 - 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) (from data) , at 1,868,509 to 1,869,246
_1624
Pf1N1B4_1625 - FIG00956267: hypothetical protein, at 1,869,346 to 1,869,603
_1625
Pf1N1B4_1626 - Imidazole glycerol phosphate synthase amidotransferase subunit (EC 2.4.2.-), at 1,869,604 to 1,870,242
_1626
Pf1N1B4_1627 - Imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19), at 1,870,242 to 1,870,877
_1627
Position (kb)
1869
1870 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1868.355 kb on + strand, within Pf1N1B4_1623 at 1868.519 kb on + strand at 1868.519 kb on + strand at 1868.520 kb on - strand at 1868.520 kb on - strand at 1868.520 kb on - strand at 1868.520 kb on - strand at 1868.567 kb on + strand at 1868.567 kb on + strand at 1868.567 kb on + strand at 1868.567 kb on + strand at 1868.568 kb on - strand at 1868.568 kb on - strand at 1868.568 kb on - strand at 1868.568 kb on - strand at 1868.568 kb on - strand at 1868.568 kb on - strand at 1868.568 kb on - strand at 1868.702 kb on + strand, within Pf1N1B4_1624 at 1868.703 kb on - strand, within Pf1N1B4_1624 at 1868.703 kb on - strand, within Pf1N1B4_1624 at 1868.749 kb on - strand, within Pf1N1B4_1624 at 1868.750 kb on + strand, within Pf1N1B4_1624 at 1868.751 kb on - strand, within Pf1N1B4_1624 at 1868.948 kb on + strand, within Pf1N1B4_1624 at 1868.948 kb on + strand, within Pf1N1B4_1624 at 1868.948 kb on + strand, within Pf1N1B4_1624 at 1868.948 kb on + strand, within Pf1N1B4_1624 at 1868.948 kb on + strand, within Pf1N1B4_1624 at 1868.949 kb on - strand, within Pf1N1B4_1624 at 1868.949 kb on - strand, within Pf1N1B4_1624 at 1868.949 kb on - strand, within Pf1N1B4_1624 at 1868.969 kb on + strand, within Pf1N1B4_1624 at 1868.969 kb on + strand, within Pf1N1B4_1624 at 1868.970 kb on - strand, within Pf1N1B4_1624 at 1868.970 kb on - strand, within Pf1N1B4_1624 at 1868.970 kb on - strand, within Pf1N1B4_1624 at 1868.970 kb on - strand, within Pf1N1B4_1624 at 1869.052 kb on + strand, within Pf1N1B4_1624 at 1869.052 kb on + strand, within Pf1N1B4_1624 at 1869.052 kb on + strand, within Pf1N1B4_1624 at 1869.052 kb on + strand, within Pf1N1B4_1624 at 1869.052 kb on + strand, within Pf1N1B4_1624 at 1869.053 kb on - strand, within Pf1N1B4_1624 at 1869.053 kb on - strand, within Pf1N1B4_1624 at 1869.053 kb on - strand, within Pf1N1B4_1624 at 1869.219 kb on + strand at 1869.219 kb on + strand at 1869.237 kb on + strand at 1869.237 kb on + strand at 1869.237 kb on + strand at 1869.237 kb on + strand at 1869.238 kb on - strand at 1869.238 kb on - strand at 1869.241 kb on + strand at 1869.241 kb on + strand at 1869.241 kb on + strand at 1869.241 kb on + strand at 1869.241 kb on + strand at 1869.241 kb on + strand at 1869.241 kb on + strand at 1869.241 kb on + strand at 1869.242 kb on - strand at 1869.242 kb on - strand at 1869.242 kb on - strand at 1869.242 kb on - strand at 1869.242 kb on - strand at 1869.242 kb on - strand at 1869.282 kb on + strand at 1869.282 kb on + strand at 1869.282 kb on + strand at 1869.282 kb on + strand at 1869.282 kb on + strand at 1869.282 kb on + strand at 1869.282 kb on + strand at 1869.282 kb on + strand at 1869.282 kb on + strand at 1869.282 kb on + strand at 1869.282 kb on + strand at 1869.282 kb on + strand at 1869.282 kb on + strand at 1869.283 kb on - strand at 1869.283 kb on - strand at 1869.304 kb on + strand at 1869.347 kb on + strand at 1869.348 kb on - strand at 1869.434 kb on + strand, within Pf1N1B4_1625 at 1869.434 kb on + strand, within Pf1N1B4_1625 at 1869.434 kb on + strand, within Pf1N1B4_1625 at 1869.434 kb on + strand, within Pf1N1B4_1625 at 1869.434 kb on + strand, within Pf1N1B4_1625 at 1869.434 kb on + strand, within Pf1N1B4_1625 at 1869.434 kb on + strand, within Pf1N1B4_1625 at 1869.434 kb on + strand, within Pf1N1B4_1625 at 1869.435 kb on - strand, within Pf1N1B4_1625 at 1869.435 kb on - strand, within Pf1N1B4_1625 at 1869.435 kb on - strand, within Pf1N1B4_1625 at 1869.437 kb on + strand, within Pf1N1B4_1625 at 1869.438 kb on - strand, within Pf1N1B4_1625 at 1869.438 kb on - strand, within Pf1N1B4_1625 at 1869.606 kb on - strand at 1869.776 kb on + strand, within Pf1N1B4_1626 at 1869.776 kb on + strand, within Pf1N1B4_1626 at 1869.776 kb on + strand at 1869.776 kb on + strand, within Pf1N1B4_1626 at 1869.776 kb on + strand, within Pf1N1B4_1626 at 1869.776 kb on + strand, within Pf1N1B4_1626 at 1869.776 kb on + strand, within Pf1N1B4_1626 at 1869.776 kb on + strand, within Pf1N1B4_1626 at 1869.777 kb on - strand, within Pf1N1B4_1626 at 1869.777 kb on - strand, within Pf1N1B4_1626 at 1869.777 kb on - strand, within Pf1N1B4_1626 at 1869.777 kb on - strand, within Pf1N1B4_1626 at 1869.777 kb on - strand, within Pf1N1B4_1626 at 1869.777 kb on - strand, within Pf1N1B4_1626 at 1869.777 kb on - strand, within Pf1N1B4_1626 at 1869.777 kb on - strand, within Pf1N1B4_1626 at 1869.777 kb on - strand, within Pf1N1B4_1626 at 1869.777 kb on - strand, within Pf1N1B4_1626 at 1869.777 kb on - strand, within Pf1N1B4_1626 at 1869.777 kb on - strand, within Pf1N1B4_1626 at 1869.777 kb on - strand, within Pf1N1B4_1626 at 1869.779 kb on + strand, within Pf1N1B4_1626 at 1869.779 kb on + strand, within Pf1N1B4_1626 at 1869.779 kb on + strand at 1869.779 kb on + strand, within Pf1N1B4_1626 at 1869.779 kb on + strand, within Pf1N1B4_1626 at 1869.779 kb on + strand, within Pf1N1B4_1626 at 1869.779 kb on + strand, within Pf1N1B4_1626 at 1869.780 kb on - strand, within Pf1N1B4_1626 at 1869.780 kb on - strand, within Pf1N1B4_1626 at 1869.780 kb on - strand, within Pf1N1B4_1626 at 1869.780 kb on - strand, within Pf1N1B4_1626 at 1869.780 kb on - strand, within Pf1N1B4_1626 at 1869.795 kb on - strand, within Pf1N1B4_1626 at 1869.795 kb on - strand, within Pf1N1B4_1626 at 1869.795 kb on - strand, within Pf1N1B4_1626 at 1869.795 kb on - strand, within Pf1N1B4_1626 at 1869.795 kb on - strand, within Pf1N1B4_1626 at 1869.839 kb on - strand, within Pf1N1B4_1626 at 1869.839 kb on - strand, within Pf1N1B4_1626 at 1869.839 kb on - strand, within Pf1N1B4_1626 at 1870.466 kb on + strand, within Pf1N1B4_1627 at 1870.468 kb on + strand, within Pf1N1B4_1627 at 1870.468 kb on + strand, within Pf1N1B4_1627 at 1870.469 kb on - strand, within Pf1N1B4_1627 at 1870.469 kb on - strand, within Pf1N1B4_1627 at 1870.546 kb on + strand, within Pf1N1B4_1627 at 1870.546 kb on + strand, within Pf1N1B4_1627 at 1870.546 kb on + strand, within Pf1N1B4_1627 at 1870.547 kb on - strand, within Pf1N1B4_1627 at 1870.547 kb on - strand, within Pf1N1B4_1627 at 1870.547 kb on - strand, within Pf1N1B4_1627 at 1870.547 kb on - strand, within Pf1N1B4_1627
Per-strain Table
Position Strand Gene LocusTag Fraction D-Gluconic Acid (C) remove 1,868,355 + Pf1N1B4_1623 0.81 -1.0 1,868,519 + +0.6 1,868,519 + -2.0 1,868,520 - +0.9 1,868,520 - -2.1 1,868,520 - -1.8 1,868,520 - -0.1 1,868,567 + -1.4 1,868,567 + -0.0 1,868,567 + -3.2 1,868,567 + -1.7 1,868,568 - -1.0 1,868,568 - -0.5 1,868,568 - -2.6 1,868,568 - -0.4 1,868,568 - -2.1 1,868,568 - -2.0 1,868,568 - -2.6 1,868,702 + Pf1N1B4_1624 0.26 -0.7 1,868,703 - Pf1N1B4_1624 0.26 -1.4 1,868,703 - Pf1N1B4_1624 0.26 +0.0 1,868,749 - Pf1N1B4_1624 0.33 +0.6 1,868,750 + Pf1N1B4_1624 0.33 -2.5 1,868,751 - Pf1N1B4_1624 0.33 -2.1 1,868,948 + Pf1N1B4_1624 0.59 -3.3 1,868,948 + Pf1N1B4_1624 0.59 -1.4 1,868,948 + Pf1N1B4_1624 0.59 -1.0 1,868,948 + Pf1N1B4_1624 0.59 -1.5 1,868,948 + Pf1N1B4_1624 0.59 -1.7 1,868,949 - Pf1N1B4_1624 0.60 -3.3 1,868,949 - Pf1N1B4_1624 0.60 -1.0 1,868,949 - Pf1N1B4_1624 0.60 -0.7 1,868,969 + Pf1N1B4_1624 0.62 -2.7 1,868,969 + Pf1N1B4_1624 0.62 -2.8 1,868,970 - Pf1N1B4_1624 0.62 -4.5 1,868,970 - Pf1N1B4_1624 0.62 -1.8 1,868,970 - Pf1N1B4_1624 0.62 -2.0 1,868,970 - Pf1N1B4_1624 0.62 -1.2 1,869,052 + Pf1N1B4_1624 0.74 -0.7 1,869,052 + Pf1N1B4_1624 0.74 -0.7 1,869,052 + Pf1N1B4_1624 0.74 -1.2 1,869,052 + Pf1N1B4_1624 0.74 -2.6 1,869,052 + Pf1N1B4_1624 0.74 -1.5 1,869,053 - Pf1N1B4_1624 0.74 -3.3 1,869,053 - Pf1N1B4_1624 0.74 -1.8 1,869,053 - Pf1N1B4_1624 0.74 -0.4 1,869,219 + -1.5 1,869,219 + -1.7 1,869,237 + -1.4 1,869,237 + -1.7 1,869,237 + +0.3 1,869,237 + -1.6 1,869,238 - +0.2 1,869,238 - -1.7 1,869,241 + -1.2 1,869,241 + -0.7 1,869,241 + -0.9 1,869,241 + -2.2 1,869,241 + -0.2 1,869,241 + -1.8 1,869,241 + -1.9 1,869,241 + -1.5 1,869,242 - -0.9 1,869,242 - -2.4 1,869,242 - -2.1 1,869,242 - -2.8 1,869,242 - -2.9 1,869,242 - -1.2 1,869,282 + -2.7 1,869,282 + -3.2 1,869,282 + -2.7 1,869,282 + -2.2 1,869,282 + -2.7 1,869,282 + -2.4 1,869,282 + -1.3 1,869,282 + -1.2 1,869,282 + -2.4 1,869,282 + -1.7 1,869,282 + -2.5 1,869,282 + -1.3 1,869,282 + +0.6 1,869,283 - +1.3 1,869,283 - +1.8 1,869,304 + -0.7 1,869,347 + -3.2 1,869,348 - +0.5 1,869,434 + Pf1N1B4_1625 0.34 +0.6 1,869,434 + Pf1N1B4_1625 0.34 -2.1 1,869,434 + Pf1N1B4_1625 0.34 -2.9 1,869,434 + Pf1N1B4_1625 0.34 -3.2 1,869,434 + Pf1N1B4_1625 0.34 -2.7 1,869,434 + Pf1N1B4_1625 0.34 -1.5 1,869,434 + Pf1N1B4_1625 0.34 -2.1 1,869,434 + Pf1N1B4_1625 0.34 +0.6 1,869,435 - Pf1N1B4_1625 0.34 +0.9 1,869,435 - Pf1N1B4_1625 0.34 -0.9 1,869,435 - Pf1N1B4_1625 0.34 -0.1 1,869,437 + Pf1N1B4_1625 0.35 -0.4 1,869,438 - Pf1N1B4_1625 0.36 -0.1 1,869,438 - Pf1N1B4_1625 0.36 +2.2 1,869,606 - -0.2 1,869,776 + Pf1N1B4_1626 0.27 -2.2 1,869,776 + Pf1N1B4_1626 0.27 -0.9 1,869,776 + -0.4 1,869,776 + Pf1N1B4_1626 0.27 -1.4 1,869,776 + Pf1N1B4_1626 0.27 -2.9 1,869,776 + Pf1N1B4_1626 0.27 -0.4 1,869,776 + Pf1N1B4_1626 0.27 -0.9 1,869,776 + Pf1N1B4_1626 0.27 -1.9 1,869,777 - Pf1N1B4_1626 0.27 -0.7 1,869,777 - Pf1N1B4_1626 0.27 -1.4 1,869,777 - Pf1N1B4_1626 0.27 -2.9 1,869,777 - Pf1N1B4_1626 0.27 -0.7 1,869,777 - Pf1N1B4_1626 0.27 +0.9 1,869,777 - Pf1N1B4_1626 0.27 -2.7 1,869,777 - Pf1N1B4_1626 0.27 -3.2 1,869,777 - Pf1N1B4_1626 0.27 -0.4 1,869,777 - Pf1N1B4_1626 0.27 -2.5 1,869,777 - Pf1N1B4_1626 0.27 -0.9 1,869,777 - Pf1N1B4_1626 0.27 -2.5 1,869,777 - Pf1N1B4_1626 0.27 +0.6 1,869,777 - Pf1N1B4_1626 0.27 -1.9 1,869,779 + Pf1N1B4_1626 0.27 -1.7 1,869,779 + Pf1N1B4_1626 0.27 -1.7 1,869,779 + +0.1 1,869,779 + Pf1N1B4_1626 0.27 -1.2 1,869,779 + Pf1N1B4_1626 0.27 +0.1 1,869,779 + Pf1N1B4_1626 0.27 -1.2 1,869,779 + Pf1N1B4_1626 0.27 -1.8 1,869,780 - Pf1N1B4_1626 0.28 -1.0 1,869,780 - Pf1N1B4_1626 0.28 -1.5 1,869,780 - Pf1N1B4_1626 0.28 -1.8 1,869,780 - Pf1N1B4_1626 0.28 -0.4 1,869,780 - Pf1N1B4_1626 0.28 -2.2 1,869,795 - Pf1N1B4_1626 0.30 -2.4 1,869,795 - Pf1N1B4_1626 0.30 -1.3 1,869,795 - Pf1N1B4_1626 0.30 -1.7 1,869,795 - Pf1N1B4_1626 0.30 -1.6 1,869,795 - Pf1N1B4_1626 0.30 -0.9 1,869,839 - Pf1N1B4_1626 0.37 -1.4 1,869,839 - Pf1N1B4_1626 0.37 -2.9 1,869,839 - Pf1N1B4_1626 0.37 -0.7 1,870,466 + Pf1N1B4_1627 0.35 -3.0 1,870,468 + Pf1N1B4_1627 0.36 -3.8 1,870,468 + Pf1N1B4_1627 0.36 -1.2 1,870,469 - Pf1N1B4_1627 0.36 -0.9 1,870,469 - Pf1N1B4_1627 0.36 -2.5 1,870,546 + Pf1N1B4_1627 0.48 -1.7 1,870,546 + Pf1N1B4_1627 0.48 -1.9 1,870,546 + Pf1N1B4_1627 0.48 -1.7 1,870,547 - Pf1N1B4_1627 0.48 -0.4 1,870,547 - Pf1N1B4_1627 0.48 -2.3 1,870,547 - Pf1N1B4_1627 0.48 -1.9 1,870,547 - Pf1N1B4_1627 0.48 -1.9
Or see this region's nucleotide sequence