Experiment: D-Gluconic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1610 and Pf1N1B4_1611 overlap by 4 nucleotides Pf1N1B4_1611 and Pf1N1B4_1612 are separated by 630 nucleotides
Pf1N1B4_1610 - Nitrogen regulation protein NtrB (EC 2.7.13.3), at 1,854,741 to 1,855,826
_1610
Pf1N1B4_1611 - Nitrogen regulation protein NR(I), at 1,855,823 to 1,857,259
_1611
Pf1N1B4_1612 - FIG00955429: hypothetical protein, at 1,857,890 to 1,858,330
_1612
Position (kb)
1855
1856
1857
1858 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1854.828 kb on + strand at 1854.829 kb on - strand at 1854.829 kb on - strand at 1854.829 kb on - strand at 1854.829 kb on - strand at 1854.829 kb on - strand at 1854.829 kb on - strand at 1854.829 kb on - strand at 1854.829 kb on - strand at 1854.829 kb on - strand at 1854.829 kb on - strand at 1854.829 kb on - strand at 1854.829 kb on - strand at 1854.875 kb on + strand, within Pf1N1B4_1610 at 1855.020 kb on + strand, within Pf1N1B4_1610 at 1855.021 kb on - strand, within Pf1N1B4_1610 at 1855.021 kb on - strand, within Pf1N1B4_1610 at 1855.053 kb on - strand, within Pf1N1B4_1610 at 1855.248 kb on + strand, within Pf1N1B4_1610 at 1855.367 kb on - strand, within Pf1N1B4_1610 at 1855.367 kb on - strand, within Pf1N1B4_1610 at 1855.367 kb on - strand, within Pf1N1B4_1610 at 1855.419 kb on + strand, within Pf1N1B4_1610 at 1855.867 kb on + strand at 1855.868 kb on - strand at 1855.868 kb on - strand at 1855.868 kb on - strand at 1856.106 kb on - strand, within Pf1N1B4_1611 at 1856.126 kb on + strand, within Pf1N1B4_1611 at 1856.126 kb on + strand, within Pf1N1B4_1611 at 1856.127 kb on - strand, within Pf1N1B4_1611 at 1856.127 kb on - strand, within Pf1N1B4_1611 at 1856.233 kb on + strand at 1856.609 kb on + strand, within Pf1N1B4_1611 at 1856.609 kb on + strand, within Pf1N1B4_1611 at 1856.610 kb on - strand, within Pf1N1B4_1611 at 1856.610 kb on - strand, within Pf1N1B4_1611 at 1856.864 kb on + strand, within Pf1N1B4_1611 at 1856.864 kb on + strand, within Pf1N1B4_1611 at 1856.864 kb on + strand, within Pf1N1B4_1611 at 1856.865 kb on - strand, within Pf1N1B4_1611 at 1857.184 kb on + strand at 1857.184 kb on + strand at 1857.185 kb on - strand at 1857.185 kb on - strand at 1857.185 kb on - strand at 1857.185 kb on - strand at 1857.185 kb on - strand at 1857.257 kb on + strand at 1857.257 kb on + strand at 1857.257 kb on + strand at 1857.257 kb on + strand at 1857.257 kb on + strand at 1857.257 kb on + strand at 1857.258 kb on - strand at 1857.262 kb on - strand at 1857.626 kb on + strand at 1857.627 kb on - strand at 1857.627 kb on - strand at 1857.653 kb on + strand at 1857.653 kb on + strand at 1857.653 kb on + strand at 1857.654 kb on - strand at 1857.654 kb on - strand at 1857.654 kb on - strand at 1857.654 kb on - strand at 1857.654 kb on - strand at 1857.708 kb on + strand at 1857.708 kb on + strand at 1857.734 kb on + strand at 1857.734 kb on + strand at 1857.734 kb on + strand at 1857.812 kb on + strand at 1857.812 kb on + strand at 1857.812 kb on + strand at 1857.813 kb on - strand at 1857.813 kb on - strand at 1857.813 kb on - strand at 1857.864 kb on + strand at 1857.865 kb on - strand at 1857.891 kb on + strand at 1857.891 kb on + strand at 1857.891 kb on + strand at 1857.891 kb on + strand at 1857.891 kb on + strand at 1857.891 kb on + strand at 1857.891 kb on + strand at 1857.892 kb on - strand at 1857.892 kb on - strand at 1857.892 kb on - strand at 1858.079 kb on + strand, within Pf1N1B4_1612 at 1858.080 kb on - strand, within Pf1N1B4_1612 at 1858.080 kb on - strand, within Pf1N1B4_1612
Per-strain Table
Position Strand Gene LocusTag Fraction D-Gluconic Acid (C) remove 1,854,828 + +1.6 1,854,829 - -1.6 1,854,829 - -1.5 1,854,829 - -2.0 1,854,829 - -0.1 1,854,829 - -0.7 1,854,829 - +0.0 1,854,829 - -0.7 1,854,829 - -2.4 1,854,829 - -2.8 1,854,829 - -1.7 1,854,829 - -0.4 1,854,829 - -1.2 1,854,875 + Pf1N1B4_1610 0.12 -2.3 1,855,020 + Pf1N1B4_1610 0.26 +0.0 1,855,021 - Pf1N1B4_1610 0.26 -1.3 1,855,021 - Pf1N1B4_1610 0.26 -1.6 1,855,053 - Pf1N1B4_1610 0.29 -2.0 1,855,248 + Pf1N1B4_1610 0.47 +0.0 1,855,367 - Pf1N1B4_1610 0.58 +0.0 1,855,367 - Pf1N1B4_1610 0.58 -0.4 1,855,367 - Pf1N1B4_1610 0.58 -1.8 1,855,419 + Pf1N1B4_1610 0.62 +0.1 1,855,867 + -1.0 1,855,868 - -1.0 1,855,868 - +0.0 1,855,868 - -0.8 1,856,106 - Pf1N1B4_1611 0.20 -1.1 1,856,126 + Pf1N1B4_1611 0.21 -1.2 1,856,126 + Pf1N1B4_1611 0.21 -1.2 1,856,127 - Pf1N1B4_1611 0.21 -1.8 1,856,127 - Pf1N1B4_1611 0.21 -2.0 1,856,233 + -1.5 1,856,609 + Pf1N1B4_1611 0.55 -0.2 1,856,609 + Pf1N1B4_1611 0.55 -0.8 1,856,610 - Pf1N1B4_1611 0.55 +0.2 1,856,610 - Pf1N1B4_1611 0.55 -1.0 1,856,864 + Pf1N1B4_1611 0.72 -0.2 1,856,864 + Pf1N1B4_1611 0.72 -1.5 1,856,864 + Pf1N1B4_1611 0.72 -0.7 1,856,865 - Pf1N1B4_1611 0.73 +0.3 1,857,184 + -2.2 1,857,184 + -2.7 1,857,185 - -1.2 1,857,185 - -2.0 1,857,185 - -1.6 1,857,185 - +0.6 1,857,185 - -0.7 1,857,257 + +0.8 1,857,257 + -0.5 1,857,257 + +2.6 1,857,257 + -0.4 1,857,257 + +0.9 1,857,257 + -1.1 1,857,258 - +0.3 1,857,262 - +0.0 1,857,626 + -0.4 1,857,627 - +1.6 1,857,627 - +1.9 1,857,653 + +1.0 1,857,653 + -1.2 1,857,653 + -1.0 1,857,654 - -2.4 1,857,654 - +0.3 1,857,654 - -1.2 1,857,654 - -0.2 1,857,654 - +1.6 1,857,708 + -0.2 1,857,708 + +0.4 1,857,734 + +0.1 1,857,734 + +2.0 1,857,734 + -1.2 1,857,812 + +1.2 1,857,812 + +0.5 1,857,812 + -1.5 1,857,813 - +0.6 1,857,813 - +1.6 1,857,813 - -0.8 1,857,864 + -0.6 1,857,865 - -0.8 1,857,891 + +0.4 1,857,891 + -0.5 1,857,891 + -0.0 1,857,891 + -0.5 1,857,891 + +0.1 1,857,891 + -1.5 1,857,891 + +0.4 1,857,892 - +0.6 1,857,892 - +1.2 1,857,892 - -0.5 1,858,079 + Pf1N1B4_1612 0.43 +0.4 1,858,080 - Pf1N1B4_1612 0.43 -0.2 1,858,080 - Pf1N1B4_1612 0.43 -0.8
Or see this region's nucleotide sequence